@text

EXERCISE 13. PHOSPHOFRUCTOKINASE - ALLOSTERIC INTERACTIONS

KINEMAGE 1: Conformational Changes in a Dimeric Unit of PFK.
KINEMAGE 2: The Major Conformational Changes in a Subunit of PFK.

	Phosphofructokinase (PFK) is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from ATP to fructose-6-phosphate (F6P) to yield ADP and fructose-1,6-bisphosphate (FBP). The PFK reaction is strongly exergonic (irreversible) under physiological conditions (Table 14-1) and hence is one of the glycolytic pathway's rate-determining steps. In most organisms/tissues, PFK is the glycolytic pathway's major flux-controlling enzyme; its activity is controlled by the concentrations of an unusually large number of metabolites including ATP, ADP, AMP, and fructose-2,6-bisphosphate (F2,6P). 
	The symmetry model of allosterism requires that an oligomeric (multisubunit) protein maintain its molecular symmetry in undergoing a transition from its high-activity R state to its low-activity T state. Hence, this transition must be concerted, with no intermediate states. The X-ray and enzymological evidence indicates that PFK, a tetramer of identical subunits, is an allosteric enzyme that follows the symmetry model. Hence, the binding of one molecule of its substrate F6P, which binds to the R state enzyme with high affinity but to the T state enzyme with low affinity, causes PFK to take up the R state, which in turn, increases the binding affinity of the enzyme for additional F6P (a homotropic effect). Activators, such as ADP and AMP bind to so-called allosteric sites, binding sites distinct from the active site, where they likewise facilitate the formation of the R state and hence activate the enzyme (a heterotropic effect; ADP, being a product of the PFK reaction, also binds at the enzyme's active site). Similarly, inhibitors such as PEP bind to allosteric sites (which in the case of PFK overlaps the activating allosteric site) where they promote the formation of the T state, thereby inhibiting the enzyme.

	This exercise consists of two kinemages that illustrate some of the allosterically-induced conformational changes that occur in PFK from Bacillus stearothermophilus.

	If you have not already done so, select EXERCISE 13: Phosphofructokinase (PFK) from among the exercises listed in the appropriate chapter in the "Kinemages" section of the Student Companion site for Fundamentals of Biochemistry, 2nd Edition at www.wiley.com/college/voet. This will open the exercise in the Java-based version of KiNG (Kinemage, New Generation).

KINEMAGE 1: Conformational Changes in a Dimeric Unit of PFK.

	PFK from B. stearothermophilus is a tetramer of identical 320-residue subunits. This kinemage shows the same two subunits of the tetramer as does. View1 shows the two subunits in their R state conformation as represented by their Ca backbones with Subunit 1 in pinktint and Subunit 2 in pink. Two side chains in each subunit are shown, those of Glu 161 (red) and Arg 162 (cyan), which are important participants in PFK's allosterically facilitated conformational change (see below). An F6P (hotpink) and an ADP (green; "ADP-active") are bound in the active site of each subunit. An additional ADP (yellow; "ADP-allo") is bound in a separate so-called allosteric site of each subunit. The ADPs each have an associated Mg2+, which is represented here by a ball of the same color as the ADP to which it binds.

	Click the "ANIMATE" button to switch the dimer between its R and T states. In its T state, Subunit 1 is bluetint and Subunit 2 is skyblue. The side chains of Glu 161 and Arg 162 in both subunits are red and cyan as before (only the Ca and Cb atoms of the Arg 162 side chain in Subunit 1 are observed in the X-ray structure of the T state; those of Subunit 2 are all observed). The T state enzyme binds the inhibitor 2-phosphoglycolate (gold; "PGC"), a nonphysiological analog of the glycolytic intermediate phosphoenolpyruvate (PEP). Note that the binding site of PGC in the T state overlaps the allosteric binding site of ADP in the R state ("ADP-allo") and hence their binding is mutually exclusive. The T state active sites, which do not contain F6P, are marked by "ghost" F6Ps (gray;"F6P site"), which have the same positions as do the F6Ps in the R state enzyme.

	View2 is a closeup of the upper portion of View1 showing both the active site and the allosteric site in this region. Note that the active site is located at the interface between two subunits and that the allosteric site interacts directly with the adjacent active site. Compare the R state and T state conformations by displaying both at once or clicking on "ANIMATE". Can you identify the Mg2+ ion associated with each of the ADPs bound to the enzyme in the R state? Which ADP atoms coordinate these Mg2+ ions? 

	The phosphate group of PGC binds to the allosteric site in the T state in very nearly the same position that the beta phosphate group of "ADP-allo" binds to the R state allosteric site; both phosphate groups bind to the side chains of the same three Arg residues (not shown).

	In the high-activity R state, the positively charged side chain of Arg 162 forms a hydrogen bonded salt bridge with the negatively charged 6-phosphate group of F6P (white dashed lines), an interaction which presumably stabilizes the R state relative to the T state and is therefore in part responsible for F6P's homotropic effect.

KINEMAGE 2: The Major Conformational Changes in a Subunit of PFK.

	KINEMAGE 2 shows those segments near the allosteric site (residues 53-60 are not shown here). As in KINEMAGE 1, the polypeptide is represented by its Ca chain with R state Subunits 1 and 2 in redtint and pink, and T state Subunits 1 and 2 in bluetint and skyblue. KINEMAGE 2 comes up in the R state showing the phosphate group of F6P (hotpink) bound in the enzyme's active site in a hydrogen bonded salt bridge (dashed white lines) with the side chain of Arg 162 (cyan). An ADP (yellow; "ADP-allo") occupies the adjacent allosteric site. Click once on "ANIMATE" to switch to the T state. This replaces the ADP in the R state allosteric site with the inhibitor and PEP analog PGC (gold). F6P no longer occupies the active site but its position in the R state is indicated by the "ghost" F6P (gray; "F6P site"). 

	What happens to the central polypeptide helical segment (residues 149-164) in the R to T transition? What does this do to the relative positions of the negatively charged Glu 161 and the positively charged Arg 162? Click on "F6P site". What influence would the presence of the carboxylate group of Glu 161 have on the phosphate group of F6P were it to bind in the active site? Does this explain, at least in part, why T state PFK has low affinity for F6P? Go to View2 for a closeup of the F6P-sidechain interactions.

	
The atomic coordinates for R state PFK were obtained from 4PFK; those for T state PFK were obtained from Philip Evans, MRC Laboratory of Molecular Biology, Cambridge, U. K.





@kinemage 1 
@caption
R and T state phosphofructokinase PFK). R state subunits are pinktint and pink, and T state subunits are bluetint and skyblue. In the R state, F6P (hotpink) is bound to the active site, and ADP is bound both to the active site (green) and the allosteric site (yellow). In the T state, the allosteric inhibitor 2-phosphoglycolate (PGC; a PEP analog) is bound to the allosteric site (gold). The F6P-binding site in the T state, which does not bind PFK, is marked by the position of the F6P in the R state (gray). The side chains of Glu 161 and Arg 162 are red and cyan.
@onewidth 
@perspective 
@viewid {PFK Dimer}
@zoom 1.00 
@zslab 115 
@center -4.049 -4.662 -17.831 
@matrix 
-0.973383 0.223897 -0.048948 0.223042 0.974553 0.022346 0.052706 0.010834 -0.998551 
@2viewid {Allo/Act Sites }
@2zoom 2.59 
@2zslab 169 
@2center 6.041 12.440 -5.018 
@2matrix 
-0.973383 0.223897 -0.048948 0.223042 0.974553 0.022346 0.052706 0.010834 -0.998551 
@group {R-State}  animate   
@subgroup {Subunit 1}   dominant 
@vectorlist {ca-ca} color= pinktint a     
{ ca  met a   1 } P 28.049, 32.245, 0.597 
{ ca  met a   1 } 28.049, 32.245, 0.597 
{ ca  lys a   2 } 24.804, 33.691, 1.903 
{ ca  arg a   3 } 22.400, 32.832, -0.881 
{ ca  ile a   4 } 21.914, 29.621, -2.901 
{ ca  gly a   5 } 19.386, 28.380, -5.423 
{ ca  val a   6 } 17.842, 24.904, -5.901 
{ ca  leu a   7 } 16.018, 23.467, -8.906 
{ ca  thr a   8 } 14.712, 20.197, -10.320 
{ ca  ser a   9 } 15.347, 19.178, -13.961 
{ ca  gly a   10 } 14.841, 16.122, -16.148 
{ ca  gly a   11 } 12.047, 13.660, -15.452 
{ ca  asp a   12 } 10.613, 13.933, -11.942 
{ ca  ser a   13 } 11.243, 11.205, -9.415 
{ ca  pro a   14 } 9.778, 10.231, -6.043 
{ ca  gly a   15 } 12.090, 11.777, -3.430 
{ ca  met a   16 } 12.824, 15.070, -5.221 
{ ca  asn a   17 } 10.846, 16.878, -2.481 
{ ca  ala a   18 } 12.791, 15.242, 0.393 
{ ca  ala a   19 } 15.889, 16.481, -1.473 
{ ca  ile a   20 } 14.568, 20.037, -1.822 
{ ca  arg a   21 } 13.657, 19.847, 1.845 
{ ca  ser a   22 } 17.264, 18.935, 3.052 
{ ca  val a   23 } 18.861, 21.555, 0.790 
{ ca  val a   24 } 16.438, 24.110, 2.253 
{ ca  arg a   25 } 16.868, 22.998, 5.860 
{ ca  lys a   26 } 20.567, 22.269, 5.564 
{ ca  ala a   27 } 21.222, 25.792, 4.133 
{ ca  ile a   28 } 19.142, 27.637, 6.794 
{ ca  tyr a   29 } 20.715, 25.715, 9.699 
{ ca  his a   30 } 23.862, 27.216, 8.328 
{ ca  gly a   31 } 22.256, 30.653, 8.160 
{ ca  val a   32 } 22.359, 30.673, 4.352 
{ ca  glu a   33 } 19.188, 31.678, 2.483 
{ ca  val a   34 } 17.572, 29.468, -0.168 
{ ca  tyr a   35 } 15.895, 30.473, -3.402 
{ ca  gly a   36 } 13.799, 28.147, -5.503 
{ ca  val a   37 } 13.929, 28.070, -9.295 
{ ca  tyr a   38 } 10.578, 26.884, -10.753 
{ ca  his a   39 } 10.417, 24.970, -14.028 
{ ca  gly a   40 } 14.079, 23.970, -13.910 
{ ca  tyr a   41 } 16.404, 25.565, -16.472 
{ ca  ala a   42 } 13.479, 27.081, -18.344 
{ ca  gly a   43 } 12.393, 29.191, -15.373 
{ ca  leu a   44 } 16.002, 29.941, -14.576 
{ ca  ile a   45 } 16.118, 31.968, -17.775 
{ ca  ala a   46 } 12.594, 33.384, -17.297 
{ ca  gly a   47 } 13.609, 34.397, -13.753 
{ ca  asn a   48 } 10.908, 32.254, -12.053 
{ ca  ile a   49 } 12.693, 32.478, -8.651 
{ ca  lys a   50 } 11.072, 32.660, -5.149 
{ ca  lys a   51 } 12.679, 32.576, -1.672 
{ ca  leu a   52 } 11.999, 29.329, 0.271 
{ ca  glu a   53 } 11.324, 29.740, 3.994 
{ ca  val a   54 } 11.830, 26.993, 6.583 
{ ca  gly a   55 } 8.107, 26.005, 6.473 
{ ca  asp a   56 } 7.579, 26.008, 2.723 
{ ca  val a   57 } 9.255, 22.571, 2.808 
{ ca  gly a   58 } 7.149, 21.404, 5.712 
{ ca  asp a   59 } 4.821, 18.601, 4.628 
{ ca  ile a   60 } 6.501, 17.614, 1.402 
{ ca  ile a   61 } 8.773, 14.847, 2.734 
{ ca  his a   62 } 6.290, 12.054, 1.933 
{ ca  arg a   63 } 5.225, 13.459, -1.460 
{ ca  gly a   64 } 6.041, 12.440, -5.018 
{ ca  gly a   65 } 6.913, 14.793, -7.904 
{ ca  thr a   66 } 8.636, 18.109, -7.295 
{ ca  ile a   67 } 7.041, 21.184, -5.782 
{ ca  leu a   68 } 9.425, 23.358, -7.921 
{ ca  tyr a   69 } 8.196, 21.713, -11.147 
{ ca  thr a   70 } 10.481, 20.729, -14.002 
{ ca  ala a   71 } 10.768, 21.640, -17.657 
{ ca  arg a   72 } 12.811, 20.938, -20.763 
{ ca  cys a   73 } 14.997, 23.961, -21.734 
{ ca  pro a   74 } 16.722, 23.473, -25.101 
{ ca  glu a   75 } 17.599, 27.216, -24.978 
{ ca  phe a   76 } 20.006, 26.512, -22.051 
{ ca  lys a   77 } 22.303, 24.583, -24.395 
{ ca  thr a   78 } 22.949, 27.978, -25.975 
{ ca  glu a   79 } 25.557, 30.475, -24.821 
{ ca  glu a   80 } 22.899, 33.188, -24.728 
{ ca  gly a   81 } 20.746, 30.819, -22.656 
{ ca  gln a   82 } 23.453, 30.195, -20.093 
{ ca  lys a   83 } 24.026, 33.979, -20.174 
{ ca  lys a   84 } 20.415, 34.952, -19.582 
{ ca  gly a   85 } 20.130, 32.400, -16.781 
{ ca  ile a   86 } 23.446, 33.502, -15.290 
{ ca  glu a   87 } 22.093, 37.068, -15.308 
{ ca  gln a   88 } 18.958, 35.989, -13.501 
{ ca  leu a   89 } 20.986, 34.438, -10.737 
{ ca  lys a   90 } 22.977, 37.634, -10.212 
{ ca  lys a   91 } 19.908, 39.815, -10.484 
{ ca  his a   92 } 18.680, 37.819, -7.454 
{ ca  gly a   93 } 22.061, 37.692, -5.716 
{ ca  ile a   94 } 22.213, 33.911, -5.859 
{ ca  gln a   95 } 25.753, 32.595, -5.596 
{ ca  gly a   96 } 25.402, 28.779, -5.694 
{ ca  leu a   97 } 23.105, 26.241, -7.228 
{ ca  val a   98 } 21.929, 22.823, -6.138 
{ ca  val a   99 } 20.595, 21.016, -9.176 
{ ca  ile a   100 } 18.597, 17.785, -8.499 
{ ca  gly a   101 } 18.106, 15.763, -11.728 
{ ca  gly a   102 } 19.320, 13.578, -14.568 
{ ca  asp a   103 } 22.531, 13.469, -16.573 
{ ca  gly a   104 } 21.085, 15.936, -19.094 
{ ca  ser a   105 } 20.699, 18.193, -16.107 
{ ca  tyr a   106 } 24.233, 17.603, -14.760 
{ ca  gln a   107 } 25.530, 18.873, -18.122 
{ ca  gly a   108 } 23.984, 22.286, -17.399 
{ ca  ala a   109 } 25.397, 22.353, -13.874 
{ ca  lys a   110 } 28.775, 21.860, -15.582 
{ ca  lys a   111 } 28.267, 24.697, -18.133 
{ ca  leu a   112 } 27.320, 26.880, -15.173 
{ ca  thr a   113 } 30.529, 26.322, -13.141 
{ ca  glu a   114 } 32.636, 26.915, -16.294 
{ ca  his a   115 } 30.975, 30.387, -16.390 
{ ca  gly a   116 } 31.619, 30.827, -12.652 
{ ca  phe a   117 } 28.534, 29.515, -10.879 
{ ca  pro a   118 } 29.269, 26.895, -8.270 
{ ca  cys a   119 } 26.806, 23.997, -8.753 
{ ca  val a   120 } 26.356, 20.536, -7.280 
{ ca  gly a   121 } 24.208, 17.873, -8.955 
{ ca  val a   122 } 21.915, 15.570, -6.908 
{ ca  pro a   123 } 20.971, 12.373, -8.786 
{ ca  gly a   124 } 17.206, 12.362, -9.292 
{ ca  thr a   125 } 15.902, 9.775, -11.786 
{ ca  ile a   126 } 13.952, 6.511, -11.365 
{ ca  asp a   127 } 16.298, 4.893, -13.896 
{ ca  asn a   128 } 19.396, 4.545, -11.674 
{ ca  asp a   129 } 21.520, 5.508, -14.724 
{ ca  ile a   130 } 23.677, 8.241, -13.235 
{ ca  pro a   131 } 27.431, 7.822, -12.813 
{ ca  gly a   132 } 29.004, 8.570, -9.456 
{ ca  thr a   133 } 26.154, 6.858, -7.568 
{ ca  asp a   134 } 25.103, 3.224, -6.903 
{ ca  phe a   135 } 21.628, 4.478, -6.339 
{ ca  thr a   136 } 19.728, 7.511, -7.693 
{ ca  ile a   137 } 16.845, 9.195, -5.782 
{ ca  gly a   138 } 13.529, 7.587, -6.812 
{ ca  phe a   139 } 14.850, 4.196, -7.995 
{ ca  asp a   140 } 13.808, 2.355, -4.778 
{ ca  thr a   141 } 10.370, 3.867, -5.160 
{ ca  ala a   142 } 9.958, 2.794, -8.830 
{ ca  leu a   143 } 11.095, -0.685, -7.896 
{ ca  asn a   144 } 8.483, -1.067, -5.248 
{ ca  thr a   145 } 5.914, 0.367, -7.714 
{ ca  val a   146 } 6.832, -2.297, -10.238 
{ ca  ile a   147 } 6.850, -5.352, -7.883 
{ ca  asp a   148 } 3.517, -4.053, -6.679 
{ ca  ala a   149 } 2.412, -4.539, -10.288 
{ ca  ile a   150 } 4.102, -7.918, -10.677 
{ ca  asp a   151 } 2.531, -9.121, -7.443 
{ ca  lys a   152 } -0.867, -8.334, -8.903 
{ ca  ile a   153 } -0.103, -9.748, -12.286 
{ ca  arg a   154 } 1.062, -12.978, -10.685 
{ ca  asp a   155 } -2.378, -13.446, -9.070 
{ ca  thr a   156 } -4.252, -13.094, -12.338 
{ ca  ala a   157 } -1.607, -15.035, -14.222 
{ ca  thr a   158 } -1.725, -18.020, -11.824 
{ ca  ser a   159 } -5.538, -17.961, -11.955 
{ ca  his a   160 } -5.703, -18.445, -15.757 
{ ca  glu a   161 } -2.302, -20.172, -16.135 
{ ca  arg a   162 } -0.951, -17.473, -18.507 
{ ca  thr a   163 } 2.262, -15.807, -19.566 
{ ca  tyr a   164 } 2.811, -12.119, -18.991 
{ ca  val a   165 } 5.222, -9.769, -20.696 
{ ca  val a   165 } 5.222, -9.769, -20.696 
{ ca  glu a   167 } 7.361, -3.620, -19.887 
{ ca  val a   168 } 9.112, -1.414, -17.295 
{ ca  met a   169 } 10.892, 1.936, -17.676 
{ ca  gly a   170 } 14.671, 2.496, -17.860 
{ ca  arg a   171 } 15.393, 3.834, -21.313 
{ ca  his a   172 } 19.022, 2.680, -21.531 
{ ca  ala a   173 } 19.533, 1.022, -18.115 
{ ca  gly a   174 } 18.281, -2.418, -16.971 
{ ca  asp a   175 } 18.249, -1.722, -13.224 
{ ca  ile a   176 } 14.428, -1.507, -12.851 
{ ca  ala a   177 } 13.891, -4.659, -14.934 
{ ca  leu a   178 } 16.773, -6.520, -13.230 
{ ca  trp a   179 } 15.998, -5.830, -9.563 
{ ca  ser a   180 } 12.273, -6.089, -10.367 
{ ca  gly a   181 } 12.564, -9.367, -12.190 
{ ca  leu a   182 } 14.570, -10.762, -9.286 
{ ca  ala a   183 } 12.349, -9.492, -6.484 
{ ca  gly a   184 } 9.346, -10.617, -8.532 
{ ca  gly a   185 } 10.350, -14.228, -9.197 
{ ca  ala a   186 } 10.358, -13.495, -12.966 
{ ca  glu a   187 } 11.208, -16.275, -15.418 
{ ca  thr a   188 } 13.064, -14.038, -17.897 
{ ca  ile a   189 } 14.675, -10.560, -17.609 
{ ca  leu a   190 } 15.651, -8.934, -20.935 
{ ca  ile a   191 } 17.974, -6.020, -20.204 
{ ca  pro a   192 } 20.106, -3.902, -22.517 
{ ca  glu a   193 } 23.455, -4.674, -20.889 
{ ca  ala a   194 } 23.156, -8.462, -21.351 
{ ca  asp a   195 } 22.875, -10.647, -24.399 
{ ca  tyr a   196 } 19.815, -12.850, -24.597 
{ ca  asp a   197 } 19.082, -15.565, -27.155 
{ ca  met a   198 } 15.379, -15.796, -27.907 
{ ca  asn a   199 } 15.660, -19.522, -28.595 
{ ca  asp a   200 } 17.197, -20.141, -25.144 
{ ca  val a   201 } 14.613, -17.873, -23.585 
{ ca  ile a   202 } 11.946, -20.025, -25.222 
{ ca  ala a   203 } 13.563, -23.292, -24.247 
{ ca  arg a   204 } 13.783, -22.126, -20.654 
{ ca  leu a   205 } 10.042, -21.113, -20.592 
{ ca  lys a   206 } 8.872, -24.389, -22.115 
{ ca  arg a   207 } 10.998, -26.350, -19.638 
{ ca  gly a   208 } 9.456, -24.478, -16.657 
{ ca  his a   209 } 5.955, -25.046, -17.970 
{ ca  glu a   210 } 6.582, -28.782, -18.325 
{ ca  arg a   211 } 8.035, -28.874, -14.820 
{ ca  gly a   212 } 4.551, -27.679, -13.749 
{ ca  lys a   213 } 4.686, -23.891, -13.341 
{ ca  lys a   214 } 1.230, -22.326, -13.534 
{ ca  his a   215 } 2.125, -18.806, -14.720 
{ ca  ser a   216 } 5.170, -17.286, -16.440 
{ ca  ile a   217 } 6.405, -13.643, -15.925 
{ ca  ile a   218 } 8.691, -11.861, -18.416 
{ ca  ile a   219 } 10.244, -8.413, -17.633 
{ ca  val a   220 } 11.476, -6.368, -20.568 
{ ca  ala a   221 } 13.311, -3.049, -20.036 
{ ca  glu a   222 } 11.830, -0.526, -22.514 
{ ca  gly a   223 } 15.476, -0.169, -23.577 
{ ca  val a   224 } 15.436, -3.586, -25.292 
{ ca  gly a   225 } 12.288, -3.153, -27.369 
{ ca  ser a   226 } 8.516, -3.434, -26.998 
{ ca  gly a   227 } 6.508, -5.574, -24.614 
{ ca  val a   228 } 4.206, -6.165, -27.562 
{ ca  asp a   229 } 6.907, -7.562, -29.834 
{ ca  phe a   230 } 8.210, -9.980, -27.192 
{ ca  gly a   231 } 4.613, -11.011, -26.347 
{ ca  arg a   232 } 3.994, -11.731, -30.042 
{ ca  gln a   233 } 7.283, -13.591, -30.457 
{ ca  ile a   234 } 6.835, -15.697, -27.315 
{ ca  gln a   235 } 3.178, -16.587, -27.973 
{ ca  glu a   236 } 4.009, -17.659, -31.525 
{ ca  ala a   237 } 6.773, -19.968, -30.317 
{ ca  thr a   238 } 5.157, -21.551, -27.252 
{ ca  gly a   239 } 1.451, -21.472, -28.038 
{ ca  phe a   240 } 0.923, -20.249, -24.439 
{ ca  glu a   241 } -1.804, -17.697, -23.776 
{ ca  thr a   242 } 0.077, -14.461, -23.469 
{ ca  arg a   243 } -0.735, -10.941, -22.283 
{ ca  val a   244 } 1.381, -7.732, -22.248 
{ ca  thr a   245 } 1.354, -4.928, -19.646 
{ ca  val a   246 } 3.125, -1.575, -20.106 
{ ca  leu a   247 } 3.416, -0.099, -16.615 
{ ca  gly a   248 } 4.332, 3.333, -17.979 
{ ca  his a   249 } 4.366, 6.474, -15.874 
{ ca  val a   250 } 2.876, 4.979, -12.652 
{ ca  gln a   251 } 6.614, 4.107, -12.254 
{ ca  arg a   252 } 7.308, 7.847, -11.914 
{ ca  gly a   254 } 4.018, 7.900, -5.548 
{ ca  ser a   255 } 5.095, 8.651, -2.017 
{ ca  pro a   256 } 8.815, 7.829, -1.858 
{ ca  thr a   257 } 10.064, 4.809, 0.067 
{ ca  ala a   258 } 12.014, 5.006, 3.327 
{ ca  phe a   259 } 15.374, 4.498, 1.508 
{ ca  asp a   260 } 14.504, 7.111, -1.107 
{ ca  arg a   261 } 13.556, 9.475, 1.689 
{ ca  val a   262 } 16.825, 8.877, 3.619 
{ ca  leu a   263 } 18.954, 8.862, 0.479 
{ ca  ala a   264 } 17.473, 12.194, -0.781 
{ ca  ser a   265 } 17.867, 13.771, 2.656 
{ ca  arg a   266 } 21.523, 12.832, 3.061 
{ ca  leu a   267 } 22.473, 13.787, -0.500 
{ ca  gly a   268 } 20.565, 17.095, -0.587 
{ ca  ala a   269 } 22.335, 18.044, 2.688 
{ ca  arg a   270 } 25.746, 16.985, 1.482 
{ ca  ala a   271 } 25.233, 19.088, -1.644 
{ ca  val a   272 } 24.883, 22.148, 0.598 
{ ca  glu a   273 } 27.957, 21.259, 2.656 
{ ca  leu a   274 } 29.889, 21.360, -0.646 
{ ca  leu a   275 } 28.611, 24.774, -1.857 
{ ca  leu a   276 } 29.538, 26.295, 1.553 
{ ca  glu a   277 } 33.011, 24.668, 1.584 
{ ca  gly a   278 } 33.442, 26.310, -1.826 
{ ca  lys a   279 } 33.599, 23.024, -3.779 
{ ca  gly a   280 } 31.646, 23.446, -7.031 
{ ca  gly a   281 } 31.282, 21.622, -10.292 
{ ca  arg a   282 } 30.873, 18.389, -8.238 
{ ca  cys a   283 } 28.035, 15.786, -8.204 
{ ca  val a   284 } 27.071, 13.749, -5.052 
{ ca  gly a   285 } 26.185, 10.063, -4.704 
{ ca  ile a   286 } 26.430, 6.923, -2.634 
{ ca  gln a   287 } 29.220, 4.446, -3.371 
{ ca  asn a   288 } 29.983, 1.253, -1.507 
{ ca  asn a   289 } 27.493, 2.512, 0.985 
{ ca  gln a   290 } 29.242, 5.834, 1.653 
{ ca  leu a   291 } 28.630, 9.373, 0.582 
{ ca  val a   292 } 31.066, 10.325, -2.151 
{ ca  asp a   293 } 31.336, 13.297, -4.504 
{ ca  his a   294 } 32.983, 13.548, -7.918 
{ ca  asp a   295 } 33.820, 16.148, -10.535 
{ ca  ile a   296 } 30.807, 16.346, -12.827 
{ ca  ala a   297 } 33.097, 16.331, -15.893 
{ ca  glu a   298 } 34.752, 13.112, -14.828 
{ ca  ala a   299 } 31.707, 11.304, -13.437 
{ ca  leu a   300 } 30.091, 11.993, -16.829 
{ ca  ala a   301 } 33.045, 10.366, -18.640 
{ ca  asn a   303 } 32.369, 7.168, -16.687 
{ ca  lys a   304 } 29.733, 4.592, -17.795 
{ ca  his a   305 } 26.722, 2.923, -16.177 
{ ca  thr a   306 } 26.868, -0.814, -15.693 
{ ca  ile a   307 } 24.908, -3.407, -13.702 
{ ca  asp a   308 } 25.924, -6.348, -11.519 
{ ca  gln a   309 } 26.211, -9.058, -14.233 
{ ca  arg a   310 } 26.343, -11.656, -11.377 
{ ca  met a   311 } 22.847, -10.503, -10.248 
{ ca  tyr a   312 } 21.651, -11.255, -13.779 
{ ca  ala a   313 } 23.071, -14.813, -13.504 
{ ca  leu a   314 } 21.441, -15.250, -10.130 
{ ca  ser a   315 } 18.223, -14.275, -11.955 
{ ca  lys a   316 } 18.728, -17.234, -14.295 
{ ca  glu a   317 } 19.570, -19.718, -11.484 
{ ca  leu a   318 } 16.670, -19.006, -9.119 
{ ca  ser a   319 } 14.178, -19.078, -12.020 
{ ca  ile a   320 } 14.331, -22.867, -12.412 
@subgroup {Subunit 2}    dominant
@vectorlist {ca-ca} color= pink     
{ ca  met b   1 } P -28.049, -32.245, 0.597 
{ ca  met b   1 } -28.049, -32.245, 0.597 
{ ca  lys b   2 } -24.804, -33.691, 1.903 
{ ca  arg b   3 } -22.400, -32.832, -0.881 
{ ca  ile b   4 } -21.914, -29.621, -2.901 
{ ca  gly b   5 } -19.386, -28.380, -5.423 
{ ca  val b   6 } -17.842, -24.904, -5.901 
{ ca  leu b   7 } -16.018, -23.467, -8.906 
{ ca  thr b   8 } -14.712, -20.197, -10.320 
{ ca  ser b   9 } -15.347, -19.178, -13.961 
{ ca  gly b   10 } -14.841, -16.122, -16.148 
{ ca  gly b   11 } -12.047, -13.660, -15.452 
{ ca  asp b   12 } -10.613, -13.933, -11.942 
{ ca  ser b   13 } -11.243, -11.205, -9.415 
{ ca  pro b   14 } -9.778, -10.231, -6.043 
{ ca  gly b   15 } -12.090, -11.777, -3.430 
{ ca  met b   16 } -12.824, -15.070, -5.221 
{ ca  asn b   17 } -10.846, -16.878, -2.481 
{ ca  ala b   18 } -12.791, -15.242, 0.393 
{ ca  ala b   19 } -15.889, -16.481, -1.473 
{ ca  ile b   20 } -14.568, -20.037, -1.822 
{ ca  arg b   21 } -13.657, -19.847, 1.845 
{ ca  ser b   22 } -17.264, -18.935, 3.052 
{ ca  val b   23 } -18.861, -21.555, 0.790 
{ ca  val b   24 } -16.438, -24.110, 2.253 
{ ca  arg b   25 } -16.868, -22.998, 5.860 
{ ca  lys b   26 } -20.567, -22.269, 5.564 
{ ca  ala b   27 } -21.222, -25.792, 4.133 
{ ca  ile b   28 } -19.142, -27.637, 6.794 
{ ca  tyr b   29 } -20.715, -25.715, 9.699 
{ ca  his b   30 } -23.862, -27.216, 8.328 
{ ca  gly b   31 } -22.256, -30.653, 8.160 
{ ca  val b   32 } -22.359, -30.673, 4.352 
{ ca  glu b   33 } -19.188, -31.678, 2.483 
{ ca  val b   34 } -17.572, -29.468, -0.168 
{ ca  tyr b   35 } -15.895, -30.473, -3.402 
{ ca  gly b   36 } -13.799, -28.147, -5.503 
{ ca  val b   37 } -13.929, -28.070, -9.295 
{ ca  tyr b   38 } -10.578, -26.884, -10.753 
{ ca  his b   39 } -10.417, -24.970, -14.028 
{ ca  gly b   40 } -14.079, -23.970, -13.910 
{ ca  tyr b   41 } -16.404, -25.565, -16.472 
{ ca  ala b   42 } -13.479, -27.081, -18.344 
{ ca  gly b   43 } -12.393, -29.191, -15.373 
{ ca  leu b   44 } -16.002, -29.941, -14.576 
{ ca  ile b   45 } -16.118, -31.968, -17.775 
{ ca  ala b   46 } -12.594, -33.384, -17.297 
{ ca  gly b   47 } -13.609, -34.397, -13.753 
{ ca  asn b   48 } -10.908, -32.254, -12.053 
{ ca  ile b   49 } -12.693, -32.478, -8.651 
{ ca  lys b   50 } -11.072, -32.660, -5.149 
{ ca  lys b   51 } -12.679, -32.576, -1.672 
{ ca  leu b   52 } -11.999, -29.329, 0.271 
{ ca  glu b   53 } -11.324, -29.740, 3.994 
{ ca  val b   54 } -11.830, -26.993, 6.583 
{ ca  gly b   55 } -8.107, -26.005, 6.473 
{ ca  asp b   56 } -7.579, -26.008, 2.723 
{ ca  val b   57 } -9.255, -22.571, 2.808 
{ ca  gly b   58 } -7.149, -21.404, 5.712 
{ ca  asp b   59 } -4.821, -18.601, 4.628 
{ ca  ile b   60 } -6.501, -17.614, 1.402 
{ ca  ile b   61 } -8.773, -14.847, 2.734 
{ ca  his b   62 } -6.290, -12.054, 1.933 
{ ca  arg b   63 } -5.225, -13.459, -1.460 
{ ca  gly b   64 } -6.041, -12.440, -5.018 
{ ca  gly b   65 } -6.913, -14.793, -7.904 
{ ca  thr b   66 } -8.636, -18.109, -7.295 
{ ca  ile b   67 } -7.041, -21.184, -5.782 
{ ca  leu b   68 } -9.425, -23.358, -7.921 
{ ca  tyr b   69 } -8.196, -21.713, -11.147 
{ ca  thr b   70 } -10.481, -20.729, -14.002 
{ ca  ala b   71 } -10.768, -21.640, -17.657 
{ ca  arg b   72 } -12.811, -20.938, -20.763 
{ ca  cys b   73 } -14.997, -23.961, -21.734 
{ ca  pro b   74 } -16.722, -23.473, -25.101 
{ ca  glu b   75 } -17.599, -27.216, -24.978 
{ ca  phe b   76 } -20.006, -26.512, -22.051 
{ ca  lys b   77 } -22.303, -24.583, -24.395 
{ ca  thr b   78 } -22.949, -27.978, -25.975 
{ ca  glu b   79 } -25.557, -30.475, -24.821 
{ ca  glu b   80 } -22.899, -33.188, -24.728 
{ ca  gly b   81 } -20.746, -30.819, -22.656 
{ ca  gln b   82 } -23.453, -30.195, -20.093 
{ ca  lys b   83 } -24.026, -33.979, -20.174 
{ ca  lys b   84 } -20.415, -34.952, -19.582 
{ ca  gly b   85 } -20.130, -32.400, -16.781 
{ ca  ile b   86 } -23.446, -33.502, -15.290 
{ ca  glu b   87 } -22.093, -37.068, -15.308 
{ ca  gln b   88 } -18.958, -35.989, -13.501 
{ ca  leu b   89 } -20.986, -34.438, -10.737 
{ ca  lys b   90 } -22.977, -37.634, -10.212 
{ ca  lys b   91 } -19.908, -39.815, -10.484 
{ ca  his b   92 } -18.680, -37.819, -7.454 
{ ca  gly b   93 } -22.061, -37.692, -5.716 
{ ca  ile b   94 } -22.213, -33.911, -5.859 
{ ca  gln b   95 } -25.753, -32.595, -5.596 
{ ca  gly b   96 } -25.402, -28.779, -5.694 
{ ca  leu b   97 } -23.105, -26.241, -7.228 
{ ca  val b   98 } -21.929, -22.823, -6.138 
{ ca  val b   99 } -20.595, -21.016, -9.176 
{ ca  ile b   100 } -18.597, -17.785, -8.499 
{ ca  gly b   101 } -18.106, -15.763, -11.728 
{ ca  gly b   102 } -19.320, -13.578, -14.568 
{ ca  asp b   103 } -22.531, -13.469, -16.573 
{ ca  gly b   104 } -21.085, -15.936, -19.094 
{ ca  ser b   105 } -20.699, -18.193, -16.107 
{ ca  tyr b   106 } -24.233, -17.603, -14.760 
{ ca  gln b   107 } -25.530, -18.873, -18.122 
{ ca  gly b   108 } -23.984, -22.286, -17.399 
{ ca  ala b   109 } -25.397, -22.353, -13.874 
{ ca  lys b   110 } -28.775, -21.860, -15.582 
{ ca  lys b   111 } -28.267, -24.697, -18.133 
{ ca  leu b   112 } -27.320, -26.880, -15.173 
{ ca  thr b   113 } -30.529, -26.322, -13.141 
{ ca  glu b   114 } -32.636, -26.915, -16.294 
{ ca  his b   115 } -30.975, -30.387, -16.390 
{ ca  gly b   116 } -31.619, -30.827, -12.652 
{ ca  phe b   117 } -28.534, -29.515, -10.879 
{ ca  pro b   118 } -29.269, -26.895, -8.270 
{ ca  cys b   119 } -26.806, -23.997, -8.753 
{ ca  val b   120 } -26.356, -20.536, -7.280 
{ ca  gly b   121 } -24.208, -17.873, -8.955 
{ ca  val b   122 } -21.915, -15.570, -6.908 
{ ca  pro b   123 } -20.971, -12.373, -8.786 
{ ca  gly b   124 } -17.206, -12.362, -9.292 
{ ca  thr b   125 } -15.902, -9.775, -11.786 
{ ca  ile b   126 } -13.952, -6.511, -11.365 
{ ca  asp b   127 } -16.298, -4.893, -13.896 
{ ca  asn b   128 } -19.396, -4.545, -11.674 
{ ca  asp b   129 } -21.520, -5.508, -14.724 
{ ca  ile b   130 } -23.677, -8.241, -13.235 
{ ca  pro b   131 } -27.431, -7.822, -12.813 
{ ca  gly b   132 } -29.004, -8.570, -9.456 
{ ca  thr b   133 } -26.154, -6.858, -7.568 
{ ca  asp b   134 } -25.103, -3.224, -6.903 
{ ca  phe b   135 } -21.628, -4.478, -6.339 
{ ca  thr b   136 } -19.728, -7.511, -7.693 
{ ca  ile b   137 } -16.845, -9.195, -5.782 
{ ca  gly b   138 } -13.529, -7.587, -6.812 
{ ca  phe b   139 } -14.850, -4.196, -7.995 
{ ca  asp b   140 } -13.808, -2.355, -4.778 
{ ca  thr b   141 } -10.370, -3.867, -5.160 
{ ca  ala b   142 } -9.958, -2.794, -8.830 
{ ca  leu b   143 } -11.095, 0.685, -7.896 
{ ca  asn b   144 } -8.483, 1.067, -5.248 
{ ca  thr b   145 } -5.914, -0.367, -7.714 
{ ca  val b   146 } -6.832, 2.297, -10.238 
{ ca  ile b   147 } -6.850, 5.352, -7.883 
{ ca  asp b   148 } -3.517, 4.053, -6.679 
{ ca  ala b   149 } -2.412, 4.539, -10.288 
{ ca  ile b   150 } -4.102, 7.918, -10.677 
{ ca  asp b   151 } -2.531, 9.121, -7.443 
{ ca  lys b   152 } 0.867, 8.334, -8.903 
{ ca  ile b   153 } 0.103, 9.748, -12.286 
{ ca  arg b   154 } -1.062, 12.978, -10.685 
{ ca  asp b   155 } 2.378, 13.446, -9.070 
{ ca  thr b   156 } 4.252, 13.094, -12.338 
{ ca  ala b   157 } 1.607, 15.035, -14.222 
{ ca  thr b   158 } 1.725, 18.020, -11.824 
{ ca  ser b   159 } 5.538, 17.961, -11.955 
{ ca  his b   160 } 5.703, 18.445, -15.757 
{ ca  glu b   161 } 2.302, 20.172, -16.135 
{ ca  arg b   162 } 0.951, 17.473, -18.507 
{ ca  thr b   163 } -2.262, 15.807, -19.566 
{ ca  tyr b   164 } -2.811, 12.119, -18.991 
{ ca  val b   165 } -5.222, 9.769, -20.696 
{ ca  ile b   166 } -5.648, 6.702, -18.474 
{ ca  glu b   167 } -7.361, 3.620, -19.887 
{ ca  val b   168 } -9.112, 1.414, -17.295 
{ ca  met b   169 } -10.892, -1.936, -17.676 
{ ca  gly b   170 } -14.671, -2.496, -17.860 
{ ca  arg b   171 } -15.393, -3.834, -21.313 
{ ca  his b   172 } -19.022, -2.680, -21.531 
{ ca  ala b   173 } -19.533, -1.022, -18.115 
{ ca  gly b   174 } -18.281, 2.418, -16.971 
{ ca  asp b   175 } -18.249, 1.722, -13.224 
{ ca  ile b   176 } -14.428, 1.507, -12.851 
{ ca  ala b   177 } -13.891, 4.659, -14.934 
{ ca  leu b   178 } -16.773, 6.520, -13.230 
{ ca  trp b   179 } -15.998, 5.830, -9.563 
{ ca  ser b   180 } -12.273, 6.089, -10.367 
{ ca  gly b   181 } -12.564, 9.367, -12.190 
{ ca  leu b   182 } -14.570, 10.762, -9.286 
{ ca  ala b   183 } -12.349, 9.492, -6.484 
{ ca  gly b   184 } -9.346, 10.617, -8.532 
{ ca  gly b   185 } -10.350, 14.228, -9.197 
{ ca  ala b   186 } -10.358, 13.495, -12.966 
{ ca  glu b   187 } -11.208, 16.275, -15.418 
{ ca  thr b   188 } -13.064, 14.038, -17.897 
{ ca  ile b   189 } -14.675, 10.560, -17.609 
{ ca  leu b   190 } -15.651, 8.934, -20.935 
{ ca  ile b   191 } -17.974, 6.020, -20.204 
{ ca  pro b   192 } -20.106, 3.902, -22.517 
{ ca  glu b   193 } -23.455, 4.674, -20.889 
{ ca  ala b   194 } -23.156, 8.462, -21.351 
{ ca  asp b   195 } -22.875, 10.647, -24.399 
{ ca  tyr b   196 } -19.815, 12.850, -24.597 
{ ca  asp b   197 } -19.082, 15.565, -27.155 
{ ca  met b   198 } -15.379, 15.796, -27.907 
{ ca  asn b   199 } -15.660, 19.522, -28.595 
{ ca  asp b   200 } -17.197, 20.141, -25.144 
{ ca  val b   201 } -14.613, 17.873, -23.585 
{ ca  ile b   202 } -11.946, 20.025, -25.222 
{ ca  ala b   203 } -13.563, 23.292, -24.247 
{ ca  arg b   204 } -13.783, 22.126, -20.654 
{ ca  leu b   205 } -10.042, 21.113, -20.592 
{ ca  lys b   206 } -8.872, 24.389, -22.115 
{ ca  arg b   207 } -10.998, 26.350, -19.638 
{ ca  gly b   208 } -9.456, 24.478, -16.657 
{ ca  his b   209 } -5.955, 25.046, -17.970 
{ ca  glu b   210 } -6.582, 28.782, -18.325 
{ ca  arg b   211 } -8.035, 28.874, -14.820 
{ ca  gly b   212 } -4.551, 27.679, -13.749 
{ ca  lys b   213 } -4.686, 23.891, -13.341 
{ ca  lys b   214 } -1.230, 22.326, -13.534 
{ ca  his b   215 } -2.125, 18.806, -14.720 
{ ca  ser b   216 } -5.170, 17.286, -16.440 
{ ca  ile b    217 } -6.405, 13.643, -15.925
{ ca  ile b   218 } -8.691, 11.861, -18.416 
{ ca  ile b   219 } -10.244, 8.413, -17.633 
{ ca  val b   220 } -11.476, 6.368, -20.568 
{ ca  ala b   221 } -13.311, 3.049, -20.036 
{ ca  glu b   222 } -11.830, 0.526, -22.514 
{ ca  gly b   223 } -15.476, 0.169, -23.577 
{ ca  val b   224 } -15.436, 3.586, -25.292 
{ ca  gly b   225 } -12.288, 3.153, -27.369 
{ ca  ser b   226 } -8.516, 3.434, -26.998 
{ ca  gly b   227 } -6.508, 5.574, -24.614 
{ ca  val b   228 } -4.206, 6.165, -27.562 
{ ca  asp b   229 } -6.907, 7.562, -29.834 
{ ca  phe b   230 } -8.210, 9.980, -27.192 
{ ca  gly b   231 } -4.613, 11.011, -26.347 
{ ca  arg b   232 } -3.994, 11.731, -30.042 
{ ca  gln b   233 } -7.283, 13.591, -30.457 
{ ca  ile b   234 } -6.835, 15.697, -27.315 
{ ca  gln b   235 } -3.178, 16.587, -27.973 
{ ca  glu b   236 } -4.009, 17.659, -31.525 
{ ca  ala b   237 } -6.773, 19.968, -30.317 
{ ca  thr b   238 } -5.157, 21.551, -27.252 
{ ca  gly b   239 } -1.451, 21.472, -28.038 
{ ca  phe b   240 } -0.923, 20.249, -24.439 
{ ca  glu b   241 } 1.804, 17.697, -23.776 
{ ca  thr b   242 } -0.077, 14.461, -23.469 
{ ca  arg b   243 } 0.735, 10.941, -22.283 
{ ca  val b   244 } -1.381, 7.732, -22.248 
{ ca  thr b   245 } -1.354, 4.928, -19.646 
{ ca  val b   246 } -3.125, 1.575, -20.106 
{ ca  leu b   247 } -3.416, 0.099, -16.615 
{ ca  gly b   248 } -4.332, -3.333, -17.979 
{ ca  his b   249 } -4.366, -6.474, -15.874 
{ ca  val b   250 } -2.876, -4.979, -12.652 
{ ca  gln b   251 } -6.614, -4.107, -12.254 
{ ca  arg b   252 } -7.308, -7.847, -11.914 
{ ca  gly b   253 } -4.980, -8.900, -9.062 
{ ca  gly b   254 } -4.018, -7.900, -5.548 
{ ca  ser b   255 } -5.095, -8.651, -2.017 
{ ca  pro b   256 } -8.815, -7.829, -1.858 
{ ca  thr b   257 } -10.064, -4.809, 0.067 
{ ca  ala b   258 } -12.014, -5.006, 3.327 
{ ca  phe b   259 } -15.374, -4.498, 1.508 
{ ca  asp b   260 } -14.504, -7.111, -1.107 
{ ca  arg b   261 } -13.556, -9.475, 1.689 
{ ca  val b   262 } -16.825, -8.877, 3.619 
{ ca  leu b   263 } -18.954, -8.862, 0.479 
{ ca  ala b   264 } -17.473, -12.194, -0.781 
{ ca  ser b   265 } -17.867, -13.771, 2.656 
{ ca  arg b   266 } -21.523, -12.832, 3.061 
{ ca  leu b   267 } -22.473, -13.787, -0.500 
{ ca  gly b   268 } -20.565, -17.095, -0.587 
{ ca  ala b   269 } -22.335, -18.044, 2.688 
{ ca  arg b   270 } -25.746, -16.985, 1.482 
{ ca  ala b   271 } -25.233, -19.088, -1.644 
{ ca  val b   272 } -24.883, -22.148, 0.598 
{ ca  glu b   273 } -27.957, -21.259, 2.656 
{ ca  leu b   274 } -29.889, -21.360, -0.646 
{ ca  leu b   275 } -28.611, -24.774, -1.857 
{ ca  leu b   276 } -29.538, -26.295, 1.553 
{ ca  glu b   277 } -33.011, -24.668, 1.584 
{ ca  gly b   278 } -33.442, -26.310, -1.826 
{ ca  lys b   279 } -33.599, -23.024, -3.779 
{ ca  gly b   280 } -31.646, -23.446, -7.031 
{ ca  gly b   281 } -31.282, -21.622, -10.292 
{ ca  arg b   282 } -30.873, -18.389, -8.238 
{ ca  cys b   283 } -28.035, -15.786, -8.204 
{ ca  val b   284 } -27.071, -13.749, -5.052 
{ ca  gly b   285 } -26.185, -10.063, -4.704 
{ ca  ile b   286 } -26.430, -6.923, -2.634 
{ ca  gln b   287 } -29.220, -4.446, -3.371 
{ ca  asn b   288 } -29.983, -1.253, -1.507 
{ ca  asn b   289 } -27.493, -2.512, 0.985 
{ ca  gln b   290 } -29.242, -5.834, 1.653 
{ ca  leu b   291 } -28.630, -9.373, 0.582 
{ ca  val b   292 } -31.066, -10.325, -2.151 
{ ca  asp b   293 } -31.336, -13.297, -4.504 
{ ca  his b   294 } -32.983, -13.548, -7.918 
{ ca  asp b   295 } -33.820, -16.148, -10.535 
{ ca  ile b   296 } -30.807, -16.346, -12.827 
{ ca  ala b   297 } -33.097, -16.331, -15.893 
{ ca  glu b   298 } -34.752, -13.112, -14.828 
{ ca  ala b   299 } -31.707, -11.304, -13.437 
{ ca  leu b   300 } -30.091, -11.993, -16.829 
{ ca  ala b   301 } -33.045, -10.366, -18.640 
{ ca  asn b   303 } -32.369, -7.168, -16.687 
{ ca  lys b   304 } -29.733, -4.592, -17.795 
{ ca  his b   305 } -26.722, -2.923, -16.177 
{ ca  thr b   306 } -26.868, 0.814, -15.693 
{ ca  ile b   307 } -24.908, 3.407, -13.702 
{ ca  asp b   308 } -25.924, 6.348, -11.519 
{ ca  gln b   309 } -26.211, 9.058, -14.233 
{ ca  arg b   310 } -26.343, 11.656, -11.377 
{ ca  met b   311 } -22.847, 10.503, -10.248 
{ ca  tyr b   312 } -21.651, 11.255, -13.779 
{ ca  ala b   313 } -23.071, 14.813, -13.504 
{ ca  leu b   314 } -21.441, 15.250, -10.130 
{ ca  ser b   315 } -18.223, 14.275, -11.955 
{ ca  lys b   316 } -18.728, 17.234, -14.295 
{ ca  glu b   317 } -19.570, 19.718, -11.484 
{ ca  leu b   318 } -16.670, 19.006, -9.119 
{ ca  ser b   319 } -14.178, 19.078, -12.020 
{ ca  ile b   320 } -14.331, 22.867, -12.412 
@subgroup {Glu 161} dominant    
@vectorlist {sc} color= red     
{ ca  glu b   161 } P 2.302, 20.172, -16.135 
{ cb  glu b   161 } 2.479, 21.651, -16.380 
{ cg  glu b   161 } 3.029, 22.541, -15.274 
{ cd  glu b   161 } 2.998, 24.021, -15.560 
{ oe1 glu b   161 } 2.991, 24.866, -14.681 
{ cd  glu b   161 } P 2.998, 24.021, -15.560 
{ oe2 glu b   161 } 2.973, 24.368, -16.763 
{ ca  glu  a  161 } P -2.302, -20.172, -16.135 
{ cb  glu a   161 } -2.479, -21.651, -16.380 
{ cg  glu a   161 } -3.029, -22.541, -15.274 
{ cd  glu a    161 } -2.998, -24.021, -15.560 
{ oe1 glu a   161 } -2.991, -24.866, -14.681 
{ cd  glu a   161 } P -2.998, -24.021, -15.560 
{ oe2 glu a   161 } -2.973, -24.368, -16.763 
@subgroup {Arg 162} dominant    
@vectorlist {sc} color= cyan     
{ ca  arg a   162 } P -0.951, -17.473, -18.507 
{ cb  arg a   162 } -1.826, -16.200, -18.687 
{ cg  arg a   162 } -3.303, -16.367, -18.924 
{ cd  arg a   162 } -4.016, -15.074, -19.015 
{ ne  arg a   162 } -5.308, -15.274, -19.679 
{ cz  arg a   162 } -6.316, -14.398, -19.664 
{ nh1 arg a   162 } -6.293, -13.262, -18.963 
{ cz  arg a   162 } P -6.316, -14.398, -19.664 
{ nh2 arg a   162 } -7.354, -14.608, -20.464 
{ ca  arg b   162 } P 0.951, 17.473, -18.507 
{ cb  arg b   162 } 1.826, 16.200, -18.687 
{ cg  arg b   162 } 3.303, 16.367, -18.924 
{ cd  arg b   162 } 4.016, 15.074, -19.015 
{ ne  arg b   162 } 5.308, 15.274, -19.679 
{ cz  arg b   162 } 6.316, 14.398, -19.664 
{ nh1 arg b   162 } 6.293, 13.262, -18.963 
{ cz  arg b   162 } P 6.316, 14.398, -19.664 
{ nh2 arg b   162 } 7.354, 14.608, -20.464 
@vectorlist {H bonds} color= white     
{ o63   F6P 1 } P U 7.922, 11.759, -19.333 
{ nh1 arg b   162 } L U 6.800, 12.794, -19.078 
{ nh2 arg b   162 } P U 7.733, 14.079, -20.721 
{ o61   F6P 1 } L U 8.628, 12.832, -21.329 
{ o61  F6P 2 } P U -8.628, -12.832, -21.329 
{ nh2 arg a   162 } L U -7.733, -14.079, -20.721 
{ nh1 arg a   162 } P U -6.800, -12.794, -19.078 
{ o63  F6P 2 } L U -7.922, -11.759, -19.333 
@subgroup {F6P} dominant    
@vectorlist {ht} color= hotpink     
{ o1   F6P 2 } P -13.626, -9.201, -16.841 
{ o1   F6P 2 } -13.626, -9.201, -16.841 
{ c1   F6P 2 } -12.362, -8.703, -16.439 
{ c2   F6P 2 } -11.618, -8.043, -17.622 
{ o2   F6P 2 } -10.391, -7.537, -17.045 
{ c2   F6P 2 } P -11.618, -8.043, -17.622 
{ c3   F6P 2 } -12.298, -6.949, -18.475 
{ o3   F6P 2 } -12.364, -5.618, -17.960 
{ c3   F6P 2 } P -12.298, -6.949, -18.475 
{ c4   F6P 2 } -11.382, -6.919, -19.704 
{ o4   F6P 2 } -11.951, -6.378, -20.896 
{ c4   F6P 2 } P -11.382, -6.919, -19.704 
{ c5   F6P 2 } -11.131, -8.424, -19.887 
{ c2   F6P 2 } P -11.618, -8.043, -17.622 
{ o5   F6P 2 } -11.283, -9.091, -18.590 
{ c5   F6P 2 } P -11.131, -8.424, -19.887 
{ c6   F6P 2 } -9.752, -8.729, -20.473 
{ o6   F6P 2 } -9.864, -10.053, -21.018 
{ p6   F6P 2 } -8.610, -11.065, -20.899 
{ o61  F6P 2 } -9.007, -12.303, -21.586 
{ p6   F6P 2 } P -8.610, -11.065, -20.899 
{ o62  F6P 2 } -7.448, -10.426, -21.553 
{ p6   F6P 2 } P -8.610, -11.065, -20.899 
{ o63  F6P 2 } -8.429, -11.291, -19.448 
{ c5   F6P 2 } P -11.131, -8.424, -19.887 
{ o5   F6P 2 } -11.283, -9.091, -18.590 
{ oh1   F6P 1 } P 13.626, 9.201, -16.841 
{ o1   F6P 1 } 13.626, 9.201, -16.841 
{ c1   F6P 1 } 12.362, 8.703, -16.439 
{ c2   F6P 1 } 11.618, 8.043, -17.622 
{ o2   F6P 1 } 10.391, 7.537, -17.045 
{ c2   F6P 1 } P 11.618, 8.043, -17.622 
{ c3   F6P 1 } 12.298, 6.949, -18.475 
{ o3   F6P 1 } 12.364, 5.618, -17.960 
{ c3   F6P 1 } P 12.298, 6.949, -18.475 
{ c4   F6P 1 } 11.382, 6.919, -19.704 
{ o4   F6P 1 } 11.951, 6.378, -20.896 
{ c4   F6P 1 } P 11.382, 6.919, -19.704 
{ c5   F6P 1 } 11.131, 8.424, -19.887 
{ c2   F6P 1 } P 11.618, 8.043, -17.622 
{ o5   F6P 1 } 11.283, 9.091, -18.590 
{ c5   F6P 1 } P 11.131, 8.424, -19.887 
{ c6   F6P 1 } 9.752, 8.729, -20.473 
{ o6   F6P 1 } 9.864, 10.053, -21.018 
{ p6   F6P 1 } 8.610, 11.065, -20.899 
{ o61   F6P 1 } 9.007, 12.303, -21.586 
{ p6   F6P 1 } P 8.610, 11.065, -20.899 
{ o62   F6P 1 } 7.448, 10.426, -21.553 
{ p6   F6P 1 } P 8.610, 11.065, -20.899 
{ o63   F6P 1 } 8.429, 11.291, -19.448 
{ c5   F6P 1 } P 11.131, 8.424, -19.887 
{ o5   F6P 1 } 11.283, 9.091, -18.590 
@subgroup {ADP-active} dominant    
@vectorlist {ht} color= green     
{ pb  ADP-active 2} P -17.207, -14.327, -19.200 
{ pb  ADP-active 2} -17.207, -14.327, -19.200 
{ o1b ADP-active 2} -18.467, -13.879, -19.870 
{ pb  ADP-active 2} P -17.207, -14.327, -19.200 
{ o2b ADP-active 2} -16.005, -14.060, -20.016 
{ pb  ADP-active 2} P -17.207, -14.327, -19.200 
{ o3b ADP-active 2} -17.257, -13.902, -17.775 
{ pa  ADP-active 2} P -17.492, -16.818, -20.503 
{ o1a ADP-active 2} -18.465, -16.093, -21.392 
{ pa  ADP-active 2} P -17.492, -16.818, -20.503 
{ o2a ADP-active 2} -16.225, -16.929, -21.270 
{ pb  ADP-active 2} P -17.207, -14.327, -19.200 
{ o3a ADP-active 2} -17.237, -15.958, -19.130 
{ pa  ADP-active 2} P -17.492, -16.818, -20.503 
{ o5* ADP-active 2} -18.125, -18.116, -19.886 
{ c5* ADP-active 2} -17.427, -18.813, -18.814 
{ c4* ADP-active 2} -17.740, -20.254, -19.260 
{ o4* ADP-active 2} -19.179, -20.339, -18.894 
{ c4* ADP-active 2} P -17.740, -20.254, -19.260 
{ c3* ADP-active 2} -17.857, -20.521, -20.760 
{ o3* ADP-active 2} -16.671, -20.680, -21.514 
{ c3* ADP-active 2} P -17.857, -20.521, -20.760 
{ c2* ADP-active 2} -18.731, -21.770, -20.744 
{ o2* ADP-active 2} -18.312, -23.057, -20.306 
{ o4* ADP-active 2} P -19.179, -20.339, -18.894 
{ c1* ADP-active 2} -19.824, -21.257, -19.736 
{ n9  ADP-active 2} -20.906, -20.730, -20.563 
{ c8  ADP-active 2} -21.078, -19.570, -21.251 
{ n7  ADP-active 2} -22.222, -19.602, -21.942 
{ c5  ADP-active 2} -22.766, -20.851, -21.708 
{ c6  ADP-active 2} -23.937, -21.502, -22.134 
{ n6  ADP-active 2} -24.828, -20.917, -22.933 
{ c6  ADP-active 2} P -23.937, -21.502, -22.134 
{ n1  ADP-active 2} -24.161, -22.776, -21.688 
{ c2  ADP-active 2} -23.220, -23.324, -20.855 
{ n3  ADP-active 2} -22.106, -22.823, -20.394 
{ n9  ADP-active 2} P -20.906, -20.730, -20.563 
{ c4  ADP-active 2} -21.961, -21.572, -20.868 
{ pa  ADP-active 2} P -17.492, -16.818, -20.503 
{ o3a ADP-active 2} -17.237, -15.958, -19.130 
{ c2* ADP-active 2} P -18.731, -21.770, -20.744 
{ c1* ADP-active 2} -19.824, -21.257, -19.736 
{ c5  ADP-active 2} P -22.766, -20.851, -21.708 
{ c4  ADP-active 2} -21.961, -21.572, -20.868 
{ n3  ADP-active 2} P -22.106, -22.823, -20.394 
{ c4  ADP-active 2} -21.961, -21.572, -20.868 
{ pb  ADP-active 1} P 17.207, 14.327, -19.200 
{ pb  ADP-active 1} 17.207, 14.327, -19.200 
{ o1b ADP-active 1} 18.467, 13.879, -19.870 
{ pb  ADP-active 1} P 17.207, 14.327, -19.200 
{ o2b ADP-active 1} 16.005, 14.060, -20.016 
{ pb  ADP-active 1} P 17.207, 14.327, -19.200 
{ o3b ADP-active 1} 17.257, 13.902, -17.775 
{ pa  ADP-active 1} P 17.492, 16.818, -20.503 
{ o1a ADP-active 1} 18.465, 16.093, -21.392 
{ pa  ADP-active 1} P 17.492, 16.818, -20.503 
{ o2a ADP-active 1} 16.225, 16.929, -21.270 
{ pb  ADP-active 1} P 17.207, 14.327, -19.200 
{ o3a ADP-active 1} 17.237, 15.958, -19.130 
{ pa  ADP-active 1} P 17.492, 16.818, -20.503 
{ o5* ADP-active 1} 18.125, 18.116, -19.886 
{ c5* ADP-active 1} 17.427, 18.813, -18.814 
{ c4* ADP-active 1} 17.740, 20.254, -19.260 
{ o4* ADP-active 1} 19.179, 20.339, -18.894 
{ c4* ADP-active 1} P 17.740, 20.254, -19.260 
{ c3* ADP-active 1} 17.857, 20.521, -20.760 
{ o3* ADP-active 1} 16.671, 20.680, -21.514 
{ c3* ADP-active 1} P 17.857, 20.521, -20.760 
{ c2* ADP-active 1} 18.731, 21.770, -20.744 
{ o2* ADP-active 1} 18.312, 23.057, -20.306 
{ o4* ADP-active 1} P 19.179, 20.339, -18.894 
{ c1* ADP-active 1} 19.824, 21.257, -19.736 
{ n9  ADP-active 1} 20.906, 20.730, -20.563 
{ c8  ADP-active 1} 21.078, 19.570, -21.251 
{ n7  ADP-active 1} 22.222, 19.602, -21.942 
{ c5  ADP-active 1} 22.766, 20.851, -21.708 
{ c6  ADP-active 1} 23.937, 21.502, -22.134 
{ n6  ADP-active 1} 24.828, 20.917, -22.933 
{ c6  ADP-active 1} P 23.937, 21.502, -22.134 
{ n1  ADP-active 1} 24.161, 22.776, -21.688 
{ c2  ADP-active 1} 23.220, 23.324, -20.855 
{ n3  ADP-active 1} 22.106, 22.823, -20.394 
{ n9  ADP-active 1} P 20.906, 20.730, -20.563 
{ c4  ADP-active 1} 21.961, 21.572, -20.868 
{ pa  ADP-active 1} P 17.492, 16.818, -20.503 
{ o3a ADP-active 1} 17.237, 15.958, -19.130 
{ c2* ADP-active 1} P 18.731, 21.770, -20.744 
{ c1* ADP-active 1} 19.824, 21.257, -19.736 
{ c5  ADP-active 1} P 22.766, 20.851, -21.708 
{ c4  ADP-active 1} 21.961, 21.572, -20.868 
{ n3  ADP-active 1} P 22.106, 22.823, -20.394 
{ c4  ADP-active 1} 21.961, 21.572, -20.868 
@balllist {Mg} color= green radius= 0.2
{Mg ADP-active 2} -17.052, -14.223, -22.318
{Mg ADP-active 1} 17.052, 14.223, -22.318
@subgroup {ADP-allo} dominant    
@vectorlist {ht} color= yellow     
{ pb  ADP-allo 1} P 8.236, -18.256, -8.053 
{ pb  ADP-allo 1} 8.236, -18.256, -8.053 
{ o1b ADP-allo 1} 7.491, -17.886, -6.833 
{ pb  ADP-allo 1} P 8.236, -18.256, -8.053 
{ o2b ADP-allo 1} 8.299, -17.230, -9.110 
{ pb  ADP-allo 1} P 8.236, -18.256, -8.053 
{ o3b ADP-allo 1} 9.553, -18.831, -7.688 
{ pa  ADP-allo 1} P 7.758, -20.451, -10.009 
{ o1a ADP-allo 1} 8.626, -21.615, -9.722 
{ pa  ADP-allo 1} P 7.758, -20.451, -10.009 
{ o2a ADP-allo 1} 8.437, -19.676, -11.093 
{ pb  ADP-allo 1} P 8.236, -18.256, -8.053 
{ o3a ADP-allo 1} 7.520, -19.557, -8.695 
{ pa  ADP-allo 1} P 7.758, -20.451, -10.009 
{ o5* ADP-allo 1} 6.284, -20.747, -10.477 
{ c5* ADP-allo 1} 5.266, -19.862, -11.015 
{ c4* ADP-allo 1} 3.943, -20.275, -10.394 
{ o4* ADP-allo 1} 3.781, -21.723, -10.572 
{ c4* ADP-allo 1} P 3.943, -20.275, -10.394 
{ c3* ADP-allo 1} 3.777, -20.025, -8.889 
{ o3* ADP-allo 1} 2.446, -19.902, -8.459 
{ c3* ADP-allo 1} P 3.777, -20.025, -8.889 
{ c2* ADP-allo 1} 4.317, -21.354, -8.315 
{ o2* ADP-allo 1} 3.992, -21.521, -6.952 
{ o4* ADP-allo 1} P 3.781, -21.723, -10.572 
{ c1* ADP-allo 1} 3.659, -22.346, -9.290 
{ n9  ADP-allo 1} 4.314, -23.659, -9.352 
{ c8  ADP-allo 1} 5.627, -23.876, -9.684 
{ n7  ADP-allo 1} 5.993, -25.142, -9.674 
{ c5  ADP-allo 1} P 4.845, -25.795, -9.301 
{ c6  ADP-allo 1} 4.614, -27.180, -9.109 
{ n6  ADP-allo 1} 5.563, -28.100, -9.278 
{ c6  ADP-allo 1} P 4.614, -27.180, -9.109 
{ n1  ADP-allo 1} 3.359, -27.517, -8.732 
{ c2  ADP-allo 1} 2.415, -26.525, -8.574 
{ n3  ADP-allo 1} 2.533, -25.237, -8.724 
{ n9  ADP-allo 1} P 4.314, -23.659, -9.352 
{ c4  ADP-allo 1} 3.800, -24.913, -9.096 
{ pa  ADP-allo 1} P 7.758, -20.451, -10.009 
{ o3a ADP-allo 1} 7.520, -19.557, -8.695 
{ c2* ADP-allo 1} P 4.317, -21.354, -8.315 
{ c1* ADP-allo 1} 3.659, -22.346, -9.290 
{ n7  ADP-allo 1} P 5.993, -25.142, -9.674 
{ c5  ADP-allo 1} 4.845, -25.795, -9.301 
{ c4  ADP-allo 1} 3.800, -24.913, -9.096 
{ n3  ADP-allo 1} P 2.533, -25.237, -8.724 
{ c4  ADP-allo 1} 3.800, -24.913, -9.096 
{ pb  ADP-allo 2} P -8.236, 18.256, -8.053 
{ pb  ADP-allo 2} -8.236, 18.256, -8.053 
{ o1b ADP-allo 2} -7.491, 17.886, -6.833 
{ pb  ADP-allo 2} P -8.236, 18.256, -8.053 
{ o2b ADP-allo 2} -8.299, 17.230, -9.110 
{ pb  ADP-allo 2} P -8.236, 18.256, -8.053 
{ o3b ADP-allo 2} -9.553, 18.831, -7.688 
{ pa  ADP-allo 2} P -7.758, 20.451, -10.009 
{ o1a ADP-allo 2} -8.626, 21.615, -9.722 
{ pa  ADP-allo 2} P -7.758, 20.451, -10.009 
{ o2a ADP-allo 2} -8.437, 19.676, -11.093 
{ pb  ADP-allo 2} P -8.236, 18.256, -8.053 
{ o3a ADP-allo 2} -7.520, 19.557, -8.695 
{ pa  ADP-allo 2} P -7.758, 20.451, -10.009 
{ o5* ADP-allo 2} -6.284, 20.747, -10.477 
{ c5* ADP-allo 2} -5.266, 19.862, -11.015 
{ c4* ADP-allo 2} -3.943, 20.275, -10.394 
{ o4* ADP-allo 2} -3.781, 21.723, -10.572 
{ c4* ADP-allo 2} P -3.943, 20.275, -10.394 
{ c3* ADP-allo 2} -3.777, 20.025, -8.889 
{ o3* ADP-allo 2} -2.446, 19.902, -8.459 
{ c3* ADP-allo 2} P -3.777, 20.025, -8.889 
{ c2* ADP-allo 2} -4.317, 21.354, -8.315 
{ o2* ADP-allo 2} -3.992, 21.521, -6.952 
{ o4* ADP-allo 2} P -3.781, 21.723, -10.572 
{ c1* ADP-allo 2} -3.659, 22.346, -9.290 
{ n9  ADP-allo 2} -4.314, 23.659, -9.352 
{ c8  ADP-allo 2} -5.627, 23.876, -9.684 
{ n7  ADP-allo 2} -5.993, 25.142, -9.674 
{ c5  ADP-allo 2} P -4.845, 25.795, -9.301 
{ c6  ADP-allo 2} -4.614, 27.180, -9.109 
{ n6  ADP-allo 2} -5.563, 28.100, -9.278 
{ c6  ADP-allo 2} P -4.614, 27.180, -9.109 
{ n1  ADP-allo 2} -3.359, 27.517, -8.732 
{ c2  ADP-allo 2} -2.415, 26.525, -8.574 
{ n3  ADP-allo 2} -2.533, 25.237, -8.724 
{ n9  ADP-allo 2} P -4.314, 23.659, -9.352 
{ c4  ADP-allo 2} -3.800, 24.913, -9.096 
{ pa  ADP-allo 2} P -7.758, 20.451, -10.009 
{ o3a ADP-allo 2} -7.520, 19.557, -8.695 
{ c2* ADP-allo 2} P -4.317, 21.354, -8.315 
{ c1* ADP-allo 2} -3.659, 22.346, -9.290 
{ n7  ADP-allo 2} P -5.993, 25.142, -9.674 
{ c5  ADP-allo 2} -4.845, 25.795, -9.301 
{ c4  ADP-allo 2} -3.800, 24.913, -9.096 
{ n3  ADP-allo 2} P -2.533, 25.237, -8.724 
{ c4  ADP-allo 2} -3.800, 24.913, -9.096 
@balllist color= yellow radius= 0.2
{Mg ADP-allo 1} 8.936, -17.383, -10.956
{Mg ADP-allo 2} -8.936, 17.383, -10.956
@group {T-State}  off  animate   
@subgroup {Dimer}    dominant
@vectorlist {Subunit 1} color= bluetint     
{ ca  met a 1 } P 27.325, 31.916, 2.606 
{ ca  met a 1 } 27.325, 31.916, 2.606 
{ ca  lys a 2 } 24.502, 34.147, 3.863 
{ ca  arg a 3 } 21.927, 32.848, 1.447 
{ ca  ile a 4 } 21.611, 30.043, -1.008 
{ ca  gly a 5 } 19.031, 28.744, -3.436 
{ ca  val a 6 } 17.624, 25.228, -4.202 
{ ca  leu a 7 } 15.987, 23.925, -7.352 
{ ca  thr a 8 } 14.511, 20.760, -8.936 
{ ca  ser a 9 } 14.988, 20.122, -12.660 
{ ca  gly a 10 } 14.666, 17.001, -14.790 
{ ca  gly a 11 } 11.520, 14.884, -14.472 
{ ca  asp a 12 } 10.545, 14.566, -10.806 
{ ca  ser a 13 } 11.125, 11.458, -8.701 
{ ca  pro a 14 } 9.850, 10.322, -5.281 
{ ca  gly a 15 } 12.216, 11.761, -2.639 
{ ca  met a 16 } 12.724, 15.173, -4.229 
{ ca  asn a 17 } 10.796, 16.828, -1.308 
{ ca  ala a 18 } 12.865, 14.922, 1.329 
{ ca  ala a 19 } 16.060, 16.534, -0.288 
{ ca  ile a 20 } 14.450, 19.999, -0.569 
{ ca  arg a 21 } 13.591, 19.545, 3.127 
{ ca  ser a 22 } 17.129, 18.438, 4.374 
{ ca  val a 23 } 18.589, 21.083, 1.964 
{ ca  val a 24 } 16.408, 23.834, 3.851 
{ ca  arg a 25 } 16.604, 22.626, 7.494 
{ ca  lys a 26 } 20.313, 22.127, 7.453 
{ ca  ala a 27 } 21.137, 25.476, 5.893 
{ ca  ile a 28 } 18.728, 27.058, 8.351 
{ ca  tyr a 29 } 20.372, 25.229, 11.245 
{ ca  his a 30 } 23.779, 26.815, 10.348 
{ ca  gly a 31 } 22.141, 30.193, 9.937 
{ ca  val a 32 } 21.936, 30.442, 6.060 
{ ca  glu a 33 } 18.901, 31.782, 4.200 
{ ca  val a 34 } 17.164, 29.547, 1.728 
{ ca  tyr a 35 } 15.324, 30.731, -1.313 
{ ca  gly a 36 } 13.444, 28.236, -3.588 
{ ca  val a 37 } 13.603, 28.621, -7.391 
{ ca  tyr a 38 } 10.330, 27.490, -8.845 
{ ca  his a 39 } 10.211, 25.693, -12.115 
{ ca  gly a 40 } 13.859, 24.515, -12.435 
{ ca  tyr a 41 } 16.107, 26.548, -14.742 
{ ca  ala a 42 } 13.209, 28.325, -16.424 
{ ca  gly a 43 } 12.067, 29.932, -13.151 
{ ca  leu a 44 } 15.687, 30.824, -12.363 
{ ca  ile a 45 } 15.938, 32.867, -15.531 
{ ca  ala a 46 } 12.526, 34.415, -14.902 
{ ca  gly a 47 } 13.338, 35.472, -11.304 
{ ca  asn a 48 } 10.787, 32.976, -9.854 
{ ca  ile a 49 } 12.604, 32.725, -6.596 
{ ca  lys a 50 } 11.118, 32.915, -3.103 
{ ca  lys a 51 } 12.300, 32.543, 0.441 
{ ca  leu a 52 } 11.510, 29.211, 2.168 
{ ca  glu a 53 } 11.243, 29.256, 6.013 
{ ca  val a 54 } 11.310, 26.262, 8.416 
{ ca  gly a 55 } 7.606, 25.535, 8.397 
{ ca  asp a 56 } 7.594, 25.713, 4.517 
{ ca  val a 57 } 9.343, 22.235, 4.303 
{ ca  gly a 58 } 6.946, 20.990, 7.013 
{ ca  asp a 59 } 4.632, 18.198, 5.757 
{ ca  ile a 60 } 6.404, 17.368, 2.525 
{ ca  ile a 61 } 8.847, 14.617, 3.625 
{ ca  his a 62 } 6.405, 11.855, 2.610 
{ ca  arg a 63 } 5.082, 13.597, -0.571 
{ ca  gly a 64 } 6.009, 12.430, -4.015 
{ ca  gly a 65 } 7.122, 14.885, -6.651 
{ ca  thr a 66 } 8.605, 18.337, -5.898 
{ ca  ile a 67 } 7.047, 21.460, -4.432 
{ ca  leu a 68 } 9.332, 23.778, -6.435 
{ ca  tyr a 69 } 8.244, 22.182, -9.711 
{ ca  thr a 70 } 10.494, 21.309, -12.617 
{ ca  ala a 71 } 10.393, 22.783, -16.101 
{ ca  arg a 72 } 12.216, 22.355, -19.379 
{ ca  cyh a 73 } 14.537, 25.202, -20.171 
{ ca  pro a 74 } 16.486, 25.168, -23.457 
{ ca  glu a 75 } 17.649, 28.809, -23.037 
{ ca  phe a 76 } 19.904, 27.721, -20.257 
{ ca  lys a 77 } 21.637, 25.879, -23.034 
{ ca  thr a 78 } 22.408, 29.414, -24.196 
{ ca  glu a 79 } 25.162, 31.529, -22.762 
{ ca  glu a 80 } 22.785, 34.437, -22.180 
{ ca  gly a 81 } 20.128, 32.188, -20.555 
{ ca  gln a 82 } 23.105, 31.191, -18.442 
{ ca  lys a 83 } 24.103, 34.818, -17.734 
{ ca  lys a 84 } 20.567, 35.837, -16.735 
{ ca  gly a 85 } 20.185, 32.955, -14.246 
{ ca  ile a 86 } 23.393, 34.497, -12.829 
{ ca  glu a 87 } 22.058, 38.033, -13.076 
{ ca  gln a 88 } 18.841, 36.870, -11.334 
{ ca  leu a 89 } 20.855, 35.142, -8.569 
{ ca  lys a 90 } 23.051, 38.186, -8.182 
{ ca  lys a 91 } 19.848, 40.266, -7.971 
{ ca  his a 92 } 18.300, 38.221, -5.106 
{ ca  gly a 93 } 21.555, 37.898, -3.129 
{ ca  ile a 94 } 21.870, 34.183, -3.554 
{ ca  gln a 95 } 25.463, 32.978, -3.082 
{ ca  gly a 96 } 25.300, 29.268, -3.959 
{ ca  leu a 97 } 22.921, 26.683, -5.292 
{ ca  val a 98 } 21.767, 23.142, -4.683 
{ ca  val a 99 } 20.485, 21.681, -8.002 
{ ca  ile a 100 } 18.462, 18.497, -7.379 
{ ca  gly a 101 } 17.695, 16.455, -10.599 
{ ca  gly a 102 } 19.289, 14.368, -13.336 
{ ca  asp a 103 } 22.006, 14.469, -16.046 
{ ca  gly a 104 } 20.635, 17.692, -17.572 
{ ca  ser a 105 } 20.491, 19.436, -14.184 
{ ca  tyr a 106 } 24.233, 18.658, -13.716 
{ ca  gln a 107 } 25.151, 20.111, -17.123 
{ ca  gly a 108 } 23.921, 23.540, -16.043 
{ ca  ala a 109 } 25.357, 23.262, -12.526 
{ ca  lys a 110 } 28.749, 22.767, -14.275 
{ ca  lys a 111 } 28.263, 25.725, -16.631 
{ ca  leu a 112 } 27.083, 27.891, -13.691 
{ ca  thr a 113 } 30.240, 27.060, -11.657 
{ ca  glu a 114 } 32.370, 27.604, -14.771 
{ ca  his a 115 } 30.775, 31.142, -14.488 
{ ca  gly a 116 } 32.106, 31.291, -10.900 
{ ca  phe a 117 } 28.745, 30.422, -9.432 
{ ca  pro a 118 } 29.004, 27.560, -6.916 
{ ca  cyh a 119 } 26.495, 24.786, -7.213 
{ ca  val a 120 } 26.329, 21.255, -5.882 
{ ca  gly a 121 } 24.267, 18.601, -7.792 
{ ca  val a 122 } 22.103, 15.954, -5.900 
{ ca  pro a 123 } 21.014, 12.872, -7.901 
{ ca  gly a 124 } 17.159, 13.088, -8.462 
{ ca  thr a 125 } 15.915, 10.443, -10.869 
{ ca  ile a 126 } 14.113, 6.982, -10.884 
{ ca  asp a 127 } 16.604, 5.716, -13.557 
{ ca  asn a 128 } 19.676, 5.132, -11.375 
{ ca  asp a 129 } 21.723, 6.521, -14.275 
{ ca  ile a 130 } 23.695, 9.392, -12.915 
{ ca  pro a 131 } 27.418, 8.669, -12.355 
{ ca  gly a 132 } 29.108, 9.113, -8.971 
{ ca  thr a 133 } 26.399, 7.398, -6.986 
{ ca  asp a 134 } 25.425, 3.767, -6.507 
{ ca  phe a 135 } 21.720, 4.945, -6.256 
{ ca  thr a 136 } 19.745, 8.135, -7.289 
{ ca  ile a 137 } 16.861, 9.372, -5.213 
{ ca  gly a 138 } 13.668, 7.766, -6.454 
{ ca  phe a 139 } 15.149, 4.520, -7.687 
{ ca  asp a 140 } 13.973, 2.554, -4.617 
{ ca  thr a 141 } 10.476, 3.922, -4.851 
{ ca  ala a 142 } 9.926, 3.200, -8.666 
{ ca  leu a 143 } 11.355, -0.366, -8.003 
{ ca  asn a 144 } 8.638, -0.904, -5.409 
{ ca  thr a 145 } 5.884, 0.543, -7.782 
{ ca  val a 146 } 6.903, -2.005, -10.278 
{ ca  ile a 147 } 6.973, -5.050, -7.963 
{ ca  asp a 148 } 3.395, -3.794, -6.783 
{ ca  ala a 149 } 2.061, -4.566, -10.335 
{ ca  ile a 150 } 4.141, -7.731, -10.621 
{ ca  asp a 151 } 2.657, -8.935, -7.330 
{ ca  lys a 152 } -0.927, -8.454, -8.054 
{ ca  ile a 153 } -0.453, -10.054, -11.500 
{ ca  arg a 154 } 0.976, -13.130, -9.666 
{ ca  asp a 155 } -2.531, -13.459, -8.133 
{ ca  thr a 156 } -4.442, -14.472, -11.346 
{ ca  ala a 157 } -5.408, -18.179, -11.002 
{ ca  thr a 158 } -6.445, -18.758, -14.641 
{ ca  ser a 159 } -5.038, -21.434, -16.925 
{ ca  his a 160 } -5.230, -19.729, -20.360 
{ ca  glu a 161 } -3.895, -16.284, -19.133 
{ ca  arg a 162 } -0.356, -17.309, -18.032 
{ ca  thr a 163 } 2.018, -14.938, -19.988 
{ ca  tyr a 164 } 2.575, -11.307, -18.971 
{ ca  val a 165 } 4.793, -8.541, -20.275 
{ ca  ile a 166 } 5.460, -5.548, -18.033 
{ ca  glu a 167 } 7.089, -2.549, -19.761 
{ ca  val a 168 } 9.174, -0.383, -17.355 
{ ca  met a 169 } 11.011, 2.955, -17.553 
{ ca  gly a 170 } 14.793, 2.999, -18.339 
{ ca  arg a 171 } 15.545, 4.466, -21.687 
{ ca  his a 172 } 19.256, 4.180, -21.334 
{ ca  ala a 173 } 19.765, 2.153, -18.260 
{ ca  gly a 174 } 18.392, -1.250, -17.256 
{ ca  asp a 175 } 18.400, -0.656, -13.455 
{ ca  ile a 176 } 14.560, -0.554, -12.967 
{ ca  ala a 177 } 14.170, -3.662, -15.279 
{ ca  leu a 178 } 17.076, -5.658, -13.629 
{ ca  trp a 179 } 16.048, -5.032, -9.951 
{ ca  ser a 180 } 12.119, -5.392, -10.482 
{ ca  gly a 181 } 12.848, -8.626, -12.584 
{ ca  leu a 182 } 14.991, -9.965, -9.789 
{ ca  ala a 183 } 12.630, -8.595, -7.043 
{ ca  gly a 184 } 9.637, -9.921, -9.080 
{ ca  gly a 185 } 10.797, -13.383, -10.183 
{ ca  ala a 186 } 10.653, -12.246, -13.806 
{ ca  glu a 187 } 11.730, -15.144, -16.157 
{ ca  thr a 188 } 13.160, -12.834, -18.877 
{ ca  ile a 189 } 14.478, -9.273, -18.483 
{ ca  leu a 190 } 15.413, -7.252, -21.605 
{ ca  ile a 191 } 17.778, -4.415, -20.756 
{ ca  pro a 192 } 19.908, -2.368, -23.157 
{ ca  glu a 193 } 23.170, -3.616, -21.593 
{ ca  ala a 194 } 22.572, -7.222, -22.492 
{ ca  asp a 195 } 21.981, -9.224, -25.628 
{ ca  tyr a 196 } 19.033, -11.522, -25.739 
{ ca  asp a 197 } 17.779, -14.170, -28.036 
{ ca  met a 198 } 14.088, -14.087, -28.902 
{ ca  asn a 199 } 14.384, -17.775, -29.783 
{ ca  asp a 200 } 15.973, -18.231, -26.356 
{ ca  val a 201 } 13.365, -16.054, -24.735 
{ ca  ile a 202 } 10.723, -18.306, -26.405 
{ ca  ala a 203 } 12.561, -21.586, -25.694 
{ ca  arg a 204 } 12.829, -21.013, -22.053 
{ ca  leu a 205 } 9.300, -19.728, -22.067 
{ ca  lys a 206 } 7.982, -23.141, -23.318 
{ ca  arg a 207 } 10.173, -25.093, -20.928 
{ ca  gly a 208 } 8.813, -23.204, -17.823 
{ ca  his a 209 } 5.348, -23.921, -19.202 
{ ca  glu a 210 } 6.202, -27.635, -19.734 
{ ca  arg a 211 } 7.434, -27.785, -16.140 
{ ca  gly a 212 } 4.154, -26.191, -15.029 
{ ca  lys a 213 } 5.349, -22.866, -13.719 
{ ca  lys a 214 } 2.380, -20.842, -12.559 
{ ca  his a 215 } 3.319, -17.840, -14.716 
{ ca  ser a 216 } 5.915, -16.527, -17.117 
{ ca  ile a 217 } 6.695, -12.879, -16.414 
{ ca  ile a 218 } 8.759, -10.791, -18.883 
{ ca  ile a 219 } 10.138, -7.290, -18.147 
{ ca  val a 220 } 11.122, -4.950, -20.942 
{ ca  ala a 221 } 13.022, -1.715, -20.152 
{ ca  glu a 222 } 11.473, 0.849, -22.573 
{ ca  gly a 223 } 14.885, 1.630, -24.239 
{ ca  val a 224 } 14.948, -1.946, -25.637 
{ ca  gly a 225 } 11.422, -2.023, -27.219 
{ ca  ser a 226 } 7.655, -1.873, -26.573 
{ ca  gly a 227 } 5.813, -4.108, -24.193 
{ ca  val a 228 } 3.002, -4.315, -26.800 
{ ca  asp a 229 } 5.078, -5.277, -29.809 
{ ca  phe a 230 } 6.773, -7.802, -27.560 
{ ca  gly a 231 } 3.456, -9.380, -26.577 
{ ca  arg a 232 } 2.375, -9.633, -30.230 
{ ca  gln a 233 } 5.453, -11.645, -31.098 
{ ca  ile a 234 } 5.552, -13.963, -28.057 
{ ca  gln a 235 } 1.928, -14.812, -28.657 
{ ca  glu a 236 } 2.375, -15.007, -32.442 
{ ca  ala a 237 } 5.459, -17.224, -31.794 
{ ca  thr a 238 } 4.192, -19.514, -29.036 
{ ca  gly a 239 } 0.441, -19.012, -29.612 
{ ca  phe a 240 } 0.360, -18.497, -25.814 
{ ca  glu a 241 } -2.015, -15.860, -24.477 
{ ca  thr a 242 } 0.015, -12.727, -23.725 
{ ca  arg a 243 } -1.117, -9.889, -21.455 
{ ca  val a 244 } 0.592, -6.523, -21.429 
{ ca  thr a 245 } 0.868, -3.746, -18.850 
{ ca  val a 246 } 2.623, -0.352, -19.405 
{ ca  leu a 247 } 3.134, 1.508, -16.007 
{ ca  gly a 248 } 4.168, 4.812, -17.681 
{ ca  his a 249 } 4.479, 7.743, -15.209 
{ ca  val a 250 } 3.037, 6.031, -12.148 
{ ca  gln a 251 } 6.718, 4.879, -11.888 
{ ca  arg a 252 } 7.793, 8.485, -11.246 
{ ca  gly a 253 } 5.208, 9.221, -8.677 
{ ca  gly a 254 } 4.132, 8.300, -5.223 
{ ca  ser a 255 } 5.384, 8.754, -1.605 
{ ca  pro a 256 } 9.139, 8.043, -1.297 
{ ca  thr a 257 } 10.155, 4.673, 0.374 
{ ca  ala a 258 } 12.142, 4.731, 3.722 
{ ca  phe a 259 } 15.471, 4.329, 1.727 
{ ca  asp a 260 } 14.790, 7.361, -0.700 
{ ca  arg a 261 } 13.913, 9.430, 2.342 
{ ca  val a 262 } 17.098, 8.483, 4.360 
{ ca  leu a 263 } 19.061, 8.844, 1.123 
{ ca  ala a 264 } 17.619, 12.413, 0.206 
{ ca  ser a 265 } 18.145, 13.706, 3.752 
{ ca  arg a 266 } 21.779, 12.694, 3.864 
{ ca  leu a 267 } 22.681, 13.932, 0.354 
{ ca  gly a 268 } 20.682, 17.156, 0.598 
{ ca  ala a 269 } 22.481, 18.116, 3.903 
{ ca  arg a 270 } 25.864, 17.073, 2.518 
{ ca  ala a 271 } 25.327, 19.401, -0.466 
{ ca  val a 272 } 24.785, 22.340, 1.949 
{ ca  glu a 273 } 27.972, 21.488, 3.907 
{ ca  leu a 274 } 29.870, 21.339, 0.626 
{ ca  leu a 275 } 28.736, 24.958, -0.225 
{ ca  leu a 276 } 29.623, 26.152, 3.307 
{ ca  glu a 277 } 33.176, 24.600, 3.251 
{ ca  gly a 278 } 33.741, 26.431, 0.007 
{ ca  lys a 279 } 33.689, 23.227, -2.282 
{ ca  gly a 280 } 31.162, 24.160, -5.056 
{ ca  gly a 281 } 31.109, 22.591, -8.614 
{ ca  arg a 282 } 30.672, 19.073, -7.060 
{ ca  cyh a 283 } 27.962, 16.375, -7.172 
{ ca  val a 284 } 26.976, 14.304, -4.125 
{ ca  gly a 285 } 26.293, 10.595, -4.164 
{ ca  ile a 286 } 26.495, 7.246, -2.242 
{ ca  gln a 287 } 29.260, 4.722, -3.181 
{ ca  asn a 288 } 30.330, 1.630, -1.433 
{ ca  asn a 289 } 27.872, 2.563, 1.209 
{ ca  gln a 290 } 29.542, 5.854, 1.918 
{ ca  leu a 291 } 28.664, 9.533, 1.206 
{ ca  val a 292 } 31.011, 10.751, -1.608 
{ ca  asp a 293 } 31.208, 14.000, -3.607 
{ ca  his a 294 } 32.964, 14.271, -6.985 
{ ca  asp a 295 } 33.780, 17.010, -9.390 
{ ca  ile a 296 } 30.793, 17.477, -11.786 
{ ca  ala a 297 } 32.813, 17.172, -15.020 
{ ca  glu a 298 } 34.756, 14.274, -13.713 
{ ca  ala a 299 } 31.609, 12.473, -12.682 
{ ca  leu a 300 } 29.906, 13.325, -15.957 
{ ca  ala a 302 } 32.738, 11.658, -17.966 
{ ca  asn a 303 } 31.992, 8.293, -16.320 
{ ca  lys a 304 } 29.887, 5.550, -17.745 
{ ca  his a 305 } 26.836, 3.938, -16.252 
{ ca  thr a 306 } 26.940, 0.196, -15.589 
{ ca  ile a 307 } 24.839, -2.771, -14.437 
{ ca  asp a 308 } 26.090, -5.715, -12.339 
{ ca  gln a 309 } 26.250, -8.693, -14.722 
{ ca  arg a 310 } 26.395, -11.159, -11.816 
{ ca  met a 311 } 22.856, -9.917, -10.959 
{ ca  tyr a 312 } 21.749, -10.262, -14.548 
{ ca  ala a 313 } 23.183, -13.815, -14.759 
{ ca  leu a 314 } 21.734, -14.660, -11.336 
{ ca  ser a 315 } 18.195, -13.718, -12.594 
{ ca  lys a 316 } 18.497, -16.555, -15.129 
{ ca  glu a 317 } 19.740, -19.054, -12.557 
{ ca  leu a 318 } 16.771, -18.385, -10.320 
{ ca  ser a 319 } 13.973, -18.449, -12.986 
{ ca  ile a 320 } 14.211, -22.205, -13.782 
@vectorlist {Subunit 2} color= sky     
{ ca  met b 1 } P -28.183, -31.494, 3.073 
{ ca  met b 1 } -28.183, -31.494, 3.073 
{ ca  lys b 2 } -25.249, -33.672, 3.847 
{ ca  arg b 3 } -22.599, -32.643, 1.348 
{ ca  ile b 4 } -22.247, -29.666, -1.033 
{ ca  gly b 5 } -19.578, -28.453, -3.624 
{ ca  val b 6 } -18.014, -25.031, -4.260 
{ ca  leu b 7 } -16.427, -23.964, -7.560 
{ ca  thr b 8 } -14.973, -20.698, -8.906 
{ ca  ser b 9 } -15.458, -19.915, -12.611 
{ ca  gly b 10 } -14.830, -17.010, -14.999 
{ ca  gly b 11 } -11.769, -14.764, -14.614 
{ ca  asp b 12 } -10.696, -14.572, -10.976 
{ ca  ser b 13 } -11.297, -11.655, -8.718 
{ ca  pro b 14 } -9.842, -10.655, -5.393 
{ ca  gly b 15 } -12.235, -11.824, -2.691 
{ ca  met b 16 } -12.856, -15.192, -4.316 
{ ca  asn b 17 } -10.895, -16.810, -1.459 
{ ca  ala b 18 } -12.966, -14.998, 1.199 
{ ca  ala b 19 } -16.176, -16.408, -0.371 
{ ca  ile b 20 } -14.898, -20.025, -0.511 
{ ca  arg b 21 } -13.831, -19.675, 3.163 
{ ca  ser b 22 } -17.293, -18.484, 4.213 
{ ca  val b 23 } -18.914, -21.217, 2.051 
{ ca  val b 24 } -16.854, -24.033, 3.834 
{ ca  arg b 25 } -17.149, -22.542, 7.416 
{ ca  lys b 26 } -20.952, -21.872, 7.293 
{ ca  ala b 27 } -21.678, -25.379, 5.890 
{ ca  ile b 28 } -19.443, -27.086, 8.504 
{ ca  tyr b 29 } -21.017, -24.900, 11.241 
{ ca  his b 30 } -24.319, -26.485, 10.256 
{ ca  gly b 31 } -22.802, -30.003, 9.867 
{ ca  val b 32 } -22.545, -30.139, 6.011 
{ ca  glu b 33 } -19.364, -31.438, 4.216 
{ ca  val b 34 } -17.886, -29.288, 1.471 
{ ca  tyr b 35 } -15.970, -30.622, -1.440 
{ ca  gly b 36 } -13.966, -28.223, -3.668 
{ ca  val b 37 } -14.117, -28.579, -7.500 
{ ca  tyr b 38 } -10.909, -27.544, -9.112 
{ ca  his b 39 } -10.912, -25.641, -12.345 
{ ca  gly b 40 } -14.497, -24.594, -12.481 
{ ca  tyr b 41 } -16.637, -26.637, -14.831 
{ ca  ala b 42 } -13.765, -28.322, -16.629 
{ ca  gly b 43 } -12.615, -29.816, -13.377 
{ ca  leu b 44 } -16.217, -30.670, -12.461 
{ ca  ile b 45 } -16.733, -32.538, -15.751 
{ ca  ala b 46 } -13.292, -34.020, -15.092 
{ ca  gly b 47 } -13.968, -35.341, -11.524 
{ ca  asn b 48 } -11.345, -33.223, -9.760 
{ ca  ile b 49 } -13.431, -32.685, -6.695 
{ ca  lys b 50 } -11.653, -32.935, -3.304 
{ ca  lys b 51 } -13.003, -32.663, 0.239 
{ ca  leu b 52 } -12.420, -29.347, 2.105 
{ ca  glu b 53 } -11.807, -29.273, 5.884 
{ ca  val b 54 } -11.962, -26.334, 8.342 
{ ca  gly b 55 } -8.196, -25.787, 8.262 
{ ca  asp b 56 } -8.230, -26.036, 4.406 
{ ca  val b 57 } -9.579, -22.503, 4.012 
{ ca  gly b 58 } -7.360, -21.219, 6.817 
{ ca  asp b 59 } -5.121, -18.289, 5.792 
{ ca  ile b 60 } -6.845, -17.512, 2.441 
{ ca  ile b 61 } -9.042, -14.672, 3.617 
{ ca  his b 62 } -6.457, -11.990, 2.542 
{ ca  arg b 63 } -5.148, -13.647, -0.716 
{ ca  gly b 64 } -6.267, -12.461, -4.156 
{ ca  gly b 65 } -7.070, -15.134, -6.817 
{ ca  thr b 66 } -8.713, -18.425, -6.099 
{ ca  ile b 67 } -7.387, -21.708, -4.675 
{ ca  leu b 68 } -9.833, -23.934, -6.587 
{ ca  tyr b 69 } -8.605, -22.292, -9.767 
{ ca  thr b 70 } -10.901, -21.324, -12.601 
{ ca  ala b 71 } -10.800, -22.683, -16.107 
{ ca  arg b 72 } -12.455, -22.458, -19.519 
{ ca  cyh b 73 } -15.042, -25.252, -20.323 
{ ca  pro b 74 } -17.090, -24.752, -23.522 
{ ca  glu b 75 } -18.695, -28.164, -23.373 
{ ca  phe b 76 } -20.466, -27.422, -20.193 
{ ca  lys b 77 } -22.288, -25.494, -22.838 
{ ca  thr b 78 } -22.957, -29.005, -24.320 
{ ca  glu b 79 } -25.623, -31.314, -22.925 
{ ca  glu b 80 } -23.202, -34.208, -22.662 
{ ca  gly b 81 } -20.720, -31.821, -20.957 
{ ca  gln b 82 } -23.541, -31.037, -18.504 
{ ca  lys b 83 } -24.373, -34.749, -18.206 
{ ca  lys b 84 } -20.800, -35.749, -17.238 
{ ca  gly b 85 } -20.859, -32.823, -14.777 
{ ca  ile b 86 } -23.899, -34.390, -13.006 
{ ca  glu b 87 } -22.445, -37.948, -13.249 
{ ca  gln b 88 } -19.426, -36.771, -11.144 
{ ca  leu b 89 } -21.361, -34.905, -8.404 
{ ca  lys b 90 } -23.478, -37.939, -7.680 
{ ca  lys b 91 } -20.502, -40.268, -7.937 
{ ca  his b 92 } -19.065, -38.031, -5.231 
{ ca  gly b 93 } -22.348, -37.835, -3.257 
{ ca  ile b 94 } -22.604, -34.053, -3.592 
{ ca  gln b 95 } -26.212, -32.881, -3.417 
{ ca  gly b 96 } -25.999, -29.063, -4.249 
{ ca  leu b 97 } -23.389, -26.607, -5.447 
{ ca  val b 98 } -22.206, -23.020, -4.959 
{ ca  val b 99 } -20.796, -21.170, -8.007 
{ ca  ile b 100 } -18.580, -18.047, -7.534 
{ ca  gly b 101 } -17.947, -16.160, -10.788 
{ ca  gly b 102 } -19.477, -14.247, -13.698 
{ ca  asp b 103 } -22.738, -14.465, -15.550 
{ ca  gly b 104 } -20.856, -17.088, -17.608 
{ ca  ser b 105 } -20.754, -19.382, -14.515 
{ ca  tyr b 106 } -24.476, -18.458, -13.897 
{ ca  gln b 107 } -25.550, -20.016, -17.179 
{ ca  gly b 108 } -24.334, -23.389, -15.943 
{ ca  ala b 109 } -25.953, -22.737, -12.536 
{ ca  lys b 110 } -29.271, -22.399, -14.429 
{ ca  lys b 111 } -28.730, -25.545, -16.577 
{ ca  leu b 112 } -27.623, -27.606, -13.631 
{ ca  thr b 113 } -30.742, -26.453, -11.817 
{ ca  glu b 114 } -32.885, -27.127, -14.878 
{ ca  his b 115 } -31.214, -30.642, -14.959 
{ ca  gly b 116 } -32.512, -30.930, -11.467 
{ ca  phe b 117 } -29.332, -29.955, -9.567 
{ ca  pro b 118 } -29.501, -27.175, -6.943 
{ ca  cyh b 119 } -26.960, -24.484, -7.513 
{ ca  val b 120 } -26.647, -20.952, -6.096 
{ ca  gly b 121 } -24.393, -18.492, -8.039 
{ ca  val b 122 } -22.167, -15.895, -6.131 
{ ca  pro b 123 } -21.203, -12.636, -7.837 
{ ca  gly b 124 } -17.407, -12.813, -8.388 
{ ca  thr b 125 } -16.034, -10.403, -11.001 
{ ca  ile b 126 } -14.075, -7.059, -11.018 
{ ca  asp b 127 } -16.434, -5.526, -13.673 
{ ca  asn b 128 } -19.483, -5.195, -11.462 
{ ca  asp b 129 } -21.400, -6.410, -14.576 
{ ca  ile b 130 } -23.603, -9.193, -12.982 
{ ca  pro b 131 } -27.316, -8.438, -12.319 
{ ca  gly b 132 } -29.230, -8.354, -9.140 
{ ca  thr b 133 } -26.370, -7.103, -7.048 
{ ca  asp b 134 } -25.271, -3.466, -6.678 
{ ca  phe b 135 } -21.704, -4.732, -6.318 
{ ca  thr b 136 } -19.814, -7.698, -7.380 
{ ca  ile b 137 } -16.918, -9.311, -5.488 
{ ca  gly b 138 } -13.842, -7.825, -6.679 
{ ca  phe b 139 } -15.109, -4.462, -7.701 
{ ca  asp b 140 } -13.796, -2.466, -4.708 
{ ca  thr b 141 } -10.284, -4.096, -4.956 
{ ca  ala b 142 } -10.159, -3.318, -8.516 
{ ca  leu b 143 } -11.179, 0.472, -8.024 
{ ca  asn b 144 } -8.449, 0.877, -5.373 
{ ca  thr b 145 } -5.804, -0.660, -7.868 
{ ca  val b 146 } -6.735, 2.017, -10.370 
{ ca  ile b 147 } -6.816, 5.058, -7.993 
{ ca  asp b 148 } -3.496, 3.946, -6.580 
{ ca  ala b 149 } -2.119, 4.413, -9.985 
{ ca  ile b 150 } -3.939, 7.744, -10.542 
{ ca  asp b 151 } -2.617, 9.105, -7.296 
{ ca  lys b 152 } 0.927, 8.103, -8.414 
{ ca  ile b 153 } 0.472, 10.096, -11.737 
{ ca  arg b 154 } -0.985, 13.004, -9.687 
{ ca  asp b 155 } 2.550, 13.614, -8.171 
{ ca  thr b 156 } 4.297, 14.117, -11.618 
{ ca  ala b 157 } 4.719, 17.983, -11.251 
{ ca  thr b 158 } 6.055, 18.926, -14.759 
{ ca  ser b 159 } 3.896, 21.395, -16.805 
{ ca  his b 160 } 5.121, 19.885, -20.140 
{ ca  glu b 161 } 3.606, 16.591, -19.196 
{ ca  arg b 162 } 0.126, 17.255, -17.838 
{ ca  thr b 163 } -2.037, 14.927, -20.010 
{ ca  tyr b 164 } -2.471, 11.394, -18.837 
{ ca  val b 165 } -4.705, 8.778, -20.295 
{ ca  ile b 166 } -5.336, 5.704, -18.212 
{ ca  glu b 167 } -6.839, 2.692, -19.791 
{ ca  val b 168 } -8.870, 0.645, -17.257 
{ ca  met b 169 } -10.665, -2.717, -17.632 
{ ca  gly b 170 } -14.416, -3.157, -18.378 
{ ca  arg b 171 } -15.409, -4.515, -21.799 
{ ca  his b 172 } -19.121, -3.955, -21.362 
{ ca  ala b 173 } -19.622, -2.087, -18.111 
{ ca  gly b 174 } -18.159, 1.358, -17.324 
{ ca  asp b 175 } -18.418, 0.917, -13.447 
{ ca  ile b 176 } -14.645, 0.968, -12.968 
{ ca  ala b 177 } -13.803, 3.940, -15.261 
{ ca  leu b 178 } -16.665, 5.912, -13.486 
{ ca  trp b 179 } -16.053, 5.269, -9.803 
{ ca  ser b 180 } -12.319, 5.437, -10.375 
{ ca  gly b 181 } -12.593, 8.628, -12.423 
{ ca  leu b 182 } -14.804, 10.263, -9.811 
{ ca  ala b 183 } -12.633, 9.135, -6.922 
{ ca  gly b 184 } -9.441, 10.030, -8.842 
{ ca  gly b 185 } -10.560, 13.474, -10.134 
{ ca  ala b 186 } -10.603, 12.423, -13.802 
{ ca  glu b 187 } -11.596, 15.254, -16.095 
{ ca  thr b 188 } -12.847, 12.914, -18.801 
{ ca  ile b 189 } -14.398, 9.463, -18.311 
{ ca  leu b 190 } -15.103, 7.408, -21.540 
{ ca  ile b 191 } -17.578, 4.682, -20.967 
{ ca  pro b 192 } -19.336, 2.203, -23.231 
{ ca  glu b 193 } -22.746, 3.474, -22.045 
{ ca  ala b 194 } -22.266, 7.208, -22.727 
{ ca  asp b 195 } -21.695, 9.019, -25.928 
{ ca  tyr b 196 } -18.970, 11.548, -25.893 
{ ca  asp b 197 } -17.646, 14.133, -28.257 
{ ca  met b 198 } -13.928, 14.087, -28.974 
{ ca  asn b 199 } -14.205, 17.852, -29.589 
{ ca  asp b 200 } -15.922, 18.233, -26.253 
{ ca  val b 201 } -13.003, 16.223, -24.701 
{ ca  ile b 202 } -10.490, 18.485, -26.493 
{ ca  ala b 203 } -12.522, 21.542, -25.505 
{ ca  arg b 204 } -12.360, 20.720, -21.753 
{ ca  leu b 205 } -8.615, 19.998, -21.842 
{ ca  lys b 206 } -8.013, 23.404, -23.417 
{ ca  arg b 207 } -10.246, 25.057, -20.791 
{ ca  gly b 208 } -8.614, 23.239, -17.811 
{ ca  his b 209 } -5.231, 24.199, -19.222 
{ ca  glu b 210 } -6.298, 27.878, -19.483 
{ ca  arg b 211 } -7.146, 28.085, -15.821 
{ ca  gly b 212 } -3.880, 26.423, -14.976 
{ ca  lys b 213 } -5.175, 23.055, -13.768 
{ ca  lys b 214 } -2.187, 20.927, -12.709 
{ ca  his b 215 } -3.288, 17.788, -14.548 
{ ca  ser b 216 } -5.749, 16.682, -17.176 
{ ca  ile b 217 } -6.328, 12.954, -16.437 
{ ca  ile b 218 } -8.558, 10.974, -18.788 
{ ca  ile b 219 } -9.860, 7.485, -17.971 
{ ca  val b 220 } -10.841, 5.162, -20.880 
{ ca  ala b 221 } -12.827, 1.926, -20.448 
{ ca  ala b 221 } -12.827, 1.926, -20.448 
{ ca  glu b 222 } -11.245, -0.776, -22.585 
{ ca  gly b 223 } -14.718, -1.344, -24.139 
{ ca  val b 224 } -14.462, 2.239, -25.691 
{ ca  gly b 225 } -10.993, 1.911, -27.307 
{ ca  ser b 226 } -7.219, 1.862, -26.829 
{ ca  gly b 227 } -5.763, 4.341, -24.314 
{ ca  val b 228 } -2.862, 4.340, -26.806 
{ ca  asp b 229 } -4.943, 5.541, -29.788 
{ ca  phe b 230 } -6.758, 8.008, -27.589 
{ ca  gly b 231 } -3.429, 9.336, -26.409 
{ ca  arg b 232 } -1.941, 9.508, -29.933 
{ ca  gln b 233 } -5.043, 11.572, -30.994 
{ ca  ile b 234 } -5.214, 13.992, -28.053 
{ ca  gln b 235 } -1.557, 14.654, -28.823 
{ ca  glu b 236 } -2.092, 15.150, -32.587 
{ ca  ala b 237 } -5.183, 17.270, -32.192 
{ ca  thr b 238 } -3.897, 19.514, -29.374 
{ ca  gly b 239 } -0.121, 19.081, -29.537 
{ ca  phe b 240 } -0.044, 18.480, -25.755 
{ ca  glu b 241 } 2.440, 15.983, -24.543 
{ ca  thr b 242 } 0.281, 13.024, -23.832 
{ ca  arg b 243 } 1.141, 10.105, -21.568 
{ ca  val b 244 } -0.505, 6.673, -21.252 
{ ca  thr b 245 } -0.739, 3.919, -18.640 
{ ca  val b 246 } -2.483, 0.629, -19.445 
{ ca  leu b 247 } -2.856, -1.401, -16.181 
{ ca  gly b 248 } -4.049, -4.662, -17.831 
{ ca  his b 249 } -4.280, -7.685, -15.500 
{ ca  val b 250 } -2.928, -5.808, -12.448 
{ ca  gln b 251 } -6.644, -4.975, -12.043 
{ ca  arg b 252 } -7.432, -8.675, -11.395 
{ ca  gly b 253 } -5.058, -9.455, -8.504 
{ ca  gly b 254 } -3.921, -8.128, -5.258 
{ ca  ser b 255 } -5.339, -8.751, -1.829 
{ ca  pro b 256 } -9.066, -7.949, -1.504 
{ ca  thr b 257 } -10.103, -4.855, 0.317 
{ ca  ala b 258 } -12.155, -4.744, 3.545 
{ ca  phe b 259 } -15.449, -4.270, 1.629 
{ ca  asp b 260 } -14.646, -7.040, -0.744 
{ ca  arg b 261 } -13.893, -9.293, 2.291 
{ ca  val b 262 } -17.261, -8.377, 3.934 
{ ca  leu b 263 } -19.287, -8.835, 0.714 
{ ca  ala b 264 } -17.457, -12.180, -0.130 
{ ca  ser b 265 } -17.931, -13.421, 3.534 
{ ca  arg b 266 } -21.703, -12.321, 3.678 
{ ca  leu b 267 } -22.858, -13.676, 0.076 
{ ca  gly b 268 } -20.968, -17.026, 0.527 
{ ca  ala b 269 } -22.844, -17.559, 3.760 
{ ca  arg b 270 } -26.318, -16.507, 2.281 
{ ca  ala b 271 } -25.631, -19.038, -0.557 
{ ca  val b 272 } -25.107, -22.021, 1.982 
{ ca  glu b 273 } -28.402, -20.885, 3.634 
{ ca  leu b 274 } -30.503, -20.917, 0.379 
{ ca  leu b 275 } -29.135, -24.465, -0.467 
{ ca  leu b 276 } -29.873, -25.582, 3.109 
{ ca  glu b 277 } -33.431, -24.238, 2.961 
{ ca  gly b 278 } -34.253, -26.071, -0.303 
{ ca  lys b 279 } -33.973, -23.006, -2.630 
{ ca  gly b 280 } -31.734, -23.795, -5.593 
{ ca  gly b 281 } -31.511, -21.870, -8.919 
{ ca  arg b 282 } -30.981, -18.593, -7.132 
{ ca  cyh b 283 } -28.266, -16.001, -7.181 
{ ca  val b 284 } -27.345, -13.818, -4.184 
{ ca  gly b 285 } -26.315, -10.063, -4.306 
{ ca  ile b 286 } -26.543, -6.860, -2.347 
{ ca  gln b 287 } -29.183, -4.322, -3.319 
{ ca  asn b 288 } -30.117, -1.124, -1.625 
{ ca  asn b 289 } -27.654, -2.296, 0.959 
{ ca  gln b 290 } -29.410, -5.598, 1.579 
{ ca  leu b 291 } -28.796, -9.262, 0.913 
{ ca  val b 292 } -31.149, -10.478, -1.839 
{ ca  asp b 293 } -31.366, -13.665, -3.945 
{ ca  his b 294 } -33.188, -13.850, -7.284 
{ ca  asp b 295 } -33.976, -16.624, -9.719 
{ ca  ile b 296 } -30.892, -17.079, -11.905 
{ ca  ala b 297 } -32.934, -17.021, -15.053 
{ ca  glu b 298 } -34.689, -13.662, -14.488 
{ ca  ala b 299 } -31.748, -12.108, -12.673 
{ ca  leu b 300 } -30.039, -12.929, -16.027 
{ ca  ala b 302 } -32.998, -11.294, -17.886 
{ ca  asn b 303 } -31.939, -7.882, -16.647 
{ ca  lys b 304 } -29.734, -5.222, -17.989 
{ ca  his b 305 } -26.830, -3.667, -16.234 
{ ca  thr b 306 } -27.002, 0.139, -16.096 
{ ca  ile b 307 } -24.782, 2.913, -14.634 
{ ca  asp b 308 } -25.658, 5.867, -12.501 
{ ca  gln b 309 } -26.169, 8.727, -14.925 
{ ca  arg b 310 } -26.120, 11.140, -12.037 
{ ca  met b 311 } -22.629, 10.081, -10.879 
{ ca  tyr b 312 } -21.461, 10.382, -14.491 
{ ca  ala b 313 } -22.940, 13.953, -14.574 
{ ca  leu b 314 } -21.343, 14.836, -11.309 
{ ca  ser b 315 } -17.893, 13.681, -12.476 
{ ca  lys b 316 } -18.155, 16.421, -15.091 
{ ca  glu b 317 } -19.320, 19.145, -12.626 
{ ca  leu b 318 } -16.485, 18.572, -10.089 
{ ca  ser b 319 } -13.728, 18.562, -12.764 
{ ca  ile b 320 } -14.107, 22.330, -13.430 
@subgroup {Glu 161} dominant    
@vectorlist {sc} color= red     
{ cd  glu a 161 } P -6.782, -14.277, -19.204 
{ oe2 glu a 161 } -6.145, -14.256, -20.287 
{ cd  glu a 161 } P -6.782, -14.277, -19.204 
{ oe1 glu a 161 } -7.911, -13.811, -19.118 
{ cg  glu a 161 } P -6.004, -14.918, -18.087 
{ cd  glu a 161 } -6.782, -14.277, -19.204 
{ cb  glu a 161 } P -4.537, -15.106, -18.434 
{ cg  glu a 161 } -6.004, -14.918, -18.087 
{ ca  glu a 161 } P -3.895, -16.284, -19.133 
{ cb  glu a 161 } -4.537, -15.106, -18.434 
{ cd  glu b 161 } P 6.898, 14.637, -19.351 
{ oe2 glu b 161 } 7.084, 13.586, -19.983 
{ cd  glu b 161 } P 6.898, 14.637, -19.351 
{ oe1 glu b 161 } 7.568, 15.034, -18.408 
{ cg  glu b 161 } P 5.712, 15.372, -19.886 
{ cd  glu b 161 } 6.898, 14.637, -19.351 
{ cb  glu b 161 } P 4.562, 15.461, -18.858 
{ cg  glu b 161 } 5.712, 15.372, -19.886 
{ ca  glu b 161 } P 3.606, 16.591, -19.196 
{ cb  glu b 161 } 4.562, 15.461, -18.858 
@subgroup {Arg 162} dominant    
@vectorlist {sc} color= cyan     
{ ca  arg a 162 } P -0.356, -17.309, -18.032 
{ cb  arg a 162 } -0.092, -18.797, -17.780 
{ cz  arg b 162 } P -1.865, 22.643, -17.432 
{ nh2 arg b 162 } -3.160, 22.968, -17.566 
{ cz  arg b 162 } P -1.865, 22.643, -17.432 
{ nh1 arg b 162 } -0.976, 23.575, -17.082 
{ ne  arg b 162 } P -1.634, 21.356, -17.790 
{ cz  arg b 162 } -1.865, 22.643, -17.432 
{ cd  arg b 162 } P -0.219, 20.977, -17.935 
{ ne  arg b 162 } -1.634, 21.356, -17.790 
{ cg  arg b 162 } P -0.111, 19.701, -18.746 
{ cd  arg b 162 } -0.219, 20.977, -17.935 
{ cb  arg b 162 } P -0.418, 18.658, -17.671 
{ cg  arg b 162 } -0.111, 19.701, -18.746 
{ ca  arg b 162 } P 0.126, 17.255, -17.838 
{ cb  arg b 162 } -0.418, 18.658, -17.671 
@subgroup {PGC} dominant    
@vectorlist {ht} color= gold     
{ p1  PGC 1} P 8.239, -18.215, -9.388 
{ p1  PGC 1} 8.239, -18.215, -9.388 
{ o1  PGC 1} 9.702, -18.494, -9.273 
{ p1  PGC 1} P 8.239, -18.215, -9.388 
{ o2  PGC 1} 8.116, -17.240, -10.518 
{ p1  PGC 1} P 8.239, -18.215, -9.388 
{ o3  PGC 1} 7.697, -17.698, -8.068 
{ p1  PGC 1} P 8.239, -18.215, -9.388 
{ o4  PGC 1} 7.238, -19.470, -9.545 
{ c1  PGC 1} 7.454, -20.438, -10.555 
{ c2  PGC 1} 6.546, -21.642, -10.232 
{ o5  PGC 1} 7.102, -22.847, -10.013 
{ c2  PGC 1} P 6.546, -21.642, -10.232 
{ o6  PGC 1} 5.200, -21.621, -10.117 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ p1  PGC 2} -8.144, 18.294, -9.260 
{ o1  PGC 2} -9.638, 18.244, -9.023 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o2  PGC 2} -7.788, 17.399, -10.411 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o3  PGC 2} -7.444, 17.803, -7.991 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o4  PGC 2} -7.757, 19.848, -9.528 
{ c1  PGC 2} -6.772, 20.129, -10.537 
{ c2  PGC 2} -6.304, 21.553, -10.168 
{ o5  PGC 2} -7.174, 22.481, -9.675 
{ c2  PGC 2} P -6.304, 21.553, -10.168 
{ o6  PGC 2} -4.984, 21.847, -10.238 
@subgroup {F6P site} dominant    off
@vectorlist {ht} color= gray  
{ o1   F6P 2 } P -13.626, -9.201, -16.841 
{ o1   F6P 2 } -13.626, -9.201, -16.841 
{ c1   F6P 2 } -12.362, -8.703, -16.439 
{ c2   F6P 2 } -11.618, -8.043, -17.622 
{ o2   F6P 2 } -10.391, -7.537, -17.045 
{ c2   F6P 2 } P -11.618, -8.043, -17.622 
{ c3   F6P 2 } -12.298, -6.949, -18.475 
{ o3   F6P 2 } -12.364, -5.618, -17.960 
{ c3   F6P 2 } P -12.298, -6.949, -18.475 
{ c4   F6P 2 } -11.382, -6.919, -19.704 
{ o4   F6P 2 } -11.951, -6.378, -20.896 
{ c4   F6P 2 } P -11.382, -6.919, -19.704 
{ c5   F6P 2 } -11.131, -8.424, -19.887 
{ c2   F6P 2 } P -11.618, -8.043, -17.622 
{ o5   F6P 2 } -11.283, -9.091, -18.590 
{ c5   F6P 2 } P -11.131, -8.424, -19.887 
{ c6   F6P 2 } -9.752, -8.729, -20.473 
{ o6   F6P 2 } -9.864, -10.053, -21.018 
{ p6   F6P 2 } -8.610, -11.065, -20.899 
{ o61  F6P 2 } -9.007, -12.303, -21.586 
{ p6   F6P 2 } P -8.610, -11.065, -20.899 
{ o62  F6P 2 } -7.448, -10.426, -21.553 
{ p6   F6P 2 } P -8.610, -11.065, -20.899 
{ o63  F6P 2 } -8.429, -11.291, -19.448 
{ c5   F6P 2 } P -11.131, -8.424, -19.887 
{ o5   F6P 2 } -11.283, -9.091, -18.590 
{ oh1   F6P 1 } P 13.626, 9.201, -16.841 
{ o1   F6P 1 } 13.626, 9.201, -16.841 
{ c1   F6P 1 } 12.362, 8.703, -16.439 
{ c2   F6P 1 } 11.618, 8.043, -17.622 
{ o2   F6P 1 } 10.391, 7.537, -17.045 
{ c2   F6P 1 } P 11.618, 8.043, -17.622 
{ c3   F6P 1 } 12.298, 6.949, -18.475 
{ o3   F6P 1 } 12.364, 5.618, -17.960 
{ c3   F6P 1 } P 12.298, 6.949, -18.475 
{ c4   F6P 1 } 11.382, 6.919, -19.704 
{ o4   F6P 1 } 11.951, 6.378, -20.896 
{ c4   F6P 1 } P 11.382, 6.919, -19.704 
{ c5   F6P 1 } 11.131, 8.424, -19.887 
{ c2   F6P 1 } P 11.618, 8.043, -17.622 
{ o5   F6P 1 } 11.283, 9.091, -18.590 
{ c5   F6P 1 } P 11.131, 8.424, -19.887 
{ c6   F6P 1 } 9.752, 8.729, -20.473 
{ o6   F6P 1 } 9.864, 10.053, -21.018 
{ p6   F6P 1 } 8.610, 11.065, -20.899 
{ o61   F6P 1 } 9.007, 12.303, -21.586 
{ p6   F6P 1 } P 8.610, 11.065, -20.899 
{ o62   F6P 1 } 7.448, 10.426, -21.553 
{ p6   F6P 1 } P 8.610, 11.065, -20.899 
{ o63   F6P 1 } 8.429, 11.291, -19.448 
{ c5   F6P 1 } P 11.131, 8.424, -19.887 
{ o5   F6P 1 } 11.283, 9.091, -18.590 
@kinemage 2 
@caption
The allosteric site of phosphofructokinase (PFK) with residues 53-60 not shown.  Residues of R-state Subunits 1 and 2 are pinktint and pink, whereas T state residues of Subunits 1 and 2 are bluetint and skyblue. In the R state, active site-bound F6P is hotpink and allosteric site-bound ADP is yellow. In the T state, allosteric site-bound 2-phosphoglycolate (PGC) is gold. The T state active site, which does not contain F6P, is marked by the position of R state-bound F6P (gray; "F6P site"). The side chains of Glu 161 and Arg 162 are red and cyan.
@viewid {Allosteric site}
@zoom 0.95 
@zslab 284 
@ztran 90
@center 3.029 22.541 -15.274 
@matrix 
-0.79351  0.45801  0.40072  0.44705 -0.00806  0.89447  0.41290  0.88890 -0.19836
@2viewid {closeup}
@2zoom 2.32 
@2zslab 284 
@2center 8.610 11.065 -20.899 
@2matrix 
-0.66815  0.06825  0.74090  0.72516  0.28258  0.62792 -0.16651  0.95680 -0.23830
@perspective 
@group {R-state}    animate   
@subgroup {Dimer} dominant
@vectorlist {ca-ca} color= pinktint    
{ ca  ile   67 } P 7.041, 21.184, -5.782 
{ ca  leu   68 } 9.425, 23.358, -7.921 
{ ca  tyr   69 } 8.196, 21.713, -11.147 
{ ca  thr   70 } 10.481, 20.729, -14.002 
{ ca  ala   71 } 10.768, 21.640, -17.657 
@vectorlist {ca-ca} color= pink     
{ ca  ala   149 } P -2.412, 4.539, -10.288 
{ ca  ile   150 } -4.102, 7.918, -10.677 
{ ca  asp   151 } -2.531, 9.121, -7.443 
{ ca  lys   152 } 0.867, 8.334, -8.903 
{ ca  ile   153 } 0.103, 9.748, -12.286 
{ ca  arg   154 } -1.062, 12.978, -10.685 
{ ca  asp   155 } 2.378, 13.446, -9.070 
{ ca  thr   156 } 4.252, 13.094, -12.338 
{ ca  ala   157 } 1.607, 15.035, -14.222 
{ ca  thr   158 } 1.725, 18.020, -11.824 
{ ca  ser   159 } 5.538, 17.961, -11.955 
{ ca  his   160 } 5.703, 18.445, -15.757 
{ ca  glu   161 } 2.302, 20.172, -16.135 
{ ca  arg   162 } 0.951, 17.473, -18.507 
{ ca  thr   163 } -2.262, 15.807, -19.566 
{ ca  tyr   164 } -2.811, 12.119, -18.991 
{ ca  val   165 } -5.222, 9.769, -20.696 
@vectorlist {ca-ca} color= pink   
{ ca  arg   204 } P -13.783, 22.126, -20.654 
{ ca  leu   205 } -10.042, 21.113, -20.592 
{ ca  lys   206 } -8.872, 24.389, -22.115 
{ ca  arg   207 } -10.998, 26.350, -19.638 
{ ca  gly   208 } -9.456, 24.478, -16.657 
{ ca  his   209 } -5.955, 25.046, -17.970 
{ ca  glu   210 } -6.582, 28.782, -18.325 
{ ca  arg   211 } -8.035, 28.874, -14.820 
{ ca  gly   212 } -4.551, 27.679, -13.749 
{ ca  lys   213 } -4.686, 23.891, -13.341 
{ ca  lys   214 } -1.230, 22.326, -13.534 
{ ca  his   215 } -2.125, 18.806, -14.720 
{ ca  ser   216 } -5.170, 17.286, -16.440 
{ ca  ile   217 } -6.405, 13.643, -15.925 
@subgroup {Glu} dominant    
@balllist {glu-O} radius = 0.3 color=red
{ oe1 glu   161 } 2.991, 24.866, -14.681 
{ oe2 glu   161 } 2.973, 24.368, -16.763 
@vectorlist {sc} color= red     
{ ca  glu   161 } P 2.302, 20.172, -16.135 
{ cb  glu   161 } 2.479, 21.651, -16.380 
{ cg  glu   161 } 3.029, 22.541, -15.274 
{ cd  glu   161 } 2.998, 24.021, -15.560 
{ oe1 glu   161 } 2.991, 24.866, -14.681 
{ cd  glu   161 } P 2.998, 24.021, -15.560 
{ oe2 glu   161 } 2.973, 24.368, -16.763 
@subgroup {Arg} dominant    
@balllist {Arg-N} radius = 0.3 color=blue
{ ne  arg   162 } 5.308, 15.274, -19.679 
{ nh1 arg   162 } 6.293, 13.262, -18.963 
{ nh2 arg   162 } 7.354, 14.608, -20.464 
@vectorlist {sc} color= cyan     
{ ca  arg   162 } P 0.951, 17.473, -18.507 
{ cb  arg   162 } 1.826, 16.200, -18.687 
{ cg  arg   162 } 3.303, 16.367, -18.924 
{ cd  arg   162 } 4.016, 15.074, -19.015 
{ ne  arg   162 } 5.308, 15.274, -19.679 
{ cz  arg   162 } 6.316, 14.398, -19.664 
{ nh1 arg   162 } 6.293, 13.262, -18.963 
{ cz  arg   162 } P 6.316, 14.398, -19.664 
{ nh2 arg   162 } 7.354, 14.608, -20.464 
@vectorlist {H bonds} color= white    
{ o61 F6P} P U 8.628, 12.832, -21.329 
{ nh2 arg   162 } L U 7.733, 14.079, -20.721 
{ nh1 arg   162 } P U 6.800, 12.794, -19.078 
{ o63 F6P} L U 7.922, 11.759, -19.333 
@subgroup {ADP-allo} dominant    
@vectorlist {ht} color= yellow     
{ pb  ADP-allo 1} P -8.236, 18.256, -8.053 
{ pb  ADP-allo 1} -8.236, 18.256, -8.053 
{ o1b ADP-allo 1} -7.491, 17.886, -6.833 
{ pb  ADP-allo 1} P -8.236, 18.256, -8.053 
{ o2b ADP-allo 1} -8.299, 17.230, -9.110 
{ pb  ADP-allo 1} P -8.236, 18.256, -8.053 
{ o3b ADP-allo 1} -9.553, 18.831, -7.688 
{ pa  ADP-allo 1} P -7.758, 20.451, -10.009 
{ o1a ADP-allo 1} -8.626, 21.615, -9.722 
{ pa  ADP-allo 1} P -7.758, 20.451, -10.009 
{ o2a ADP-allo 1} -8.437, 19.676, -11.093 
{ pb  ADP-allo 1} P -8.236, 18.256, -8.053 
{ o3a ADP-allo 1} -7.520, 19.557, -8.695 
{ pa  ADP-allo 1} P -7.758, 20.451, -10.009 
{ o5* ADP-allo 1} -6.284, 20.747, -10.477 
{ c5* ADP-allo 1} -5.266, 19.862, -11.015 
{ c4* ADP-allo 1} -3.943, 20.275, -10.394 
{ o4* ADP-allo 1} -3.781, 21.723, -10.572 
{ c4* ADP-allo 1} P -3.943, 20.275, -10.394 
{ c3* ADP-allo 1} -3.777, 20.025, -8.889 
{ o3* ADP-allo 1} -2.446, 19.902, -8.459 
{ c3* ADP-allo 1} P -3.777, 20.025, -8.889 
{ c2* ADP-allo 1} -4.317, 21.354, -8.315 
{ o2* ADP-allo 1} -3.992, 21.521, -6.952 
{ o4* ADP-allo 1} P -3.781, 21.723, -10.572 
{ c1* ADP-allo 1} -3.659, 22.346, -9.290 
{ n9  ADP-allo 1} -4.314, 23.659, -9.352 
{ c8  ADP-allo 1} -5.627, 23.876, -9.684 
{ n7  ADP-allo 1} -5.993, 25.142, -9.674 
{ c5  ADP-allo 1} P -4.845, 25.795, -9.301 
{ c6  ADP-allo 1} -4.614, 27.180, -9.109 
{ n6  ADP-allo 1} -5.563, 28.100, -9.278 
{ c6  ADP-allo 1} P -4.614, 27.180, -9.109 
{ n1  ADP-allo 1} -3.359, 27.517, -8.732 
{ c2  ADP-allo 1} -2.415, 26.525, -8.574 
{ n3  ADP-allo 1} -2.533, 25.237, -8.724 
{ n9  ADP-allo 1} P -4.314, 23.659, -9.352 
{ c4  ADP-allo 1} -3.800, 24.913, -9.096 
{ pa  ADP-allo 1} P -7.758, 20.451, -10.009 
{ o3a ADP-allo 1} -7.520, 19.557, -8.695 
{ c2* ADP-allo 1} P -4.317, 21.354, -8.315 
{ c1* ADP-allo 1} -3.659, 22.346, -9.290 
{ n7  ADP-allo 1} P -5.993, 25.142, -9.674 
{ c5  ADP-allo 1} -4.845, 25.795, -9.301 
{ c4  ADP-allo 1} -3.800, 24.913, -9.096 
{ n3  ADP-allo 1} P -2.533, 25.237, -8.724 
{ c4  ADP-allo 1} -3.800, 24.913, -9.096 
@balllist color= yellow radius= 0.2
{Mg ADP-allo 1} -8.936, 17.383, -10.956
@subgroup {F6P} dominant      
@balllist {} radius=0.3 color=red
{ o1  F6P 1} P 13.626, 9.201, -16.841 
{ o2  F6P 1} 10.391, 7.537, -17.045 
{ o3  F6P 1} 12.364, 5.618, -17.960 
{ o4  F6P 1} 11.951, 6.378, -20.896 
{ o5  F6P 1} 11.283, 9.091, -18.590 
{ o6  F6P 1} 9.864, 10.053, -21.018 
{ o61 F6P 1} 9.007, 12.303, -21.586 
{ o62 F6P 1} 7.448, 10.426, -21.553 
{ o63 F6P 1} 8.429, 11.291, -19.448 
@balllist {} radius=0.3 color=gold
{ p6  F6P 1} P 8.610, 11.065, -20.899 
@vectorlist {ht} color= hotpink     
{ o1  F6P 1} P 13.626, 9.201, -16.841 
{ o1  F6P 1} 13.626, 9.201, -16.841 
{ c1  F6P 1} 12.362, 8.703, -16.439 
{ c2  F6P 1} 11.618, 8.043, -17.622 
{ o2  F6P 1} 10.391, 7.537, -17.045 
{ c2  F6P 1} P 11.618, 8.043, -17.622 
{ c3  F6P 1} 12.298, 6.949, -18.475 
{ o3  F6P 1} 12.364, 5.618, -17.960 
{ c3  F6P 1} P 12.298, 6.949, -18.475 
{ c4  F6P 1} 11.382, 6.919, -19.704 
{ o4  F6P 1} 11.951, 6.378, -20.896 
{ c4  F6P 1} P 11.382, 6.919, -19.704 
{ c5  F6P 1} 11.131, 8.424, -19.887 
{ c2  F6P 1} P 11.618, 8.043, -17.622 
{ o5  F6P 1} 11.283, 9.091, -18.590 
{ c5  F6P 1} P 11.131, 8.424, -19.887 
{ c6  F6P 1} 9.752, 8.729, -20.473 
{ o6  F6P 1} 9.864, 10.053, -21.018 
{ p6  F6P 1} 8.610, 11.065, -20.899 
{ o61 F6P 1} 9.007, 12.303, -21.586 
{ p6  F6P 1} P 8.610, 11.065, -20.899 
{ o62 F6P 1} 7.448, 10.426, -21.553 
{ p6  F6P 1} P 8.610, 11.065, -20.899 
{ o63 F6P 1} 8.429, 11.291, -19.448 
{ c5  F6P 1} P 11.131, 8.424, -19.887 
{ o5  F6P 1} 11.283, 9.091, -18.590 
@subgroup {R labels} nobutton off master= {Labels}
@labellist {Glu 161} color= red nobutton 
{ Glu 161 } 2.973, 24.368, -16.763 
@labellist {Arg 162} color= cyan nobutton 
{ Arg 162 } 7.354, 14.608, -20.440
@labellist {F6P} color= hotpink nobutton 
{ F6P} 10.391, 7.537, -17.045 
@labellist {R site} color= yellow nobutton
{ ADP-allo} -2.415, 26.525, -8.574 
@group {T-state}    animate   
@subgroup {Dimer} dominant    
@vectorlist {ca-ca} color= bluetint    
{ ca  ile a 67 } P 7.047, 21.460, -4.432 
{ ca  leu a 68 } 9.332, 23.778, -6.435 
{ ca  tyr a 69 } 8.244, 22.182, -9.711 
{ ca  thr a 70 } 10.494, 21.309, -12.617 
{ ca  ala a 71 } 10.393, 22.783, -16.101 
@vectorlist {ca-ca} color= sky    
{ ca  ala b 149 } P -2.119, 4.413, -9.985 
{ ca  ile b 150 } -3.939, 7.744, -10.542 
{ ca  asp b 151 } -2.617, 9.105, -7.296 
{ ca  lys b 152 } 0.927, 8.103, -8.414 
{ ca  ile b 153 } 0.472, 10.096, -11.737 
{ ca  arg b 154 } -0.985, 13.004, -9.687 
{ ca  asp b 155 } 2.550, 13.614, -8.171 
{ ca  thr b 156 } 4.297, 14.117, -11.618 
{ ca  ala b 157 } 4.719, 17.983, -11.251 
{ ca  thr b 158 } 6.055, 18.926, -14.759 
{ ca  ser b 159 } 3.896, 21.395, -16.805 
{ ca  his b 160 } 5.121, 19.885, -20.140 
{ ca  glu b 161 } 3.606, 16.591, -19.196 
{ ca  arg b 162 } 0.126, 17.255, -17.838 
{ ca  thr b 163 } -2.037, 14.927, -20.010 
{ ca  tyr b 164 } -2.471, 11.394, -18.837 
{ ca  val b 165 } -4.705, 8.778, -20.295 
@vectorlist {ca-ca} color= sky    
{ ca  arg b 204 } P -12.360, 20.720, -21.753 
{ ca  leu b 205 } -8.615, 19.998, -21.842 
{ ca  lys b 206 } -8.013, 23.404, -23.417 
{ ca  arg b 207 } -10.246, 25.057, -20.791 
{ ca  gly b 208 } -8.614, 23.239, -17.811 
{ ca  his b 209 } -5.231, 24.199, -19.222 
{ ca  glu b 210 } -6.298, 27.878, -19.483 
{ ca  arg b 211 } -7.146, 28.085, -15.821 
{ ca  gly b 212 } -3.880, 26.423, -14.976 
{ ca  lys b 213 } -5.175, 23.055, -13.768 
{ ca  lys b 214 } -2.187, 20.927, -12.709 
{ ca  his b 215 } -3.288, 17.788, -14.548 
{ ca  ser b 216 } -5.749, 16.682, -17.176 
{ ca  ile b 217 } -6.328, 12.954, -16.437 
@subgroup {Glu} dominant    
@balllist {} color=red radius = 0.3
{ oe2 glu b 161 } 7.084, 13.586, -19.983 
{ oe1 glu b 161 } 7.568, 15.034, -18.408 
@vectorlist {sc} color= red     
{ cd  glu b 161 } P 6.898, 14.637, -19.351 
{ oe2 glu b 161 } 7.084, 13.586, -19.983 
{ cd  glu b 161 } P 6.898, 14.637, -19.351 
{ oe1 glu b 161 } 7.568, 15.034, -18.408 
{ cg  glu b 161 } P 5.712, 15.372, -19.886 
{ cd  glu b 161 } 6.898, 14.637, -19.351 
{ cb  glu b 161 } P 4.562, 15.461, -18.858 
{ cg  glu b 161 } 5.712, 15.372, -19.886 
{ ca  glu b 161 } P 3.606, 16.591, -19.196 
{ cb  glu b 161 } 4.562, 15.461, -18.858 
@subgroup {Arg} dominant    
@balllist {} color=blue radius = 0.3
{ nh2 arg b 162 } -3.160, 22.968, -17.566 
{ nh1 arg b 162 } -0.976, 23.575, -17.082 
{ ne  arg b 162 } P -1.634, 21.356, -17.790 
@vectorlist {sc} color= cyan     
{ cz  arg b 162 } P -1.865, 22.643, -17.432 
{ nh2 arg b 162 } -3.160, 22.968, -17.566 
{ cz  arg b 162 } P -1.865, 22.643, -17.432 
{ nh1 arg b 162 } -0.976, 23.575, -17.082 
{ ne  arg b 162 } P -1.634, 21.356, -17.790 
{ cz  arg b 162 } -1.865, 22.643, -17.432 
{ cd  arg b 162 } P -0.219, 20.977, -17.935 
{ ne  arg b 162 } -1.634, 21.356, -17.790 
{ cg  arg b 162 } P -0.111, 19.701, -18.746 
{ cd  arg b 162 } -0.219, 20.977, -17.935 
{ cb  arg b 162 } P -0.418, 18.658, -17.671 
{ cg  arg b 162 } -0.111, 19.701, -18.746 
{ ca  arg b 162 } P 0.126, 17.255, -17.838 
{ cb  arg b 162 } -0.418, 18.658, -17.671 
@subgroup {PGC} dominant    
@vectorlist {ht} color= gold     
{ p1  h} P -8.144, 18.294, -9.260 
{ p1  PGC 2} -8.144, 18.294, -9.260 
{ o1  PGC 2} -9.638, 18.244, -9.023 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o2  PGC 2} -7.788, 17.399, -10.411 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o3  PGC 2} -7.444, 17.803, -7.991 
{ p1  PGC 2} P -8.144, 18.294, -9.260 
{ o4  PGC 2} -7.757, 19.848, -9.528 
{ c1  PGC 2} -6.772, 20.129, -10.537 
{ c2  PGC 2} -6.304, 21.553, -10.168 
{ o5  PGC 2} -7.174, 22.481, -9.675 
{ c2  PGC 2} P -6.304, 21.553, -10.168 
{ o6  PGC 2} -4.984, 21.847, -10.238 
@subgroup {F6P site} dominant   off    
@balllist {} color=red radius=0.1
{ o61 F6P 1} 9.007, 12.303, -21.586 
{ o62 F6P 1} 7.448, 10.426, -21.553 
{ o63 F6P 1} 8.429, 11.291, -19.448 
@balllist {} color=gold radius=0.1
{ p6  F6P 1} P 8.610, 11.065, -20.899 
@vectorlist {ht} color= gray
{ o1  F6P 1} P 13.626, 9.201, -16.841 
{ o1  F6P 1} 13.626, 9.201, -16.841 
{ c1  F6P 1} 12.362, 8.703, -16.439 
{ c2  F6P 1} 11.618, 8.043, -17.622 
{ o2  F6P 1} 10.391, 7.537, -17.045 
{ c2  F6P 1} P 11.618, 8.043, -17.622 
{ c3  F6P 1} 12.298, 6.949, -18.475 
{ o3  F6P 1} 12.364, 5.618, -17.960 
{ c3  F6P 1} P 12.298, 6.949, -18.475 
{ c4  F6P 1} 11.382, 6.919, -19.704 
{ o4  F6P 1} 11.951, 6.378, -20.896 
{ c4  F6P 1} P 11.382, 6.919, -19.704 
{ c5  F6P 1} 11.131, 8.424, -19.887 
{ c2  F6P 1} P 11.618, 8.043, -17.622 
{ o5  F6P 1} 11.283, 9.091, -18.590 
{ c5  F6P 1} P 11.131, 8.424, -19.887 
{ c6  F6P 1} 9.752, 8.729, -20.473 
{ o6  F6P 1} 9.864, 10.053, -21.018 
{ p6  F6P 1} 8.610, 11.065, -20.899 
{ o61 F6P 1} 9.007, 12.303, -21.586 
{ p6  F6P 1} P 8.610, 11.065, -20.899 
{ o62 F6P 1} 7.448, 10.426, -21.553 
{ p6  F6P 1} P 8.610, 11.065, -20.899 
{ o63 F6P 1} 8.429, 11.291, -19.448 
{ c5  F6P 1} P 11.131, 8.424, -19.887 
{ o5  F6P 1} 11.283, 9.091, -18.590 
@subgroup {T labels} nobutton  off master= {Labels}
@labellist {Glu 161} color= red nobutton
{ Glu 161 } P   5.712, 15.372, -19.886 
@labellist {Arg 162} color= cyan nobutton
{ Arg 162 } P   -1.634, 21.356, -17.790 
@labellist {F6P site} color= gray nobutton
{ F6P site} P   10.391, 7.537, -17.045 
@labellist {T site} color= gold nobutton
{ PGC}  P   -7.174, 22.481, -9.675 



1
	Exercise 13. Phosphofructokinase Allosterism


42
Exercise 14. Glycogen Phosphorylase


46
	Exercise 14. Glycogen Phosphorylase