10mh - Revision history

OCA at 05:33, 9 August 2023

2023-08-09T05:33:23Z

←Older revision		Revision as of 05:33, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='10mh' size='340' side='right'caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='10mh' size='340' side='right'caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10MH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10MH FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SAH:S~~-~~ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_(cytosine~~-5-)-~~methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [https://pdbe.org/10mh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [https://www.ebi.ac.uk/pdbsum/10mh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10mh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [https://pdbe.org/10mh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [https://www.ebi.ac.uk/pdbsum/10mh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10mh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.	+	[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus x pritchett and stillman 1919~~]]	+	[[Category: Haemophilus haemolyticus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alvarez, E]]	+	[[Category: Alvarez E]]
-	[[Category: Brank, A]]	+	[[Category: Brank A]]
-	[[Category: Cheng, X]]	+	[[Category: Cheng X]]
-	[[Category: Christman~~, J K~~]]	+	[[Category: Christman JK]]
-	[[Category: ~~Gara, M O~~]]	+	[[Category: Ford Junior H]]
-	[[Category: Goddard, A]]	+	[[Category: Goddard A]]
-	[[Category: ~~Junior, H Ford~~]]	+	[[Category: Marasco CJ]]
-	[[Category: ~~Marasco, C J~~]]	+	[[Category: Marquez VE]]
-	[[Category: ~~Marquez, V E~~]]	+	[[Category: O'Gara M]]
-	[[Category: Sheikhnejad, G]]	+	[[Category: Sheikhnejad G]]
-	[[Category: Sufrin~~, J R]]~~	+	[[Category: Sufrin JR]]
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Restriction system]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 10:24, 14 July 2021

2021-07-14T10:24:07Z

←Older revision		Revision as of 10:24, 14 July 2021
Line 3:		Line 3:
	<StructureSection load='10mh' size='340' side='right'caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='10mh' size='340' side='right'caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=10MH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10MH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [~~http~~://pdbe.org/10mh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/10mh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=10mh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [https://pdbe.org/10mh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [https://www.ebi.ac.uk/pdbsum/10mh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10mh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.	+	[[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:29, 10 October 2019

2019-10-10T07:29:25Z

←Older revision		Revision as of 07:29, 10 October 2019
Line 1:		Line 1:

	==TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET==		==TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET==
-	<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='10mh' size='340' side='right'caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>		<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 10mh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 10mh" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA methyltransferase 3D structures\|DNA methyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus x pritchett and stillman 1919]]		[[Category: Bacillus x pritchett and stillman 1919]]
		+	[[Category: Large Structures]]
	[[Category: Alvarez, E]]		[[Category: Alvarez, E]]
	[[Category: Brank, A]]		[[Category: Brank, A]]

OCA at 06:52, 3 January 2018

2018-01-03T06:52:38Z

←Older revision		Revision as of 06:52, 3 January 2018
Line 1:		Line 1:
		+
	==TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET==		==TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET==
	<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://pdbe.org/10mh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://pdbe.org/10mh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=10mh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/0m/10mh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/0m/10mh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 10mh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 10mh" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[HhaI DNA methyltransferase\|HhaI DNA methyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:02, 7 February 2016

2016-02-07T14:02:47Z

←Older revision		Revision as of 14:02, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=10mh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:06, 9 September 2015

2015-09-09T16:06:11Z

←Older revision		Revision as of 16:06, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Haemophilus_haemolyticus Haemophilus haemolyticus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Deleted_entry Deleted entry~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://pdbe.org/10mh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 10mh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deleted entry]]~~	+	[[Category: Bacillus x pritchett and stillman 1919]]
-	~~[[Category: Haemophilus haemolyticus~~]]	+
	[[Category: Alvarez, E]]		[[Category: Alvarez, E]]
	[[Category: Brank, A]]		[[Category: Brank, A]]

OCA at 10:24, 22 December 2014

2014-12-22T10:24:38Z

←Older revision		Revision as of 10:24, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>		<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Haemophilus haemolyticus]]		[[Category: Haemophilus haemolyticus]]
-	[[Category: Alvarez, E.]]	+	[[Category: Alvarez, E]]
-	[[Category: Brank, A.]]	+	[[Category: Brank, A]]
-	[[Category: Cheng, X.]]	+	[[Category: Cheng, X]]
-	[[Category: Christman, J K.]]	+	[[Category: Christman, J K]]
-	[[Category: Gara, M O.]]	+	[[Category: Gara, M O]]
-	[[Category: Goddard, A.]]	+	[[Category: Goddard, A]]
-	[[Category: Junior, H Ford.]]	+	[[Category: Junior, H Ford]]
-	[[Category: Marasco, C J.]]	+	[[Category: Marasco, C J]]
-	[[Category: Marquez, V E.]]	+	[[Category: Marquez, V E]]
-	[[Category: Sheikhnejad, G.]]	+	[[Category: Sheikhnejad, G]]
-	[[Category: Sufrin, J R.]]	+	[[Category: Sufrin, J R]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Restriction system]]		[[Category: Restriction system]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Transferase-dna complex]]		[[Category: Transferase-dna complex]]

OCA at 08:37, 23 July 2014

2014-07-23T08:37:44Z

←Older revision		Revision as of 08:37, 23 July 2014
Line 1:		Line 1:
-	[[~~Image:~~10mh.~~png~~\|~~left~~\|200px]]	+	==TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET==
		+	<StructureSection load='10mh' size='340' side='right' caption='[[10mh]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10MH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=5NC:5-AZA-CYTIDINE-5MONOPHOSPHATE'>5NC</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=10mh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10mh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=10mh RCSB], [http://www.ebi.ac.uk/pdbsum/10mh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/0m/10mh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A key step in the predicted mechanism of enzymatic transfer of methyl groups from S-adenosyl-l-methionine (AdoMet) to cytosine residues in DNA is the transient formation of a dihydrocytosine intermediate covalently linked to cysteine in the active site of a DNA (cytosine C5)-methyltransferase (DNA C5-MTase). Crystallographic analysis of complexes formed by HhaI methyltransferase (M.HhaI), AdoMet and a target oligodeoxyribonucleotide containing 5-fluorocytosine confirmed the existence of this dihydrocytosine intermediate. Based on the premise that 5,6-dihydro-5-azacytosine (DZCyt), a cytosine analog with an sp3-hybridized carbon (CH2) at position 6 and an NH group at position 5, could mimic the non-aromatic character of the cytosine ring in this transition state, we synthesized a series of synthetic substrates for DNA C5-MTase containing DZCyt. Substitution of DZCyt for target cytosines in C-G dinucleotides of single-stranded or double-stranded oligodeoxyribonucleotide substrates led to complete inhibition of methylation by murine DNA C5-MTase. Substitution of DZCyt for the target cytosine in G-C-G-C sites in double-stranded oligodeoxyribonucleotides had a similar effect on methylation by M. HhaI. Oligodeoxyribonucleotides containing DZCyt formed a tight but reversible complex with M.HhaI, and were consistently more potent as inhibitors of DNA methylation than oligodeoxyribonucleotides identical in sequence containing 5-fluorocytosine. Crystallographic analysis of a ternary complex involving M.HhaI, S-adenosyl-l-homocysteine and a double-stranded 13-mer oligodeoxyribonucleotide containing DZCyt at the target position showed that the analog is flipped out of the DNA helix in the same manner as cytosine, 5-methylcytosine, and 5-fluorocytosine. However, no formation of a covalent bond was detected between the sulfur atom of the catalytic site nucleophile, cysteine 81, and the pyrimidine C6 carbon. These results indicate that DZCyt can occupy the active site of M.HhaI as a transition state mimic and, because of the high degree of affinity of its interaction with the enzyme, it can act as a potent inhibitor of methylation.

-	~~{{STRUCTURE_10mh\| PDB=10mh \| SCENE= }}~~	+	Mechanism of inhibition of DNA (cytosine C5)-methyltransferases by oligodeoxyribonucleotides containing 5,6-dihydro-5-azacytosine.,Sheikhnejad G, Brank A, Christman JK, Goddard A, Alvarez E, Ford H Jr, Marquez VE, Marasco CJ, Sufrin JR, O'gara M, Cheng X J Mol Biol. 1999 Feb 5;285(5):2021-34. PMID:9925782<ref>PMID:9925782</ref>

-	~~===TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5~~,~~6-DIHYDRO-5-AZACYTOSINE AT THE TARGET===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_9925782}}~~	+	==See Also==
-		+	*[[HhaI DNA methyltransferase\|HhaI DNA methyltransferase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~10mh~~]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:009925782<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Haemophilus haemolyticus]]		[[Category: Haemophilus haemolyticus]]

OCA: Protected "10mh" [edit=sysop:move=sysop]

2012-10-21T12:23:53Z

Protected "10mh" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:23, 21 October 2012

OCA at 12:23, 21 October 2012

2012-10-21T12:23:50Z

←Older revision		Revision as of 12:23, 21 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:10mh.png\|left\|200px]]		[[Image:10mh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_10mh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_10mh\| PDB=10mh \| SCENE= }}		{{STRUCTURE_10mh\| PDB=10mh \| SCENE= }}

	===TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET===		===TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH ADOHCY AND HEMIMETHYLATED DNA CONTAINING 5,6-DIHYDRO-5-AZACYTOSINE AT THE TARGET===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9925782}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9925782 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9925782}}		{{ABSTRACT_PUBMED_9925782}}

	==About this Structure==		==About this Structure==
-	~~10MH~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA].	+	[[10mh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10MH OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9925782~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009925782</ref><references group="xtra"/>
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Haemophilus haemolyticus]]		[[Category: Haemophilus haemolyticus]]
Line 40:		Line 28:
	[[Category: Restriction system]]		[[Category: Restriction system]]
	[[Category: Transferase]]		[[Category: Transferase]]
-		+	[[Category: Transferase-dna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 14:17:59 2009''~~	+