1a59 - Revision history

OCA at 06:27, 7 February 2024

2024-02-07T06:27:44Z

←Older revision		Revision as of 06:27, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='1a59' size='340' side='right'caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='1a59' size='340' side='right'caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Abds2 Abds2~~]. The September 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Antarctic_bacterium_DS2-3R Antarctic bacterium DS2-3R]. The September 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [https://pdbe.org/1a59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [https://www.ebi.ac.uk/pdbsum/1a59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a59 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [https://pdbe.org/1a59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [https://www.ebi.ac.uk/pdbsum/1a59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a59 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PRPC_ABDS2 PRPC_ABDS2] Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield 2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity.<ref>PMID:9310359</ref> <ref>PMID:9579066</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a59 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a59 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: The structural basis of adaptation of enzymes to low temperature is poorly understood. Dimeric citrate synthase has been used as a model enzyme to study the structural basis of thermostability, the structure of the enzyme from organisms living in habitats at 55 degrees C and 100 degrees C having previously been determined. Here the study is extended to include a citrate synthase from an Antarctic bacterium, allowing us to explore the structural basis of cold activity and thermostability across the whole temperature range over which life is known to exit. RESULTS: We report here the first crystal structure of a cold-active enzyme, citrate synthase, isolated from an Antarctic bacterium, at a resolution of 2.09 A. In comparison with the same enzyme from a hyperthermophilic host, the cold-active enzyme has a much more accessible active site, an unusual electrostatic potential distribution and an increased relative flexibility of the small domain compared to the large domain. Several other features of the cold-active enzyme were also identified: reduced subunit interface interactions with no intersubunit ion-pair networks; loops of increased length carrying more charge and fewer proline residues; an increase in solvent-exposed hydrophobic residues; and an increase in intramolecular ion pairs. CONCLUSIONS: Enzymes from organisms living at the temperature extremes of life need to avoid hot or cold denaturation yet maintain sufficient structural integrity to allow catalytic efficiency. For hyperthermophiles, thermal denaturation of the citrate synthase dimer appears to be resisted by complex networks of ion pairs at the dimer interface, a feature common to other hyperthermophilic proteins. For the cold-active citrate synthase, cold denaturation appears to be resisted by an increase in intramolecular ion pairs compared to the hyperthermophilic enzyme. Catalytic efficiency of the cold-active enzyme appears to be achieved by a more accessible active site and by an increase in the relative flexibility of the small domain compared to the large domain.
-
-	Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium.,Russell RJ, Gerike U, Danson MJ, Hough DW, Taylor GL Structure. 1998 Mar 15;6(3):351-61. PMID:9551556<ref>PMID:9551556</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Abds2~~]]	+	[[Category: Antarctic bacterium DS2-3R]]
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Danson~~, M J~~]]	+	[[Category: Danson MJ]]
-	[[Category: Gerike, U]]	+	[[Category: Gerike U]]
-	[[Category: Hough~~, D W~~]]	+	[[Category: Hough DW]]
-	[[Category: Russell~~, R J.M~~]]	+	[[Category: Russell RJM]]
-	[[Category: Taylor~~, G L]]~~	+	[[Category: Taylor GL]]
-	~~[[Category: Cold-activity~~]]	+

OCA at 09:42, 26 May 2021

2021-05-26T09:42:19Z

←Older revision		Revision as of 09:42, 26 May 2021
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	<StructureSection load='1a59' size='340' side='right'caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='1a59' size='340' side='right'caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1A59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [~~http~~://pdbe.org/1a59 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1a59 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1a59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [https://pdbe.org/1a59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [https://www.ebi.ac.uk/pdbsum/1a59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a59 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:33, 21 August 2019

2019-08-21T07:33:36Z

←Older revision		Revision as of 07:33, 21 August 2019
Line 1:		Line 1:

	==COLD-ACTIVE CITRATE SYNTHASE==		==COLD-ACTIVE CITRATE SYNTHASE==
-	<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>	+	<StructureSection load='1a59' size='340' side='right'caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a59_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a59_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Citrate Synthase 3D structures\|Citrate Synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	[[Category: Abds2]]		[[Category: Abds2]]
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Danson, M J]]		[[Category: Danson, M J]]

OCA at 10:36, 15 November 2017

2017-11-15T10:36:38Z

←Older revision		Revision as of 10:36, 15 November 2017
Line 1:		Line 1:
		+
	==COLD-ACTIVE CITRATE SYNTHASE==		==COLD-ACTIVE CITRATE SYNTHASE==
	<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://pdbe.org/1a59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://pdbe.org/1a59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a59 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Citrate Synthase\|Citrate Synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:05, 10 February 2016

2016-02-10T00:05:37Z

←Older revision		Revision as of 00:05, 10 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a59 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:46, 9 September 2015

2015-09-09T15:46:41Z

←Older revision		Revision as of 15:46, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r~~]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Abds2 Abds2]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://pdbe.org/1a59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1a59" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Antarctic bacterium ds2-3r~~]]	+	[[Category: Abds2]]
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]

OCA at 11:02, 22 December 2014

2014-12-22T11:02:24Z

←Older revision		Revision as of 11:02, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Danson, M J.]]	+	[[Category: Danson, M J]]
-	[[Category: Gerike, U.]]	+	[[Category: Gerike, U]]
-	[[Category: Hough, D W.]]	+	[[Category: Hough, D W]]
-	[[Category: Russell, R J.M.]]	+	[[Category: Russell, R J.M]]
-	[[Category: Taylor, G L.]]	+	[[Category: Taylor, G L]]
	[[Category: Cold-activity]]		[[Category: Cold-activity]]

OCA at 08:28, 23 July 2014

2014-07-23T08:28:36Z

←Older revision		Revision as of 08:28, 23 July 2014
Line 1:		Line 1:
-	[[~~Image~~:1a59.~~png\|left~~\|200px]]	+	==COLD-ACTIVE CITRATE SYNTHASE==
		+	<StructureSection load='1a59' size='340' side='right' caption='[[1a59]], [[Resolution\|resolution]] 2.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A59 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a59 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a59 RCSB], [http://www.ebi.ac.uk/pdbsum/1a59 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a59_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The structural basis of adaptation of enzymes to low temperature is poorly understood. Dimeric citrate synthase has been used as a model enzyme to study the structural basis of thermostability, the structure of the enzyme from organisms living in habitats at 55 degrees C and 100 degrees C having previously been determined. Here the study is extended to include a citrate synthase from an Antarctic bacterium, allowing us to explore the structural basis of cold activity and thermostability across the whole temperature range over which life is known to exit. RESULTS: We report here the first crystal structure of a cold-active enzyme, citrate synthase, isolated from an Antarctic bacterium, at a resolution of 2.09 A. In comparison with the same enzyme from a hyperthermophilic host, the cold-active enzyme has a much more accessible active site, an unusual electrostatic potential distribution and an increased relative flexibility of the small domain compared to the large domain. Several other features of the cold-active enzyme were also identified: reduced subunit interface interactions with no intersubunit ion-pair networks; loops of increased length carrying more charge and fewer proline residues; an increase in solvent-exposed hydrophobic residues; and an increase in intramolecular ion pairs. CONCLUSIONS: Enzymes from organisms living at the temperature extremes of life need to avoid hot or cold denaturation yet maintain sufficient structural integrity to allow catalytic efficiency. For hyperthermophiles, thermal denaturation of the citrate synthase dimer appears to be resisted by complex networks of ion pairs at the dimer interface, a feature common to other hyperthermophilic proteins. For the cold-active citrate synthase, cold denaturation appears to be resisted by an increase in intramolecular ion pairs compared to the hyperthermophilic enzyme. Catalytic efficiency of the cold-active enzyme appears to be achieved by a more accessible active site and by an increase in the relative flexibility of the small domain compared to the large domain.

-	~~{{STRUCTURE_1a59\| PDB=1a59 \| SCENE= }}~~	+	Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium.,Russell RJ, Gerike U, Danson MJ, Hough DW, Taylor GL Structure. 1998 Mar 15;6(3):351-61. PMID:9551556<ref>PMID:9551556</ref>

-	~~===COLD-ACTIVE CITRATE SYNTHASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9551556}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1a59]] is~~ a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the ~~Month] feature on ''Citrate Synthase'' by David~~ S. ~~Goodsell is [http://dx~~.~~doi.org~~/~~10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA].~~	+

	==See Also==		==See Also==
	*[[Citrate Synthase\|Citrate Synthase]]		*[[Citrate Synthase\|Citrate Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009551556<~~/~~ref~~><~~ref group="xtra">PMID:014997520</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Antarctic bacterium ds2-3r]]		[[Category: Antarctic bacterium ds2-3r]]
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]

OCA at 15:36, 20 October 2012

2012-10-20T15:36:52Z

←Older revision		Revision as of 15:36, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1a59]] is a 1 chain structure ~~of [[Citrate Synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/~~Bacteria Bacteria~~]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA].	+	[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_ds2-3r Antarctic bacterium ds2-3r]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA].

	==See Also==		==See Also==
Line 15:		Line 15:
	==Reference==		==Reference==
	<ref group="xtra">PMID:009551556</ref><ref group="xtra">PMID:014997520</ref><references group="xtra"/>		<ref group="xtra">PMID:009551556</ref><ref group="xtra">PMID:014997520</ref><references group="xtra"/>
-	[[Category: ~~Bacteria~~]]	+	[[Category: Antarctic bacterium ds2-3r]]
	[[Category: Citrate Synthase]]		[[Category: Citrate Synthase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]

OCA at 18:56, 26 July 2012

2012-07-26T18:56:07Z

←Older revision		Revision as of 18:56, 26 July 2012
Line 1:		Line 1:
	[[Image:1a59.png\|left\|200px]]		[[Image:1a59.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1a59", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1a59\| PDB=1a59 \| SCENE= }}		{{STRUCTURE_1a59\| PDB=1a59 \| SCENE= }}

	===COLD-ACTIVE CITRATE SYNTHASE===		===COLD-ACTIVE CITRATE SYNTHASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9551556}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9551556 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9551556}}		{{ABSTRACT_PUBMED_9551556}}

	==About this Structure==		==About this Structure==
-	[[1a59]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA].	+	[[1a59]] is a 1 chain structure of [[Citrate Synthase]] with sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. The September 2007 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A59 OCA].
		+
		+	==See Also==
		+	*[[Citrate Synthase\|Citrate Synthase]]

	==Reference==		==Reference==