1ae1 - Revision history

OCA at 06:29, 7 February 2024

2024-02-07T06:29:55Z

←Older revision		Revision as of 06:29, 7 February 2024
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	<StructureSection load='1ae1' size='340' side='right'caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ae1' size='340' side='right'caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAP:NADP+NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [https://pdbe.org/1ae1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [https://pdbe.org/1ae1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST]] Catalyzes the stereospecific reduction of tropinone to tropine.	+	[https://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST] Catalyzes the stereospecific reduction of tropinone to tropine.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A pair of tropinone reductases (TRs) share 64% of the same amino acid residues and belong to the short-chain dehydrogenase/reductase family. In the synthesis of tropane alkaloids in several medicinal plants, the TRs reduce a carbonyl group of an alkaloid intermediate, tropinone, to hydroxy groups with different diastereomeric configurations. To clarify the structural basis for their different reaction stereospecificities, we determined the crystal structures of the two enzymes at 2.4- and 2.3-A resolutions. The overall folding of the two enzymes was almost identical. The conservation was not confined within the core domains that are conserved within the protein family but extended outside the core domain where each family member has its characteristic structure. The binding sites for the cofactor and the positions of the active site residues were well conserved between the two TRs. The substrate binding site was composed mostly of hydrophobic amino acids in both TRs, but the presence of different charged residues conferred different electrostatic environments on the two enzymes. A modeling study indicated that these charged residues play a major role in controlling the binding orientation of tropinone within the substrate binding site, thereby determining the stereospecificity of the reaction product. The results obtained herein raise the possibility that in certain cases different stereospecificities can be acquired in enzymes by changing a few amino acid residues within substrate binding sites.
-
-	Crystal structures of two tropinone reductases: different reaction stereospecificities in the same protein fold.,Nakajima K, Yamashita A, Akama H, Nakatsu T, Kato H, Hashimoto T, Oda J, Yamada Y Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4876-81. PMID:9560196<ref>PMID:9560196</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ae1" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Datura stramonium]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Tropinone reductase II]]~~	+	[[Category: Akama H]]
-	[[Category: Akama, H]]	+	[[Category: Hashimoto T]]
-	[[Category: Hashimoto, T]]	+	[[Category: Kato H]]
-	[[Category: Kato, H]]	+	[[Category: Nakajima K]]
-	[[Category: Nakajima, K]]	+	[[Category: Nakatsu T]]
-	[[Category: Nakatsu, T]]	+	[[Category: Oda J]]
-	[[Category: Oda, J]]	+	[[Category: Yamada Y]]
-	[[Category: Yamada, Y]]	+	[[Category: Yamashita A]]
-	[[Category: Yamashita, A]]	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reduction of tropinone to tropine]]~~	+
-	~~[[Category: Short-chain dehydrogenase]]~~	+
-	~~[[Category: Tropane alkaloid biosynthesis~~]]	+

OCA at 10:19, 17 February 2021

2021-02-17T10:19:19Z

←Older revision		Revision as of 10:19, 17 February 2021
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	<StructureSection load='1ae1' size='340' side='right'caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ae1' size='340' side='right'caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [~~http~~://pdbe.org/1ae1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ae1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [https://pdbe.org/1ae1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ae1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST]] Catalyzes the stereospecific reduction of tropinone to tropine.	+	[[https://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST]] Catalyzes the stereospecific reduction of tropinone to tropine.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1ae1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ae1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[InhA\|InhA]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:06, 10 May 2019

2019-05-10T14:06:43Z

←Older revision		Revision as of 14:06, 10 May 2019
Line 1:		Line 1:

	==TROPINONE REDUCTASE-I COMPLEX WITH NADP==		==TROPINONE REDUCTASE-I COMPLEX WITH NADP==
-	<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1ae1' size='340' side='right'caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Tropinone reductase II]]		[[Category: Tropinone reductase II]]
	[[Category: Akama, H]]		[[Category: Akama, H]]

OCA at 09:15, 9 August 2017

2017-08-09T09:15:03Z

←Older revision		Revision as of 09:15, 9 August 2017
Line 1:		Line 1:
		+
	==TROPINONE REDUCTASE-I COMPLEX WITH NADP==		==TROPINONE REDUCTASE-I COMPLEX WITH NADP==
	<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Common_thornapple Common thornapple]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://pdbe.org/1ae1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://pdbe.org/1ae1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ae1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ae1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Common thornapple]]
	[[Category: Tropinone reductase II]]		[[Category: Tropinone reductase II]]
	[[Category: Akama, H]]		[[Category: Akama, H]]

OCA at 09:45, 11 September 2015

2015-09-11T09:45:59Z

←Older revision		Revision as of 09:45, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Datura_stramonium Datura stramonium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_thornapple Common thornapple]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://pdbe.org/1ae1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ae1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Datura stramonium~~]]	+	[[Category: Common thornapple]]
	[[Category: Tropinone reductase II]]		[[Category: Tropinone reductase II]]
	[[Category: Akama, H]]		[[Category: Akama, H]]

OCA at 14:41, 25 December 2014

2014-12-25T14:41:47Z

←Older revision		Revision as of 14:41, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST]] Catalyzes the stereospecific reduction of tropinone to tropine.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:29, 22 December 2014

2014-12-22T11:29:37Z

←Older revision		Revision as of 11:29, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Datura stramonium]]		[[Category: Datura stramonium]]
	[[Category: Tropinone reductase II]]		[[Category: Tropinone reductase II]]
-	[[Category: Akama, H.]]	+	[[Category: Akama, H]]
-	[[Category: Hashimoto, T.]]	+	[[Category: Hashimoto, T]]
-	[[Category: Kato, H.]]	+	[[Category: Kato, H]]
-	[[Category: Nakajima, K.]]	+	[[Category: Nakajima, K]]
-	[[Category: Nakatsu, T.]]	+	[[Category: Nakatsu, T]]
-	[[Category: Oda, J.]]	+	[[Category: Oda, J]]
-	[[Category: Yamada, Y.]]	+	[[Category: Yamada, Y]]
-	[[Category: Yamashita, A.]]	+	[[Category: Yamashita, A]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Reduction of tropinone to tropine]]		[[Category: Reduction of tropinone to tropine]]
	[[Category: Short-chain dehydrogenase]]		[[Category: Short-chain dehydrogenase]]
	[[Category: Tropane alkaloid biosynthesis]]		[[Category: Tropane alkaloid biosynthesis]]

OCA at 17:18, 29 September 2014

2014-09-29T17:18:22Z

←Older revision		Revision as of 17:18, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1ae1\| PDB~~=1ae1 \| ~~SCENE~~= }}	+	==TROPINONE REDUCTASE-I COMPLEX WITH NADP==
-	===~~TROPINONE REDUCTASE~~-~~I COMPLEX WITH~~ NADP===	+	<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_9560196}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Datura_stramonium Datura stramonium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A pair of tropinone reductases (TRs) share 64% of the same amino acid residues and belong to the short-chain dehydrogenase/reductase family. In the synthesis of tropane alkaloids in several medicinal plants, the TRs reduce a carbonyl group of an alkaloid intermediate, tropinone, to hydroxy groups with different diastereomeric configurations. To clarify the structural basis for their different reaction stereospecificities, we determined the crystal structures of the two enzymes at 2.4- and 2.3-A resolutions. The overall folding of the two enzymes was almost identical. The conservation was not confined within the core domains that are conserved within the protein family but extended outside the core domain where each family member has its characteristic structure. The binding sites for the cofactor and the positions of the active site residues were well conserved between the two TRs. The substrate binding site was composed mostly of hydrophobic amino acids in both TRs, but the presence of different charged residues conferred different electrostatic environments on the two enzymes. A modeling study indicated that these charged residues play a major role in controlling the binding orientation of tropinone within the substrate binding site, thereby determining the stereospecificity of the reaction product. The results obtained herein raise the possibility that in certain cases different stereospecificities can be acquired in enzymes by changing a few amino acid residues within substrate binding sites.

-	~~==About this Structure==~~	+	Crystal structures of two tropinone reductases: different reaction stereospecificities in the same protein fold.,Nakajima K, Yamashita A, Akama H, Nakatsu T, Kato H, Hashimoto T, Oda J, Yamada Y Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4876-81. PMID:9560196<ref>PMID:9560196</ref>
-	~~[[1ae1]] is a 2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Datura_stramonium Datura stramonium]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1AE1 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[InhA\|InhA]]		*[[InhA\|InhA]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009560196<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Datura stramonium]]		[[Category: Datura stramonium]]
	[[Category: Tropinone reductase II]]		[[Category: Tropinone reductase II]]

OCA at 10:49, 11 March 2013

2013-03-11T10:49:00Z

←Older revision		Revision as of 10:49, 11 March 2013
Line 1:		Line 1:
-	[[Image:1ae1.png\|left\|200px]]
-
	{{STRUCTURE_1ae1\| PDB=1ae1 \| SCENE= }}		{{STRUCTURE_1ae1\| PDB=1ae1 \| SCENE= }}
-
	===TROPINONE REDUCTASE-I COMPLEX WITH NADP===		===TROPINONE REDUCTASE-I COMPLEX WITH NADP===
-
	{{ABSTRACT_PUBMED_9560196}}		{{ABSTRACT_PUBMED_9560196}}

OCA: Protected "1ae1" [edit=sysop:move=sysop]

2012-10-21T11:47:37Z

Protected "1ae1" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 21 October 2012