1bcg - Revision history

OCA at 15:31, 13 March 2024

2024-03-13T15:31:45Z

←Older revision		Revision as of 15:31, 13 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [https://pdbe.org/1bcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [https://www.ebi.ac.uk/pdbsum/1bcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [https://pdbe.org/1bcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [https://www.ebi.ac.uk/pdbsum/1bcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SIXE_HOTJU SIXE_HOTJU]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.	+	[https://www.uniprot.org/uniprot/SIXE_HOTJU SIXE_HOTJU] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bcg ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bcg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Scorpion neurotoxins, which bind and modulate sodium channels, have been divided into two groups, the alpha and beta toxins, according to their activities. The beta-toxin class includes the groups of excitatory and depressant toxins, which differ in their mode of action and are highly specific against insects. The three-dimensional structures of several alpha and beta toxins have been determined at high resolution, but no detailed 3D structure of an excitatory toxin has been presented so far. RESULTS: The crystal structure of an anti-insect excitatory toxin from the scorpion Buthotus judaicus, Bj-xtrIT, has been determined at 2.1 A resolution and refined to an R factor of 0.209. The first 59 residues form a closely packed module, structurally similar to the conserved alpha and beta toxins ('long toxins') affecting sodium channels. The last 17 residues form a C-terminal extension not previously seen in scorpion toxins. It comprises a short alpha helix anchored to the N-terminal module by a disulfide bridge and is followed by a highly mobile stretch of seven residues, of which only four are seen in the electron-density map. This mobile peptide covers part of a conserved hydrophobic surface that is thought to be essential for interaction with the channel in several long toxins. CONCLUSIONS: Replacement of the last seven residues by a single glycine abolishes the activity of Bj-xtrIT, strongly suggesting that these residues are intimately involved in the interaction with the channel. Taken together with the partial shielding of the conserved hydrophobic surface and the proximity of the C terminus to an adjacent surface rich in charged residues, it seems likely that the bioactive surface of Bj-xtrIT is formed by residues surrounding the C terminus. The 3D structure and a recently developed expression system for Bj-xtrIT pave the way for identifying the structural determinants involved in the bioactivity and anti-insect specificity of excitatory toxins.
-
-	An excitatory scorpion toxin with a distinctive feature: an additional alpha helix at the C terminus and its implications for interaction with insect sodium channels.,Oren DA, Froy O, Amit E, Kleinberger-Doron N, Gurevitz M, Shaanan B Structure. 1998 Sep 15;6(9):1095-103. PMID:9753689<ref>PMID:9753689</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1bcg" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Hottentotta judaicus]]		[[Category: Hottentotta judaicus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amit, E]]	+	[[Category: Amit E]]
-	[[Category: Froy, O]]	+	[[Category: Froy O]]
-	[[Category: Gurevitz, M]]	+	[[Category: Gurevitz M]]
-	[[Category: Kleinberger-Doron, N]]	+	[[Category: Kleinberger-Doron N]]
-	[[Category: Oren, D]]	+	[[Category: Oren D]]
-	[[Category: Shaanan, B]]	+	[[Category: Shaanan B]]
-	~~[[Category: Excitatory neurotoxin~~]]	+

OCA at 15:06, 2 June 2021

2021-06-02T15:06:24Z

←Older revision		Revision as of 15:06, 2 June 2021
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	<StructureSection load='1bcg' size='340' side='right'caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bcg' size='340' side='right'caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [~~http~~://pdbe.org/1bcg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bcg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcg ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [https://pdbe.org/1bcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [https://www.ebi.ac.uk/pdbsum/1bcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SIXE_HOTJU SIXE_HOTJU]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.	+	[[https://www.uniprot.org/uniprot/SIXE_HOTJU SIXE_HOTJU]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:38, 28 August 2019

2019-08-28T16:38:35Z

←Older revision		Revision as of 16:38, 28 August 2019
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	==SCORPION TOXIN BJXTR-IT==		==SCORPION TOXIN BJXTR-IT==
-	<StructureSection load='1bcg' size='340' side='right' caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1bcg' size='340' side='right'caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bcg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bcg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Hottentotta judaicus]]		[[Category: Hottentotta judaicus]]
		+	[[Category: Large Structures]]
	[[Category: Amit, E]]		[[Category: Amit, E]]
	[[Category: Froy, O]]		[[Category: Froy, O]]

OCA at 11:04, 22 November 2017

2017-11-22T11:04:32Z

←Older revision		Revision as of 11:04, 22 November 2017
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		+
	==SCORPION TOXIN BJXTR-IT==		==SCORPION TOXIN BJXTR-IT==
	<StructureSection load='1bcg' size='340' side='right' caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1bcg' size='340' side='right' caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://pdbe.org/1bcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://pdbe.org/1bcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:02, 9 February 2016

2016-02-09T08:02:55Z

←Older revision		Revision as of 08:02, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bcg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:15, 10 September 2015

2015-09-10T20:15:05Z

←Older revision		Revision as of 20:15, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://pdbe.org/1bcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~SIXE_BUTJU SIXE_BUTJU~~]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.	+	[[http://www.uniprot.org/uniprot/SIXE_HOTJU SIXE_HOTJU]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bcg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:33, 25 December 2014

2014-12-25T05:33:50Z

←Older revision		Revision as of 05:33, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SIXE_BUTJU SIXE_BUTJU]] Excitatory insect toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:59, 22 December 2014

2014-12-22T09:59:28Z

←Older revision		Revision as of 09:59, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Hottentotta judaicus]]		[[Category: Hottentotta judaicus]]
-	[[Category: Amit, E.]]	+	[[Category: Amit, E]]
-	[[Category: Froy, O.]]	+	[[Category: Froy, O]]
-	[[Category: Gurevitz, M.]]	+	[[Category: Gurevitz, M]]
-	[[Category: Kleinberger-Doron, N.]]	+	[[Category: Kleinberger-Doron, N]]
-	[[Category: Oren, D.]]	+	[[Category: Oren, D]]
-	[[Category: Shaanan, B.]]	+	[[Category: Shaanan, B]]
	[[Category: Excitatory neurotoxin]]		[[Category: Excitatory neurotoxin]]

OCA at 03:07, 7 August 2014

2014-08-07T03:07:33Z

←Older revision		Revision as of 03:07, 7 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1bcg.~~png\|left~~\|200px]]	+	==SCORPION TOXIN BJXTR-IT==
		+	<StructureSection load='1bcg' size='340' side='right' caption='[[1bcg]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bcg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hottentotta_judaicus Hottentotta judaicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BCG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcg RCSB], [http://www.ebi.ac.uk/pdbsum/1bcg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bcg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Scorpion neurotoxins, which bind and modulate sodium channels, have been divided into two groups, the alpha and beta toxins, according to their activities. The beta-toxin class includes the groups of excitatory and depressant toxins, which differ in their mode of action and are highly specific against insects. The three-dimensional structures of several alpha and beta toxins have been determined at high resolution, but no detailed 3D structure of an excitatory toxin has been presented so far. RESULTS: The crystal structure of an anti-insect excitatory toxin from the scorpion Buthotus judaicus, Bj-xtrIT, has been determined at 2.1 A resolution and refined to an R factor of 0.209. The first 59 residues form a closely packed module, structurally similar to the conserved alpha and beta toxins ('long toxins') affecting sodium channels. The last 17 residues form a C-terminal extension not previously seen in scorpion toxins. It comprises a short alpha helix anchored to the N-terminal module by a disulfide bridge and is followed by a highly mobile stretch of seven residues, of which only four are seen in the electron-density map. This mobile peptide covers part of a conserved hydrophobic surface that is thought to be essential for interaction with the channel in several long toxins. CONCLUSIONS: Replacement of the last seven residues by a single glycine abolishes the activity of Bj-xtrIT, strongly suggesting that these residues are intimately involved in the interaction with the channel. Taken together with the partial shielding of the conserved hydrophobic surface and the proximity of the C terminus to an adjacent surface rich in charged residues, it seems likely that the bioactive surface of Bj-xtrIT is formed by residues surrounding the C terminus. The 3D structure and a recently developed expression system for Bj-xtrIT pave the way for identifying the structural determinants involved in the bioactivity and anti-insect specificity of excitatory toxins.

-	~~{{STRUCTURE_1bcg\| PDB=1bcg \| SCENE= }}~~	+	An excitatory scorpion toxin with a distinctive feature: an additional alpha helix at the C terminus and its implications for interaction with insect sodium channels.,Oren DA, Froy O, Amit E, Kleinberger-Doron N, Gurevitz M, Shaanan B Structure. 1998 Sep 15;6(9):1095-103. PMID:9753689<ref>PMID:9753689</ref>

-	~~===SCORPION TOXIN BJXTR-IT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9753689}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1bcg]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Hottentotta_judaicus Hottentotta judaicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1BCG OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009753689<~~/~~ref~~><~~ref group="xtra">PMID:019113835</ref><references group="xtra"~~/>	+
	[[Category: Hottentotta judaicus]]		[[Category: Hottentotta judaicus]]
	[[Category: Amit, E.]]		[[Category: Amit, E.]]

OCA: Protected "1bcg" [edit=sysop:move=sysop]

2012-10-21T14:23:24Z

Protected "1bcg" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:23, 21 October 2012