1bge - Revision history

OCA at 15:32, 13 March 2024

2024-03-13T15:32:45Z

←Older revision		Revision as of 15:32, 13 March 2024
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	<StructureSection load='1bge' size='340' side='right'caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1bge' size='340' side='right'caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Canlf Canlf~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [https://pdbe.org/1bge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [https://www.ebi.ac.uk/pdbsum/1bge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bge ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [https://pdbe.org/1bge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [https://www.ebi.ac.uk/pdbsum/1bge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CSF3_CANFA CSF3_CANFA]~~] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.	+	[https://www.uniprot.org/uniprot/CSF3_CANLF CSF3_CANLF] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bge ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bge ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structures of recombinant canine and bovine granulocyte colony stimulating factor (G-CSF) have been determined by X-ray crystallography, using molecular replacement with recombinant human G-CSF as a model. G-CSF is a member of the cytokine family of glycoproteins that stimulate the differentiation and proliferation of blood cells. Human, bovine and canine G-CSF all have a molecular mass of about 19 kDa and share an amino acid sequence identity of about 80%. Two crystal forms of canine G-CSF have been solved. Form I recombinant canine G-CSF (rcG-CSFI; space group C2) contains one molecule in the asymmetric unit while form II canine G-CSF (rcG-CSFII; space group P2(1)) has two molecules in the asymmetric unit and bovine G-CSF (rbG-CSF; space group P2(1)2(1)2(1)) contains one molecule in the asymmetric unit. rcG-CSFI has been refined to an R factor of 20.7% with data to 2.3 A resolution and rcG-CSFII has been refined to an R factor of 19.3% with data to 2.2 A resolution. rbG-CSF has been refined to an R factor of 21.3% with data to 1.7 A resolution. The structure of human, canine and bovine G-CSF is an antiparallel 4-alpha-helical bundle with up-up-down-down connectivity. With the exception of one highly exposed loop (residues 66 to 74), the human, canine and bovine structures are very similar to each other. Using our series of G-CSF crystal structures we developed a function that describes the probability that a particular residue position (i) contributes to a G-CSF receptor binding site based on two principles, (1) high sequence conservation in the primary sequence of human, bovine, canine and murine G-CSF and (2) conservation of high solvent accessibility in the human, bovine and canine crystal structures. On the basis of this probability function as well as a comparison of G-CSF to the crystal structure of human growth hormone (hGH) complexed with the extracellular domain of the human growth hormone receptor (hGHbp), residues that contribute to potential G-CSF receptor binding sites are identified.
-
-	Crystal structure of canine and bovine granulocyte-colony stimulating factor (G-CSF).,Lovejoy B, Cascio D, Eisenberg D J Mol Biol. 1993 Dec 5;234(3):640-53. PMID:7504736<ref>PMID:7504736</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1bge" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Colony-stimulating factor 3D structures\|Colony-stimulating factor 3D structures]]		*[[Colony-stimulating factor 3D structures\|Colony-stimulating factor 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canlf~~]]	+	[[Category: Canis lupus familiaris]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cascio, D]]	+	[[Category: Cascio D]]
-	[[Category: Eisenberg, D]]	+	[[Category: Eisenberg D]]
-	[[Category: Lovejoy, B]]	+	[[Category: Lovejoy B]]
-	~~[[Category: Cytokine~~]]	+

OCA at 10:40, 19 May 2021

2021-05-19T10:40:33Z

←Older revision		Revision as of 10:40, 19 May 2021
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	<StructureSection load='1bge' size='340' side='right'caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1bge' size='340' side='right'caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [~~http~~://pdbe.org/1bge PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bge PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bge ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [https://pdbe.org/1bge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [https://www.ebi.ac.uk/pdbsum/1bge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CSF3_CANFA CSF3_CANFA]] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.	+	[[https://www.uniprot.org/uniprot/CSF3_CANFA CSF3_CANFA]] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:03, 14 August 2019

2019-08-14T18:03:00Z

←Older revision		Revision as of 18:03, 14 August 2019
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	==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==		==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==
-	<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1bge' size='340' side='right'caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/1bge_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/1bge_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1bge" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1bge" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Colony-stimulating factor 3D structures\|Colony-stimulating factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Canlf]]		[[Category: Canlf]]
		+	[[Category: Large Structures]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Eisenberg, D]]		[[Category: Eisenberg, D]]
	[[Category: Lovejoy, B]]		[[Category: Lovejoy, B]]
	[[Category: Cytokine]]		[[Category: Cytokine]]

OCA at 10:43, 8 November 2017

2017-11-08T10:43:06Z

←Older revision		Revision as of 10:43, 8 November 2017
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		+
	==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==		==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==
	<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Canfa Canfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canlf Canlf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://pdbe.org/1bge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://pdbe.org/1bge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bge ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canfa~~]]	+	[[Category: Canlf]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Eisenberg, D]]		[[Category: Eisenberg, D]]
	[[Category: Lovejoy, B]]		[[Category: Lovejoy, B]]
	[[Category: Cytokine]]		[[Category: Cytokine]]

OCA at 02:54, 7 February 2016

2016-02-07T02:54:06Z

←Older revision		Revision as of 02:54, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bge ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:58, 9 September 2015

2015-09-09T20:58:37Z

←Older revision		Revision as of 20:58, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Canis_lupus_familiaris Canis lupus familiaris~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canfa Canfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://pdbe.org/1bge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bge" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canis lupus familiaris~~]]	+	[[Category: Canfa]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Eisenberg, D]]		[[Category: Eisenberg, D]]
	[[Category: Lovejoy, B]]		[[Category: Lovejoy, B]]
	[[Category: Cytokine]]		[[Category: Cytokine]]

OCA at 07:16, 24 December 2014

2014-12-24T07:16:16Z

←Older revision		Revision as of 07:16, 24 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSF3_CANFA CSF3_CANFA]] Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:00, 22 December 2014

2014-12-22T09:00:17Z

←Older revision		Revision as of 09:00, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Canis lupus familiaris]]		[[Category: Canis lupus familiaris]]
-	[[Category: Cascio, D.]]	+	[[Category: Cascio, D]]
-	[[Category: Eisenberg, D.]]	+	[[Category: Eisenberg, D]]
-	[[Category: Lovejoy, B.]]	+	[[Category: Lovejoy, B]]
	[[Category: Cytokine]]		[[Category: Cytokine]]

OCA at 03:08, 7 August 2014

2014-08-07T03:08:20Z

←Older revision		Revision as of 03:08, 7 August 2014
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-	[[~~Image~~:1bge.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)==
		+	<StructureSection load='1bge' size='340' side='right' caption='[[1bge]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bge]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BGE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bge OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bge RCSB], [http://www.ebi.ac.uk/pdbsum/1bge PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/1bge_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of recombinant canine and bovine granulocyte colony stimulating factor (G-CSF) have been determined by X-ray crystallography, using molecular replacement with recombinant human G-CSF as a model. G-CSF is a member of the cytokine family of glycoproteins that stimulate the differentiation and proliferation of blood cells. Human, bovine and canine G-CSF all have a molecular mass of about 19 kDa and share an amino acid sequence identity of about 80%. Two crystal forms of canine G-CSF have been solved. Form I recombinant canine G-CSF (rcG-CSFI; space group C2) contains one molecule in the asymmetric unit while form II canine G-CSF (rcG-CSFII; space group P2(1)) has two molecules in the asymmetric unit and bovine G-CSF (rbG-CSF; space group P2(1)2(1)2(1)) contains one molecule in the asymmetric unit. rcG-CSFI has been refined to an R factor of 20.7% with data to 2.3 A resolution and rcG-CSFII has been refined to an R factor of 19.3% with data to 2.2 A resolution. rbG-CSF has been refined to an R factor of 21.3% with data to 1.7 A resolution. The structure of human, canine and bovine G-CSF is an antiparallel 4-alpha-helical bundle with up-up-down-down connectivity. With the exception of one highly exposed loop (residues 66 to 74), the human, canine and bovine structures are very similar to each other. Using our series of G-CSF crystal structures we developed a function that describes the probability that a particular residue position (i) contributes to a G-CSF receptor binding site based on two principles, (1) high sequence conservation in the primary sequence of human, bovine, canine and murine G-CSF and (2) conservation of high solvent accessibility in the human, bovine and canine crystal structures. On the basis of this probability function as well as a comparison of G-CSF to the crystal structure of human growth hormone (hGH) complexed with the extracellular domain of the human growth hormone receptor (hGHbp), residues that contribute to potential G-CSF receptor binding sites are identified.

-	~~{{STRUCTURE_1bge\| PDB=1bge \| SCENE= }}~~	+	Crystal structure of canine and bovine granulocyte-colony stimulating factor (G-CSF).,Lovejoy B, Cascio D, Eisenberg D J Mol Biol. 1993 Dec 5;234(3):640-53. PMID:7504736<ref>PMID:7504736</ref>

-	~~===CRYSTAL STRUCTURE OF CANINE AND BOVINE GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7504736}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1bge]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Canis_lupus_familiaris Canis lupus familiaris]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1BGE OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007504736<~~/~~ref~~><~~ref group="xtra">PMID:012215419</ref><references group="xtra"~~/>	+
	[[Category: Canis lupus familiaris]]		[[Category: Canis lupus familiaris]]
	[[Category: Cascio, D.]]		[[Category: Cascio, D.]]

OCA: Protected "1bge" [edit=sysop:move=sysop]

2012-10-21T20:29:53Z

Protected "1bge" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:29, 21 October 2012