1bwl - Revision history

OCA at 05:44, 9 August 2023

2023-08-09T05:44:31Z

←Older revision		Revision as of 05:44, 9 August 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [https://pdbe.org/1bwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [https://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [https://pdbe.org/1bwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [https://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>
	</table>		</table>

OCA at 04:28, 30 September 2022

2022-09-30T04:28:30Z

←Older revision		Revision as of 04:28, 30 September 2022
Line 3:		Line 3:
	<StructureSection load='1bwl' size='340' side='right'caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1bwl' size='340' side='right'caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_11006_[[monilia_stellatoidea]] Atcc 11006 [[monilia stellatoidea]]~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Monilia stellatoidea]]])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [https://pdbe.org/1bwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [https://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [https://pdbe.org/1bwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [https://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS]] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.	+	[https://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candida albicans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: NADPH dehydrogenase]]~~	+	[[Category: Brown BJ]]
-	[[Category: Brown~~, B J~~]]	+	[[Category: Deng Z]]
-	[[Category: Deng, Z]]	+	[[Category: Karplus PA]]
-	[[Category: Karplus~~, P A~~]]	+	[[Category: Massey V]]
-	[[Category: Massey, V]]	+
-	~~[[Category: Flavoenzyme]]~~	+
-	~~[[Category: Nadph oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 14:49, 3 November 2021

2021-11-03T14:49:05Z

←Older revision		Revision as of 14:49, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='1bwl' size='340' side='right'caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1bwl' size='340' side='right'caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_11006_[[~~candida_stellatoidea~~]] Atcc 11006 [[~~candida~~ stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11006_[[monilia_stellatoidea]] Atcc 11006 [[monilia stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[~~Candida~~ stellatoidea]]])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Monilia stellatoidea]]])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [~~http~~://pdbe.org/1bwl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [https://pdbe.org/1bwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [https://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS]] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.	+	[[https://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS]] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:16, 18 November 2020

2020-11-18T10:16:03Z

←Older revision		Revision as of 10:16, 18 November 2020
Line 1:		Line 1:

	==OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H==		==OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H==
-	<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='1bwl' size='340' side='right'caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1BWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://pdbe.org/1bwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://pdbe.org/1bwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/1bwl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/1bwl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1bwl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1bwl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[NADPH dehydrogenase\|NADPH dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: NADPH dehydrogenase]]		[[Category: NADPH dehydrogenase]]
	[[Category: Brown, B J]]		[[Category: Brown, B J]]

OCA at 08:05, 29 November 2017

2017-11-29T08:05:45Z

←Older revision		Revision as of 08:05, 29 November 2017
Line 1:		Line 1:
		+
	==OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H==		==OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H==
	<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://pdbe.org/1bwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://pdbe.org/1bwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1bwl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1bwl" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[NADPH dehydrogenase\|NADPH dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:27, 8 February 2016

2016-02-08T05:27:35Z

←Older revision		Revision as of 05:27, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bwl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:20, 10 September 2015

2015-09-10T08:20:37Z

←Older revision		Revision as of 08:20, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_albicans Candida albicans~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BWL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida ~~albicans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://pdbe.org/1bwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1bwl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Candida albicans]]
	[[Category: NADPH dehydrogenase]]		[[Category: NADPH dehydrogenase]]
	[[Category: Brown, B J]]		[[Category: Brown, B J]]

OCA at 20:57, 24 December 2014

2014-12-24T20:57:34Z

←Older revision		Revision as of 20:57, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OYE1_SACPS OYE1_SACPS]] Oxidizes beta-NADH, beta-NADPH, and alpha-NADPH.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:32, 22 December 2014

2014-12-22T10:32:40Z

←Older revision		Revision as of 10:32, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BWL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: NADPH dehydrogenase]]		[[Category: NADPH dehydrogenase]]
-	[[Category: Brown, B J.]]	+	[[Category: Brown, B J]]
-	[[Category: Deng, Z.]]	+	[[Category: Deng, Z]]
-	[[Category: Karplus, P A.]]	+	[[Category: Karplus, P A]]
-	[[Category: Massey, V.]]	+	[[Category: Massey, V]]
	[[Category: Flavoenzyme]]		[[Category: Flavoenzyme]]
	[[Category: Nadph oxidoreductase]]		[[Category: Nadph oxidoreductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 09:36, 13 August 2014

2014-08-13T09:36:18Z

←Older revision		Revision as of 09:36, 13 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1bwl.~~png~~\|~~left~~\|200px]]	+	==OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H==
		+	<StructureSection load='1bwl' size='340' side='right' caption='[[1bwl]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1bwl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BWL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">OYE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 Candida albicans])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bwl RCSB], [http://www.ebi.ac.uk/pdbsum/1bwl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/1bwl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Old Yellow Enzyme (OYE) binds phenolic ligands forming long wavelength (500-800 nm) charge-transfer complexes. The enzyme is reduced by NADPH, and oxygen, quinones, and alpha,beta-unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. Solution of the crystal structure of OYE1 from brewer's bottom yeast (Fox, K. M., and Karplus, P. A. (1994) Structure 2, 1089-1105) made it possible to identify histidine 191 and asparagine 194 as amino acid residues that hydrogen-bond with the phenolic ligands, stabilizing the anionic form involved in charge-transfer interaction with the FMN prosthetic group. His-191 and Asn-194 are also predicted to interact with the nicotinamide ring of NADPH in the active site. Mutations of His-191 to Asn, Asn-194 to His, and a double mutation, H191N/N194H, were made of OYE1. It was not possible to isolate the N191H mutant enzyme, but the other two mutant forms had the expected effect on phenolic ligand binding, i.e. decreased binding affinity and decreased charge-transfer absorbance. Reduction of the H191N mutant enzyme by NADPH was similar to that of OYE1, but the reduction rate constant for NADH was greatly decreased. The double mutant enzyme had an increased rate constant for reduction by NADPH, but the reduction rate constant with NADH was lower by a factor of 15. The reactivity of OYE1 and the mutant enzymes with oxygen was similar, but the reactivity of 2-cyclohexenone was greatly decreased by the mutations. The crystal structures of the two mutant forms showed only minor changes from that of the wild type enzyme.

-	~~{{STRUCTURE_1bwl\| PDB=1bwl \| SCENE= }}~~	+	On the active site of Old Yellow Enzyme. Role of histidine 191 and asparagine 194.,Brown BJ, Deng Z, Karplus PA, Massey V J Biol Chem. 1998 Dec 4;273(49):32753-62. PMID:9830019<ref>PMID:9830019</ref>

-	~~===OLD YELLOW ENZYME (OYE1) DOUBLE MUTANT H191N:N194H===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_9830019}}~~	+	==See Also==
-		+	*[[NADPH dehydrogenase\|NADPH dehydrogenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1bwl~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWL OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:009830019<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: NADPH dehydrogenase]]		[[Category: NADPH dehydrogenase]]