1c72 - Revision history

OCA at 05:48, 9 August 2023

2023-08-09T05:48:57Z

←Older revision		Revision as of 05:48, 9 August 2023
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	<StructureSection load='1c72' size='340' side='right'caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1c72' size='340' side='right'caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EPY:1-HYDROXY~~-2~~-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~2~~.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [https://pdbe.org/1c72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [https://www.ebi.ac.uk/pdbsum/1c72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c72 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [https://pdbe.org/1c72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [https://www.ebi.ac.uk/pdbsum/1c72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c72 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GSTM2_CHICK GSTM2_CHICK]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.	+	[https://www.uniprot.org/uniprot/GSTM2_CHICK GSTM2_CHICK] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick]]~~	+	[[Category: Gallus gallus]]
-	~~[[Category: Glutathione transferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chern~~, M K~~]]	+	[[Category: Chern MK]]
-	[[Category: Chou~~, C C~~]]	+	[[Category: Chou CC]]
-	[[Category: Hsiao~~, C D~~]]	+	[[Category: Hsiao CD]]
-	[[Category: Hsieh~~, C H~~]]	+	[[Category: Hsieh CH]]
-	[[Category: Kuan~~, I C~~]]	+	[[Category: Kuan IC]]
-	[[Category: Liu~~, L F~~]]	+	[[Category: Liu LF]]
-	[[Category: Tam~~, M F~~]]	+	[[Category: Tam MF]]
-	[[Category: Wu~~, T C~~]]	+	[[Category: Wu TC]]
-	[[Category: Yeh~~, Y H]]~~	+	[[Category: Yeh YH]]
-	~~[[Category: Epoxidase activity]]~~	+
-	~~[[Category: Glutathione s-transferase]]~~	+
-	~~[[Category: Steady-state kinetic]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:33, 14 July 2021

2021-07-14T10:33:40Z

←Older revision		Revision as of 10:33, 14 July 2021
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	<StructureSection load='1c72' size='340' side='right'caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1c72' size='340' side='right'caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1C72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [~~http~~://pdbe.org/1c72 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1c72 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1c72 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [https://pdbe.org/1c72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [https://www.ebi.ac.uk/pdbsum/1c72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c72 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GSTM2_CHICK GSTM2_CHICK]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.	+	[[https://www.uniprot.org/uniprot/GSTM2_CHICK GSTM2_CHICK]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:02, 10 October 2019

2019-10-10T07:02:11Z

←Older revision		Revision as of 07:02, 10 October 2019
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	==TYR115, GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE S-TRANSFERASE CGSTM1-1==		==TYR115, GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE S-TRANSFERASE CGSTM1-1==
-	<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1c72' size='340' side='right'caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/1c72_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/1c72_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1c72" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1c72" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Chick]]		[[Category: Chick]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
		+	[[Category: Large Structures]]
	[[Category: Chern, M K]]		[[Category: Chern, M K]]
	[[Category: Chou, C C]]		[[Category: Chou, C C]]

OCA at 08:21, 29 November 2017

2017-11-29T08:21:08Z

←Older revision		Revision as of 08:21, 29 November 2017
Line 1:		Line 1:
		+
	==TYR115, GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE S-TRANSFERASE CGSTM1-1==		==TYR115, GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE S-TRANSFERASE CGSTM1-1==
	<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://pdbe.org/1c72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://pdbe.org/1c72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1c72 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1c72" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1c72" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:37, 9 February 2016

2016-02-09T20:37:52Z

←Older revision		Revision as of 20:37, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c72 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:50, 11 September 2015

2015-09-11T01:50:06Z

←Older revision		Revision as of 01:50, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://pdbe.org/1c72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1c72" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Chern, M K]]		[[Category: Chern, M K]]

OCA at 09:23, 25 December 2014

2014-12-25T09:23:31Z

←Older revision		Revision as of 09:23, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GSTM2_CHICK GSTM2_CHICK]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:56, 22 December 2014

2014-12-22T08:56:24Z

←Older revision		Revision as of 08:56, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: Chern, M K.]]	+	[[Category: Chern, M K]]
-	[[Category: Chou, C C.]]	+	[[Category: Chou, C C]]
-	[[Category: Hsiao, C D.]]	+	[[Category: Hsiao, C D]]
-	[[Category: Hsieh, C H.]]	+	[[Category: Hsieh, C H]]
-	[[Category: Kuan, I C.]]	+	[[Category: Kuan, I C]]
-	[[Category: Liu, L F.]]	+	[[Category: Liu, L F]]
-	[[Category: Tam, M F.]]	+	[[Category: Tam, M F]]
-	[[Category: Wu, T C.]]	+	[[Category: Wu, T C]]
-	[[Category: Yeh, Y H.]]	+	[[Category: Yeh, Y H]]
	[[Category: Epoxidase activity]]		[[Category: Epoxidase activity]]
	[[Category: Glutathione s-transferase]]		[[Category: Glutathione s-transferase]]
	[[Category: Steady-state kinetic]]		[[Category: Steady-state kinetic]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 17:15, 20 August 2014

2014-08-20T17:15:04Z

←Older revision		Revision as of 17:15, 20 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1c72.~~png\|left~~\|200px]]	+	==TYR115, GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE S-TRANSFERASE CGSTM1-1==
		+	<StructureSection load='1c72' size='340' side='right' caption='[[1c72]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1c72]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C72 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPY:1-HYDROXY-2-S-GLUTATHIONYL-3-PARA-NITROPHENOXY-PROPANE'>EPY</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1c72 RCSB], [http://www.ebi.ac.uk/pdbsum/1c72 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/1c72_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We investigated the epoxidase activity of a class mu glutathione S-transferase (cGSTM1-1), using 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) as substrate. Trp209 on the C-terminal tail, Arg107 on the alpha4 helix, Asp161 and Gln165 on the alpha6 helix of cGSTM1-1 were selected for mutagenesis and kinetic studies. A hydrophobic side-chain at residue 209 is needed for the epoxidase activity of cGSTM1-1. Replacing Trp209 with histidine, isoleucine or proline resulted in a fivefold to 28-fold decrease in the k(cat)(app) of the enzyme, while a modest 25 % decrease in the k(cat)(app) was observed for the W209F mutant. The rGSTM1-1 enzyme has serine at the correponding position. The k(cat)(app) of the S209W mutant is 2. 5-fold higher than that of the wild-type rGSTM1-1. A charged residue is needed at position 107 of cGSTM1-1. The K(m)(app)(GSH) of the R107L mutant is 38-fold lower than that of the wild-type enzyme. On the contrary, the R107E mutant has a K(m)(app)(GSH) and a k(cat)(app) that are 11-fold and 35 % lower than those of the wild-type cGSTM1-1. The substitutions of Gln165 with Glu or Leu have minimal effect on the affinity of the mutants towards GSH or EPNP. However, a discernible reduction in k(cat)(app) was observed. Asp161 is involved in maintaining the structural integrity of the enzyme. The K(m)(app)(GSH) of the D161L mutant is 616-fold higher than that of the wild-type enzyme. In the hydrogen/deuterium exchange experiments, this mutant has the highest level of deuteration among all the proteins tested.We also elucidated the structure of cGSTM1-1 co-crystallized with the glutathionyl-conjugated 1, 2-epoxy-3-(p-nitrophenoxy)propane (EPNP) at 2.8 A resolution. The product found in the active site was 1-hydroxy-2-(S-glutathionyl)-3-(p-nitrophenoxy)propane, instead of the conventional 2-hydroxy isomer. The EPNP moiety orients towards Arg107 and Gln165 in dimer AB, and protrudes into a hydrophobic region formed by the loop connecting beta1 and alpha1 and part of the C-terminal tail in dimer CD. The phenoxyl ring forms strong ring stacking with the Trp209 side-chain in dimer CD. We hypothesize that these two conformations represent the EPNP moiety close to the initial and final stages of the reaction mechanism, respectively.

-	~~{{STRUCTURE_1c72\| PDB=1c72 \| SCENE= }}~~	+	Tyr115, gln165 and trp209 contribute to the 1, 2-epoxy-3-(p-nitrophenoxy)propane-conjugating activity of glutathione S-transferase cGSTM1-1.,Chern MK, Wu TC, Hsieh CH, Chou CC, Liu LF, Kuan IC, Yeh YH, Hsiao CD, Tam MF J Mol Biol. 2000 Jul 28;300(5):1257-69. PMID:10903867<ref>PMID:10903867</ref>

-	~~===TYR115~~, ~~GLN165 AND TRP209 CONTRIBUTE TO THE 1,2-EPOXY-3-(P-NITROPHENOXY)PROPANE CONJUGATING ACTIVITIES OF GLUTATHIONE~~ S~~-TRANSFERASE CGSTM1-1===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10903867}}~~	+	==See Also==
-		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1c72~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C72 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010903867<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]

OCA: Protected "1c72" [edit=sysop:move=sysop]

2012-10-21T20:25:55Z

Protected "1c72" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:25, 21 October 2012