1cc0 - Revision history

OCA at 23:25, 27 December 2023

2023-12-27T23:25:24Z

←Older revision		Revision as of 23:25, 27 December 2023
Line 3:		Line 3:
	<StructureSection load='1cc0' size='340' side='right'caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='1cc0' size='340' side='right'caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [https://pdbe.org/1cc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cc0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [https://pdbe.org/1cc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref> [[https://www.uniprot.org/uniprot/GDIR1_HUMAN GDIR1_HUMAN]] Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity).	+	[https://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dauter, Z]]	+	[[Category: Dauter Z]]
-	[[Category: Derewenda, U]]	+	[[Category: Derewenda U]]
-	[[Category: Derewenda~~, Z S~~]]	+	[[Category: Derewenda ZS]]
-	[[Category: Garrard, S]]	+	[[Category: Garrard S]]
-	[[Category: Longenecker~~, K L~~]]	+	[[Category: Longenecker KL]]
-	[[Category: Nakamoto~~, R K~~]]	+	[[Category: Nakamoto RK]]
-	[[Category: Read, P]]	+	[[Category: Read P]]
-	[[Category: Somlyo~~, A P~~]]	+	[[Category: Somlyo AP]]
-	[[Category: Somlyo~~, A V~~]]	+	[[Category: Somlyo AV]]
-	[[Category: Walker, L]]	+	[[Category: Walker L]]
-	~~[[Category: G-protein]]~~	+
-	~~[[Category: Rho gtpase]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 06:28, 11 August 2021

2021-08-11T06:28:28Z

←Older revision		Revision as of 06:28, 11 August 2021
Line 3:		Line 3:
	<StructureSection load='1cc0' size='340' side='right'caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='1cc0' size='340' side='right'caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [~~http~~://pdbe.org/1cc0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cc0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cc0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [https://pdbe.org/1cc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref> [[~~http~~://www.uniprot.org/uniprot/GDIR1_HUMAN GDIR1_HUMAN]] Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity).	+	[[https://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref> [[https://www.uniprot.org/uniprot/GDIR1_HUMAN GDIR1_HUMAN]] Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]		*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	*[[Guanine nucleotide dissociation inhibitor\|Guanine nucleotide dissociation inhibitor]]		*[[Guanine nucleotide dissociation inhibitor\|Guanine nucleotide dissociation inhibitor]]
-	*[[Rho GTPase\|Rho GTPase]]	+	*[[Rho GTPase 3D structures\|Rho GTPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:34, 10 October 2019

2019-10-10T06:34:24Z

←Older revision		Revision as of 06:34, 10 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX==		==CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX==
-	<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>	+	<StructureSection load='1cc0' size='340' side='right'caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/1cc0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/1cc0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1cc0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cc0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
		+	*[[Guanine nucleotide dissociation inhibitor\|Guanine nucleotide dissociation inhibitor]]
		+	*[[Rho GTPase\|Rho GTPase]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]
	[[Category: Derewenda, U]]		[[Category: Derewenda, U]]

OCA at 08:02, 29 November 2017

2017-11-29T08:02:35Z

←Older revision		Revision as of 08:02, 29 November 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX==		==CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX==
	<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://pdbe.org/1cc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://pdbe.org/1cc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1cc0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cc0" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[GTP-binding protein\|GTP-binding protein]]
-	*[[Guanine nucleotide dissociation inhibitor\|Guanine nucleotide dissociation inhibitor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:33, 9 February 2016

2016-02-09T10:33:15Z

←Older revision		Revision as of 10:33, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cc0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:24, 10 September 2015

2015-09-10T21:24:56Z

←Older revision		Revision as of 21:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>		<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://pdbe.org/1cc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cc0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Dauter, Z]]		[[Category: Dauter, Z]]
	[[Category: Derewenda, U]]		[[Category: Derewenda, U]]

OCA at 06:17, 25 December 2014

2014-12-25T06:17:40Z

←Older revision		Revision as of 06:17, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN]] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref> [[http://www.uniprot.org/uniprot/GDIR1_HUMAN GDIR1_HUMAN]] Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1 (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:21, 22 December 2014

2014-12-22T11:21:51Z

←Older revision		Revision as of 11:21, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dauter, Z.]]	+	[[Category: Dauter, Z]]
-	[[Category: Derewenda, U.]]	+	[[Category: Derewenda, U]]
-	[[Category: Derewenda, Z S.]]	+	[[Category: Derewenda, Z S]]
-	[[Category: Garrard, S.]]	+	[[Category: Garrard, S]]
-	[[Category: Longenecker, K L.]]	+	[[Category: Longenecker, K L]]
-	[[Category: Nakamoto, R K.]]	+	[[Category: Nakamoto, R K]]
-	[[Category: Read, P.]]	+	[[Category: Read, P]]
-	[[Category: Somlyo, A P.]]	+	[[Category: Somlyo, A P]]
-	[[Category: Somlyo, A V.]]	+	[[Category: Somlyo, A V]]
-	[[Category: Walker, L.]]	+	[[Category: Walker, L]]
	[[Category: G-protein]]		[[Category: G-protein]]
	[[Category: Rho gtpase]]		[[Category: Rho gtpase]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 16:53, 20 August 2014

2014-08-20T16:53:23Z

←Older revision		Revision as of 16:53, 20 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cc0.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX==
		+	<StructureSection load='1cc0' size='340' side='right' caption='[[1cc0]], [[Resolution\|resolution]] 5.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CC0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cc0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/1cc0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Like all Rho (Ras homology) GTPases, RhoA functions as a molecular switch in cell signaling, alternating between GTP- and GDP-bound states, with its biologically inactive GDP-bound form maintained as a cytosolic complex with RhoGDI (guanine nucleotide-exchange inhibitor). The crystal structures of RhoA-GDP and of the C-terminal immunoglobulin-like domain of RhoGDI (residues 67-203) are known, but the mechanism by which the two proteins interact is not known. The functional human RhoA-RhoGDI complex has been expressed in yeast and crystallized (P6(5)22, unit-cell parameters a = b = 139, c = 253 A, two complexes in the asymmetric unit). Although diffraction from these crystals extends to 3.5 A and is highly anisotropic, the experimentally phased (MAD plus MIR) electron-density map was adequate to reveal the mutual disposition of the two molecules. The result was validated by molecular-replacement calculations when data were corrected for anisotropy. Furthermore, the N-terminus of RhoGDI (the region involved in inhibition of nucleotide exchange) can be identified in the electron-density map: it is bound to the switch I and switch II regions of RhoA, occluding an epitope which binds Dbl-like nucleotide-exchange factors. The entrance of the hydrophobic pocket of RhoGDI is 25 A from the last residue in the RhoA model, with its C-terminus oriented to accommodate the geranylgeranyl group without conformational change in RhoA.

-	~~{{STRUCTURE_1cc0\| PDB=1cc0 \| SCENE= }}~~	+	How RhoGDI binds Rho.,Longenecker K, Read P, Derewenda U, Dauter Z, Liu X, Garrard S, Walker L, Somlyo AV, Nakamoto RK, Somlyo AP, Derewenda ZS Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1503-15. PMID:10489445<ref>PMID:10489445</ref>

-	~~===CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10489445}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1cc0]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA].~~	+

	==See Also==		==See Also==
	*[[GTP-binding protein\|GTP-binding protein]]		*[[GTP-binding protein\|GTP-binding protein]]
-		+	*[[Guanine nucleotide dissociation inhibitor\|Guanine nucleotide dissociation inhibitor]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:010489445<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dauter, Z.]]		[[Category: Dauter, Z.]]

OCA at 13:35, 20 October 2012

2012-10-20T13:35:18Z

←Older revision		Revision as of 13:35, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1cc0]] is a 4 chain structure ~~of [[GTP-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA].	+	[[1cc0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CC0 OCA].

	==See Also==		==See Also==