1cld - Revision history

OCA at 08:21, 22 May 2024

2024-05-22T08:21:42Z

←Older revision		Revision as of 08:21, 22 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cld ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cld ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
		+
		+	Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.,Gardner KH, Anderson SF, Coleman JE Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715<ref>PMID:7552715</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1cld" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[CD2\|CD2]]		*[[CD2\|CD2]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:40, 13 March 2024

2024-03-13T15:40:37Z

←Older revision		Revision as of 15:40, 13 March 2024
Line 1:		Line 1:

	==DNA-binding protein==		==DNA-binding protein==
-	<StructureSection load='1cld' size='340' side='right'caption='[[1cld~~]], [[NMR_Ensembles_of_Models \| 29 NMR models~~]]' scene=''>	+	<StructureSection load='1cld' size='340' side='right'caption='[[1cld]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_56498 Atcc 56498~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [https://pdbe.org/1cld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [https://www.ebi.ac.uk/pdbsum/1cld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cld ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [https://pdbe.org/1cld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [https://www.ebi.ac.uk/pdbsum/1cld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cld ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LAC9_KLULA LAC9_KLULA]] Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.	+	[https://www.uniprot.org/uniprot/LAC9_KLULA LAC9_KLULA] Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cld ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cld ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
-
-	Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.,Gardner KH, Anderson SF, Coleman JE Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715<ref>PMID:7552715</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cld" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[CD2\|CD2]]		*[[CD2\|CD2]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 56498~~]]	+	[[Category: Kluyveromyces lactis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coleman~~, J E~~]]	+	[[Category: Coleman JE]]
-	[[Category: Gardner~~, K H]]~~	+	[[Category: Gardner KH]]
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Zinc-binding domain~~]]	+

OCA at 10:37, 14 July 2021

2021-07-14T10:37:41Z

←Older revision		Revision as of 10:37, 14 July 2021
Line 3:		Line 3:
	<StructureSection load='1cld' size='340' side='right'caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1cld' size='340' side='right'caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [~~http~~://pdbe.org/1cld PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cld PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cld ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [https://pdbe.org/1cld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [https://www.ebi.ac.uk/pdbsum/1cld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cld ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LAC9_KLULA LAC9_KLULA]] Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.	+	[[https://www.uniprot.org/uniprot/LAC9_KLULA LAC9_KLULA]] Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:01, 10 October 2019

2019-10-10T07:01:10Z

←Older revision		Revision as of 07:01, 10 October 2019
Line 1:		Line 1:

	==DNA-binding protein==		==DNA-binding protein==
-	<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>	+	<StructureSection load='1cld' size='340' side='right'caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/1cld_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/1cld_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 56498]]		[[Category: Atcc 56498]]
		+	[[Category: Large Structures]]
	[[Category: Coleman, J E]]		[[Category: Coleman, J E]]
	[[Category: Gardner, K H]]		[[Category: Gardner, K H]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Zinc-binding domain]]		[[Category: Zinc-binding domain]]

OCA at 08:20, 29 November 2017

2017-11-29T08:20:23Z

←Older revision		Revision as of 08:20, 29 November 2017
Line 1:		Line 1:
		+
	==DNA-binding protein==		==DNA-binding protein==
	<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_sphaerica Candida sphaerica~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://pdbe.org/1cld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://pdbe.org/1cld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cld ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Candida sphaerica~~]]	+	[[Category: Atcc 56498]]
	[[Category: Coleman, J E]]		[[Category: Coleman, J E]]
	[[Category: Gardner, K H]]		[[Category: Gardner, K H]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Zinc-binding domain]]		[[Category: Zinc-binding domain]]

OCA at 15:09, 9 February 2016

2016-02-09T15:09:31Z

←Older revision		Revision as of 15:09, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cld ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:26, 10 September 2015

2015-09-10T23:26:54Z

←Older revision		Revision as of 23:26, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Kluyveromyces_lactis Kluyveromyces lactis~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://pdbe.org/1cld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cld" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Kluyveromyces lactis~~]]	+	[[Category: Candida sphaerica]]
	[[Category: Coleman, J E]]		[[Category: Coleman, J E]]
	[[Category: Gardner, K H]]		[[Category: Gardner, K H]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Zinc-binding domain]]		[[Category: Zinc-binding domain]]

OCA at 07:36, 25 December 2014

2014-12-25T07:36:53Z

←Older revision		Revision as of 07:36, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LAC9_KLULA LAC9_KLULA]] Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:33, 22 December 2014

2014-12-22T19:33:30Z

←Older revision		Revision as of 19:33, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
-	[[Category: Coleman, J E.]]	+	[[Category: Coleman, J E]]
-	[[Category: Gardner, K H.]]	+	[[Category: Gardner, K H]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Zinc-binding domain]]		[[Category: Zinc-binding domain]]

OCA at 09:27, 27 August 2014

2014-08-27T09:27:38Z

←Older revision		Revision as of 09:27, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cld.~~png\|left~~\|200px]]	+	==DNA-binding protein==
		+	<StructureSection load='1cld' size='340' side='right' caption='[[1cld]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/1cld_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

-	~~{{STRUCTURE_1cld\| PDB=1cld \| SCENE= }}~~	+	Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.,Gardner KH, Anderson SF, Coleman JE Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715<ref>PMID:7552715</ref>

-	~~===DNA-binding protein===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_7552715}}~~	+	==See Also==
-		+	*[[CD2\|CD2]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1cld~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLD OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:007552715<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Kluyveromyces lactis]]		[[Category: Kluyveromyces lactis]]
	[[Category: Coleman, J E.]]		[[Category: Coleman, J E.]]