1clw - Revision history

OCA at 05:53, 9 August 2023

2023-08-09T05:53:50Z

←Older revision		Revision as of 05:53, 9 August 2023
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	<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpp22 Bpp22~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~9 (~~[~~https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10754 BPP22])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [https://pdbe.org/1clw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [https://www.ebi.ac.uk/pdbsum/1clw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [https://pdbe.org/1clw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [https://www.ebi.ac.uk/pdbsum/1clw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TSPE_BPP22 TSPE_BPP22~~]~~] Non-covalently bound to the neck~~ of the ~~phage capsid and mediating~~ attachment ~~of the viral particle~~ to host cell~~-surface polysaccharide. It displays~~ endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.<ref>PMID:12837775</ref> <ref>PMID:20817910</ref>	+	[https://www.uniprot.org/uniprot/FIBER_BPP22 FIBER_BPP22] Structural component of the short non-contractile tail. The tail comprises six fibers that mediate primary attachment to the host cell lipopolysaccharides (LPS) and display endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide. Digestion of the LPS brings the capsid near the cell outer membrane.<ref>PMID:12837775</ref> <ref>PMID:20817910</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bpp22]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baxa, U]]	+	[[Category: Salmonella virus P22]]
-	[[Category: Huber, R]]	+	[[Category: Baxa U]]
-	[[Category: Seckler, R]]	+	[[Category: Huber R]]
-	[[Category: Steinbacher, S]]	+	[[Category: Seckler R]]
-	[[Category: Weintraub, A]]	+	[[Category: Steinbacher S]]
-	~~[[Category: Late protein]]~~	+	[[Category: Weintraub A]]
-	~~[[Category: Viral protein]]~~	+
-	~~[[Category: Virus/viral protein~~]]	+

OCA at 14:53, 3 November 2021

2021-11-03T14:53:55Z

←Older revision		Revision as of 14:53, 3 November 2021
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	<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [https://pdbe.org/1clw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [https://www.ebi.ac.uk/pdbsum/1clw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [https://pdbe.org/1clw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [https://www.ebi.ac.uk/pdbsum/1clw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bpp22]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Baxa, U]]		[[Category: Baxa, U]]

OCA at 15:17, 3 March 2021

2021-03-03T15:17:26Z

←Older revision		Revision as of 15:17, 3 March 2021
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	<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [~~http~~://pdbe.org/1clw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1clw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [https://pdbe.org/1clw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [https://www.ebi.ac.uk/pdbsum/1clw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TSPE_BPP22 TSPE_BPP22]] Non-covalently bound to the neck of the phage capsid and mediating attachment of the viral particle to host cell-surface polysaccharide. It displays endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.<ref>PMID:12837775</ref> <ref>PMID:20817910</ref>	+	[[https://www.uniprot.org/uniprot/TSPE_BPP22 TSPE_BPP22]] Non-covalently bound to the neck of the phage capsid and mediating attachment of the viral particle to host cell-surface polysaccharide. It displays endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.<ref>PMID:12837775</ref> <ref>PMID:20817910</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 1clw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1clw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tailspike protein\|Tailspike protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:16, 29 May 2019

2019-05-29T07:16:07Z

←Older revision		Revision as of 07:16, 29 May 2019
Line 1:		Line 1:

	==TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT==		==TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT==
-	<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1clw' size='340' side='right'caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Baxa, U]]		[[Category: Baxa, U]]
	[[Category: Huber, R]]		[[Category: Huber, R]]

OCA at 11:23, 24 August 2017

2017-08-24T11:23:00Z

←Older revision		Revision as of 11:23, 24 August 2017
Line 1:		Line 1:
		+
	==TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT==		==TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT==
	<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://pdbe.org/1clw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1clw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://pdbe.org/1clw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bpp22]]
	[[Category: Baxa, U]]		[[Category: Baxa, U]]
	[[Category: Huber, R]]		[[Category: Huber, R]]

OCA at 05:39, 10 September 2015

2015-09-10T05:39:32Z

←Older revision		Revision as of 05:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_p22 Enterobacteria phage p22~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 ~~Enterobacteria phage P22~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 BPP22])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://pdbe.org/1clw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1clw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage p22~~]]	+	[[Category: Bpp22]]
	[[Category: Baxa, U]]		[[Category: Baxa, U]]
	[[Category: Huber, R]]		[[Category: Huber, R]]

OCA at 18:47, 24 December 2014

2014-12-24T18:47:59Z

←Older revision		Revision as of 18:47, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TSPE_BPP22 TSPE_BPP22]] Non-covalently bound to the neck of the phage capsid and mediating attachment of the viral particle to host cell-surface polysaccharide. It displays endorhamnosidase enzymatic activity, hydrolyzing the alpha-1,3-O-glycosidic linkage between rhamnose and galactose of the O-antigen polysaccharide.<ref>PMID:12837775</ref> <ref>PMID:20817910</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 20:27, 22 December 2014

2014-12-22T20:27:17Z

←Older revision		Revision as of 20:27, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
-	[[Category: Baxa, U.]]	+	[[Category: Baxa, U]]
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Seckler, R.]]	+	[[Category: Seckler, R]]
-	[[Category: Steinbacher, S.]]	+	[[Category: Steinbacher, S]]
-	[[Category: Weintraub, A.]]	+	[[Category: Weintraub, A]]
	[[Category: Late protein]]		[[Category: Late protein]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
	[[Category: Virus/viral protein]]		[[Category: Virus/viral protein]]

OCA at 09:27, 27 August 2014

2014-08-27T09:27:45Z

←Older revision		Revision as of 09:27, 27 August 2014
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-	[[~~Image:~~1clw.~~png~~\|~~left\|200px~~]]	+	==TAILSPIKE PROTEIN FROM PHAGE P22, V331A MUTANT==
		+	<StructureSection load='1clw' size='340' side='right' caption='[[1clw]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1clw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CLW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1clw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1clw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1clw RCSB], [http://www.ebi.ac.uk/pdbsum/1clw PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Four previously isolated mutations in Salmonella phage P22 tailspike protein were used to study the relationship between protein stability, folding, and function. Tailspike protein binds and hydrolyzes the repetitive O-antigen structure in Salmonella lipopolysaccharide. Four mutations (V331G, V331A, A334V, A334I) are known to increase the folding efficiency, and two of them (at position 331) also increase the thermal stability of the protein. Octasaccharides comprising two repeating units of the O-antigens from two different Salmonella strains were employed to analyze the receptor binding function of the mutant proteins. Their endorhamnosidase enzymatic activity was assayed with the aid of a fluorescence-labeled dodecasaccharide. Both V331A and V331G were found to strongly affect O-antigen binding. Octasaccharide binding affinities of the mutant proteins are reduced tenfold and 200-fold, corresponding to a loss of 17% and 36% of the standard free energy of binding, respectively. Both mutations at position 334 affected O-antigen binding only slightly (DeltaDeltaG(0)B approximately 1 kJ/mol), but these mutations reduce the thermal stability of the protein. The observed effects on the endoglycosidase activity are fully explained by the changes in substrate binding, suggesting that neither of the mutations affect the catalytic rate. Crystal structures of all four mutants were determined to a resolution of 2.0 A. Except for the partly or completely missing side-chain, no significant changes compared to the wild-type protein structure were found for the mutants at position 331, whereas a small but significant backbone displacement around the mutation site in A334V and A334I may explain the observed thermal destabilization.

-	~~{{STRUCTURE_1clw\| PDB=1clw \| SCENE= }}~~	+	Mutations improving the folding of phage P22 tailspike protein affect its receptor binding activity.,Baxa U, Steinbacher S, Weintraub A, Huber R, Seckler R J Mol Biol. 1999 Oct 29;293(3):693-701. PMID:10543960<ref>PMID:10543960</ref>

-	~~===TAILSPIKE PROTEIN FROM PHAGE P22~~, ~~V331A MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10543960}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1clw]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CLW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010543960<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacteria phage p22]]		[[Category: Enterobacteria phage p22]]
	[[Category: Baxa, U.]]		[[Category: Baxa, U.]]

OCA: Protected "1clw" [edit=sysop:move=sysop]

2012-10-21T20:24:19Z

Protected "1clw" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:24, 21 October 2012