1cqk - Revision history

OCA at 15:41, 13 March 2024

2024-03-13T15:41:51Z

←Older revision		Revision as of 15:41, 13 March 2024
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	<StructureSection load='1cqk' size='340' side='right'caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1cqk' size='340' side='right'caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [https://pdbe.org/1cqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [https://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [https://pdbe.org/1cqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [https://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqk ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The simplest naturally occurring model system for studying immunoglobulin folding and assembly is the non-covalent homodimer formed by the C-terminal domains (CH3) of the heavy chains of IgG. Here, we describe the structure of recombinant CH3 dimer as determined by X-ray crystallography and an analysis of the folding pathway of this protein.Under conditions where prolyl isomerization does not contribute to the folding kinetics, formation of the beta-sandwich structure is the rate-limiting step. beta-Sheet formation of CH3 is a slow process, even compared to other antibody domains, while the subsequent association of the folded monomers is fast. After long-time denaturation, the majority of the unfolded CH3 molecules reaches the native state in two serial reactions, involving the re-isomerization of the Pro35-peptide bond to the cis configuration. The species with the wrong isomer accumulate as a monomeric intermediate. Importantly, the isomerization to the correct cis configuration is the prerequisite for dimerization of the CH3 domain. In contrast, in the Fab fragment of the same antibody, prolyl isomerization occurs after dimerization demonstrating that within one protein, comprised of highly homologous domains, both the kinetics of beta-sandwich formation and the stage at which prolyl isomerization occurs during the folding process can be completely different.
-
-	Folding and association of the antibody domain CH3: prolyl isomerization preceeds dimerization.,Thies MJ, Mayer J, Augustine JG, Frederick CA, Lilie H, Buchner J J Mol Biol. 1999 Oct 15;293(1):67-79. PMID:10512716<ref>PMID:10512716</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 22:
	*[[Sandbox 20009\|Sandbox 20009]]		*[[Sandbox 20009\|Sandbox 20009]]
	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]		*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Augustine~~, J G~~]]	+	[[Category: Augustine JG]]
-	[[Category: Buchner, J]]	+	[[Category: Buchner J]]
-	[[Category: Frederick~~, C A~~]]	+	[[Category: Frederick CA]]
-	[[Category: Lilie, H]]	+	[[Category: Lilie H]]
-	[[Category: Mayer, J]]	+	[[Category: Mayer J]]
-	[[Category: Thies~~, M J]]~~	+	[[Category: Thies MJ]]
-	~~[[Category: C1-subset]]~~	+
-	~~[[Category: Constant domain]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 07:40, 9 March 2022

2022-03-09T07:40:10Z

←Older revision		Revision as of 07:40, 9 March 2022
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	<StructureSection load='1cqk' size='340' side='right'caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1cqk' size='340' side='right'caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1CQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [~~http~~://pdbe.org/1cqk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [https://pdbe.org/1cqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [https://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 28:		Line 28:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Sandbox 20009\|Sandbox 20009]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:49, 3 June 2020

2020-06-03T06:49:54Z

←Older revision		Revision as of 06:49, 3 June 2020
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	==CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY==		==CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY==
-	<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1cqk' size='340' side='right'caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Augustine, J G]]		[[Category: Augustine, J G]]

OCA at 07:42, 29 August 2018

2018-08-29T07:42:15Z

←Older revision		Revision as of 07:42, 29 August 2018
Line 3:		Line 3:
	<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>
	</table>		</table>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Augustine, J G]]		[[Category: Augustine, J G]]
	[[Category: Buchner, J]]		[[Category: Buchner, J]]

OCA at 06:38, 14 March 2018

2018-03-14T06:38:44Z

←Older revision		Revision as of 06:38, 14 March 2018
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:08, 31 January 2018

2018-01-31T06:08:39Z

←Older revision		Revision as of 06:08, 31 January 2018
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:21, 24 August 2017

2017-08-24T11:21:15Z

←Older revision		Revision as of 11:21, 24 August 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY==		==CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY==
	<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:

	==See Also==		==See Also==
-	*[[~~Antibody~~\|~~Antibody~~]]	+	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:12, 11 September 2015

2015-09-11T17:12:11Z

←Older revision		Revision as of 17:12, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://pdbe.org/1cqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cqk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 19:03, 22 December 2014

2014-12-22T19:03:46Z

←Older revision		Revision as of 19:03, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Augustine, J G.]]	+	[[Category: Augustine, J G]]
-	[[Category: Buchner, J.]]	+	[[Category: Buchner, J]]
-	[[Category: Frederick, C A.]]	+	[[Category: Frederick, C A]]
-	[[Category: Lilie, H.]]	+	[[Category: Lilie, H]]
-	[[Category: Mayer, J.]]	+	[[Category: Mayer, J]]
-	[[Category: Thies, M J.]]	+	[[Category: Thies, M J]]
	[[Category: C1-subset]]		[[Category: C1-subset]]
	[[Category: Constant domain]]		[[Category: Constant domain]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]

OCA at 09:25, 27 August 2014

2014-08-27T09:25:41Z

←Older revision		Revision as of 09:25, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cqk.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY==
		+	<StructureSection load='1cqk' size='340' side='right' caption='[[1cqk]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cqk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CQK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cqk RCSB], [http://www.ebi.ac.uk/pdbsum/1cqk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The simplest naturally occurring model system for studying immunoglobulin folding and assembly is the non-covalent homodimer formed by the C-terminal domains (CH3) of the heavy chains of IgG. Here, we describe the structure of recombinant CH3 dimer as determined by X-ray crystallography and an analysis of the folding pathway of this protein.Under conditions where prolyl isomerization does not contribute to the folding kinetics, formation of the beta-sandwich structure is the rate-limiting step. beta-Sheet formation of CH3 is a slow process, even compared to other antibody domains, while the subsequent association of the folded monomers is fast. After long-time denaturation, the majority of the unfolded CH3 molecules reaches the native state in two serial reactions, involving the re-isomerization of the Pro35-peptide bond to the cis configuration. The species with the wrong isomer accumulate as a monomeric intermediate. Importantly, the isomerization to the correct cis configuration is the prerequisite for dimerization of the CH3 domain. In contrast, in the Fab fragment of the same antibody, prolyl isomerization occurs after dimerization demonstrating that within one protein, comprised of highly homologous domains, both the kinetics of beta-sandwich formation and the stage at which prolyl isomerization occurs during the folding process can be completely different.

-	~~{{STRUCTURE_1cqk\| PDB=1cqk \| SCENE= }}~~	+	Folding and association of the antibody domain CH3: prolyl isomerization preceeds dimerization.,Thies MJ, Mayer J, Augustine JG, Frederick CA, Lilie H, Buchner J J Mol Biol. 1999 Oct 15;293(1):67-79. PMID:10512716<ref>PMID:10512716</ref>

-	~~===CRYSTAL STRUCTURE OF THE CH3 DOMAIN FROM THE MAK33 ANTIBODY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10512716}}~~	+	==See Also==
-		+	*[[Antibody\|Antibody]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1cqk~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQK OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010512716<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Augustine, J G.]]		[[Category: Augustine, J G.]]