1crz - Revision history

OCA at 15:42, 13 March 2024

2024-03-13T15:42:27Z

←Older revision		Revision as of 15:42, 13 March 2024
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	<StructureSection load='1crz' size='340' side='right'caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1crz' size='340' side='right'caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [https://pdbe.org/1crz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [https://www.ebi.ac.uk/pdbsum/1crz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [https://pdbe.org/1crz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [https://www.ebi.ac.uk/pdbsum/1crz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI]] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]	+	[https://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: The periplasmic protein TolB from Escherichia coli is part of the Tol-PAL (peptidoglycan-associated lipoprotein) multiprotein complex used by group A colicins to penetrate and kill cells. TolB homologues are found in many gram-negative bacteria and the Tol-PAL system is thought to play a role in bacterial envelope integrity. TolB is required for lethal infection by Salmonella typhimurium in mice. RESULTS: The crystal structure of the selenomethionine-substituted TolB protein from E. coli was solved using multiwavelength anomalous dispersion methods and refined to 1. 95 A. TolB has a two-domain structure. The N-terminal domain consists of two alpha helices, a five-stranded beta-sheet floor and a long loop at the back of this floor. The C-terminal domain is a six-bladed beta propeller. The small, possibly mobile, contact area (430 A(2)) between the two domains involves residues from the two helices and the first and sixth blades of the beta propeller. All available genomic sequences were used to identify new TolB homologues in gram-negative bacteria. The TolB structure was then interpreted using the observed conservation pattern. CONCLUSIONS: The TolB beta-propeller C-terminal domain exhibits sequence similarities to numerous members of the prolyl oligopeptidase family and, to a lesser extent, to class B metallo-beta-lactamases. The alpha/beta N-terminal domain shares a structural similarity with the C-terminal domain of transfer RNA ligases. We suggest that the TolB protein might be part of a multiprotein complex involved in the recycling of peptidoglycan or in its covalent linking with lipoproteins.
-
-	Structure of the Escherichia coli TolB protein determined by MAD methods at 1.95 A resolution.,Abergel C, Bouveret E, Claverie JM, Brown K, Rigal A, Lazdunski C, Benedetti H Structure. 1999 Oct 15;7(10):1291-300. PMID:10545334<ref>PMID:10545334</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1crz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[TolB\|TolB]]		*[[TolB\|TolB]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abergel, C]]	+	[[Category: Abergel C]]
-	[[Category: Benedetti, H]]	+	[[Category: Benedetti H]]
-	[[Category: Bouveret, E]]	+	[[Category: Bouveret E]]
-	[[Category: Brown, K]]	+	[[Category: Brown K]]
-	[[Category: Claverie, J M]]	+	[[Category: Claverie J-M]]
-	[[Category: Lazdunski, C]]	+	[[Category: Lazdunski C]]
-	[[Category: Rigal, A]]	+	[[Category: Rigal A]]
-	~~[[Category: Toxin binding protein]]~~	+
-	~~[[Category: Two domains: beta propeller and alpha/beta fold~~]]	+

OCA at 10:45, 4 August 2021

2021-08-04T10:45:36Z

←Older revision		Revision as of 10:45, 4 August 2021
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	<StructureSection load='1crz' size='340' side='right'caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1crz' size='340' side='right'caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [~~http~~://pdbe.org/1crz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1crz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1crz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [https://pdbe.org/1crz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [https://www.ebi.ac.uk/pdbsum/1crz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI]] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]	+	[[https://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI]] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:53, 30 October 2019

2019-10-30T09:53:51Z

←Older revision		Revision as of 09:53, 30 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN==		==CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN==
-	<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='1crz' size='340' side='right'caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Abergel, C]]		[[Category: Abergel, C]]
	[[Category: Benedetti, H]]		[[Category: Benedetti, H]]

OCA at 18:35, 24 January 2018

2018-01-24T18:35:00Z

←Older revision		Revision as of 18:35, 24 January 2018
Line 40:		Line 40:
	[[Category: Bouveret, E]]		[[Category: Bouveret, E]]
	[[Category: Brown, K]]		[[Category: Brown, K]]
-	[[Category: Claverie, J-M]]	+	[[Category: Claverie, J M]]
	[[Category: Lazdunski, C]]		[[Category: Lazdunski, C]]
	[[Category: Rigal, A]]		[[Category: Rigal, A]]
	[[Category: Toxin binding protein]]		[[Category: Toxin binding protein]]
	[[Category: Two domains: beta propeller and alpha/beta fold]]		[[Category: Two domains: beta propeller and alpha/beta fold]]

OCA at 08:19, 6 December 2017

2017-12-06T08:19:53Z

←Older revision		Revision as of 08:19, 6 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN==		==CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN==
	<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://pdbe.org/1crz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://pdbe.org/1crz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1crz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:27, 8 February 2016

2016-02-08T19:27:20Z

←Older revision		Revision as of 19:27, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:40, 10 September 2015

2015-09-10T14:40:46Z

←Older revision		Revision as of 14:40, 10 September 2015
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	<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://pdbe.org/1crz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1crz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Abergel, C]]		[[Category: Abergel, C]]
	[[Category: Benedetti, H]]		[[Category: Benedetti, H]]

OCA at 01:38, 25 December 2014

2014-12-25T01:38:26Z

←Older revision		Revision as of 01:38, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI]] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:23, 22 December 2014

2014-12-22T23:23:08Z

←Older revision		Revision as of 23:23, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Abergel, C.]]	+	[[Category: Abergel, C]]
-	[[Category: Benedetti, H.]]	+	[[Category: Benedetti, H]]
-	[[Category: Bouveret, E.]]	+	[[Category: Bouveret, E]]
-	[[Category: Brown, K.]]	+	[[Category: Brown, K]]
-	[[Category: Claverie, J-M.]]	+	[[Category: Claverie, J-M]]
-	[[Category: Lazdunski, C.]]	+	[[Category: Lazdunski, C]]
-	[[Category: Rigal, A.]]	+	[[Category: Rigal, A]]
	[[Category: Toxin binding protein]]		[[Category: Toxin binding protein]]
	[[Category: Two domains: beta propeller and alpha/beta fold]]		[[Category: Two domains: beta propeller and alpha/beta fold]]

OCA at 09:10, 27 August 2014

2014-08-27T09:10:42Z

←Older revision		Revision as of 09:10, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1crz.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE E. COLI TOLB PROTEIN==
		+	<StructureSection load='1crz' size='340' side='right' caption='[[1crz]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1crz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CRZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1crz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1crz RCSB], [http://www.ebi.ac.uk/pdbsum/1crz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The periplasmic protein TolB from Escherichia coli is part of the Tol-PAL (peptidoglycan-associated lipoprotein) multiprotein complex used by group A colicins to penetrate and kill cells. TolB homologues are found in many gram-negative bacteria and the Tol-PAL system is thought to play a role in bacterial envelope integrity. TolB is required for lethal infection by Salmonella typhimurium in mice. RESULTS: The crystal structure of the selenomethionine-substituted TolB protein from E. coli was solved using multiwavelength anomalous dispersion methods and refined to 1. 95 A. TolB has a two-domain structure. The N-terminal domain consists of two alpha helices, a five-stranded beta-sheet floor and a long loop at the back of this floor. The C-terminal domain is a six-bladed beta propeller. The small, possibly mobile, contact area (430 A(2)) between the two domains involves residues from the two helices and the first and sixth blades of the beta propeller. All available genomic sequences were used to identify new TolB homologues in gram-negative bacteria. The TolB structure was then interpreted using the observed conservation pattern. CONCLUSIONS: The TolB beta-propeller C-terminal domain exhibits sequence similarities to numerous members of the prolyl oligopeptidase family and, to a lesser extent, to class B metallo-beta-lactamases. The alpha/beta N-terminal domain shares a structural similarity with the C-terminal domain of transfer RNA ligases. We suggest that the TolB protein might be part of a multiprotein complex involved in the recycling of peptidoglycan or in its covalent linking with lipoproteins.

-	~~{{STRUCTURE_1crz\| PDB=1crz \| SCENE= }}~~	+	Structure of the Escherichia coli TolB protein determined by MAD methods at 1.95 A resolution.,Abergel C, Bouveret E, Claverie JM, Brown K, Rigal A, Lazdunski C, Benedetti H Structure. 1999 Oct 15;7(10):1291-300. PMID:10545334<ref>PMID:10545334</ref>

-	~~===CRYSTAL STRUCTURE OF THE E~~. ~~COLI TOLB PROTEIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10545334}}~~	+	==See Also==
-		+	*[[TolB\|TolB]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1crz~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRZ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010545334<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Abergel, C.]]		[[Category: Abergel, C.]]