1ct4 - Revision history

OCA at 15:42, 13 March 2024

2024-03-13T15:42:53Z

←Older revision		Revision as of 15:42, 13 March 2024
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	<StructureSection load='1ct4' size='340' side='right'caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1ct4' size='340' side='right'caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Melga Melga~~] and [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3sgb\|3sgb]]~~, [[~~1sgr~~\|~~1sgr~~]]~~, [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en~~.~~wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [https://pdbe.org/1ct4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ct4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [https://pdbe.org/1ct4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ct4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids.	+	[https://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR] Has a primary specificity for large aliphatic or aromatic amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ct4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ct4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Turkey ovomucoid third domain (OMTKY3) is a canonical inhibitor of serine proteinases. Upon complex formation, the inhibitors fully exposed P1 residue becomes fully buried in the preformed cavity of the enzyme. All 20 P1 variants of OMTKY3 have been obtained by recombinant DNA technology and their equilibrium association constants have been measured with six serine proteinases. To rationalize the trends observed in this data set, high resolution crystal structures have been determined for OMTKY3 P1 variants in complex with the bacterial serine proteinase, Streptomyces griseus proteinase B (SGPB). Four high resolution complex structures are being reported in this paper; the three beta-branched variants, Ile18I, Val18I, and Thr18I, determined to 2.1, 1.6, and 1.7 A resolution, respectively, and the structure of the Ser18I variant complex, determined to 1.9 A resolution. Models of the Cys18I, Hse18I, and Ape18I variant complexes are also discussed. The beta-branched side chains are not complementary to the shape of the S1 binding pocket in SGPB, in contrast to that of the wild-type gamma-branched P1 residue for OMTKY3, Leu18I. Chi1 angles of approximately 40 degrees are imposed on the side chains of Ile18I, Val18I, and Thr18I within the S1 pocket. Dihedral angles of +60 degrees, -60 degrees, or 180 degrees are more commonly observed but 40 degrees is not unfavorable for the beta-branched side chains. Thr18I Ogamma1 also forms a hydrogen bond with Ser195 Ogamma in this orientation. The Ser18I side chain adopts two alternate conformations within the S1 pocket of SGPB, suggesting that the side chain is not stable in either conformation.
-
-	Deleterious effects of beta-branched residues in the S1 specificity pocket of Streptomyces griseus proteinase B (SGPB): crystal structures of the turkey ovomucoid third domain variants Ile18I, Val18I, Thr18I, and Ser18I in complex with SGPB.,Bateman KS, Anderson S, Lu W, Qasim MA, Laskowski M Jr, James MN Protein Sci. 2000 Jan;9(1):83-94. PMID:10739250<ref>PMID:10739250</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ct4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Proteinase\|Proteinase]]
	*[[Proteinase 3D structures\|Proteinase 3D structures]]		*[[Proteinase 3D structures\|Proteinase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Melga]]~~	+	[[Category: Meleagris gallopavo]]
-	~~[[Category: Streptogrisin B~~]]	+
	[[Category: Streptomyces griseus]]		[[Category: Streptomyces griseus]]
-	[[Category: Anderson, S]]	+	[[Category: Anderson S]]
-	[[Category: Bateman~~, K S~~]]	+	[[Category: Bateman KS]]
-	[[Category: James~~, M N.G~~]]	+	[[Category: James MNG]]
-	[[Category: Laskowski, M]]	+	[[Category: Laskowski Jr M]]
-	[[Category: Lu, W]]	+	[[Category: Lu W]]
-	[[Category: Qasim~~, M A]]~~	+	[[Category: Qasim MA]]
-	~~[[Category: Beta-branched p1 residue]]~~	+
-	~~[[Category: Enzyme-inhibitor complex]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex~~]]	+

OCA at 10:39, 14 July 2021

2021-07-14T10:39:59Z

←Older revision		Revision as of 10:39, 14 July 2021
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	<StructureSection load='1ct4' size='340' side='right'caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1ct4' size='340' side='right'caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Melga Melga] and [~~http~~://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Melga Melga] and [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [~~http~~://pdbe.org/1ct4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ct4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [https://pdbe.org/1ct4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ct4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids.	+	[[https://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	==See Also==		==See Also==
	*[[Proteinase\|Proteinase]]		*[[Proteinase\|Proteinase]]
		+	*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:40, 10 October 2019

2019-10-10T06:40:22Z

←Older revision		Revision as of 06:40, 10 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB==		==CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB==
-	<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1ct4' size='340' side='right'caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Melga Melga] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Melga Melga] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Melga]]		[[Category: Melga]]
	[[Category: Streptogrisin B]]		[[Category: Streptogrisin B]]

OCA at 08:21, 6 December 2017

2017-12-06T08:21:23Z

←Older revision		Revision as of 08:21, 6 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB==		==CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB==
	<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914~~] and [http://en.wikipedia.org/wiki/~~Common_turkey Common turkey~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Melga Melga] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://pdbe.org/1ct4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://pdbe.org/1ct4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ct4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/1ct4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/1ct4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actinomyces griseus krainsky 1914]]~~	+	[[Category: Melga]]
-	~~[[Category: Common turkey~~]]	+
	[[Category: Streptogrisin B]]		[[Category: Streptogrisin B]]
		+	[[Category: Streptomyces griseus]]
	[[Category: Anderson, S]]		[[Category: Anderson, S]]
	[[Category: Bateman, K S]]		[[Category: Bateman, K S]]

OCA at 16:05, 8 February 2016

2016-02-08T16:05:48Z

←Older revision		Revision as of 16:05, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ct4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:13, 10 September 2015

2015-09-10T13:13:35Z

←Older revision		Revision as of 13:13, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Meleagris_gallopavo Meleagris gallopavo~~] and [http://en.wikipedia.org/wiki/~~Streptomyces_griseus Streptomyces griseus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914] and [http://en.wikipedia.org/wiki/Common_turkey Common turkey]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://pdbe.org/1ct4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ct4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Meleagris gallopavo~~]]	+	[[Category: Actinomyces griseus krainsky 1914]]
		+	[[Category: Common turkey]]
	[[Category: Streptogrisin B]]		[[Category: Streptogrisin B]]
-	[[Category: Streptomyces griseus]]
	[[Category: Anderson, S]]		[[Category: Anderson, S]]
	[[Category: Bateman, K S]]		[[Category: Bateman, K S]]

OCA at 00:31, 25 December 2014

2014-12-25T00:31:29Z

←Older revision		Revision as of 00:31, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 21:48, 22 December 2014

2014-12-22T21:48:31Z

←Older revision		Revision as of 21:48, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Streptogrisin B]]		[[Category: Streptogrisin B]]
	[[Category: Streptomyces griseus]]		[[Category: Streptomyces griseus]]
-	[[Category: Anderson, S.]]	+	[[Category: Anderson, S]]
-	[[Category: Bateman, K S.]]	+	[[Category: Bateman, K S]]
-	[[Category: James, M N.G.]]	+	[[Category: James, M N.G]]
-	[[Category: Laskowski, M.]]	+	[[Category: Laskowski, M]]
-	[[Category: Lu, W.]]	+	[[Category: Lu, W]]
-	[[Category: Qasim, M A.]]	+	[[Category: Qasim, M A]]
	[[Category: Beta-branched p1 residue]]		[[Category: Beta-branched p1 residue]]
	[[Category: Enzyme-inhibitor complex]]		[[Category: Enzyme-inhibitor complex]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 10:12, 3 October 2014

2014-10-03T10:12:51Z

←Older revision		Revision as of 10:12, 3 October 2014
Line 28:		Line 28:
	==See Also==		==See Also==
	*[[Proteinase\|Proteinase]]		*[[Proteinase\|Proteinase]]
-	*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:12, 27 August 2014

2014-08-27T09:12:14Z

←Older revision		Revision as of 09:12, 27 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1ct4.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB==
		+	<StructureSection load='1ct4' size='340' side='right' caption='[[1ct4]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ct4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CT4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sgb\|3sgb]], [[1sgr\|1sgr]], [[1sgq\|1sgq]], [[1sgp\|1sgp]], [[1cso\|1cso]], [[1ct0\|1ct0]], [[1ct2\|1ct2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptogrisin_B Streptogrisin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.81 3.4.21.81] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ct4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ct4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/1ct4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Turkey ovomucoid third domain (OMTKY3) is a canonical inhibitor of serine proteinases. Upon complex formation, the inhibitors fully exposed P1 residue becomes fully buried in the preformed cavity of the enzyme. All 20 P1 variants of OMTKY3 have been obtained by recombinant DNA technology and their equilibrium association constants have been measured with six serine proteinases. To rationalize the trends observed in this data set, high resolution crystal structures have been determined for OMTKY3 P1 variants in complex with the bacterial serine proteinase, Streptomyces griseus proteinase B (SGPB). Four high resolution complex structures are being reported in this paper; the three beta-branched variants, Ile18I, Val18I, and Thr18I, determined to 2.1, 1.6, and 1.7 A resolution, respectively, and the structure of the Ser18I variant complex, determined to 1.9 A resolution. Models of the Cys18I, Hse18I, and Ape18I variant complexes are also discussed. The beta-branched side chains are not complementary to the shape of the S1 binding pocket in SGPB, in contrast to that of the wild-type gamma-branched P1 residue for OMTKY3, Leu18I. Chi1 angles of approximately 40 degrees are imposed on the side chains of Ile18I, Val18I, and Thr18I within the S1 pocket. Dihedral angles of +60 degrees, -60 degrees, or 180 degrees are more commonly observed but 40 degrees is not unfavorable for the beta-branched side chains. Thr18I Ogamma1 also forms a hydrogen bond with Ser195 Ogamma in this orientation. The Ser18I side chain adopts two alternate conformations within the S1 pocket of SGPB, suggesting that the side chain is not stable in either conformation.

-	~~{{STRUCTURE_1ct4\| PDB=1ct4 \| SCENE= }}~~	+	Deleterious effects of beta-branched residues in the S1 specificity pocket of Streptomyces griseus proteinase B (SGPB): crystal structures of the turkey ovomucoid third domain variants Ile18I, Val18I, Thr18I, and Ser18I in complex with SGPB.,Bateman KS, Anderson S, Lu W, Qasim MA, Laskowski M Jr, James MN Protein Sci. 2000 Jan;9(1):83-94. PMID:10739250<ref>PMID:10739250</ref>

-	~~===CRYSTAL STRUCTURE OF THE OMTKY3 P1 VARIANT OMTKY3-VAL18I IN COMPLEX WITH SGPB===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10739250}}~~	+	==See Also==
-		+	*[[Proteinase\|Proteinase]]
-	==~~About this Structure~~==	+	*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]
-	[[~~1ct4~~]] ~~is a 2 chain structure with sequence from~~ [~~http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] and~~ [~~http://en.wikipedia.org/wiki/Streptomyces_griseus~~ Streptomyces griseus]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CT4 OCA~~].	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:010739250<~~/~~ref~~><~~references group="xtra"~~/>	+	</StructureSection>
	[[Category: Meleagris gallopavo]]		[[Category: Meleagris gallopavo]]
	[[Category: Streptogrisin B]]		[[Category: Streptogrisin B]]