1cug - Revision history

OCA at 15:43, 13 March 2024

2024-03-13T15:43:26Z

←Older revision		Revision as of 15:43, 13 March 2024
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	<StructureSection load='1cug' size='340' side='right'caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1cug' size='340' side='right'caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_vanettenii Fusarium vanettenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~1.~~1.3 3.1.1.3] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [https://pdbe.org/1cug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [https://www.ebi.ac.uk/pdbsum/1cug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [https://pdbe.org/1cug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [https://www.ebi.ac.uk/pdbsum/1cug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CUTI1_FUSSO CUTI1_FUSSO]~~] Catalyzes the hydrolysis of cutin, a ~~polyester~~ that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of ~~the~~ fungal infection.	+	[https://www.uniprot.org/uniprot/CUTI1_FUSVN CUTI1_FUSVN] Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (PubMed:18658138, PubMed:8286366, PubMed:8555209, PubMed:19810726). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (PubMed:18658138, PubMed:8286366, PubMed:8555209, PubMed:19810726). Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of fungal infection (Ref.4).<ref>PMID:18658138</ref> <ref>PMID:19810726</ref> <ref>PMID:8286366</ref> <ref>PMID:8555209</ref> [PROSITE-ProRule:PRU10109]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cug ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cug ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In characterizing mutants and covalently inhibited complexes of Fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. Taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. Surface loops were identified as the major flexible protein regions, particularly those forming the active-site groove, whereas the elements constituting the protein scaffold were found to retain the same conformation in all the cutinase variants studied. Flexibility turned out to be correlated with thermal motion. With a given crystal packing environment, a high flexibility turned out to be correlated with a low involvement in crystal packing contacts. The high degree of crystal polymorphism, which allowed different conformations with similar energy to be detected, made it possible to identify motions which would have remained unidentified if only a single crystal form had been available. Fairly good agreement was found to exist between the data obtained from the structural comparison and those from a molecular dynamics (MD) simulation carried out on the native enzyme. The crystallographic approach used in this study turned out to be a suitable tool for investigating cutinase dynamics. Because of the availability of a set of closely related proteins in different crystal environments, the intrinsic drawback of a crystallographic approach was bypassed. By combining several static pictures, the dynamics of the protein could be monitored much more realistically than what can be achieved on the basis of static pictures alone.
-
-	Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants.,Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C Proteins. 1996 Dec;26(4):442-58. PMID:8990497<ref>PMID:8990497</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cug" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium vanettenii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Triacylglycerol lipase]]~~	+	[[Category: Cambillau C]]
-	[[Category: Cambillau, C]]	+	[[Category: Longhi S]]
-	[[Category: Longhi, S]]	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine esterase~~]]	+

OCA at 15:19, 3 March 2021

2021-03-03T15:19:03Z

←Older revision		Revision as of 15:19, 3 March 2021
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	<StructureSection load='1cug' size='340' side='right'caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1cug' size='340' side='right'caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [~~http~~://pdbe.org/1cug PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cug PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cug ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [https://pdbe.org/1cug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [https://www.ebi.ac.uk/pdbsum/1cug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.	+	[[https://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:34, 29 May 2019

2019-05-29T07:34:15Z

←Older revision		Revision as of 07:34, 29 May 2019
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	==CUTINASE, R17E, N172K MUTANT==		==CUTINASE, R17E, N172K MUTANT==
-	<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='1cug' size='340' side='right'caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cug_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cug_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Cutinase\|Cutinase]]	+	*[[Cutinase 3D structures\|Cutinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]

OCA at 11:17, 24 August 2017

2017-08-24T11:17:17Z

←Older revision		Revision as of 11:17, 24 August 2017
Line 1:		Line 1:
		+
	==CUTINASE, R17E, N172K MUTANT==		==CUTINASE, R17E, N172K MUTANT==
	<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://pdbe.org/1cug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://pdbe.org/1cug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cug ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fusso]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]

OCA at 10:14, 11 September 2015

2015-09-11T10:14:12Z

←Older revision		Revision as of 10:14, 11 September 2015
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	<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nectria_haematococca Nectria haematococca~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://pdbe.org/1cug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cug" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Nectria haematococca~~]]	+	[[Category: Fusso]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]

OCA at 15:01, 25 December 2014

2014-12-25T15:01:03Z

←Older revision		Revision as of 15:01, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 00:40, 23 December 2014

2014-12-23T00:40:20Z

←Older revision		Revision as of 00:40, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca Nectria haematococca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca Nectria haematococca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Nectria haematococca]]		[[Category: Nectria haematococca]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
-	[[Category: Cambillau, C.]]	+	[[Category: Cambillau, C]]
-	[[Category: Longhi, S.]]	+	[[Category: Longhi, S]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine esterase]]		[[Category: Serine esterase]]

OCA at 09:21, 27 August 2014

2014-08-27T09:21:04Z

←Older revision		Revision as of 09:21, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cug.~~png\|left~~\|200px]]	+	==CUTINASE, R17E, N172K MUTANT==
		+	<StructureSection load='1cug' size='340' side='right' caption='[[1cug]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca Nectria haematococca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cug RCSB], [http://www.ebi.ac.uk/pdbsum/1cug PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cug_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In characterizing mutants and covalently inhibited complexes of Fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. Taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. Surface loops were identified as the major flexible protein regions, particularly those forming the active-site groove, whereas the elements constituting the protein scaffold were found to retain the same conformation in all the cutinase variants studied. Flexibility turned out to be correlated with thermal motion. With a given crystal packing environment, a high flexibility turned out to be correlated with a low involvement in crystal packing contacts. The high degree of crystal polymorphism, which allowed different conformations with similar energy to be detected, made it possible to identify motions which would have remained unidentified if only a single crystal form had been available. Fairly good agreement was found to exist between the data obtained from the structural comparison and those from a molecular dynamics (MD) simulation carried out on the native enzyme. The crystallographic approach used in this study turned out to be a suitable tool for investigating cutinase dynamics. Because of the availability of a set of closely related proteins in different crystal environments, the intrinsic drawback of a crystallographic approach was bypassed. By combining several static pictures, the dynamics of the protein could be monitored much more realistically than what can be achieved on the basis of static pictures alone.

-	~~{{STRUCTURE_1cug\| PDB=1cug \| SCENE= }}~~	+	Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants.,Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C Proteins. 1996 Dec;26(4):442-58. PMID:8990497<ref>PMID:8990497</ref>

-	~~===CUTINASE~~, ~~R17E, N172K MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_8990497}}~~	+	==See Also==
-		+	*[[Cutinase\|Cutinase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1cug~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca Nectria haematococca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:008990497<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Nectria haematococca]]		[[Category: Nectria haematococca]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]

OCA: Protected "1cug" [edit=sysop:move=sysop]

2012-10-21T20:44:44Z

Protected "1cug" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:44, 21 October 2012

OCA at 20:44, 21 October 2012

2012-10-21T20:44:40Z

←Older revision		Revision as of 20:44, 21 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1cug.png\|left\|200px]]		[[Image:1cug.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1cug", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1cug\| PDB=1cug \| SCENE= }}		{{STRUCTURE_1cug\| PDB=1cug \| SCENE= }}

	===CUTINASE, R17E, N172K MUTANT===		===CUTINASE, R17E, N172K MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8990497}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8990497 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_8990497}}		{{ABSTRACT_PUBMED_8990497}}

	==About this Structure==		==About this Structure==
-	~~1CUG~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA].	+	[[1cug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca Nectria haematococca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUG OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8990497~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008990497</ref><references group="xtra"/>
-	[[Category: ~~Fusarium solani subsp. pisi~~]]	+	[[Category: Nectria haematococca]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C.]]		[[Category: Cambillau, C.]]
Line 31:		Line 19:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine esterase]]		[[Category: Serine esterase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:40:38 2009''