1d0u - Revision history

OCA at 11:42, 22 November 2023

2023-11-22T11:42:18Z

←Older revision		Revision as of 11:42, 22 November 2023
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	==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==		==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==
-	<StructureSection load='1d0u' size='340' side='right'caption='[[1d0u~~]], [[NMR_Ensembles_of_Models \| 23 NMR models~~]]' scene=''>	+	<StructureSection load='1d0u' size='340' side='right'caption='[[1d0u]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1d0t\|1d0t]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [https://pdbe.org/1d0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [https://www.ebi.ac.uk/pdbsum/1d0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [https://pdbe.org/1d0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [https://www.ebi.ac.uk/pdbsum/1d0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0u ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nikonowicz~~, E P~~]]	+	[[Category: Nikonowicz EP]]
-	[[Category: Smith~~, J S]]~~	+	[[Category: Smith JS]]
-	~~[[Category: Bulged base]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rna hairpin]]~~	+
-	~~[[Category: Stem-loop~~]]	+

OCA at 15:20, 3 March 2021

2021-03-03T15:20:53Z

←Older revision		Revision as of 15:20, 3 March 2021
Line 3:		Line 3:
	<StructureSection load='1d0u' size='340' side='right'caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>		<StructureSection load='1d0u' size='340' side='right'caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1D0U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d0t\|1d0t]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [~~http~~://pdbe.org/1d0u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1d0u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [https://pdbe.org/1d0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [https://www.ebi.ac.uk/pdbsum/1d0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0u ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:42, 29 May 2019

2019-05-29T07:42:08Z

←Older revision		Revision as of 07:42, 29 May 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==		==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==
-	<StructureSection load='1d0u' size='340' side='right' caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>	+	<StructureSection load='1d0u' size='340' side='right'caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Nikonowicz, E P]]		[[Category: Nikonowicz, E P]]
	[[Category: Smith, J S]]		[[Category: Smith, J S]]

OCA at 11:21, 24 August 2017

2017-08-24T11:21:47Z

←Older revision		Revision as of 11:21, 24 August 2017
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==		==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==
	<StructureSection load='1d0u' size='340' side='right' caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>		<StructureSection load='1d0u' size='340' side='right' caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://pdbe.org/1d0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://pdbe.org/1d0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0u ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:58, 10 September 2015

2015-09-10T02:58:46Z

←Older revision		Revision as of 02:58, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://pdbe.org/1d0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1d0u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:02, 23 December 2014

2014-12-23T00:02:20Z

←Older revision		Revision as of 00:02, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Nikonowicz, E P.]]	+	[[Category: Nikonowicz, E P]]
-	[[Category: Smith, J S.]]	+	[[Category: Smith, J S]]
	[[Category: Bulged base]]		[[Category: Bulged base]]
	[[Category: Rna]]		[[Category: Rna]]
	[[Category: Rna hairpin]]		[[Category: Rna hairpin]]
	[[Category: Stem-loop]]		[[Category: Stem-loop]]

OCA at 09:26, 27 August 2014

2014-08-27T09:26:18Z

←Older revision		Revision as of 09:26, 27 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1d0u.~~png~~\|~~left~~\|~~200px~~]]	+	==SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN==
		+	<StructureSection load='1d0u' size='340' side='right' caption='[[1d0u]], [[NMR_Ensembles_of_Models \| 23 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D0U FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d0t\|1d0t]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1d0u RCSB], [http://www.ebi.ac.uk/pdbsum/1d0u PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphorothioate substitution-interference experiments, routinely used to stereospecifically identify phosphoryl oxygen sites that participate in RNA-ligand binding and RNA-directed catalysis, rest in their interpretation on the untested assumption that substitution does not alter the conformation of the modified molecule from its biologically active state. Using NMR spectroscopy, we have tested this assumption by determining the structural effect of stereospecific phosphorothioate substitution at five positions in an RNA hairpin containing the binding site for bacteriophage MS2 capsid protein. At most sites, substitution has little or no effect, causing minor perturbations in the phosphate backbone and increasing the stacking among nucleotides in the hairpin loop. At one site, however, phosphorothioate substitution causes an unpaired adenine necessary for formation of the capsid protein-RNA complex to loop out of the RNA helix into the major groove. These results indicate that phosphorothioate substitution can substantially alter the conformation of RNA at positions of irregular secondary structure, complicating the use of substitution-interference experiments to study RNA structure and function.

-	~~{{STRUCTURE_1d0u\| PDB=1d0u \| SCENE= }}~~	+	Phosphorothioate substitution can substantially alter RNA conformation.,Smith JS, Nikonowicz EP Biochemistry. 2000 May 16;39(19):5642-52. PMID:10801314<ref>PMID:10801314</ref>

-	~~===SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10801314}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1d0u]] is a 1 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA].~~	+	</StructureSection>
	[[Category: Nikonowicz, E P.]]		[[Category: Nikonowicz, E P.]]
	[[Category: Smith, J S.]]		[[Category: Smith, J S.]]

OCA: Protected "1d0u" [edit=sysop:move=sysop]

2012-10-21T20:52:47Z

Protected "1d0u" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:52, 21 October 2012

OCA at 20:52, 21 October 2012

2012-10-21T20:52:45Z

←Older revision		Revision as of 20:52, 21 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1d0u.png\|left\|200px]]		[[Image:1d0u.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1d0u", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1d0u\| PDB=1d0u \| SCENE= }}		{{STRUCTURE_1d0u\| PDB=1d0u \| SCENE= }}

	===SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN===		===SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10801314}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10801314 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10801314}}		{{ABSTRACT_PUBMED_10801314}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA].	+	[[1d0u]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA].
-		+
-	~~==Reference==~~	+
-	~~Phosphorothioate substitution can substantially alter RNA conformation., Smith JS, Nikonowicz EP, Biochemistry. 2000 May 16;39(19):5642-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10801314 10801314]~~	+
	[[Category: Nikonowicz, E P.]]		[[Category: Nikonowicz, E P.]]
	[[Category: Smith, J S.]]		[[Category: Smith, J S.]]
	[[Category: Bulged base]]		[[Category: Bulged base]]
		+	[[Category: Rna]]
	[[Category: Rna hairpin]]		[[Category: Rna hairpin]]
	[[Category: Stem-loop]]		[[Category: Stem-loop]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:58:52 2008''

OCA at 18:59, 30 June 2008

2008-06-30T18:59:03Z

←Older revision		Revision as of 18:59, 30 June 2008
Line 1:		Line 1:
-	[[Image:1d0u.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1d0u.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1d0u\| PDB=1d0u \| SCENE= }}		{{STRUCTURE_1d0u\| PDB=1d0u \| SCENE= }}

-	~~'''~~SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN~~'''~~	+	===SOLUTION STRUCTURE OF AN RNA BINDING SITE FOR PHAGE MS2 COAT PROTEIN===


-	~~==Overview==~~	+	<!--
-	~~Phosphorothioate substitution~~-~~interference experiments, routinely used to stereospecifically identify phosphoryl oxygen sites that participate in RNA~~-ligand binding and RNA-directed catalysis, rest in their interpretation on the untested assumption that substitution does not alter the conformation of the modified molecule from its biologically active state. Using NMR spectroscopy, we have tested this assumption by determining the structural effect of stereospecific phosphorothioate substitution at five positions in an RNA hairpin containing the binding site for bacteriophage MS2 capsid protein. At most sites, ~~substitution has little or no effect~~, ~~causing minor perturbations in~~ the ~~phosphate backbone and increasing~~ the ~~stacking among nucleotides in the hairpin loop~~. ~~At one site~~, ~~however, phosphorothioate substitution causes an unpaired adenine necessary for formation of~~ the ~~capsid protein-RNA complex to loop out of the RNA helix into the major groove~~. ~~These results indicate that phosphorothioate substitution can substantially alter the conformation of RNA at positions of irregular secondary structure, complicating the use of substitution~~-~~interference experiments to study RNA structure and function.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_10801314}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10801314 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10801314}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0U OCA].

	==Reference==		==Reference==
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	[[Category: Rna hairpin]]		[[Category: Rna hairpin]]
	[[Category: Stem-loop]]		[[Category: Stem-loop]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 13~~:19:21 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:58:52 2008''