1deu - Revision history

OCA at 05:56, 9 August 2023

2023-08-09T05:56:44Z

←Older revision		Revision as of 05:56, 9 August 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [https://pdbe.org/1deu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [https://www.ebi.ac.uk/pdbsum/1deu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1deu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [https://pdbe.org/1deu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [https://www.ebi.ac.uk/pdbsum/1deu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1deu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CATZ_HUMAN CATZ_HUMAN]] Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.	+	[https://www.uniprot.org/uniprot/CATZ_HUMAN CATZ_HUMAN] Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cygler, M]]	+	[[Category: Cygler M]]
-	[[Category: Menard, R]]	+	[[Category: Menard R]]
-	[[Category: Nagler~~, D K~~]]	+	[[Category: Nagler DK]]
-	[[Category: Sivaraman, J]]	+	[[Category: Sivaraman J]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Cysteine protease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Procathepsin x]]~~	+
-	~~[[Category: Proregion]]~~	+
-	~~[[Category: Prosegment~~]]	+

OCA at 18:26, 10 March 2021

2021-03-10T18:26:00Z

←Older revision		Revision as of 18:26, 10 March 2021
Line 3:		Line 3:
	<StructureSection load='1deu' size='340' side='right'caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1deu' size='340' side='right'caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DEU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [~~http~~://pdbe.org/1deu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1deu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1deu ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [https://pdbe.org/1deu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [https://www.ebi.ac.uk/pdbsum/1deu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1deu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CATZ_HUMAN CATZ_HUMAN]] Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.	+	[[https://www.uniprot.org/uniprot/CATZ_HUMAN CATZ_HUMAN]] Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:42, 5 June 2019

2019-06-05T23:42:31Z

←Older revision		Revision as of 23:42, 5 June 2019
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	==CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE==		==CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE==
-	<StructureSection load='1deu' size='340' side='right' caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1deu' size='340' side='right'caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/de/1deu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/de/1deu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Cathepsin\|Cathepsin]]	+	*[[Cathepsin 3D structures\|Cathepsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]
	[[Category: Menard, R]]		[[Category: Menard, R]]

OCA at 04:12, 30 August 2017

2017-08-30T04:12:32Z

←Older revision		Revision as of 04:12, 30 August 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE==		==CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE==
	<StructureSection load='1deu' size='340' side='right' caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1deu' size='340' side='right' caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://pdbe.org/1deu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://pdbe.org/1deu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1deu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1deu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:56, 11 September 2015

2015-09-11T21:56:31Z

←Older revision		Revision as of 21:56, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://pdbe.org/1deu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1deu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 20:06, 25 December 2014

2014-12-25T20:06:57Z

←Older revision		Revision as of 20:06, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CATZ_HUMAN CATZ_HUMAN]] Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 20:20, 22 December 2014

2014-12-22T20:20:08Z

←Older revision		Revision as of 20:20, 22 December 2014
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Cygler, M.]]	+	[[Category: Cygler, M]]
-	[[Category: Menard, R.]]	+	[[Category: Menard, R]]
-	[[Category: Nagler, D K.]]	+	[[Category: Nagler, D K]]
-	[[Category: Sivaraman, J.]]	+	[[Category: Sivaraman, J]]
-	[[Category: Zhang, R.]]	+	[[Category: Zhang, R]]
	[[Category: Cysteine protease]]		[[Category: Cysteine protease]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:58, 10 October 2014

2014-10-10T05:58:48Z

←Older revision		Revision as of 05:58, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:42, 4 September 2014

2014-09-04T06:42:30Z

←Older revision		Revision as of 06:42, 4 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1deu.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE==
		+	<StructureSection load='1deu' size='340' side='right' caption='[[1deu]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DEU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1deu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1deu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1deu RCSB], [http://www.ebi.ac.uk/pdbsum/1deu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/de/1deu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human cathepsin X is one of many proteins discovered in recent years through the mining of sequence databases. Its sequence shows clear homology to cysteine proteases from the papain family, containing the characteristic residue patterns, including the active site. However, the proregion of cathepsin X is only 38 residues long, the shortest among papain-like enzymes, and the cathepsin X sequence has an atypical insertion in the regions proximal to the active site. This protein was recently expressed and partially characterized biochemically. Unlike most other cysteine proteases from the papain family, procathepsin X is incapable of autoprocessing in vitro but can be processed under reducing conditions by exogenous cathepsin L. Atypically, the mature enzyme is primarily a carboxypeptidase and has extremely poor endopeptidase activity. We have determined the three-dimensional structure of the procathepsin X at 1.7 A resolution. The overall structure of the mature enzyme is characteristic for enzymes of the papain superfamily, but contains several novel features. Most interestingly, the short proregion binds to the enzyme with the aid of a covalent bond between the cysteine residue in the proregion (Cys10p) and the active site cysteine residue (Cys31). This is the first example of a zymogen in which the inhibition of enzyme's proteolytic activity by the proregion is achieved through a reversible covalent modification of the active site nucleophile. Such mode of binding requires less contact area between the proregion and the enzyme than observed in other procathepsins, and no auxiliary binding site on the enzyme surface is used. A three-residue insertion in a highly conserved region, just prior to the active site cysteine residue, confers a significantly different shape on the S' subsites, compared to other proteases from papain family. The 3D structure provides an explanation for the rather unusual carboxypeptidase activity of this enzyme and confirms the predictions based on homology modeling. Another long insertion in the cathepsin X amino acid sequence forms a beta-hairpin pointing away from the active site. This insertion, thought to be an equivalent of cathepsin B occluding loop, is located on the side of the protein, distant from the substrate binding site.

-	~~{{STRUCTURE_1deu\| PDB=1deu \| SCENE= }}~~	+	Crystal structure of human procathepsin X: a cysteine protease with the proregion covalently linked to the active site cysteine.,Sivaraman J, Nagler DK, Zhang R, Menard R, Cygler M J Mol Biol. 2000 Jan 28;295(4):939-51. PMID:10656802<ref>PMID:10656802</ref>

-	~~===CRYSTAL STRUCTURE OF HUMAN PROCATHEPSIN X: A CYSTEINE PROTEASE WITH THE PROREGION COVALENTLY LINKED TO THE ACTIVE SITE CYSTEINE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10656802}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1deu]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA].~~	+

	==See Also==		==See Also==
	*[[Cathepsin\|Cathepsin]]		*[[Cathepsin\|Cathepsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010656802<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]

OCA at 20:55, 20 October 2012

2012-10-20T20:55:37Z

←Older revision		Revision as of 20:55, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1deu]] is a 2 chain structure ~~of [[Cathepsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA].	+	[[1deu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DEU OCA].

	==See Also==		==See Also==