1djd - Revision history

OCA at 11:44, 22 November 2023

2023-11-22T11:44:46Z

←Older revision		Revision as of 11:44, 22 November 2023
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	==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==		==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==
-	<StructureSection load='1djd' size='340' side='right'caption='[[1djd~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1djd' size='340' side='right'caption='[[1djd]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [https://pdbe.org/1djd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [https://www.ebi.ac.uk/pdbsum/1djd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1djd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [https://pdbe.org/1djd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [https://www.ebi.ac.uk/pdbsum/1djd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1djd ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harris~~, C M~~]]	+	[[Category: Harris CM]]
-	[[Category: Harris~~, T M~~]]	+	[[Category: Harris TM]]
-	[[Category: Kim~~, H Y~~]]	+	[[Category: Kim HY]]
-	[[Category: Li, Z]]	+	[[Category: Li Z]]
-	[[Category: Stone~~, M P~~]]	+	[[Category: Stone MP]]
-	[[Category: Tamura~~, P J]]~~	+	[[Category: Tamura PJ]]
-	~~[[Category: Dna~~]]	+

OCA at 18:28, 10 March 2021

2021-03-10T18:28:27Z

←Older revision		Revision as of 18:28, 10 March 2021
Line 3:		Line 3:
	<StructureSection load='1djd' size='340' side='right'caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1djd' size='340' side='right'caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DJD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [~~http~~://pdbe.org/1djd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1djd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1djd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [https://pdbe.org/1djd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [https://www.ebi.ac.uk/pdbsum/1djd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1djd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:04, 6 June 2019

2019-06-06T00:04:09Z

←Older revision		Revision as of 00:04, 6 June 2019
Line 1:		Line 1:

	==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==		==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==
-	<StructureSection load='1djd' size='340' side='right' caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1djd' size='340' side='right'caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Harris, C M]]		[[Category: Harris, C M]]
	[[Category: Harris, T M]]		[[Category: Harris, T M]]

OCA at 04:06, 30 August 2017

2017-08-30T04:06:04Z

←Older revision		Revision as of 04:06, 30 August 2017
Line 1:		Line 1:
		+
	==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==		==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==
	<StructureSection load='1djd' size='340' side='right' caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1djd' size='340' side='right' caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://pdbe.org/1djd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://pdbe.org/1djd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1djd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:58, 10 September 2015

2015-09-10T21:58:03Z

←Older revision		Revision as of 21:58, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://pdbe.org/1djd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1djd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:33, 23 December 2014

2014-12-23T00:33:23Z

←Older revision		Revision as of 00:33, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Harris, C M.]]	+	[[Category: Harris, C M]]
-	[[Category: Harris, T M.]]	+	[[Category: Harris, T M]]
-	[[Category: Kim, H Y.]]	+	[[Category: Kim, H Y]]
-	[[Category: Li, Z.]]	+	[[Category: Li, Z]]
-	[[Category: Stone, M P.]]	+	[[Category: Stone, M P]]
-	[[Category: Tamura, P J.]]	+	[[Category: Tamura, P J]]
	[[Category: Dna]]		[[Category: Dna]]

OCA at 06:34, 4 September 2014

2014-09-04T06:34:04Z

←Older revision		Revision as of 06:34, 4 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1djd.~~png~~\|~~left\|200px~~]]	+	==THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE==
		+	<StructureSection load='1djd' size='340' side='right' caption='[[1djd]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DJD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TBT:8,9,10,11-TETRAHYDRO-BENZO[A]ANTHRACENE-8,9,10-TRIOL'>TBT</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1djd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1djd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1djd RCSB], [http://www.ebi.ac.uk/pdbsum/1djd PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structure of the non-bay region (8S,9R,10S,11R)-N(6)-[11-(8,9,10, 11-tetrahydro-8,9,10-trihydroxybenz[a]anthracenyl)]-2'-de oxyadenosyl adduct at X(6) of 5'-d(CGGACXAGAAG)-3'.5'-d(CTTCTTGTCCG)-3', incorporating codons 60, 61 (underlined), and 62 of the human N-ras protooncogene, was determined. Molecular dynamics simulations were restrained by 475 NOEs from (1)H NMR. The benz[a]anthracene moiety intercalated above the 5'-face of the modified base pair and from the major groove. The duplex suffered distortion at and immediately adjacent to the adduct site. This was evidenced by the disruption of the Watson-Crick base pairing for X(6) x T(17) and A(7) x T(16) and the increased rise of 7.7 A between base pairs C(5) x G(18) and X(6) x T(17). Increased disorder was observed as excess line width of proton resonances near the lesion site. Comparison with the bay region benzo[a]pyrene [Zegar, I. S., Kim, S. J., Johansen, T. N., Horton, P. J., Harris, C. M., Harris, T. M., and Stone, M. P. (1996) Biochemistry 35, 6212-6224] and bay region benz[a]anthracene [Li, Z., Mao, H., Kim, H.-Y., Tamura, P. J., Harris, C. M., Harris, T. M., and Stone, M. P. (1999) Biochemistry 38, 2969-2981] adducts with the corresponding stereochemistry and at the same site shows that this non-bay region benz[a]anthracene lesion assumes different base pair geometry, in addition to exhibiting greater disorder. These differences are attributed to the loss of the bay region ring. The results suggest the bay region ring contributes to base stacking interactions at the lesion site. These structural differences between the non-bay and bay region lesions are correlated with site-specific mutagenesis data. The bay region benzo[a]pyrene and bay region benz[a]anthracene adducts were poorly replicated in vivo, and induced A --> G mutations. In contrast, the non-bay region benz[a]anthracene adduct was easily bypassed in vivo and was nonmutagenic.

-	~~{{STRUCTURE_1djd\| PDB=1djd \| SCENE= }}~~	+	Role of a polycyclic aromatic hydrocarbon bay region ring in modulating DNA adduct structure: the non-bay region (8S,9R,10S, 11R)-N(6)-[11-(8,9,10,11-tetrahydro-8,9, 10-trihydroxybenz[a]anthracenyl)]-2'-deoxyadenosyl adduct in codon 61 of the human N-ras protooncogene.,Li Z, Kim HY, Tamura PJ, Harris CM, Harris TM, Stone MP Biochemistry. 1999 Nov 9;38(45):14820-32. PMID:10555964<ref>PMID:10555964</ref>

-	~~===THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10555964}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1djd]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA].~~	+	</StructureSection>
	[[Category: Harris, C M.]]		[[Category: Harris, C M.]]
	[[Category: Harris, T M.]]		[[Category: Harris, T M.]]

OCA: Protected "1djd" [edit=sysop:move=sysop]

2012-10-31T09:51:53Z

Protected "1djd" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:51, 31 October 2012

OCA at 09:51, 31 October 2012

2012-10-31T09:51:50Z

←Older revision		Revision as of 09:51, 31 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1djd.png\|left\|200px]]		[[Image:1djd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1djd", creates the "Structure Box" on the page.
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	{{STRUCTURE_1djd\| PDB=1djd \| SCENE= }}		{{STRUCTURE_1djd\| PDB=1djd \| SCENE= }}

	===THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE===		===THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE===

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	{{ABSTRACT_PUBMED_10555964}}		{{ABSTRACT_PUBMED_10555964}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA].	+	[[1djd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA].
-		+
-	~~==Reference==~~	+
-	Role of a polycyclic aromatic hydrocarbon bay region ring in modulating DNA adduct structure: the non-bay region (8S,9R,10S, 11R)-N(6)-[11-(8,9,10,11-tetrahydro-8,9, 10-trihydroxybenz[a]anthracenyl)]-2'-deoxyadenosyl adduct in codon 61 of the human N-ras protooncogene., Li Z, Kim HY, Tamura PJ, Harris CM, Harris TM, Stone MP, Biochemistry. 1999 Nov 9;38(45):14820-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10555964 10555964]	+
	[[Category: Harris, C M.]]		[[Category: Harris, C M.]]
	[[Category: Harris, T M.]]		[[Category: Harris, T M.]]
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	[[Category: Stone, M P.]]		[[Category: Stone, M P.]]
	[[Category: Tamura, P J.]]		[[Category: Tamura, P J.]]
-		+	[[Category: Dna]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Jun 30 23:09:17 2008''~~	+

OCA at 20:09, 30 June 2008

2008-06-30T20:09:18Z

←Older revision		Revision as of 20:09, 30 June 2008
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-	[[Image:1djd.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1djd.png\|left\|200px]]

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	{{STRUCTURE_1djd\| PDB=1djd \| SCENE= }}		{{STRUCTURE_1djd\| PDB=1djd \| SCENE= }}

-	~~'''~~THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE~~'''~~	+	===THE SOLUTION STRUCTURE OF A NON-BAY REGION 11R-BENZ[A]ANTHRACENE OXIDE ADDUCT AT THE N6 POSITION OF ADENINE OF AN OLIGODEOXYNUCLEOTIDE CONTAINING THE HUMAN N-RAS CODON 61 SEQUENCE===


-	~~==Overview==~~	+	<!--
-	The ~~structure of the non-bay region (8S~~,9R,10S,11R)-N(6)-[11-(8,9,10, 11-tetrahydro-8,9,10-trihydroxybenz[a]anthracenyl)]-2'-de oxyadenosyl adduct at X(6) of 5'-d(CGGACXAGAAG)-3'.5'-d(CTTCTTGTCCG)-3', incorporating codons 60, 61 (underlined), and 62 of the human N-ras protooncogene, was determined. Molecular dynamics simulations were restrained by 475 NOEs from (1)H NMR. The benz[a]anthracene moiety intercalated above the 5'-face of the modified base pair and from the major groove. The duplex suffered distortion at and immediately adjacent to the ~~adduct site. This was evidenced by the disruption of the Watson-Crick base pairing for X~~(~~6) x T(17) and A(7) x T(16) and the increased rise of 7.7 A between base pairs C(5) x G(18) and X(6) x T(17). Increased disorder was observed~~ as ~~excess line width of proton resonances near the lesion site~~. ~~Comparison with the bay region benzo[a]pyrene [Zegar, I~~. ~~S., Kim, S. J., Johansen, T. N., Horton, P. J., Harris, C. M., Harris, T. M., and Stone, M. P. (1996~~) ~~Biochemistry 35~~, ~~6212-6224] and bay region benz[a]anthracene [Li, Z., Mao, H., Kim, H.-Y., Tamura, P. J., Harris, C. M., Harris, T. M., and Stone, M. P. (1999) Biochemistry 38, 2969-2981] adducts with~~ the ~~corresponding stereochemistry and at the same site shows that this non-bay region benz[a]anthracene lesion assumes different base pair geometry, in addition to exhibiting greater disorder~~. These differences are attributed to the loss of the bay region ring. The results suggest the bay region ring contributes to base stacking interactions at the lesion site. These structural differences between the non-~~bay and bay region lesions are correlated with site~~-specific mutagenesis data. The bay region benzo[a]pyrene and bay region benz[a]anthracene adducts were poorly replicated in vivo, and induced A --> G mutations. In contrast, the non-bay region benz[a]anthracene adduct was easily bypassed in vivo and was nonmutagenic.	+	The line below this paragraph, {{ABSTRACT_PUBMED_10555964}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10555964 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10555964}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DJD OCA].

	==Reference==		==Reference==
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	[[Category: Stone, M P.]]		[[Category: Stone, M P.]]
	[[Category: Tamura, P J.]]		[[Category: Tamura, P J.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 13~~:54:48 2008''	+
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