1dux - Revision history

OCA at 06:59, 7 February 2024

2024-02-07T06:59:29Z

←Older revision		Revision as of 06:59, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='1dux' size='340' side='right'caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1dux' size='340' side='right'caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [https://pdbe.org/1dux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [https://www.ebi.ac.uk/pdbsum/1dux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dux ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [https://pdbe.org/1dux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [https://www.ebi.ac.uk/pdbsum/1dux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ELK1_HUMAN ELK1_HUMAN]] Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.	+	[https://www.uniprot.org/uniprot/ELK1_HUMAN ELK1_HUMAN] Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dux ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dux ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	SAP-1 and Elk-1 are members of a large group of eukaryotic transcription factors that contain a conserved ETS DNA binding domain and that cooperate with the serum response factor (SRF) to activate transcription of the c-fos protooncogene. Despite the high degree of sequence similarity, which includes an identical amino acid sequence for the DNA recognition helix within the ETS domain of these proteins, they exhibit different DNA binding properties. Here we report the 2.1 inverted question mark crystal structure of the ETS domain of Elk-1 bound to a high affinity E74 DNA (E74DNA) site and compare it to a SAP-1-E74DNA complex. This comparison reveals that the differential DNA binding properties of these proteins are mediated by non-conserved residues distal to the DNA binding surface that function to orient conserved residues in the DNA recognition helix for protein-specific DNA contacts. As a result, nearly one-third of the interactions between the protein recognition helix and the DNA are different between the SAP-1 and Elk-1 DNA complexes. Taken together, these studies reveal a novel mechanism for the modulation of DNA binding specificity within a conserved DNA binding domain, and have implications for how highly homologous ETS proteins exhibit differential DNA-binding properties.
-
-	Structure of the elk-1-DNA complex reveals how DNA-distal residues affect ETS domain recognition of DNA.,Mo Y, Vaessen B, Johnston K, Marmorstein R Nat Struct Biol. 2000 Apr;7(4):292-7. PMID:10742173<ref>PMID:10742173</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1dux" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Johnston, K]]	+	[[Category: Johnston K]]
-	[[Category: Marmorstein, R]]	+	[[Category: Marmorstein R]]
-	[[Category: Mo, Y]]	+	[[Category: Mo Y]]
-	[[Category: Vaessen, B]]	+	[[Category: Vaessen B]]
-	~~[[Category: Dna-binding domain]]~~	+
-	~~[[Category: Dna-binding specificity]]~~	+
-	~~[[Category: Ets-domain]]~~	+
-	~~[[Category: Transcription-dna complex]]~~	+
-	~~[[Category: Winged helix-turn-helix~~]]	+

OCA at 09:31, 21 July 2021

2021-07-21T09:31:41Z

←Older revision		Revision as of 09:31, 21 July 2021
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	<StructureSection load='1dux' size='340' side='right'caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1dux' size='340' side='right'caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [~~http~~://pdbe.org/1dux PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1dux PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1dux ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [https://pdbe.org/1dux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [https://www.ebi.ac.uk/pdbsum/1dux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ELK1_HUMAN ELK1_HUMAN]] Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.	+	[[https://www.uniprot.org/uniprot/ELK1_HUMAN ELK1_HUMAN]] Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:41, 16 October 2019

2019-10-16T10:41:15Z

←Older revision		Revision as of 10:41, 16 October 2019
Line 1:		Line 1:

	==ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION==		==ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION==
-	<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1dux' size='340' side='right'caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Johnston, K]]		[[Category: Johnston, K]]
	[[Category: Marmorstein, R]]		[[Category: Marmorstein, R]]

OCA at 07:59, 13 December 2017

2017-12-13T07:59:07Z

←Older revision		Revision as of 07:59, 13 December 2017
Line 1:		Line 1:
		+
	==ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION==		==ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION==
	<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://pdbe.org/1dux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://pdbe.org/1dux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dux ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/du/1dux_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/du/1dux_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 09:22, 9 February 2016

2016-02-09T09:22:40Z

←Older revision		Revision as of 09:22, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dux ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:51, 10 September 2015

2015-09-10T20:51:11Z

←Older revision		Revision as of 20:51, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://pdbe.org/1dux PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1dux" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Johnston, K]]		[[Category: Johnston, K]]
	[[Category: Marmorstein, R]]		[[Category: Marmorstein, R]]

OCA at 05:59, 25 December 2014

2014-12-25T05:59:45Z

←Older revision		Revision as of 05:59, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ELK1_HUMAN ELK1_HUMAN]] Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:33, 22 December 2014

2014-12-22T23:33:34Z

←Older revision		Revision as of 23:33, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Johnston, K.]]	+	[[Category: Johnston, K]]
-	[[Category: Marmorstein, R.]]	+	[[Category: Marmorstein, R]]
-	[[Category: Mo, Y.]]	+	[[Category: Mo, Y]]
-	[[Category: Vaessen, B.]]	+	[[Category: Vaessen, B]]
	[[Category: Dna-binding domain]]		[[Category: Dna-binding domain]]
	[[Category: Dna-binding specificity]]		[[Category: Dna-binding specificity]]

OCA at 10:50, 10 September 2014

2014-09-10T10:50:32Z

←Older revision		Revision as of 10:50, 10 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1dux.~~png\|left~~\|200px]]	+	==ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION==
		+	<StructureSection load='1dux' size='340' side='right' caption='[[1dux]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1dux]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DUX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dux OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dux RCSB], [http://www.ebi.ac.uk/pdbsum/1dux PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/du/1dux_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SAP-1 and Elk-1 are members of a large group of eukaryotic transcription factors that contain a conserved ETS DNA binding domain and that cooperate with the serum response factor (SRF) to activate transcription of the c-fos protooncogene. Despite the high degree of sequence similarity, which includes an identical amino acid sequence for the DNA recognition helix within the ETS domain of these proteins, they exhibit different DNA binding properties. Here we report the 2.1 inverted question mark crystal structure of the ETS domain of Elk-1 bound to a high affinity E74 DNA (E74DNA) site and compare it to a SAP-1-E74DNA complex. This comparison reveals that the differential DNA binding properties of these proteins are mediated by non-conserved residues distal to the DNA binding surface that function to orient conserved residues in the DNA recognition helix for protein-specific DNA contacts. As a result, nearly one-third of the interactions between the protein recognition helix and the DNA are different between the SAP-1 and Elk-1 DNA complexes. Taken together, these studies reveal a novel mechanism for the modulation of DNA binding specificity within a conserved DNA binding domain, and have implications for how highly homologous ETS proteins exhibit differential DNA-binding properties.

-	~~{{STRUCTURE_1dux\| PDB=1dux \| SCENE= }}~~	+	Structure of the elk-1-DNA complex reveals how DNA-distal residues affect ETS domain recognition of DNA.,Mo Y, Vaessen B, Johnston K, Marmorstein R Nat Struct Biol. 2000 Apr;7(4):292-7. PMID:10742173<ref>PMID:10742173</ref>

-	~~===ELK-1~~/~~DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10742173}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1dux]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1DUX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010742173<~~/~~ref~~><~~ref group="xtra">PMID:015048824</ref><references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Johnston, K.]]		[[Category: Johnston, K.]]

OCA: Protected "1dux" [edit=sysop:move=sysop]

2012-10-31T09:57:48Z

Protected "1dux" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:57, 31 October 2012