1e46 - Revision history

OCA at 09:58, 20 March 2024

2024-03-20T09:58:20Z

←Older revision		Revision as of 09:58, 20 March 2024
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	<StructureSection load='1e46' size='340' side='right'caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1e46' size='340' side='right'caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1E46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E46 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BME:BETA~~-~~MERCAPTOETHANOL'>BME</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [https://pdbe.org/1e46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [https://www.ebi.ac.uk/pdbsum/1e46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e46 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [~~http~~://pdbe.org/1e46 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1e46 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1e46 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FUCA_ECOLI FUCA_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e46 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e46 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structures of l-fuculose-1-phosphate aldolase (FucA) with and without a ligated analogue of dihydroxyacetone phosphate (DHAP) and of a number of active center mutants have resulted in a model of the catalytic mechanism. This model has now been confirmed by structural analyses of further mutations at the zinc coordination sphere and at the phosphate site. In addition, these mutants have revealed new aspects of the catalysis: the hydroxyl group of Tyr113' (from a neighboring subunit), which sits just outside the zinc coordination sphere, steers DHAP towards a productive binding mode at the zinc ion; Glu73 contacts zinc in between the two ligand positions intended for the DHAP oxygen atoms and thus avoids blocking of these positions by a tetrahedrally coordinated hydroxy ion; the FucA polypeptide does not assume its minimum energy state but oscillates between two states of elevated energy as demonstrated by a mutant in a minimum energy state. The back and forth motion involves a mobile loop connecting the phosphate site with intersubunit motions and thus with the Brownian motion of the solvent. The phosphate group is bound strongly at a given distance to the zinc ion, which prevents the formation of too tight a DHAP:zinc complex. This observation explains our failure to find mutants that accept phosphate-free substitutes for DHAP. The FucA zinc coordination sphere is compared with that of carbonic anhydrase.
-
-	Structures of l-fuculose-1-phosphate aldolase mutants outlining motions during catalysis.,Joerger AC, Mueller-Dieckmann C, Schulz GE J Mol Biol. 2000 Nov 3;303(4):531-43. PMID:11054289<ref>PMID:11054289</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Aldolase 3D structures\|Aldolase 3D structures]]		*[[Aldolase 3D structures\|Aldolase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: L-fuculose-phosphate aldolase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Joerger~~, A C~~]]	+	[[Category: Joerger AC]]
-	[[Category: Schulz~~, G E]]~~	+	[[Category: Schulz GE]]
-	~~[[Category: Bacterial l-fucose metabolism]]~~	+
-	~~[[Category: Cleavage of l-fuculose 1-phosphate to dihydroxyacetone phosphate and l-lactaldehyde]]~~	+
-	~~[[Category: Mutant structure~~]]	+

OCA at 07:49, 4 November 2020

2020-11-04T07:49:07Z

←Older revision		Revision as of 07:49, 4 November 2020
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	==L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73S==		==L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73S==
-	<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='1e46' size='340' side='right'caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1E46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1E46 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://pdbe.org/1e46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e46 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://pdbe.org/1e46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e46 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Aldolase\|Aldolase]]	+	*[[Aldolase 3D structures\|Aldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: L-fuculose-phosphate aldolase]]		[[Category: L-fuculose-phosphate aldolase]]
		+	[[Category: Large Structures]]
	[[Category: Joerger, A C]]		[[Category: Joerger, A C]]
	[[Category: Schulz, G E]]		[[Category: Schulz, G E]]

OCA at 08:47, 30 January 2019

2019-01-30T08:47:18Z

←Older revision		Revision as of 08:47, 30 January 2019
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/1e46_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/1e46_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aldolase\|Aldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:55, 16 November 2017

2017-11-16T06:55:50Z

←Older revision		Revision as of 06:55, 16 November 2017
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-	==L-~~FUCULOSE~~ 1-~~PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT~~ E73S==	+
		+	==L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73S==
	<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://pdbe.org/1e46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://pdbe.org/1e46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e46 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Aldolase\|Aldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:38, 8 February 2016

2016-02-08T16:38:07Z

←Older revision		Revision as of 16:38, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e46 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:28, 10 September 2015

2015-09-10T13:28:40Z

←Older revision		Revision as of 13:28, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E46 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E46 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://pdbe.org/1e46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1e46" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: L-fuculose-phosphate aldolase]]		[[Category: L-fuculose-phosphate aldolase]]
	[[Category: Joerger, A C]]		[[Category: Joerger, A C]]

OCA at 22:01, 22 December 2014

2014-12-22T22:01:39Z

OCA at 10:54, 10 September 2014

2014-09-10T10:54:38Z

←Older revision		Revision as of 10:54, 10 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1e46.~~png~~\|~~left~~\|200px]]	+	==L-FUCULOSE 1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT E73S==
		+	<StructureSection load='1e46' size='340' side='right' caption='[[1e46]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E46 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e4/1e46_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of l-fuculose-1-phosphate aldolase (FucA) with and without a ligated analogue of dihydroxyacetone phosphate (DHAP) and of a number of active center mutants have resulted in a model of the catalytic mechanism. This model has now been confirmed by structural analyses of further mutations at the zinc coordination sphere and at the phosphate site. In addition, these mutants have revealed new aspects of the catalysis: the hydroxyl group of Tyr113' (from a neighboring subunit), which sits just outside the zinc coordination sphere, steers DHAP towards a productive binding mode at the zinc ion; Glu73 contacts zinc in between the two ligand positions intended for the DHAP oxygen atoms and thus avoids blocking of these positions by a tetrahedrally coordinated hydroxy ion; the FucA polypeptide does not assume its minimum energy state but oscillates between two states of elevated energy as demonstrated by a mutant in a minimum energy state. The back and forth motion involves a mobile loop connecting the phosphate site with intersubunit motions and thus with the Brownian motion of the solvent. The phosphate group is bound strongly at a given distance to the zinc ion, which prevents the formation of too tight a DHAP:zinc complex. This observation explains our failure to find mutants that accept phosphate-free substitutes for DHAP. The FucA zinc coordination sphere is compared with that of carbonic anhydrase.

-	~~{{STRUCTURE_1e46\| PDB=1e46 \| SCENE= }}~~	+	Structures of l-fuculose-1-phosphate aldolase mutants outlining motions during catalysis.,Joerger AC, Mueller-Dieckmann C, Schulz GE J Mol Biol. 2000 Nov 3;303(4):531-43. PMID:11054289<ref>PMID:11054289</ref>

-	~~===L-FUCULOSE 1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT E73S===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11054289}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1e46]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA].~~	+

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011054289<~~/~~ref~~><~~ref group="xtra">PMID:010821675</ref><ref group="xtra">PMID:008676381</ref><ref group="xtra">PMID:008515438</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: L-fuculose-phosphate aldolase]]		[[Category: L-fuculose-phosphate aldolase]]

OCA at 10:46, 20 October 2012

2012-10-20T10:46:43Z

←Older revision		Revision as of 10:46, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1e46]] is a 1 chain structure ~~of [[Aldolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA].	+	[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA].

	==See Also==		==See Also==

OCA at 03:14, 26 July 2012

2012-07-26T03:14:09Z

←Older revision		Revision as of 03:14, 26 July 2012
Line 1:		Line 1:
	[[Image:1e46.png\|left\|200px]]		[[Image:1e46.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1e46", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1e46\| PDB=1e46 \| SCENE= }}		{{STRUCTURE_1e46\| PDB=1e46 \| SCENE= }}

	===L-FUCULOSE 1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT E73S===		===L-FUCULOSE 1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI MUTANT E73S===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11054289}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11054289 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11054289}}		{{ABSTRACT_PUBMED_11054289}}

	==About this Structure==		==About this Structure==
-	[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA].	+	[[1e46]] is a 1 chain structure of [[Aldolase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA].
		+
		+	==See Also==
		+	*[[Aldolase\|Aldolase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11054289~~</ref><ref group="xtra">PMID:~~10821675~~</ref><ref group="xtra">PMID:~~8676381~~</ref><ref group="xtra">PMID:~~8515438~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011054289</ref><ref group="xtra">PMID:010821675</ref><ref group="xtra">PMID:008676381</ref><ref group="xtra">PMID:008515438</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: L-fuculose-phosphate aldolase]]		[[Category: L-fuculose-phosphate aldolase]]

←Older revision		Revision as of 22:01, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E46 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e46]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E46 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E46 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fua\|3fua]], [[2fua\|2fua]], [[1fua\|1fua]], [[1dzy\|1dzy]], [[1dzz\|1dzz]], [[1dzu\|1dzu]], [[1dzv\|1dzv]], [[1dzw\|1dzw]], [[1dzx\|1dzx]], [[4fua\|4fua]], [[1e47\|1e47]], [[1e48\|1e48]], [[1e49\|1e49]], [[1e4a\|1e4a]], [[1e4b\|1e4b]], [[1e4c\|1e4c]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e46 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e46 RCSB], [http://www.ebi.ac.uk/pdbsum/1e46 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: L-fuculose-phosphate aldolase]]		[[Category: L-fuculose-phosphate aldolase]]
-	[[Category: Joerger, A C.]]	+	[[Category: Joerger, A C]]
-	[[Category: Schulz, G E.]]	+	[[Category: Schulz, G E]]
	[[Category: Bacterial l-fucose metabolism]]		[[Category: Bacterial l-fucose metabolism]]
	[[Category: Cleavage of l-fuculose 1-phosphate to dihydroxyacetone phosphate and l-lactaldehyde]]		[[Category: Cleavage of l-fuculose 1-phosphate to dihydroxyacetone phosphate and l-lactaldehyde]]
	[[Category: Mutant structure]]		[[Category: Mutant structure]]