1e7k - Revision history

OCA at 08:47, 9 May 2024

2024-05-09T08:47:29Z

←Older revision		Revision as of 08:47, 9 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
		+
		+	Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.,Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207<ref>PMID:11163207</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1e7k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:59, 20 March 2024

2024-03-20T09:59:27Z

←Older revision		Revision as of 09:59, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [https://pdbe.org/1e7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [https://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [https://pdbe.org/1e7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [https://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN]] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>	+	[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
-
-	Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.,Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207<ref>PMID:11163207</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1e7k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ficner, R]]	+	[[Category: Ficner R]]
-	[[Category: Harthmuth, K]]	+	[[Category: Harthmuth K]]
-	[[Category: Luhrmann, R]]	+	[[Category: Luhrmann R]]
-	[[Category: Nottrott, S]]	+	[[Category: Nottrott S]]
-	[[Category: Vidovic, I]]	+	[[Category: Vidovic I]]
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna recognition motif]]~~	+
-	~~[[Category: Rna-binding protein]]~~	+
-	~~[[Category: Splicing]]~~	+
-	~~[[Category: U4 snrna~~]]	+

OCA at 11:07, 28 July 2021

2021-07-28T11:07:59Z

←Older revision		Revision as of 11:07, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1E7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [~~http~~://pdbe.org/1e7k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [https://pdbe.org/1e7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [https://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN]] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>	+	[[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN]] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	*[[Kink-turn motif\|Kink-turn motif]]		*[[Kink-turn motif\|Kink-turn motif]]
	*[[User:Wayne Decatur/kink-turn motif\|User:Wayne Decatur/kink-turn motif]]		*[[User:Wayne Decatur/kink-turn motif\|User:Wayne Decatur/kink-turn motif]]
-	*[[15.5kD/Snu13/L7Ae protein\|15.5kD/Snu13/L7Ae protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:30, 23 October 2019

2019-10-23T08:30:35Z

←Older revision		Revision as of 08:30, 23 October 2019
Line 1:		Line 1:

	==Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment==		==Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment==
-	<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='1e7k' size='340' side='right'caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Ficner, R]]		[[Category: Ficner, R]]
	[[Category: Harthmuth, K]]		[[Category: Harthmuth, K]]

OCA at 07:51, 13 December 2017

2017-12-13T07:51:44Z

←Older revision		Revision as of 07:51, 13 December 2017
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE SPLICEOSOMAL~~ 15.~~5KD PROTEIN BOUND TO A~~ U4 ~~SNRNA FRAGMENT~~==	+
		+	==Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment==
	<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://pdbe.org/1e7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://pdbe.org/1e7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 41:		Line 42:
	[[Category: Nottrott, S]]		[[Category: Nottrott, S]]
	[[Category: Vidovic, I]]		[[Category: Vidovic, I]]
		+	[[Category: Rna binding protein]]
	[[Category: Rna recognition motif]]		[[Category: Rna recognition motif]]
	[[Category: Rna-binding protein]]		[[Category: Rna-binding protein]]
	[[Category: Splicing]]		[[Category: Splicing]]
	[[Category: U4 snrna]]		[[Category: U4 snrna]]

OCA at 12:42, 9 February 2016

2016-02-09T12:42:16Z

←Older revision		Revision as of 12:42, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:21, 10 September 2015

2015-09-10T22:21:16Z

←Older revision		Revision as of 22:21, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://pdbe.org/1e7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1e7k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Ficner, R]]		[[Category: Ficner, R]]
	[[Category: Harthmuth, K]]		[[Category: Harthmuth, K]]

OCA at 06:53, 25 December 2014

2014-12-25T06:53:33Z

←Older revision		Revision as of 06:53, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN]] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:19, 22 December 2014

2014-12-22T18:19:08Z

←Older revision		Revision as of 18:19, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Ficner, R.]]	+	[[Category: Ficner, R]]
-	[[Category: Harthmuth, K.]]	+	[[Category: Harthmuth, K]]
-	[[Category: Luhrmann, R.]]	+	[[Category: Luhrmann, R]]
-	[[Category: Nottrott, S.]]	+	[[Category: Nottrott, S]]
-	[[Category: Vidovic, I.]]	+	[[Category: Vidovic, I]]
	[[Category: Rna recognition motif]]		[[Category: Rna recognition motif]]
	[[Category: Rna-binding protein]]		[[Category: Rna-binding protein]]
	[[Category: Splicing]]		[[Category: Splicing]]
	[[Category: U4 snrna]]		[[Category: U4 snrna]]

OCA at 13:53, 29 September 2014

2014-09-29T13:53:46Z

←Older revision		Revision as of 13:53, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1e7k\| PDB=1e7k \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT==
-	===CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT===	+	<StructureSection load='1e7k' size='340' side='right' caption='[[1e7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_11163207}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1e7k]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.

-	~~==About this Structure==~~	+	Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.,Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207<ref>PMID:11163207</ref>
-	~~[[1e7k]] is a 4 chain~~ structure ~~with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1E7K OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Kink-turn motif\|Kink-turn motif]]		*[[Kink-turn motif\|Kink-turn motif]]
	*[[User:Wayne Decatur/kink-turn motif\|User:Wayne Decatur/kink-turn motif]]		*[[User:Wayne Decatur/kink-turn motif\|User:Wayne Decatur/kink-turn motif]]
-	*[[15.5kD/Snu13/L7Ae\|15.5kD/Snu13/L7Ae]]	+	*[[15.5kD/Snu13/L7Ae protein\|15.5kD/Snu13/L7Ae protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011163207<~~/~~ref~~><~~ref group="xtra">PMID:010545122</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ficner, R.]]		[[Category: Ficner, R.]]