1e7s - Revision history

OCA at 11:55, 13 December 2023

2023-12-13T11:55:13Z

←Older revision		Revision as of 11:55, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1e7s' size='340' side='right'caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1e7s' size='340' side='right'caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [https://pdbe.org/1e7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [https://www.ebi.ac.uk/pdbsum/1e7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [https://pdbe.org/1e7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [https://www.ebi.ac.uk/pdbsum/1e7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI]] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref>	+	[https://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Izzo, G]]	+	[[Category: Izzo G]]
-	[[Category: Rosano, C]]	+	[[Category: Rosano C]]
-	[[Category: Zuccotti, S]]	+	[[Category: Zuccotti S]]
-	~~[[Category: Epimerase-reductase complex]]~~	+
-	~~[[Category: Epimerase/reductase]]~~	+
-	~~[[Category: Red]]~~	+
-	~~[[Category: Sdr~~]]	+

OCA at 10:23, 17 February 2021

2021-02-17T10:23:57Z

←Older revision		Revision as of 10:23, 17 February 2021
Line 3:		Line 3:
	<StructureSection load='1e7s' size='340' side='right'caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1e7s' size='340' side='right'caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1E7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [~~http~~://pdbe.org/1e7s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1e7s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [https://pdbe.org/1e7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [https://www.ebi.ac.uk/pdbsum/1e7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI]] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref>	+	[[https://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI]] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:32, 10 May 2019

2019-05-10T11:32:32Z

←Older revision		Revision as of 11:32, 10 May 2019
Line 1:		Line 1:

	==GDP 4-keto-6-deoxy-D-mannose epimerase reductase K140R==		==GDP 4-keto-6-deoxy-D-mannose epimerase reductase K140R==
-	<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='1e7s' size='340' side='right'caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Izzo, G]]		[[Category: Izzo, G]]

OCA at 08:05, 13 December 2017

2017-12-13T08:05:44Z

←Older revision		Revision as of 08:05, 13 December 2017
Line 1:		Line 1:
-	==GDP 4-~~KETO~~-6-~~DEOXY~~-D-~~MANNOSE EPIMERASE REDUCTASE~~ K140R==	+
		+	==GDP 4-keto-6-deoxy-D-mannose epimerase reductase K140R==
	<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://pdbe.org/1e7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://pdbe.org/1e7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e7s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7s_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7s_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:35, 9 February 2016

2016-02-09T11:35:14Z

←Older revision		Revision as of 11:35, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e7s ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:48, 10 September 2015

2015-09-10T21:48:39Z

←Older revision		Revision as of 21:48, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://pdbe.org/1e7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1e7s" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Izzo, G]]		[[Category: Izzo, G]]

OCA at 06:32, 25 December 2014

2014-12-25T06:32:20Z

←Older revision		Revision as of 06:32, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI]] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 00:26, 23 December 2014

2014-12-23T00:26:08Z

←Older revision		Revision as of 00:26, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Bolognesi, M.]]	+	[[Category: Bolognesi, M]]
-	[[Category: Izzo, G.]]	+	[[Category: Izzo, G]]
-	[[Category: Rosano, C.]]	+	[[Category: Rosano, C]]
-	[[Category: Zuccotti, S.]]	+	[[Category: Zuccotti, S]]
	[[Category: Epimerase-reductase complex]]		[[Category: Epimerase-reductase complex]]
	[[Category: Epimerase/reductase]]		[[Category: Epimerase/reductase]]
	[[Category: Red]]		[[Category: Red]]
	[[Category: Sdr]]		[[Category: Sdr]]

OCA at 10:58, 10 September 2014

2014-09-10T10:58:01Z

←Older revision		Revision as of 10:58, 10 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1e7s.~~png~~\|~~left~~\|200px]]	+	==GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE K140R==
		+	<StructureSection load='1e7s' size='340' side='right' caption='[[1e7s]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1e7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E7S FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bws\|1bws]], [[1bsv\|1bsv]], [[1e6u\|1e6u]], [[1e7q\|1e7q]], [[1e7r\|1e7r]], [[1fxs\|1fxs]], [[1gfs\|1gfs]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e7s RCSB], [http://www.ebi.ac.uk/pdbsum/1e7s PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/1e7s_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GDP-4-keto-6-deoxy-d-mannose epimerase/reductase is a bifunctional enzyme responsible for the last step in the biosynthesis of GDP-l-fucose, the substrate of fucosyl transferases. Several cell-surface antigens, including the leukocyte Lewis system and cell-surface antigens in pathogenic bacteria, depend on the availability of GDP-l-fucose for their expression. Therefore, the enzyme is a potential target for therapy in pathological states depending on selectin-mediated cell-to-cell interactions. Previous crystallographic investigations have shown that GDP-4-keto-6-deoxy-d-mannose epimerase/reductase belongs to the short-chain dehydrogenase/reductase protein homology family. The enzyme active-site region is at the interface of an N-terminal NADPH-binding domain and a C-terminal domain, held to bind the substrate. The design, expression and functional characterization of seven site-specific mutant forms of GDP-4-keto-6-deoxy-d-mannose epimerase/reductase are reported here. In parallel, the crystal structures of the native holoenzyme and of three mutants (Ser107Ala, Tyr136Glu and Lys140Arg) have been investigated and refined at 1. 45-1.60 A resolution, based on synchrotron data (R-factors range between 12.6 % and 13.9 %). The refined protein models show that besides the active-site residues Ser107, Tyr136 and Lys140, whose mutations impair the overall enzymatic activity and may affect the coenzyme binding mode, side-chains capable of proton exchange, located around the expected substrate (GDP-4-keto-6-deoxy-d-mannose) binding pocket, are selectively required during the epimerization and reduction steps. Among these, Cys109 and His179 may play a primary role in proton exchange between the enzyme and the epimerization catalytic intermediates. Finally, the additional role of mutated active-site residues involved in substrate recognition and in enzyme stability has been analyzed.

-	~~{{STRUCTURE_1e7s\| PDB=1e7s \| SCENE= }}~~	+	Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose Epimerase/Reductase by kinetic and crystallographic characterization of site-specific mutants.,Rosano C, Bisso A, Izzo G, Tonetti M, Sturla L, De Flora A, Bolognesi M J Mol Biol. 2000 Oct 13;303(1):77-91. PMID:11021971<ref>PMID:11021971</ref>

-	~~===GDP 4-KETO-6-DEOXY-D-MANNOSE EPIMERASE REDUCTASE K140R===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~==About this Structure==~~	+	<references/>
-	~~[[1e7s]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1E7S OCA].~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011021971<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Bolognesi, M.]]		[[Category: Bolognesi, M.]]

OCA: Protected "1e7s" [edit=sysop:move=sysop]

2012-11-08T14:44:42Z

Protected "1e7s" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:44, 8 November 2012