1eit - Revision history

OCA at 11:47, 22 November 2023

2023-11-22T11:47:55Z

←Older revision		Revision as of 11:47, 22 November 2023
Line 1:		Line 1:

	==NMR STUDY OF MU-AGATOXIN==		==NMR STUDY OF MU-AGATOXIN==
-	<StructureSection load='1eit' size='340' side='right'caption='[[1eit~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1eit' size='340' side='right'caption='[[1eit]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [https://pdbe.org/1eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [https://www.ebi.ac.uk/pdbsum/1eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [https://pdbe.org/1eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [https://www.ebi.ac.uk/pdbsum/1eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TXMG1_AGEAP TXMG1_AGEAP]~~] Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons.<ref>PMID:2914898</ref>	+	[https://www.uniprot.org/uniprot/T5G1A_AGEAP T5G1A_AGEAP] Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons.<ref>PMID:2914898</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 25:
	[[Category: Agelenopsis aperta]]		[[Category: Agelenopsis aperta]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Omecinsky~~, D O~~]]	+	[[Category: Omecinsky DO]]
-	[[Category: Reily~~, M D]]~~	+	[[Category: Reily MD]]
-	~~[[Category: Excitatory insect toxin]]~~	+
-	~~[[Category: Neurotoxin~~]]	+

OCA at 07:11, 17 March 2021

2021-03-17T07:11:54Z

←Older revision		Revision as of 07:11, 17 March 2021
Line 3:		Line 3:
	<StructureSection load='1eit' size='340' side='right'caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1eit' size='340' side='right'caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [~~http~~://pdbe.org/1eit PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1eit PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1eit ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [https://pdbe.org/1eit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [https://www.ebi.ac.uk/pdbsum/1eit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TXMG1_AGEAP TXMG1_AGEAP]] Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons.<ref>PMID:2914898</ref>	+	[[https://www.uniprot.org/uniprot/TXMG1_AGEAP TXMG1_AGEAP]] Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons.<ref>PMID:2914898</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:30, 12 June 2019

2019-06-12T06:30:40Z

←Older revision		Revision as of 06:30, 12 June 2019
Line 1:		Line 1:

	==NMR STUDY OF MU-AGATOXIN==		==NMR STUDY OF MU-AGATOXIN==
-	<StructureSection load='1eit' size='340' side='right' caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1eit' size='340' side='right'caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Agelenopsis aperta]]		[[Category: Agelenopsis aperta]]
		+	[[Category: Large Structures]]
	[[Category: Omecinsky, D O]]		[[Category: Omecinsky, D O]]
	[[Category: Reily, M D]]		[[Category: Reily, M D]]
	[[Category: Excitatory insect toxin]]		[[Category: Excitatory insect toxin]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]

OCA at 03:45, 6 September 2017

2017-09-06T03:45:51Z

←Older revision		Revision as of 03:45, 6 September 2017
Line 1:		Line 1:
		+
	==NMR STUDY OF MU-AGATOXIN==		==NMR STUDY OF MU-AGATOXIN==
	<StructureSection load='1eit' size='340' side='right' caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1eit' size='340' side='right' caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://pdbe.org/1eit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://pdbe.org/1eit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 06:37, 11 September 2015

2015-09-11T06:37:23Z

←Older revision		Revision as of 06:37, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://pdbe.org/1eit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1eit" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:41, 25 December 2014

2014-12-25T12:41:01Z

←Older revision		Revision as of 12:41, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TXMG1_AGEAP TXMG1_AGEAP]] Insecticidal neurotoxin that induces an irreversible spastic paralysis when injected into insects. Modifies presynaptic voltage-gated sodium channels (Nav), causing them to open at the normal resting potential of the nerve. This leads to spontaneous release of neurotransmitter and repetitive action potentials in motor neurons.<ref>PMID:2914898</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 20:54, 22 December 2014

2014-12-22T20:54:55Z

←Older revision		Revision as of 20:54, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Agelenopsis aperta]]		[[Category: Agelenopsis aperta]]
-	[[Category: Omecinsky, D O.]]	+	[[Category: Omecinsky, D O]]
-	[[Category: Reily, M D.]]	+	[[Category: Reily, M D]]
	[[Category: Excitatory insect toxin]]		[[Category: Excitatory insect toxin]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]

OCA at 11:12, 24 September 2014

2014-09-24T11:12:45Z

←Older revision		Revision as of 11:12, 24 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1eit.~~png~~\|~~left\|200px~~]]	+	==NMR STUDY OF MU-AGATOXIN==
		+	<StructureSection load='1eit' size='340' side='right' caption='[[1eit]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EIT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eit RCSB], [http://www.ebi.ac.uk/pdbsum/1eit PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report the solution structure of mu-agatoxin-I (mu-Aga-I) and model structures of the closely related mu-agatoxin-IV (mu-Aga-IV) which were isolated from venom of the American funnel web spider, Agelenopsis aperta. These toxins, which modify the kinetics of neuronal voltage-activated sodium channels in insects, are C-terminally amidated peptides composed to 36 amino acids, including four internal disulfide bonds. The structure of mu-Aga-I was determined by NMR and distance geometry/molecular dynamics calculations. Structural calculations were carried out using 256 interresidue NOE-derived distance restraints and 25 angle restraints obtained from vicinal coupling constants. The peptide contains eight cysteines involved in disulfide bonds, the pairings of which were uncertain and had to be determined from preliminary structure calculations. The toxin has an average rmsd of 0.89 A for the backbone atoms among 38 converged conformers. The structure consists of a well-defined triple-stranded beta-sheet involving residues 7-9, 20-24, and 30-34 and four tight turns. A homologous peptide, mu-Aga-IV, exhibited two distinct and equally populated conformations in solution, which complicated spectral analysis. Analysis of sequential NOE's confirmed that the conformers arose from cis and trans peptide bonds involving a proline at position 15. Models were developed for both conformers based on the mu-Aga-I structure. Our structural data show that the mu-agatoxins, although specific modifiers of sodium channels, share common secondary and tertiary structural motifs with phylogenetically diverse peptide toxins targeting a variety of channel types. The mu-agatoxins add voltage-sensitive sodium channel activity to a growing list of neurotoxic effects elicited by peptide toxins which share the same global fold yet differ in their animal origin and ion channel selectivity.

-	~~{{STRUCTURE_1eit\| PDB=1eit \| SCENE= }}~~	+	Three-dimensional structure analysis of mu-agatoxins: further evidence for common motifs among neurotoxins with diverse ion channel specificities.,Omecinsky DO, Holub KE, Adams ME, Reily MD Biochemistry. 1996 Mar 5;35(9):2836-44. PMID:8608119<ref>PMID:8608119</ref>

-	~~===NMR STUDY OF MU-AGATOXIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~==About this Structure==~~	+	<references/>
-	~~[[1eit]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Agelenopsis_aperta Agelenopsis aperta]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA].~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008608119<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Agelenopsis aperta]]		[[Category: Agelenopsis aperta]]
	[[Category: Omecinsky, D O.]]		[[Category: Omecinsky, D O.]]

OCA: Protected "1eit" [edit=sysop:move=sysop]

2012-11-08T14:20:55Z

Protected "1eit" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:20, 8 November 2012

OCA at 14:20, 8 November 2012

2012-11-08T14:20:53Z

←Older revision		Revision as of 14:20, 8 November 2012
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-	{{Seed}}
	[[Image:1eit.png\|left\|200px]]		[[Image:1eit.png\|left\|200px]]

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	{{STRUCTURE_1eit\| PDB=1eit \| SCENE= }}		{{STRUCTURE_1eit\| PDB=1eit \| SCENE= }}

	===NMR STUDY OF MU-AGATOXIN===		===NMR STUDY OF MU-AGATOXIN===

-
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-	{{ABSTRACT_PUBMED_8608119}}

	==About this Structure==		==About this Structure==
-	~~1EIT is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA].	+	[[1eit]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agelenopsis_aperta Agelenopsis aperta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIT OCA].

	==Reference==		==Reference==
-	Three-dimensional structure analysis of mu-agatoxins: further evidence for common motifs among neurotoxins with diverse ion channel specificities., Omecinsky DO, Holub KE, Adams ME, Reily MD, Biochemistry. 1996 Mar 5;35(9):2836-44. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~8608119 8608119]~~	+	<ref group="xtra">PMID:008608119</ref><references group="xtra"/>
	[[Category: Agelenopsis aperta]]		[[Category: Agelenopsis aperta]]
-	[[Category: Single protein]]
	[[Category: Omecinsky, D O.]]		[[Category: Omecinsky, D O.]]
	[[Category: Reily, M D.]]		[[Category: Reily, M D.]]
	[[Category: Excitatory insect toxin]]		[[Category: Excitatory insect toxin]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:48:40 2008''