1eoi - Revision history

OCA at 10:04, 20 March 2024

2024-03-20T10:04:32Z

←Older revision		Revision as of 10:04, 20 March 2024
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	<StructureSection load='1eoi' size='340' side='right'caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1eoi' size='340' side='right'caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_29907 Atcc 29907~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Shimwellia_blattae Shimwellia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1d2t\|1d2t]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [https://pdbe.org/1eoi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [https://www.ebi.ac.uk/pdbsum/1eoi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [https://pdbe.org/1eoi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [https://www.ebi.ac.uk/pdbsum/1eoi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9S1A6_SHIBL Q9S1A6_SHIBL]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eoi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eoi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The structure of Escherichia blattae non-specific acid phosphatase (EB-NSAP) has been determined at 1.9 A resolution with a bound sulfate marking the phosphate-binding site. The enzyme is a 150 kDa homohexamer. EB-NSAP shares a conserved sequence motif not only with several lipid phosphatases and the mammalian glucose-6-phosphatases, but also with the vanadium-containing chloroperoxidase (CPO) of Curvularia inaequalis. Comparison of the crystal structures of EB-NSAP and CPO reveals striking similarity in the active site structures. In addition, the topology of the EB-NSAP core shows considerable similarity to the fold of the active site containing part of the monomeric 67 kDa CPO, despite the lack of further sequence identity. These two enzymes are apparently related by divergent evolution. We have also determined the crystal structure of EB-NSAP complexed with the transition-state analog molybdate. Structural comparison of the native enzyme and the enzyme-molybdate complex reveals that the side-chain of His150, a putative catalytic residue, moves toward the molybdate so that it forms a hydrogen bond with the metal oxyanion when the molybdenum forms a covalent bond with NE2 of His189.
-
-	X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate.,Ishikawa K, Mihara Y, Gondoh K, Suzuki E, Asano Y EMBO J. 2000 Jun 1;19(11):2412-23. PMID:10835340<ref>PMID:10835340</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]		*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Acid phosphatase]]
-	[[Category: Atcc 29907]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Asano, Y]]	+	[[Category: Shimwellia blattae]]
-	[[Category: Gondoh, K]]	+	[[Category: Asano Y]]
-	[[Category: Ishikawa, K]]	+	[[Category: Gondoh K]]
-	[[Category: Mihara, Y]]	+	[[Category: Ishikawa K]]
-	[[Category: Suzuki, E]]	+	[[Category: Mihara Y]]
-	~~[[Category: All-alpha]]~~	+	[[Category: Suzuki E]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:39, 21 July 2021

2021-07-21T09:39:54Z

←Older revision		Revision as of 09:39, 21 July 2021
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	<StructureSection load='1eoi' size='340' side='right'caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1eoi' size='340' side='right'caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_29907 Atcc 29907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_29907 Atcc 29907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d2t\|1d2t]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [~~http~~://pdbe.org/1eoi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1eoi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [https://pdbe.org/1eoi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [https://www.ebi.ac.uk/pdbsum/1eoi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 13:12, 16 October 2019

2019-10-16T13:12:00Z

←Older revision		Revision as of 13:12, 16 October 2019
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	==CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE==		==CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE==
-	<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='1eoi' size='340' side='right'caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29907 Atcc 29907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29907 Atcc 29907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Atcc 29907]]		[[Category: Atcc 29907]]
		+	[[Category: Large Structures]]
	[[Category: Asano, Y]]		[[Category: Asano, Y]]
	[[Category: Gondoh, K]]		[[Category: Gondoh, K]]

OCA at 07:07, 20 December 2017

2017-12-20T07:07:17Z

←Older revision		Revision as of 07:07, 20 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE==		==CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE==
	<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://pdbe.org/1eoi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://pdbe.org/1eoi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eoi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eoi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eoi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Acid phosphatase\|Acid phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:09, 8 February 2016

2016-02-08T15:09:01Z

←Older revision		Revision as of 15:09, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eoi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:47, 10 September 2015

2015-09-10T12:47:50Z

←Older revision		Revision as of 12:47, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_blattae Escherichia blattae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29907 Atcc 29907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://pdbe.org/1eoi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1eoi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
-	[[Category: ~~Escherichia blattae~~]]	+	[[Category: Atcc 29907]]
	[[Category: Asano, Y]]		[[Category: Asano, Y]]
	[[Category: Gondoh, K]]		[[Category: Gondoh, K]]

OCA at 21:25, 22 December 2014

2014-12-22T21:25:28Z

←Older revision		Revision as of 21:25, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_blattae Escherichia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_blattae Escherichia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Escherichia blattae]]		[[Category: Escherichia blattae]]
-	[[Category: Asano, Y.]]	+	[[Category: Asano, Y]]
-	[[Category: Gondoh, K.]]	+	[[Category: Gondoh, K]]
-	[[Category: Ishikawa, K.]]	+	[[Category: Ishikawa, K]]
-	[[Category: Mihara, Y.]]	+	[[Category: Mihara, Y]]
-	[[Category: Suzuki, E.]]	+	[[Category: Suzuki, E]]
	[[Category: All-alpha]]		[[Category: All-alpha]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 11:17, 24 September 2014

2014-09-24T11:17:14Z

←Older revision		Revision as of 11:17, 24 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1eoi.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE==
		+	<StructureSection load='1eoi' size='340' side='right' caption='[[1eoi]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_blattae Escherichia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EOI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d2t\|1d2t]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eoi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eoi RCSB], [http://www.ebi.ac.uk/pdbsum/1eoi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/1eoi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structure of Escherichia blattae non-specific acid phosphatase (EB-NSAP) has been determined at 1.9 A resolution with a bound sulfate marking the phosphate-binding site. The enzyme is a 150 kDa homohexamer. EB-NSAP shares a conserved sequence motif not only with several lipid phosphatases and the mammalian glucose-6-phosphatases, but also with the vanadium-containing chloroperoxidase (CPO) of Curvularia inaequalis. Comparison of the crystal structures of EB-NSAP and CPO reveals striking similarity in the active site structures. In addition, the topology of the EB-NSAP core shows considerable similarity to the fold of the active site containing part of the monomeric 67 kDa CPO, despite the lack of further sequence identity. These two enzymes are apparently related by divergent evolution. We have also determined the crystal structure of EB-NSAP complexed with the transition-state analog molybdate. Structural comparison of the native enzyme and the enzyme-molybdate complex reveals that the side-chain of His150, a putative catalytic residue, moves toward the molybdate so that it forms a hydrogen bond with the metal oxyanion when the molybdenum forms a covalent bond with NE2 of His189.

-	~~{{STRUCTURE_1eoi\| PDB=1eoi \| SCENE= }}~~	+	X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate.,Ishikawa K, Mihara Y, Gondoh K, Suzuki E, Asano Y EMBO J. 2000 Jun 1;19(11):2412-23. PMID:10835340<ref>PMID:10835340</ref>

-	~~===CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE COMPLEXED WITH THE TRANSITION STATE ANALOG MOLYBDATE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10835340}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1eoi]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_blattae Escherichia blattae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA].~~	+

	==See Also==		==See Also==
	*[[Acid phosphatase\|Acid phosphatase]]		*[[Acid phosphatase\|Acid phosphatase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010835340<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Acid phosphatase]]		[[Category: Acid phosphatase]]
	[[Category: Escherichia blattae]]		[[Category: Escherichia blattae]]

OCA at 10:33, 20 October 2012

2012-10-20T10:33:53Z

←Older revision		Revision as of 10:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1eoi]] is a 3 chain structure ~~of [[Acid phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_blattae Escherichia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA].	+	[[1eoi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_blattae Escherichia blattae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EOI OCA].

	==See Also==		==See Also==

OCA: Protected "1eoi" [edit=sysop:move=sysop]

2012-07-26T02:31:41Z

Protected "1eoi" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:31, 26 July 2012