1eu8 - Revision history

OCA at 07:07, 7 February 2024

2024-02-07T07:07:04Z

←Older revision		Revision as of 07:07, 7 February 2024
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	<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'caldococcus_litoralis'_z-1301 'caldococcus~~ litoralis~~' z-1301~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PT:~~PLATINUM+(II)+ION~~'>PT</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[1omp\|1omp]], [[3mbp\|3mbp]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [https://pdbe.org/1eu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [https://pdbe.org/1eu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_THELN MALE_THELN] Part of the ABC transporter complex MalEFGK involved in trehalose/maltose import. Binds maltose and trehalose.<ref>PMID:11453995</ref> <ref>PMID:16532450</ref> <ref>PMID:8759837</ref> <ref>PMID:9457875</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eu8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eu8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We report the crystallization and structure determination at 1.85 A of the extracellular, membrane-anchored trehalose/maltose-binding protein (TMBP) in complex with its substrate trehalose. TMBP is the substrate recognition site of the high-affinity trehalose/maltose ABC transporter of the hyperthermophilic Archaeon Thermococcus litoralis. In vivo, this protein is anchored to the membrane, presumably via an N-terminal cysteine lipid modification. The crystallized protein was N-terminally truncated, resulting in a soluble protein exhibiting the same binding characteristics as the wild-type protein. The protein shows the characteristic features of a transport-related, substrate-binding protein and is structurally related to the maltose-binding protein (MBP) of Escherichia coli. It consists of two similar lobes, each formed by a parallel beta-sheet flanked by alpha-helices on both sides. Both are connected by a hinge region consisting of two antiparallel beta-strands and an alpha-helix. As in MBP, the substrate is bound in the cleft between the lobes by hydrogen bonds and hydrophobic interactions. However, compared to maltose binding in MBP, direct hydrogen bonding between the substrate and the protein prevails while apolar contacts are reduced. To elucidate factors contributing to thermostability, we compared TMBP with its mesophilic counterpart MBP and found differences known from similar investigations. Specifically, we find helices that are longer than their structurally equivalent counterparts, and fewer internal cavities.
-
-	The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A.,Diez J, Diederichs K, Greller G, Horlacher R, Boos W, Welte W J Mol Biol. 2001 Jan 26;305(4):905-15. PMID:11162101<ref>PMID:11162101</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1eu8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Caldococcus litoralis z-1301]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boos, W]]	+	[[Category: Thermococcus litoralis]]
-	[[Category: Diederichs, K]]	+	[[Category: Boos W]]
-	[[Category: Diez, J]]	+	[[Category: Diederichs K]]
-	[[Category: Greller, G]]	+	[[Category: Diez J]]
-	[[Category: Horlacher, R]]	+	[[Category: Greller G]]
-	[[Category: Welte, W]]	+	[[Category: Horlacher R]]
-	~~[[Category: Abc transporter fold]]~~	+	[[Category: Welte W]]
-	~~[[Category: Mbp 2 fold]]~~	+
-	~~[[Category: Protein-carbohydrate complex]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Thermophilic protein]]~~	+
-	~~[[Category: Trehalose-maltose binding protein~~]]	+

OCA at 09:41, 21 July 2021

2021-07-21T09:41:55Z

←Older revision		Revision as of 09:41, 21 July 2021
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	<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:~~PLATINUM+(II)+ION~~'>PT</scene>, <scene name='pdbligand=~~TRE~~:~~TREHALOSE~~'>~~TRE~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1omp\|1omp]], [[3mbp\|3mbp]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [~~http~~://pdbe.org/1eu8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [https://pdbe.org/1eu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:51, 16 October 2019

2019-10-16T10:51:55Z

←Older revision		Revision as of 10:51, 16 October 2019
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	==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==		==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==
-	<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1eu8' size='340' side='right'caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Caldococcus litoralis z-1301]]		[[Category: Caldococcus litoralis z-1301]]
		+	[[Category: Large Structures]]
	[[Category: Boos, W]]		[[Category: Boos, W]]
	[[Category: Diederichs, K]]		[[Category: Diederichs, K]]

OCA at 07:16, 20 December 2017

2017-12-20T07:16:45Z

←Older revision		Revision as of 07:16, 20 December 2017
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		+
	==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==		==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==
	<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://pdbe.org/1eu8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://pdbe.org/1eu8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eu8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1eu8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1eu8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:46, 7 February 2016

2016-02-07T08:46:47Z

←Older revision		Revision as of 08:46, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eu8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:46, 9 September 2015

2015-09-09T23:46:05Z

←Older revision		Revision as of 23:46, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermococcus_litoralis Thermococcus~~ litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'caldococcus_litoralis'_z-1301 'caldococcus litoralis' z-1301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://pdbe.org/1eu8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1eu8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermococcus~~ litoralis]]	+	[[Category: Caldococcus litoralis z-1301]]
	[[Category: Boos, W]]		[[Category: Boos, W]]
	[[Category: Diederichs, K]]		[[Category: Diederichs, K]]

OCA at 21:10, 22 December 2014

2014-12-22T21:10:16Z

←Older revision		Revision as of 21:10, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Thermococcus litoralis]]		[[Category: Thermococcus litoralis]]
-	[[Category: Boos, W.]]	+	[[Category: Boos, W]]
-	[[Category: Diederichs, K.]]	+	[[Category: Diederichs, K]]
-	[[Category: Diez, J.]]	+	[[Category: Diez, J]]
-	[[Category: Greller, G.]]	+	[[Category: Greller, G]]
-	[[Category: Horlacher, R.]]	+	[[Category: Horlacher, R]]
-	[[Category: Welte, W.]]	+	[[Category: Welte, W]]
	[[Category: Abc transporter fold]]		[[Category: Abc transporter fold]]
	[[Category: Mbp 2 fold]]		[[Category: Mbp 2 fold]]

OCA at 11:26, 24 September 2014

2014-09-24T11:26:19Z

←Older revision		Revision as of 11:26, 24 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1eu8.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS==
		+	<StructureSection load='1eu8' size='340' side='right' caption='[[1eu8]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EU8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1omp\|1omp]], [[3mbp\|3mbp]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eu8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eu8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eu8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1eu8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report the crystallization and structure determination at 1.85 A of the extracellular, membrane-anchored trehalose/maltose-binding protein (TMBP) in complex with its substrate trehalose. TMBP is the substrate recognition site of the high-affinity trehalose/maltose ABC transporter of the hyperthermophilic Archaeon Thermococcus litoralis. In vivo, this protein is anchored to the membrane, presumably via an N-terminal cysteine lipid modification. The crystallized protein was N-terminally truncated, resulting in a soluble protein exhibiting the same binding characteristics as the wild-type protein. The protein shows the characteristic features of a transport-related, substrate-binding protein and is structurally related to the maltose-binding protein (MBP) of Escherichia coli. It consists of two similar lobes, each formed by a parallel beta-sheet flanked by alpha-helices on both sides. Both are connected by a hinge region consisting of two antiparallel beta-strands and an alpha-helix. As in MBP, the substrate is bound in the cleft between the lobes by hydrogen bonds and hydrophobic interactions. However, compared to maltose binding in MBP, direct hydrogen bonding between the substrate and the protein prevails while apolar contacts are reduced. To elucidate factors contributing to thermostability, we compared TMBP with its mesophilic counterpart MBP and found differences known from similar investigations. Specifically, we find helices that are longer than their structurally equivalent counterparts, and fewer internal cavities.

-	~~{{STRUCTURE_1eu8\| PDB=1eu8 \| SCENE= }}~~	+	The crystal structure of a liganded trehalose/maltose-binding protein from the hyperthermophilic Archaeon Thermococcus litoralis at 1.85 A.,Diez J, Diederichs K, Greller G, Horlacher R, Boos W, Welte W J Mol Biol. 2001 Jan 26;305(4):905-15. PMID:11162101<ref>PMID:11162101</ref>

-	~~===STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~==About this Structure==~~	+	<references/>
-	~~[[1eu8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermococcus_litoralis Thermococcus litoralis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA].~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011162101<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermococcus litoralis]]		[[Category: Thermococcus litoralis]]
	[[Category: Boos, W.]]		[[Category: Boos, W.]]

OCA: Protected "1eu8" [edit=sysop:move=sysop]

2012-11-14T07:39:41Z

Protected "1eu8" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:39, 14 November 2012

OCA at 07:39, 14 November 2012

2012-11-14T07:39:38Z

←Older revision		Revision as of 07:39, 14 November 2012
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-	{{Seed}}
	[[Image:1eu8.png\|left\|200px]]		[[Image:1eu8.png\|left\|200px]]

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	{{STRUCTURE_1eu8\| PDB=1eu8 \| SCENE= }}		{{STRUCTURE_1eu8\| PDB=1eu8 \| SCENE= }}

	===STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS===		===STRUCTURE OF TREHALOSE MALTOSE BINDING PROTEIN FROM THERMOCOCCUS LITORALIS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11162101}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11162101 is the PubMed ID number.
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-	{{ABSTRACT_PUBMED_11162101}}

	==About this Structure==		==About this Structure==
-	~~1EU8~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA].	+	[[1eu8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_litoralis Thermococcus litoralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EU8 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11162101~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011162101</ref><references group="xtra"/>
	[[Category: Thermococcus litoralis]]		[[Category: Thermococcus litoralis]]
	[[Category: Boos, W.]]		[[Category: Boos, W.]]
Line 34:		Line 21:
	[[Category: Mbp 2 fold]]		[[Category: Mbp 2 fold]]
	[[Category: Protein-carbohydrate complex]]		[[Category: Protein-carbohydrate complex]]
		+	[[Category: Sugar binding protein]]
	[[Category: Thermophilic protein]]		[[Category: Thermophilic protein]]
	[[Category: Trehalose-maltose binding protein]]		[[Category: Trehalose-maltose binding protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:40:41 2009''