1ew0 - Revision history

OCA at 07:07, 7 February 2024

2024-02-07T07:07:43Z

←Older revision		Revision as of 07:07, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='1ew0' size='340' side='right'caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1ew0' size='340' side='right'caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_9930 Atcc 9930~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1d06\|1d06]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [https://pdbe.org/1ew0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ew0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [https://pdbe.org/1ew0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ew0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FIXL_RHIME FIXL_RHIME]] Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.	+	[https://www.uniprot.org/uniprot/FIXL_RHIME FIXL_RHIME] Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ew0 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ew0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	FixL of Rhizobium meliloti (RmFixL) is a sensor histidine kinase of the two-component system, which regulates the expression of the genes related to nitrogen fixation in the root nodule in response to the O(2) levels. The crystal structure of the sensor domain of FixL (RmFixLH), which contains a heme (Fe-porphyrin) as a sensing site, was determined at 1.4 A resolution. Based on the structural and spectroscopic analyses, we propose the O(2) sensing mechanism that differs from the case proposed in BjFixLH as follows; conformational changes in the F/G loop, which are induced by steric repulsion between the bent-bound O(2) and the Ile209 side-chain, would be transmitted to the histidine kinase domain. Interaction between the iron-bound O(2) and Ile209 was also observed in the resonance Raman spectra of RmFixLH as evidenced by the fact that the Fe-O(2) and Fe-CN stretching frequencies were shifted from 575 to 570 cm(-1) (Fe-O(2)), and 504 to 499 cm(-1), respectively, as the result of the replacement of Ile209 with an Ala residue. In the I209A mutant of RmFixL, the O(2) sensing activity was destroyed, thus confirming our proposed mechanism.
-
-	Sensory mechanism of oxygen sensor FixL from Rhizobium meliloti: crystallographic, mutagenesis and resonance Raman spectroscopic studies.,Miyatake H, Mukai M, Park SY, Adachi S, Tamura K, Nakamura H, Nakamura K, Tsuchiya T, Iizuka T, Shiro Y J Mol Biol. 2000 Aug 11;301(2):415-31. PMID:10926518<ref>PMID:10926518</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ew0" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 9930]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adachi, S]]	+	[[Category: Sinorhizobium meliloti]]
-	[[Category: Iizuka, T]]	+	[[Category: Adachi S]]
-	[[Category: Miyatake, H]]	+	[[Category: Iizuka T]]
-	[[Category: Mukai, M]]	+	[[Category: Miyatake H]]
-	[[Category: Nakamura, H]]	+	[[Category: Mukai M]]
-	[[Category: Nakamura, K]]	+	[[Category: Nakamura H]]
-	[[Category: Park, S Y]]	+	[[Category: Nakamura K]]
-	[[Category: Shiro, Y]]	+	[[Category: Park S-Y]]
-	[[Category: Tamura, K]]	+	[[Category: Shiro Y]]
-	[[Category: Tsuchiya, T]]	+	[[Category: Tamura K]]
-	~~[[Category: Heme protein]]~~	+	[[Category: Tsuchiya T]]
-	~~[[Category: Histidine kinase]]~~	+
-	~~[[Category: Oxygen sensor]]~~	+
-	~~[[Category: Rhizobium meliloti]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:42, 21 July 2021

2021-07-21T09:42:34Z

←Older revision		Revision as of 09:42, 21 July 2021
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	<StructureSection load='1ew0' size='340' side='right'caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1ew0' size='340' side='right'caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d06\|1d06]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [~~http~~://pdbe.org/1ew0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ew0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [https://pdbe.org/1ew0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [https://www.ebi.ac.uk/pdbsum/1ew0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FIXL_RHIME FIXL_RHIME]] Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.	+	[[https://www.uniprot.org/uniprot/FIXL_RHIME FIXL_RHIME]] Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:57, 16 October 2019

2019-10-16T13:57:33Z

←Older revision		Revision as of 13:57, 16 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)==		==CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)==
-	<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='1ew0' size='340' side='right'caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 9930]]		[[Category: Atcc 9930]]
		+	[[Category: Large Structures]]
	[[Category: Adachi, S]]		[[Category: Adachi, S]]
	[[Category: Iizuka, T]]		[[Category: Iizuka, T]]

OCA at 07:11, 20 December 2017

2017-12-20T07:11:46Z

←Older revision		Revision as of 07:11, 20 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)==		==CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)==
	<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://pdbe.org/1ew0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://pdbe.org/1ew0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ew0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ew0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ew0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:37, 9 February 2016

2016-02-09T15:37:43Z

←Older revision		Revision as of 15:37, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ew0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:39, 10 September 2015

2015-09-10T23:39:01Z

←Older revision		Revision as of 23:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sinorhizobium_meliloti Sinorhizobium meliloti~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://pdbe.org/1ew0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ew0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sinorhizobium meliloti~~]]	+	[[Category: Atcc 9930]]
	[[Category: Adachi, S]]		[[Category: Adachi, S]]
	[[Category: Iizuka, T]]		[[Category: Iizuka, T]]

OCA at 07:47, 25 December 2014

2014-12-25T07:47:31Z

←Older revision		Revision as of 07:47, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FIXL_RHIME FIXL_RHIME]] Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:47, 22 December 2014

2014-12-22T19:47:28Z

←Older revision		Revision as of 19:47, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Sinorhizobium meliloti]]		[[Category: Sinorhizobium meliloti]]
-	[[Category: Adachi, S.]]	+	[[Category: Adachi, S]]
-	[[Category: Iizuka, T.]]	+	[[Category: Iizuka, T]]
-	[[Category: Miyatake, H.]]	+	[[Category: Miyatake, H]]
-	[[Category: Mukai, M.]]	+	[[Category: Mukai, M]]
-	[[Category: Nakamura, H.]]	+	[[Category: Nakamura, H]]
-	[[Category: Nakamura, K.]]	+	[[Category: Nakamura, K]]
-	[[Category: Park, S Y.]]	+	[[Category: Park, S Y]]
-	[[Category: Shiro, Y.]]	+	[[Category: Shiro, Y]]
-	[[Category: Tamura, K.]]	+	[[Category: Tamura, K]]
-	[[Category: Tsuchiya, T.]]	+	[[Category: Tsuchiya, T]]
	[[Category: Heme protein]]		[[Category: Heme protein]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]

OCA at 14:43, 29 September 2014

2014-09-29T14:43:49Z

←Older revision		Revision as of 14:43, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ew0.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)==
		+	<StructureSection load='1ew0' size='340' side='right' caption='[[1ew0]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ew0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EW0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d06\|1d06]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ew0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ew0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ew0 RCSB], [http://www.ebi.ac.uk/pdbsum/1ew0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1ew0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FixL of Rhizobium meliloti (RmFixL) is a sensor histidine kinase of the two-component system, which regulates the expression of the genes related to nitrogen fixation in the root nodule in response to the O(2) levels. The crystal structure of the sensor domain of FixL (RmFixLH), which contains a heme (Fe-porphyrin) as a sensing site, was determined at 1.4 A resolution. Based on the structural and spectroscopic analyses, we propose the O(2) sensing mechanism that differs from the case proposed in BjFixLH as follows; conformational changes in the F/G loop, which are induced by steric repulsion between the bent-bound O(2) and the Ile209 side-chain, would be transmitted to the histidine kinase domain. Interaction between the iron-bound O(2) and Ile209 was also observed in the resonance Raman spectra of RmFixLH as evidenced by the fact that the Fe-O(2) and Fe-CN stretching frequencies were shifted from 575 to 570 cm(-1) (Fe-O(2)), and 504 to 499 cm(-1), respectively, as the result of the replacement of Ile209 with an Ala residue. In the I209A mutant of RmFixL, the O(2) sensing activity was destroyed, thus confirming our proposed mechanism.

-	~~{{STRUCTURE_1ew0\| PDB=1ew0 \| SCENE= }}~~	+	Sensory mechanism of oxygen sensor FixL from Rhizobium meliloti: crystallographic, mutagenesis and resonance Raman spectroscopic studies.,Miyatake H, Mukai M, Park SY, Adachi S, Tamura K, Nakamura H, Nakamura K, Tsuchiya T, Iizuka T, Shiro Y J Mol Biol. 2000 Aug 11;301(2):415-31. PMID:10926518<ref>PMID:10926518</ref>

-	~~===CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10926518}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ew0]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EW0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010926518<~~/~~ref~~><~~ref group="xtra">PMID:010329771</ref><references group="xtra"~~/>	+
	[[Category: Sinorhizobium meliloti]]		[[Category: Sinorhizobium meliloti]]
	[[Category: Adachi, S.]]		[[Category: Adachi, S.]]

OCA at 09:38, 16 January 2013

2013-01-16T09:38:22Z

←Older revision		Revision as of 09:38, 16 January 2013
Line 1:		Line 1:
	[[Image:1ew0.png\|left\|200px]]		[[Image:1ew0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ew0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ew0\| PDB=1ew0 \| SCENE= }}		{{STRUCTURE_1ew0\| PDB=1ew0 \| SCENE= }}

	===CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)===		===CRYSTAL STRUCTURE ANALYSIS OF THE SENSOR DOMAIN OF RMFIXL(FERROUS FORM)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10926518}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10926518 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10926518}}		{{ABSTRACT_PUBMED_10926518}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10926518~~</ref><ref group="xtra">PMID:~~10329771</ref><ref group="xtra">PMID:10438488~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010926518</ref><ref group="xtra">PMID:010329771</ref><references group="xtra"/>
	[[Category: Sinorhizobium meliloti]]		[[Category: Sinorhizobium meliloti]]
	[[Category: Adachi, S.]]		[[Category: Adachi, S.]]