1ex2 - Revision history

OCA at 10:07, 20 March 2024

2024-03-20T10:07:28Z

←Older revision		Revision as of 10:07, 20 March 2024
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	<StructureSection load='1ex2' size='340' side='right'caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1ex2' size='340' side='right'caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=~~FRU~~:~~FRUCTOSE~~'>~~FRU~~</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [https://pdbe.org/1ex2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex2 ProSAT], [https://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [https://pdbe.org/1ex2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex2 ProSAT], [https://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~MAF_BACSU MAF_BACSU~~]~~] Involved~~ in ~~septum formation~~.	+	[https://www.uniprot.org/uniprot/NTPPA_BACSU NTPPA_BACSU] Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. Can also hydrolyze CTP and the modified nucleotides pseudo-UTP, 5-methyl-CTP (m(5)CTP) and 5-methyl-UTP (m(5)UTP) (PubMed:24210219). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids (PubMed:24210219).<ref>PMID:24210219</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex2 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Three-dimensional structures of functionally uncharacterized proteins may furnish insight into their functions. The potential benefits of three-dimensional structural information regarding such proteins are particularly obvious when the corresponding genes are conserved during evolution, implying an important function, and no functional classification can be inferred from their sequences. The Bacillus subtilis Maf protein is representative of a family of proteins that has homologs in many of the completely sequenced genomes from archaea, prokaryotes, and eukaryotes, but whose function is unknown. As an aid in exploring function, we determined the crystal structure of this protein at a resolution of 1.85 A. The structure, in combination with multiple sequence alignment, reveals a putative active site. Phosphate ions present at this site and structural similarities between a portion of Maf and the anticodon-binding domains of several tRNA synthetases suggest that Maf may be a nucleic acid-binding protein. The crystal structure of a Maf-nucleoside triphosphate complex provides support for this hypothesis and hints at di- or oligonucleotides with either 5'- or 3'-terminal phosphate groups as ligands or substrates of Maf. A further clue comes from the observation that the structure of the Maf monomer bears similarity to that of the recently reported Methanococcus jannaschii Mj0226 protein. Just as for Maf, the structure of this predicted NTPase was determined as part of a structural genomics pilot project. The structural relation between Maf and Mj0226 was not apparent from sequence analysis approaches. These results emphasize the potential of structural genomics to reveal new unexpected connections between protein families previously considered unrelated.
-
-	Functional implications from crystal structures of the conserved Bacillus subtilis protein Maf with and without dUTP.,Minasov G, Teplova M, Stewart GC, Koonin EV, Anderson WF, Egli M Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6328-33. PMID:10841541<ref>PMID:10841541</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ex2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anderson~~, W F~~]]	+	[[Category: Anderson WF]]
-	[[Category: Egli, M]]	+	[[Category: Egli M]]
-	[[Category: Koonin~~, E V]]~~	+	[[Category: Koonin EV]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Stewart GC]]
-	[[Category: Stewart~~, G C~~]]	+	[[Category: Teplova M]]
-	[[Category: Teplova, M]]	+
-	~~[[Category: B. subtilis protein maf]]~~	+
-	~~[[Category: Mcsg]]~~	+
-	~~[[Category: PSI, Protein structure initiative~~]]	+

OCA at 09:43, 21 July 2021

2021-07-21T09:43:08Z

←Older revision		Revision as of 09:43, 21 July 2021
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	<StructureSection load='1ex2' size='340' side='right'caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1ex2' size='340' side='right'caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=~~SUC~~:~~SUCROSE~~'>~~SUC~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [~~http~~://pdbe.org/1ex2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex2 ProSAT], [~~http~~://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [https://pdbe.org/1ex2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex2 ProSAT], [https://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.	+	[[https://www.uniprot.org/uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:02, 16 October 2019

2019-10-16T11:02:26Z

←Older revision		Revision as of 11:02, 16 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN==		==CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN==
-	<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='1ex2' size='340' side='right'caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Egli, M]]		[[Category: Egli, M]]

OCA at 07:18, 20 December 2017

2017-12-20T07:18:17Z

←Older revision		Revision as of 07:18, 20 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN==		==CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN==
	<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://pdbe.org/1ex2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://pdbe.org/1ex2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex2 ProSAT], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Egli, M]]		[[Category: Egli, M]]

OCA at 14:49, 7 February 2016

2016-02-07T14:49:30Z

←Older revision		Revision as of 14:49, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:31, 10 September 2015

2015-09-10T02:31:51Z

←Older revision		Revision as of 02:31, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://pdbe.org/1ex2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ex2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Egli, M]]		[[Category: Egli, M]]

OCA at 16:01, 24 December 2014

2014-12-24T16:01:52Z

←Older revision		Revision as of 16:01, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:37, 22 December 2014

2014-12-22T23:37:16Z

←Older revision		Revision as of 23:37, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: Egli, M.]]	+	[[Category: Egli, M]]
-	[[Category: Koonin, E V.]]	+	[[Category: Koonin, E V]]
-	[[Category: ~~MCSG, Midwest Center for~~ Structural ~~Genomics.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Minasov, G.]]	+	[[Category: Minasov, G]]
-	[[Category: Stewart, G C.]]	+	[[Category: Stewart, G C]]
-	[[Category: Teplova, M.]]	+	[[Category: Teplova, M]]
	[[Category: B. subtilis protein maf]]		[[Category: B. subtilis protein maf]]
	[[Category: Mcsg]]		[[Category: Mcsg]]
-	[[Category: ~~Midwest center for structural genomic]]~~	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category:~~ Protein structure initiative]]	+
-	~~[[Category: Psi]]~~	+
-	~~[[Category: Structural genomic~~]]	+

OCA at 11:10, 3 October 2014

2014-10-03T11:10:48Z

←Older revision		Revision as of 11:10, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1ex2\| PDB=1ex2 \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN==
-	===CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN===	+	<StructureSection load='1ex2' size='340' side='right' caption='[[1ex2]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~{{ABSTRACT_PUBMED_10841541}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex2 PDBsum], [http://www.topsan.org/Proteins/MCSG/1ex2 TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Three-dimensional structures of functionally uncharacterized proteins may furnish insight into their functions. The potential benefits of three-dimensional structural information regarding such proteins are particularly obvious when the corresponding genes are conserved during evolution, implying an important function, and no functional classification can be inferred from their sequences. The Bacillus subtilis Maf protein is representative of a family of proteins that has homologs in many of the completely sequenced genomes from archaea, prokaryotes, and eukaryotes, but whose function is unknown. As an aid in exploring function, we determined the crystal structure of this protein at a resolution of 1.85 A. The structure, in combination with multiple sequence alignment, reveals a putative active site. Phosphate ions present at this site and structural similarities between a portion of Maf and the anticodon-binding domains of several tRNA synthetases suggest that Maf may be a nucleic acid-binding protein. The crystal structure of a Maf-nucleoside triphosphate complex provides support for this hypothesis and hints at di- or oligonucleotides with either 5'- or 3'-terminal phosphate groups as ligands or substrates of Maf. A further clue comes from the observation that the structure of the Maf monomer bears similarity to that of the recently reported Methanococcus jannaschii Mj0226 protein. Just as for Maf, the structure of this predicted NTPase was determined as part of a structural genomics pilot project. The structural relation between Maf and Mj0226 was not apparent from sequence analysis approaches. These results emphasize the potential of structural genomics to reveal new unexpected connections between protein families previously considered unrelated.

-	~~==Function==~~	+	Functional implications from crystal structures of the conserved Bacillus subtilis protein Maf with and without dUTP.,Minasov G, Teplova M, Stewart GC, Koonin EV, Anderson WF, Egli M Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6328-33. PMID:10841541<ref>PMID:10841541</ref>
-	~~[[http~~:~~//www~~.~~uniprot.org~~/~~uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.~~	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1ex2]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:010841541</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 10:57, 16 April 2014

2014-04-16T10:57:40Z

←Older revision		Revision as of 10:57, 16 April 2014
Line 1:		Line 1:
-	[[Image:1ex2.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ex2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ex2\| PDB=1ex2 \| SCENE= }}		{{STRUCTURE_1ex2\| PDB=1ex2 \| SCENE= }}
-
	===CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN===		===CRYSTAL STRUCTURE OF BACILLUS SUBTILIS MAF PROTEIN===
		+	{{ABSTRACT_PUBMED_10841541}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/MAF_BACSU MAF_BACSU]] Involved in septum formation.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_10841541}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 10841541 is the PubMed ID number~~.	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_10841541}}~~	+

	==About this Structure==		==About this Structure==
	[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA].		[[1ex2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX2 OCA].
-
-	==See Also==
-	*[[User:Adrian Aldrich/Section 1\|User:Adrian Aldrich/Section 1]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:010841541</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010841541</ref><references group="xtra"/><references/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]