1fkq - Revision history

OCA at 11:13, 27 March 2024

2024-03-27T11:13:41Z

←Older revision		Revision as of 11:13, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1fkq' size='340' side='right'caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1fkq' size='340' side='right'caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~African_dwarf_goat African dwarf goat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1hmk\|1hmk]]~~, [[~~1hfy~~\|~~1hfy~~]]~~, [[1fkv\|1fkv]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>A CLONED GENE OF GOAT LACTA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9925 African dwarf goat])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [https://pdbe.org/1fkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [https://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [https://pdbe.org/1fkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [https://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.	+	[https://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 22:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fkq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fkq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Thr29 residue in the hydrophobic core of goat alpha-lactalbumin (alpha-LA) was substituted with Val (Thr29Val) and Ile (Thr29Ile) to investigate the contribution of Thr29 to the thermodynamic stability of the protein. We carried out protein stability measurements, X-ray crystallographic analyses, and free energy calculations based on molecular dynamics simulation. The equilibrium unfolding transitions induced by guanidine hydrochloride demonstrated that the Thr29Val and Thr29Ile mutants were, respectively, 1.9 and 3.2 kcal/mol more stable than the wild-type protein (WT). The overall structures of the mutants were almost identical to that of WT, in spite of the disruption of the hydrogen bonding between the side-chain O-H group of Thr29 and the main-chain C=O group of Glu25. To analyze the stabilization mechanism of the mutants, we performed free energy calculations. The calculated free energy differences were in good agreement with the experimental values. The stabilization of the mutants was mainly caused by solvation loss in the denatured state. Furthermore, the O-H group of Thr29 favorably interacts with the C=O group of Glu25 to form hydrogen bonds and, simultaneously, unfavorably interacts electrostatically with the main-chain C=O group of Thr29. The difference in the free energy profile of the unfolding path between WT and the Thr29Ile mutant is discussed in light of our experimental and theoretical results.
-
-	Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.,Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I Proteins. 2001 Oct 1;45(1):16-29. PMID:11536356<ref>PMID:11536356</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Alpha-lactalbumin 3D structures\|Alpha-lactalbumin 3D structures]]		*[[Alpha-lactalbumin 3D structures\|Alpha-lactalbumin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~African dwarf goat]]~~	+	[[Category: Capra hircus]]
-	~~[[Category: Lactose synthase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Horii, K]]	+	[[Category: Horii K]]
-	[[Category: Kumagai, I]]	+	[[Category: Kumagai I]]
-	[[Category: Matsushima, M]]	+	[[Category: Matsushima M]]
-	[[Category: Tsumoto, K]]	+	[[Category: Tsumoto K]]
-	~~[[Category: Calcium binding metalloprotein]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Lactose synthase component]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 15:12, 3 November 2021

2021-11-03T15:12:50Z

OCA at 10:26, 18 November 2020

2020-11-18T10:26:05Z

←Older revision		Revision as of 10:26, 18 November 2020
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	==RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V==		==RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V==
-	<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1fkq' size='340' side='right'caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://pdbe.org/1fkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://pdbe.org/1fkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alpha-lactalbumin 3D structures\|Alpha-lactalbumin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 40:
	[[Category: African dwarf goat]]		[[Category: African dwarf goat]]
	[[Category: Lactose synthase]]		[[Category: Lactose synthase]]
		+	[[Category: Large Structures]]
	[[Category: Horii, K]]		[[Category: Horii, K]]
	[[Category: Kumagai, I]]		[[Category: Kumagai, I]]

OCA at 09:25, 27 December 2017

2017-12-27T09:25:45Z

←Older revision		Revision as of 09:25, 27 December 2017
Line 1:		Line 1:
		+
	==RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V==		==RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V==
	<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://pdbe.org/1fkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://pdbe.org/1fkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/1fkq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/1fkq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Lactalbumin\|Lactalbumin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:35, 9 February 2016

2016-02-09T06:35:49Z

←Older revision		Revision as of 06:35, 9 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fkq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:34, 10 September 2015

2015-09-10T19:34:02Z

←Older revision		Revision as of 19:34, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Capra_hircus Capra hircus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 ~~Capra hircus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://pdbe.org/1fkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fkq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Capra hircus~~]]	+	[[Category: African dwarf goat]]
	[[Category: Lactose synthase]]		[[Category: Lactose synthase]]
	[[Category: Horii, K]]		[[Category: Horii, K]]

OCA at 05:05, 25 December 2014

2014-12-25T05:05:10Z

←Older revision		Revision as of 05:05, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 22:16, 22 December 2014

2014-12-22T22:16:42Z

←Older revision		Revision as of 22:16, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 Capra hircus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 Capra hircus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Capra hircus]]		[[Category: Capra hircus]]
	[[Category: Lactose synthase]]		[[Category: Lactose synthase]]
-	[[Category: Horii, K.]]	+	[[Category: Horii, K]]
-	[[Category: Kumagai, I.]]	+	[[Category: Kumagai, I]]
-	[[Category: Matsushima, M.]]	+	[[Category: Matsushima, M]]
-	[[Category: Tsumoto, K.]]	+	[[Category: Tsumoto, K]]
	[[Category: Calcium binding metalloprotein]]		[[Category: Calcium binding metalloprotein]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Lactose synthase component]]		[[Category: Lactose synthase component]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 12:07, 28 September 2014

2014-09-28T12:07:59Z

←Older revision		Revision as of 12:07, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1fkq.~~png~~\|~~left~~\|200px]]	+	==RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V==
		+	<StructureSection load='1fkq' size='340' side='right' caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 Capra hircus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [http://www.ebi.ac.uk/pdbsum/1fkq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/1fkq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Thr29 residue in the hydrophobic core of goat alpha-lactalbumin (alpha-LA) was substituted with Val (Thr29Val) and Ile (Thr29Ile) to investigate the contribution of Thr29 to the thermodynamic stability of the protein. We carried out protein stability measurements, X-ray crystallographic analyses, and free energy calculations based on molecular dynamics simulation. The equilibrium unfolding transitions induced by guanidine hydrochloride demonstrated that the Thr29Val and Thr29Ile mutants were, respectively, 1.9 and 3.2 kcal/mol more stable than the wild-type protein (WT). The overall structures of the mutants were almost identical to that of WT, in spite of the disruption of the hydrogen bonding between the side-chain O-H group of Thr29 and the main-chain C=O group of Glu25. To analyze the stabilization mechanism of the mutants, we performed free energy calculations. The calculated free energy differences were in good agreement with the experimental values. The stabilization of the mutants was mainly caused by solvation loss in the denatured state. Furthermore, the O-H group of Thr29 favorably interacts with the C=O group of Glu25 to form hydrogen bonds and, simultaneously, unfavorably interacts electrostatically with the main-chain C=O group of Thr29. The difference in the free energy profile of the unfolding path between WT and the Thr29Ile mutant is discussed in light of our experimental and theoretical results.

-	~~{{STRUCTURE_1fkq\| PDB=1fkq \| SCENE= }}~~	+	Contribution of Thr29 to the thermodynamic stability of goat alpha-lactalbumin as determined by experimental and theoretical approaches.,Horii K, Saito M, Yoda T, Tsumoto K, Matsushima M, Kuwajima K, Kumagai I Proteins. 2001 Oct 1;45(1):16-29. PMID:11536356<ref>PMID:11536356</ref>

-	~~===RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11536356}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1fkq]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Capra_hircus Capra hircus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA].~~	+

	==See Also==		==See Also==
	*[[Lactalbumin\|Lactalbumin]]		*[[Lactalbumin\|Lactalbumin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011536356<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Capra hircus]]		[[Category: Capra hircus]]
	[[Category: Lactose synthase]]		[[Category: Lactose synthase]]

OCA at 12:59, 20 October 2012

2012-10-20T12:59:11Z

←Older revision		Revision as of 12:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1fkq]] is a 1 chain structure ~~of [[Lactalbumin]]~~ with sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA].	+	[[1fkq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Capra_hircus Capra hircus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA].

	==See Also==		==See Also==

←Older revision		Revision as of 15:12, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='1fkq' size='340' side='right'caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1fkq' size='340' side='right'caption='[[1fkq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fkq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/African_dwarf_goat African dwarf goat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hmk\|1hmk]], [[1hfy\|1hfy]], [[1fkv\|1fkv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">A CLONED GENE OF GOAT LACTA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9925 African dwarf goat])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactose_synthase Lactose synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.22 2.4.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [~~http~~://pdbe.org/1fkq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fkq OCA], [https://pdbe.org/1fkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fkq RCSB], [https://www.ebi.ac.uk/pdbsum/1fkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fkq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.	+	[[https://www.uniprot.org/uniprot/LALBA_CAPHI LALBA_CAPHI]] Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]