1fx8 - Revision history

OCA at 07:20, 7 February 2024

2024-02-07T07:20:13Z

←Older revision		Revision as of 07:20, 7 February 2024
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	<StructureSection load='1fx8' size='340' side='right'caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fx8' size='340' side='right'caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. The May 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The May 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [https://pdbe.org/1fx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fx8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [https://pdbe.org/1fx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fx8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GLPF_ECOLI GLPF_ECOLI]] Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.	+	[https://www.uniprot.org/uniprot/GLPF_ECOLI GLPF_ECOLI] Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fx8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fx8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Membrane channel proteins of the aquaporin family are highly selective for permeation of specific small molecules, with absolute exclusion of ions and charged solutes and without dissipation of the electrochemical potential across the cell membrane. We report the crystal structure of the Escherichia coli glycerol facilitator (GlpF) with its primary permeant substrate glycerol at 2.2 angstrom resolution. Glycerol molecules line up in an amphipathic channel in single file. In the narrow selectivity filter of the channel the glycerol alkyl backbone is wedged against a hydrophobic corner, and successive hydroxyl groups form hydrogen bonds with a pair of acceptor, and donor atoms. Two conserved aspartic acid-proline-alanine motifs form a key interface between two gene-duplicated segments that each encode three-and-one-half membrane-spanning helices around the channel. This structure elucidates the mechanism of selective permeability for linear carbohydrates and suggests how ions and water are excluded.
-
-	Structure of a glycerol-conducting channel and the basis for its selectivity.,Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM Science. 2000 Oct 20;290(5491):481-6. PMID:11039922<ref>PMID:11039922</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fx8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Aquaporin 3D structures\|Aquaporin 3D structures]]		*[[Aquaporin 3D structures\|Aquaporin 3D structures]]
	*[[Ion channels 3D structures\|Ion channels 3D structures]]		*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Aquaporin]]		[[Category: Aquaporin]]
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Fu, D]]	+	[[Category: Fu D]]
-	[[Category: Libson, A]]	+	[[Category: Libson A]]
-	[[Category: Miercke~~, L J.W~~]]	+	[[Category: Miercke LJW]]
-	[[Category: Nollert, P]]	+	[[Category: Nollert P]]
-	[[Category: Stroud~~, R M~~]]	+	[[Category: Stroud RM]]
-	[[Category: Weitzman, C]]	+	[[Category: Weitzman C]]
-	~~[[Category: Glycerol-conducting membrane channel protein]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 07:09, 24 March 2021

2021-03-24T07:09:27Z

←Older revision		Revision as of 07:09, 24 March 2021
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	<StructureSection load='1fx8' size='340' side='right'caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fx8' size='340' side='right'caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure. The May 2014 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. The May 2014 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [~~http~~://pdbe.org/1fx8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fx8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fx8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [https://pdbe.org/1fx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fx8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLPF_ECOLI GLPF_ECOLI]] Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.	+	[[https://www.uniprot.org/uniprot/GLPF_ECOLI GLPF_ECOLI]] Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[Aquaporin 3D structures\|Aquaporin 3D structures]]		*[[Aquaporin 3D structures\|Aquaporin 3D structures]]
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Aquaporin]]		[[Category: Aquaporin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 07:11, 19 June 2019

2019-06-19T07:11:52Z

←Older revision		Revision as of 07:11, 19 June 2019
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	==CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL==		==CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL==
-	<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1fx8' size='340' side='right'caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure. The May 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure. The May 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/1fx8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/1fx8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Aquaporin\|Aquaporin]]	+	*[[Aquaporin 3D structures\|Aquaporin 3D structures]]
	*[[Ion channels\|Ion channels]]		*[[Ion channels\|Ion channels]]
	== References ==		== References ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Aquaporin]]		[[Category: Aquaporin]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Fu, D]]		[[Category: Fu, D]]

OCA at 11:00, 13 September 2017

2017-09-13T11:00:22Z

←Older revision		Revision as of 11:00, 13 September 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL==		==CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL==
	<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure ~~with sequence from~~ [http://en.~~wikipedia~~.org/~~wiki~~/~~"bacillus_coli"_migula_1895 "bacillus coli" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure. The May 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aquaporin'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_5 10.2210/rcsb_pdb/mom_2014_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://pdbe.org/1fx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://pdbe.org/1fx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fx8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fx8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus coli migula 1895~~]]	+	[[Category: Aquaporin]]
		+	[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Fu, D]]		[[Category: Fu, D]]
	[[Category: Libson, A]]		[[Category: Libson, A]]

OCA at 13:01, 11 September 2015

2015-09-11T13:01:39Z

←Older revision		Revision as of 13:01, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://pdbe.org/1fx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fx8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Fu, D]]		[[Category: Fu, D]]
	[[Category: Libson, A]]		[[Category: Libson, A]]

OCA at 16:48, 25 December 2014

2014-12-25T16:48:07Z

←Older revision		Revision as of 16:48, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLPF_ECOLI GLPF_ECOLI]] Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 21:18, 22 December 2014

2014-12-22T21:18:51Z

←Older revision		Revision as of 21:18, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Fu, D.]]	+	[[Category: Fu, D]]
-	[[Category: Libson, A.]]	+	[[Category: Libson, A]]
-	[[Category: Miercke, L J.W.]]	+	[[Category: Miercke, L J.W]]
-	[[Category: Nollert, P.]]	+	[[Category: Nollert, P]]
-	[[Category: Stroud, R M.]]	+	[[Category: Stroud, R M]]
-	[[Category: Weitzman, C.]]	+	[[Category: Weitzman, C]]
	[[Category: Glycerol-conducting membrane channel protein]]		[[Category: Glycerol-conducting membrane channel protein]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 11:09, 28 September 2014

2014-09-28T11:09:59Z

←Older revision		Revision as of 11:09, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1fx8.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL==
		+	<StructureSection load='1fx8' size='340' side='right' caption='[[1fx8]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FX8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fx8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fx8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fx/1fx8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane channel proteins of the aquaporin family are highly selective for permeation of specific small molecules, with absolute exclusion of ions and charged solutes and without dissipation of the electrochemical potential across the cell membrane. We report the crystal structure of the Escherichia coli glycerol facilitator (GlpF) with its primary permeant substrate glycerol at 2.2 angstrom resolution. Glycerol molecules line up in an amphipathic channel in single file. In the narrow selectivity filter of the channel the glycerol alkyl backbone is wedged against a hydrophobic corner, and successive hydroxyl groups form hydrogen bonds with a pair of acceptor, and donor atoms. Two conserved aspartic acid-proline-alanine motifs form a key interface between two gene-duplicated segments that each encode three-and-one-half membrane-spanning helices around the channel. This structure elucidates the mechanism of selective permeability for linear carbohydrates and suggests how ions and water are excluded.

-	~~{{STRUCTURE_1fx8\| PDB=1fx8 \| SCENE= }}~~	+	Structure of a glycerol-conducting channel and the basis for its selectivity.,Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM Science. 2000 Oct 20;290(5491):481-6. PMID:11039922<ref>PMID:11039922</ref>

-	~~===CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11039922}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1fx8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA].~~	+

	==See Also==		==See Also==
		+	*[[Aquaporin\|Aquaporin]]
	*[[Ion channels\|Ion channels]]		*[[Ion channels\|Ion channels]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011039922<~~/~~ref~~><~~ref group="xtra">PMID:015181215</ref><ref group="xtra">PMID:016792816</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Fu, D.]]		[[Category: Fu, D.]]

OCA at 19:05, 20 October 2012

2012-10-20T19:05:40Z

←Older revision		Revision as of 19:05, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1fx8]] is a 1 chain structure ~~of [[Ion channels]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA].	+	[[1fx8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX8 OCA].

	==See Also==		==See Also==

OCA at 06:16, 27 July 2012

2012-07-27T06:16:11Z

←Older revision		Revision as of 06:16, 27 July 2012
Line 1:		Line 1:
	[[Image:1fx8.png\|left\|200px]]		[[Image:1fx8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1fx8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1fx8\| PDB=1fx8 \| SCENE= }}		{{STRUCTURE_1fx8\| PDB=1fx8 \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL===		===CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11039922}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11039922 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11039922}}		{{ABSTRACT_PUBMED_11039922}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Ion channels]]	+	*[[Ion channels\|Ion channels]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11039922~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011039922</ref><ref group="xtra">PMID:015181215</ref><ref group="xtra">PMID:016792816</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Fu, D.]]		[[Category: Fu, D.]]
Line 33:		Line 22:
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
	[[Category: Weitzman, C.]]		[[Category: Weitzman, C.]]
		+	[[Category: Glycerol-conducting membrane channel protein]]
		+	[[Category: Membrane protein]]