1gsh - Revision history

OCA at 07:26, 7 February 2024

2024-02-07T07:26:25Z

←Older revision		Revision as of 07:26, 7 February 2024
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	<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Escherichia_coli_(strain_b)~~ Escherichia coli ~~(strain b)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_B Escherichia coli B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.~~2~~.3 6.3.2.3] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [https://pdbe.org/1gsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [https://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [https://pdbe.org/1gsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [https://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GSHB_ECOLI GSHB_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsh ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of Escherichia coli B glutathione synthetase (GSHase) has been determined at the optimal catalytic condition pH 7.5. The most significant structural difference from the structure at pH 6.0 is the movement of the central domain towards the N-terminal domain almost as a rigid body. As a result of this movement, new interdomain and intersubunit polar interactions are formed which stabilize the dimeric structure further. The structure of GSHase at optimal pH was compared with 294 other known protein structures in terms of the spatial arrangements of secondary structural elements. Three enzymes (D-alanine: D-alanine ligase, succinyl-CoA synthetase and the biotin carboxylase subunit of acetyl-CoA carboxylase) were found to have structures similar to the ATP-binding site of GSHase, which extends across two domains. The ATP-binding sites in these four enzymes are composed of two antiparallel beta-sheets and are different from the classic mononucleotide-binding fold. Except for these proteins, no significant structural similarity was detected between GSHase and the other ATP-binding proteins. A structural motif in the N-terminal domain of GSHase has been found to be similar to the NAD-binding fold. This structural motif is shared by a number of other proteins that bind various negatively charged molecules.
-
-	Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins.,Matsuda K, Mizuguchi K, Nishioka T, Kato H, Go N, Oda J Protein Eng. 1996 Dec;9(12):1083-92. PMID:9010922<ref>PMID:9010922</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glutathione synthetase\|Glutathione synthetase]]		*[[Glutathione synthetase\|Glutathione synthetase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutathione synthase~~]]	+	[[Category: Escherichia coli B]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kato, H]]	+	[[Category: Kato H]]
-	[[Category: Katsube, Y]]	+	[[Category: Katsube Y]]
-	[[Category: Matsuda, K]]	+	[[Category: Matsuda K]]
-	[[Category: Nishioka, T]]	+	[[Category: Nishioka T]]
-	[[Category: Oda, J]]	+	[[Category: Oda J]]
-	[[Category: Yamaguchi, H]]	+	[[Category: Yamaguchi H]]
-	~~[[Category: Glutathione biosynthesis]]~~	+
-	~~[[Category: Glutathione biosynthesis ligase~~]]	+

OCA at 11:28, 28 July 2021

2021-07-28T11:28:01Z

←Older revision		Revision as of 11:28, 28 July 2021
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	<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [~~http~~://pdbe.org/1gsh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [https://pdbe.org/1gsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [https://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:05, 23 October 2019

2019-10-23T09:05:47Z

←Older revision		Revision as of 09:05, 23 October 2019
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	==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==		==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==
-	<StructureSection load='1gsh' size='340' side='right' caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1gsh' size='340' side='right'caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione synthetase\|Glutathione synthetase]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Glutathione synthase]]		[[Category: Glutathione synthase]]
		+	[[Category: Large Structures]]
	[[Category: Kato, H]]		[[Category: Kato, H]]
	[[Category: Katsube, Y]]		[[Category: Katsube, Y]]

OCA at 06:45, 3 January 2018

2018-01-03T06:45:05Z

←Older revision		Revision as of 06:45, 3 January 2018
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		+
	==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==		==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==
	<StructureSection load='1gsh' size='340' side='right' caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1gsh' size='340' side='right' caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://pdbe.org/1gsh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://pdbe.org/1gsh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gsh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glutathione synthetase\|Glutathione synthetase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:21, 8 February 2016

2016-02-08T01:21:38Z

←Older revision		Revision as of 01:21, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gsh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:33, 10 September 2015

2015-09-10T06:33:46Z

←Older revision		Revision as of 06:33, 10 September 2015
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	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://pdbe.org/1gsh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gsh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 21:31, 22 December 2014

2014-12-22T21:31:54Z

←Older revision		Revision as of 21:31, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Glutathione synthase]]		[[Category: Glutathione synthase]]
-	[[Category: Kato, H.]]	+	[[Category: Kato, H]]
-	[[Category: Katsube, Y.]]	+	[[Category: Katsube, Y]]
-	[[Category: Matsuda, K.]]	+	[[Category: Matsuda, K]]
-	[[Category: Nishioka, T.]]	+	[[Category: Nishioka, T]]
-	[[Category: Oda, J.]]	+	[[Category: Oda, J]]
-	[[Category: Yamaguchi, H.]]	+	[[Category: Yamaguchi, H]]
	[[Category: Glutathione biosynthesis]]		[[Category: Glutathione biosynthesis]]
	[[Category: Glutathione biosynthesis ligase]]		[[Category: Glutathione biosynthesis ligase]]
-	[[Category: Glutathione synthase]]

OCA at 05:33, 3 October 2014

2014-10-03T05:33:54Z

←Older revision		Revision as of 05:33, 3 October 2014
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-	~~{{STRUCTURE_1gsh\| PDB=1gsh \| SCENE= }}~~	+	==STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5==
-	===STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5===	+	<StructureSection load='1gsh' size='340' side='right' caption='[[1gsh]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_9010922}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GSH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_synthase Glutathione synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.3 6.3.2.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gsh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gsh RCSB], [http://www.ebi.ac.uk/pdbsum/1gsh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gsh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of Escherichia coli B glutathione synthetase (GSHase) has been determined at the optimal catalytic condition pH 7.5. The most significant structural difference from the structure at pH 6.0 is the movement of the central domain towards the N-terminal domain almost as a rigid body. As a result of this movement, new interdomain and intersubunit polar interactions are formed which stabilize the dimeric structure further. The structure of GSHase at optimal pH was compared with 294 other known protein structures in terms of the spatial arrangements of secondary structural elements. Three enzymes (D-alanine: D-alanine ligase, succinyl-CoA synthetase and the biotin carboxylase subunit of acetyl-CoA carboxylase) were found to have structures similar to the ATP-binding site of GSHase, which extends across two domains. The ATP-binding sites in these four enzymes are composed of two antiparallel beta-sheets and are different from the classic mononucleotide-binding fold. Except for these proteins, no significant structural similarity was detected between GSHase and the other ATP-binding proteins. A structural motif in the N-terminal domain of GSHase has been found to be similar to the NAD-binding fold. This structural motif is shared by a number of other proteins that bind various negatively charged molecules.

-	~~==About this Structure==~~	+	Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins.,Matsuda K, Mizuguchi K, Nishioka T, Kato H, Go N, Oda J Protein Eng. 1996 Dec;9(12):1083-92. PMID:9010922<ref>PMID:9010922</ref>
-	~~[[1gsh]] is a 1 chain~~ structure with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_~~(~~strain_b~~) ~~Escherichia coli (strain b)]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1GSH OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Glutathione synthetase\|Glutathione synthetase]]		*[[Glutathione synthetase\|Glutathione synthetase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:009010922</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glutathione synthase]]		[[Category: Glutathione synthase]]
	[[Category: Kato, H.]]		[[Category: Kato, H.]]

OCA at 14:47, 19 February 2014

2014-02-19T14:47:22Z

←Older revision		Revision as of 14:47, 19 February 2014
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-	[[Image:1gsh.png\|left\|200px]]
-
	{{STRUCTURE_1gsh\| PDB=1gsh \| SCENE= }}		{{STRUCTURE_1gsh\| PDB=1gsh \| SCENE= }}
-
	===STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5===		===STRUCTURE OF ESCHERICHIA COLI GLUTATHIONE SYNTHETASE AT PH 7.5===
-
	{{ABSTRACT_PUBMED_9010922}}		{{ABSTRACT_PUBMED_9010922}}

	==About this Structure==		==About this Structure==
-	[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA].	+	[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_(strain_b) Escherichia coli (strain b)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA].

	==See Also==		==See Also==
Line 14:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:009010922</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009010922</ref><references group="xtra"/><references/>
-	~~[[Category: Escherichia coli]]~~	+
	[[Category: Glutathione synthase]]		[[Category: Glutathione synthase]]
	[[Category: Kato, H.]]		[[Category: Kato, H.]]

OCA at 08:26, 20 October 2012

2012-10-20T08:26:02Z

←Older revision		Revision as of 08:26, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1gsh]] is a 1 chain structure ~~of [[Glutathione synthetase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA].	+	[[1gsh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GSH OCA].

	==See Also==		==See Also==