1h4d - Revision history

OCA at 12:16, 13 December 2023

2023-12-13T12:16:58Z

←Older revision		Revision as of 12:16, 13 December 2023
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	<StructureSection load='1h4d' size='340' side='right'caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='1h4d' size='340' side='right'caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>~~, ~~<scene name='pdbligand=LI:LITHIUM+ION'>LI</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1e5k\|1e5k]]~~, ~~[[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [https://pdbe.org/1h4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [https://www.ebi.ac.uk/pdbsum/1h4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [https://pdbe.org/1h4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [https://www.ebi.ac.uk/pdbsum/1h4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MOBA_ECOLI MOBA_ECOLI]] Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized.<ref>PMID:8020507</ref> <ref>PMID:1648082</ref> <ref>PMID:10978348</ref> <ref>PMID:21081498</ref>	+	[https://www.uniprot.org/uniprot/MOBA_ECOLI MOBA_ECOLI] Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized.<ref>PMID:8020507</ref> <ref>PMID:1648082</ref> <ref>PMID:10978348</ref> <ref>PMID:21081498</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Buchanan, G]]	+	[[Category: Buchanan G]]
-	[[Category: Guse, A]]	+	[[Category: Guse A]]
-	[[Category: Kuper, J]]	+	[[Category: Kuper J]]
-	[[Category: Lawson~~, D M~~]]	+	[[Category: Lawson DM]]
-	[[Category: Mendel~~, R R~~]]	+	[[Category: Mendel RR]]
-	[[Category: Palmer, T]]	+	[[Category: Palmer T]]
-	[[Category: Schwarz, G]]	+	[[Category: Schwarz G]]
-	[[Category: Stevenson~~, C E.M]]~~	+	[[Category: Stevenson CEM]]
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Molybdenum cofactor biosynthesis]]~~	+
-	~~[[Category: Molybdopterin nucleotidyl-transferase~~]]	+

OCA at 10:25, 17 February 2021

2021-02-17T10:25:27Z

←Older revision		Revision as of 10:25, 17 February 2021
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	<StructureSection load='1h4d' size='340' side='right'caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='1h4d' size='340' side='right'caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1H4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [~~http~~://pdbe.org/1h4d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1h4d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [https://pdbe.org/1h4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [https://www.ebi.ac.uk/pdbsum/1h4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MOBA_ECOLI MOBA_ECOLI]] Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized.<ref>PMID:8020507</ref> <ref>PMID:1648082</ref> <ref>PMID:10978348</ref> <ref>PMID:21081498</ref>	+	[[https://www.uniprot.org/uniprot/MOBA_ECOLI MOBA_ECOLI]] Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized.<ref>PMID:8020507</ref> <ref>PMID:1648082</ref> <ref>PMID:10978348</ref> <ref>PMID:21081498</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:17, 3 April 2019

2019-04-03T07:17:54Z

←Older revision		Revision as of 07:17, 3 April 2019
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	==Biochemical and Structural Analysis of the Molybdenum Cofactor Biosynthesis protein MobA==		==Biochemical and Structural Analysis of the Molybdenum Cofactor Biosynthesis protein MobA==
-	<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>	+	<StructureSection load='1h4d' size='340' side='right'caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4d_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Buchanan, G]]		[[Category: Buchanan, G]]
	[[Category: Guse, A]]		[[Category: Guse, A]]

OCA at 04:35, 21 September 2017

2017-09-21T04:35:41Z

←Older revision		Revision as of 04:35, 21 September 2017
Line 1:		Line 1:
-	==~~BIOCHEMICAL AND STRUCTURAL ANALYSIS OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA~~==	+
		+	==Biochemical and Structural Analysis of the Molybdenum Cofactor Biosynthesis protein MobA==
	<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://pdbe.org/1h4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://pdbe.org/1h4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Buchanan, G]]		[[Category: Buchanan, G]]
	[[Category: Guse, A]]		[[Category: Guse, A]]

OCA at 12:24, 11 September 2015

2015-09-11T12:24:38Z

←Older revision		Revision as of 12:24, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://pdbe.org/1h4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1h4d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Buchanan, G]]		[[Category: Buchanan, G]]
	[[Category: Guse, A]]		[[Category: Guse, A]]

OCA at 16:24, 25 December 2014

2014-12-25T16:24:17Z

←Older revision		Revision as of 16:24, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MOBA_ECOLI MOBA_ECOLI]] Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor. Is also involved in the biosynthesis of the bis-MGD form of the Moco cofactor (Mo-bisMGD) in which the metal is symmetrically ligated by the dithiolene groups of two MGD molecules. Is necessary and sufficient for the in vitro activation of the DMSOR molybdoenzyme that uses the Mo-bisMGD form of molybdenum cofactor, which implies formation and efficient insertion of the cofactor into the enzyme without the need of a chaperone. Is specific for GTP since other nucleotides such as ATP and GMP can not be utilized.<ref>PMID:8020507</ref> <ref>PMID:1648082</ref> <ref>PMID:10978348</ref> <ref>PMID:21081498</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 20:37, 22 December 2014

2014-12-22T20:37:23Z

←Older revision		Revision as of 20:37, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Buchanan, G.]]	+	[[Category: Buchanan, G]]
-	[[Category: Guse, A.]]	+	[[Category: Guse, A]]
-	[[Category: Kuper, J.]]	+	[[Category: Kuper, J]]
-	[[Category: Lawson, D M.]]	+	[[Category: Lawson, D M]]
-	[[Category: Mendel, R R.]]	+	[[Category: Mendel, R R]]
-	[[Category: Palmer, T.]]	+	[[Category: Palmer, T]]
-	[[Category: Schwarz, G.]]	+	[[Category: Schwarz, G]]
-	[[Category: Stevenson, C E.M.]]	+	[[Category: Stevenson, C E.M]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]
	[[Category: Molybdenum cofactor biosynthesis]]		[[Category: Molybdenum cofactor biosynthesis]]
	[[Category: Molybdopterin nucleotidyl-transferase]]		[[Category: Molybdopterin nucleotidyl-transferase]]

OCA at 10:28, 28 September 2014

2014-09-28T10:28:59Z

←Older revision		Revision as of 10:28, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1h4d.~~png~~\|~~left~~\|200px]]	+	==BIOCHEMICAL AND STRUCTURAL ANALYSIS OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA==
		+	<StructureSection load='1h4d' size='340' side='right' caption='[[1h4d]], [[Resolution\|resolution]] 1.74Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H4D FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=LI:LITHIUM+ION'>LI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e5k\|1e5k]], [[1fr9\|1fr9]], [[1frw\|1frw]], [[1h4c\|1h4c]], [[1h4e\|1h4e]], [[1hjj\|1hjj]], [[1hjl\|1hjl]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h4d RCSB], [http://www.ebi.ac.uk/pdbsum/1h4d PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4d_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Molybdopterin guanine dinucleotide (MGD) is the form of the molybdenum cofactor that is required for the activity of most bacterial molybdoenzymes. MGD is synthesized from molybdopterin (MPT) and GTP in a reaction catalyzed by the MobA protein. Here we report that wild type MobA can be copurified along with bound MPT and MGD, demonstrating a tight binding of both its substrate and product. To study structure-function relationships, we have constructed a number of site-specific mutations of the most highly conserved amino acid residues of the MobA protein family. Variant MobA proteins were characterized for their ability to support the synthesis of active molybdenum enzymes, to bind MPT and MGD, to interact with the molybdenum cofactor biosynthesis proteins MobB and MoeA. They were also characterized by x-ray structural analysis. Our results suggest an essential role for glycine 15 of MobA, either for GTP binding and/or catalysis, and an involvement of glycine 82 in the stabilization of the product-bound form of the enzyme. Surprisingly, the individual and double substitution of asparagines 180 and 182 to aspartate did not affect MPT binding, catalysis, and product stabilization.

-	~~{{STRUCTURE_1h4d\| PDB=1h4d \| SCENE= }}~~	+	Biochemical and structural analysis of the molybdenum cofactor biosynthesis protein MobA.,Guse A, Stevenson CE, Kuper J, Buchanan G, Schwarz G, Giordano G, Magalon A, Mendel RR, Lawson DM, Palmer T J Biol Chem. 2003 Jul 11;278(28):25302-7. Epub 2003 Apr 28. PMID:12719427<ref>PMID:12719427</ref>

-	~~===BIOCHEMICAL AND STRUCTURAL ANALYSIS OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12719427}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1h4d]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA].~~	+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Buchanan, G.]]		[[Category: Buchanan, G.]]

OCA: Protected "1h4d" [edit=sysop:move=sysop]

2012-11-21T16:09:24Z

Protected "1h4d" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:09, 21 November 2012

OCA at 16:09, 21 November 2012

2012-11-21T16:09:21Z

←Older revision		Revision as of 16:09, 21 November 2012
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-	{{Seed}}
	[[Image:1h4d.png\|left\|200px]]		[[Image:1h4d.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1h4d", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1h4d\| PDB=1h4d \| SCENE= }}		{{STRUCTURE_1h4d\| PDB=1h4d \| SCENE= }}

	===BIOCHEMICAL AND STRUCTURAL ANALYSIS OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA===		===BIOCHEMICAL AND STRUCTURAL ANALYSIS OF THE MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN MOBA===

		+	{{ABSTRACT_PUBMED_12719427}}

	==About this Structure==		==About this Structure==
-	~~1H4D~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA].	+	[[1h4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4D OCA].
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Buchanan, G.]]		[[Category: Buchanan, G.]]
Line 27:		Line 21:
	[[Category: Molybdenum cofactor biosynthesis]]		[[Category: Molybdenum cofactor biosynthesis]]
	[[Category: Molybdopterin nucleotidyl-transferase]]		[[Category: Molybdopterin nucleotidyl-transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:40:43 2009''