1h9c - Revision history

OCA at 11:29, 27 March 2024

2024-03-27T11:29:40Z

←Older revision		Revision as of 11:29, 27 March 2024
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	==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==		==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==
-	<StructureSection load='1h9c' size='340' side='right'caption='[[1h9c~~]], [[NMR_Ensembles_of_Models \| 9 NMR models~~]]' scene=''>	+	<StructureSection load='1h9c' size='340' side='right'caption='[[1h9c]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecow3 Ecow3~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1H9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~316407 ECOW3])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [https://pdbe.org/1h9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [https://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [~~http~~://pdbe.org/1h9c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PTQB_ECOLI PTQB_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.<ref>PMID:10913117</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9c ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The determination by NMR of the solution structure of the phosphorylated enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli is presented. Most of the backbone and side-chain resonances were assigned using a variety of mostly heteronuclear NMR experiments. The remaining resonances were assigned with the help of the structure calculations.NOE-derived distance restraints were used in distance geometry calculations followed by molecular dynamics and simulated annealing protocols. In addition, combinations of ambiguous restraints were used to resolve ambiguities in the NOE assignments. By combining sets of ambiguous and unambiguous restraints into new ambiguous restraints, an error function was constructed that was less sensitive to information loss caused by assignment uncertainties. The final set of structures had a pairwise rmsd of 0.59 A and 1.16 A for the heavy atoms of the backbone and side-chains, respectively.Comparing the P-IIB(Chb) solution structure with the previously determined NMR and X-ray structures of the wild-type and the Cys10Ser mutant shows that significant differences between the structures are limited to the active-site region. The phosphoryl group at the active-site cysteine residue is surrounded by a loop formed by residues 10 through 16. NOE and chemical shift data suggest that the phosphoryl group makes hydrogen bonds with the backbone amide protons of residues 12 and 15. The binding mode of the phosphoryl group is very similar to that of the protein tyrosine phosphatases. The differences observed are in accordance with the presumption that IIB(Chb) has to be more resistant to hydrolysis than the protein tyrosine phosphatases. We propose a proton relay network by which a transfer occurs between the cysteine SH proton and the solvent via the hydroxyl group of Thr16.
-
-	NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.,Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM J Mol Biol. 2001 May 18;308(5):993-1009. PMID:11352587<ref>PMID:11352587</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1h9c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecow3~~]]	+	[[Category: Escherichia coli str. K-12 substr. W3110]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ab, E]]	+	[[Category: Ab E]]
-	[[Category: Dijkstra, K]]	+	[[Category: Dijkstra K]]
-	[[Category: Nijlant, D]]	+	[[Category: Nijlant D]]
-	[[Category: Robillard~~, G T~~]]	+	[[Category: Robillard GT]]
-	[[Category: Saier~~, M H~~]]	+	[[Category: Saier MH]]
-	[[Category: Scheek~~, R M~~]]	+	[[Category: Scheek RM]]
-	[[Category: Schuurman-Wolters~~, G K]]~~	+	[[Category: Schuurman-Wolters GK]]
-	~~[[Category: Enzyme iib-chitobiose]]~~	+
-	~~[[Category: Iib- cellobiose]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Phosphotransferase system]]~~	+
-	~~[[Category: Sugar transport]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:31, 18 November 2020

2020-11-18T10:31:20Z

←Older revision		Revision as of 10:31, 18 November 2020
Line 1:		Line 1:

	==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==		==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==
-	<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>	+	<StructureSection load='1h9c' size='340' side='right'caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1H9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 ECOW3])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 ECOW3])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://pdbe.org/1h9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://pdbe.org/1h9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Ecow3]]		[[Category: Ecow3]]
		+	[[Category: Large Structures]]
	[[Category: Ab, E]]		[[Category: Ab, E]]
	[[Category: Dijkstra, K]]		[[Category: Dijkstra, K]]

OCA at 08:54, 10 January 2018

2018-01-10T08:54:47Z

←Older revision		Revision as of 08:54, 10 January 2018
Line 1:		Line 1:
-	==NMR ~~STRUCTURE OF CYSTEINYL~~-~~PHOSPHORYLATED ENZYME~~ IIB ~~OF THE~~ N,N'-~~DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE~~-~~DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI~~.==	+
		+	==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==
	<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>		<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 ECOW3])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 ECOW3])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://pdbe.org/1h9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://pdbe.org/1h9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:01, 7 February 2016

2016-02-07T18:01:56Z

←Older revision		Revision as of 18:01, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:51, 10 September 2015

2015-09-10T03:51:44Z

←Older revision		Revision as of 03:51, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>		<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecow3 Ecow3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316407 ECOW3])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://pdbe.org/1h9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1h9c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecow3]]
	[[Category: Ab, E]]		[[Category: Ab, E]]
	[[Category: Dijkstra, K]]		[[Category: Dijkstra, K]]

OCA at 18:15, 22 December 2014

2014-12-22T18:15:38Z

←Older revision		Revision as of 18:15, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ab, E.]]	+	[[Category: Ab, E]]
-	[[Category: Dijkstra, K.]]	+	[[Category: Dijkstra, K]]
-	[[Category: Nijlant, D.]]	+	[[Category: Nijlant, D]]
-	[[Category: Robillard, G T.]]	+	[[Category: Robillard, G T]]
-	[[Category: Saier, M H.]]	+	[[Category: Saier, M H]]
-	[[Category: Scheek, R M.]]	+	[[Category: Scheek, R M]]
-	[[Category: Schuurman-Wolters, G K.]]	+	[[Category: Schuurman-Wolters, G K]]
	[[Category: Enzyme iib-chitobiose]]		[[Category: Enzyme iib-chitobiose]]
	[[Category: Iib- cellobiose]]		[[Category: Iib- cellobiose]]

OCA at 08:19, 28 September 2014

2014-09-28T08:19:47Z

←Older revision		Revision as of 08:19, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1h9c.~~png~~\|~~left~~\|200px]]	+	==NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.==
		+	<StructureSection load='1h9c' size='340' side='right' caption='[[1h9c]], [[NMR_Ensembles_of_Models \| 9 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHBB [PREV CELA] ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [http://www.ebi.ac.uk/pdbsum/1h9c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The determination by NMR of the solution structure of the phosphorylated enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli is presented. Most of the backbone and side-chain resonances were assigned using a variety of mostly heteronuclear NMR experiments. The remaining resonances were assigned with the help of the structure calculations.NOE-derived distance restraints were used in distance geometry calculations followed by molecular dynamics and simulated annealing protocols. In addition, combinations of ambiguous restraints were used to resolve ambiguities in the NOE assignments. By combining sets of ambiguous and unambiguous restraints into new ambiguous restraints, an error function was constructed that was less sensitive to information loss caused by assignment uncertainties. The final set of structures had a pairwise rmsd of 0.59 A and 1.16 A for the heavy atoms of the backbone and side-chains, respectively.Comparing the P-IIB(Chb) solution structure with the previously determined NMR and X-ray structures of the wild-type and the Cys10Ser mutant shows that significant differences between the structures are limited to the active-site region. The phosphoryl group at the active-site cysteine residue is surrounded by a loop formed by residues 10 through 16. NOE and chemical shift data suggest that the phosphoryl group makes hydrogen bonds with the backbone amide protons of residues 12 and 15. The binding mode of the phosphoryl group is very similar to that of the protein tyrosine phosphatases. The differences observed are in accordance with the presumption that IIB(Chb) has to be more resistant to hydrolysis than the protein tyrosine phosphatases. We propose a proton relay network by which a transfer occurs between the cysteine SH proton and the solvent via the hydroxyl group of Thr16.

-	~~{{STRUCTURE_1h9c\| PDB=1h9c \| SCENE= }}~~	+	NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.,Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM J Mol Biol. 2001 May 18;308(5):993-1009. PMID:11352587<ref>PMID:11352587</ref>

-	~~===NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N~~,~~N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11352587}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1h9c]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphotransferase\|Phosphotransferase]]		*[[Phosphotransferase\|Phosphotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011352587<~~/~~ref~~><~~ref group="xtra">PMID:009041631</ref><ref group="xtra">PMID:008003964</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ab, E.]]		[[Category: Ab, E.]]

OCA at 07:27, 20 October 2012

2012-10-20T07:27:42Z

←Older revision		Revision as of 07:27, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1h9c]] is a 1 chain structure ~~of [[Phosphotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA].	+	[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:011352587</ref><ref group="xtra">PMID:009041631</ref><ref group="xtra">PMID:008003964~~</ref><ref group="xtra">PMID:002179047</ref><ref group="xtra">PMID:002092358~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011352587</ref><ref group="xtra">PMID:009041631</ref><ref group="xtra">PMID:008003964</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ab, E.]]		[[Category: Ab, E.]]

OCA at 16:23, 25 July 2012

2012-07-25T16:23:51Z

←Older revision		Revision as of 16:23, 25 July 2012
Line 1:		Line 1:
	[[Image:1h9c.png\|left\|200px]]		[[Image:1h9c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1h9c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1h9c\| PDB=1h9c \| SCENE= }}		{{STRUCTURE_1h9c\| PDB=1h9c \| SCENE= }}

	===NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.===		===NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11352587}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11352587 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11352587}}		{{ABSTRACT_PUBMED_11352587}}

	==About this Structure==		==About this Structure==
-	[[1h9c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA].	+	[[1h9c]] is a 1 chain structure of [[Phosphotransferase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA].
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11352587~~</ref><ref group="xtra">PMID:~~9041631~~</ref><ref group="xtra">PMID:~~8003964~~</ref><ref group="xtra">PMID:~~2179047~~</ref><ref group="xtra">PMID:~~2092358~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011352587</ref><ref group="xtra">PMID:009041631</ref><ref group="xtra">PMID:008003964</ref><ref group="xtra">PMID:002179047</ref><ref group="xtra">PMID:002092358</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ab, E.]]		[[Category: Ab, E.]]

OCA: Protected "1h9c" [edit=sysop:move=sysop]

2011-03-14T12:44:03Z

Protected "1h9c" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:44, 14 March 2011