1hjq - Revision history

OCA at 11:32, 27 March 2024

2024-03-27T11:32:34Z

←Older revision		Revision as of 11:32, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1hjq' size='340' side='right'caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1hjq' size='340' side='right'caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_16454 Atcc 16454~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1hjs\|1hjs]], [[1hju\|1hju]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Arabinogalactan_endo~~-~~beta~~-~~1,4~~-~~galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [https://pdbe.org/1hjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [https://www.ebi.ac.uk/pdbsum/1hjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [https://pdbe.org/1hjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [https://www.ebi.ac.uk/pdbsum/1hjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GANA_HUMIN GANA_HUMIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	beta-1,4-Galactanases hydrolyze the galactan side chains that are part of the complex carbohydrate structure of the pectin. They are assigned to family 53 of the glycoside hydrolases and display significant variations in their pH and temperature optimum and stability. Two fungal beta-1,4-galactanases from Myceliophthora thermophila and Humicola insolens have been cloned and heterologously expressed, and the crystal structures of the gene products were determined. The structures are compared to the previously only known family 53 structure of the galactanase from Aspergillus aculeatus (AAGAL) showing approximately 56% identity. The M. thermophila and H. insolens galactanases are thermophilic enzymes and are most active at neutral to basic pH, whereas AAGAL is mesophilic and most active at acidic pH. The structure of the M. thermophila galactanase (MTGAL) was determined from crystals obtained with HEPES and TRIS buffers to 1.88 A and 2.14 A resolution, respectively. The structure of the H. insolens galactanase (HIGAL) was determined to 2.55 A resolution. The thermostability of MTGAL and HIGAL correlates with increase in the protein rigidity and electrostatic interactions, stabilization of the alpha-helices, and a tighter packing. An inspection of the active sites in the three enzymes identifies several amino acid substitutions that could explain the variation in pH optimum. Examination of the activity as a function of pH for the D182N mutant of AAGAL and the A90S/ H91D mutant of MTGAL showed that the difference in pH optimum between AAGAL and MTGAL is at least partially associated with differences in the nature of residues at positions 182, 90, and/or 91.
-
-	Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.,Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S Protein Sci. 2003 Jun;12(6):1195-204. PMID:12761390<ref>PMID:12761390</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]		*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabinogalactan endo-beta-1,4-galactanase]]~~	+	[[Category: Humicola insolens]]
-	~~[[Category: Atcc 16454~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Borchert~~, T V~~]]	+	[[Category: Borchert TV]]
-	[[Category: Christensen~~, L L.H~~]]	+	[[Category: Christensen LLH]]
-	[[Category: Larsen, S]]	+	[[Category: Larsen S]]
-	[[Category: ~~Leggio, L Lo~~]]	+	[[Category: Le Nours J]]
-	[[Category: ~~Nours, J Le~~]]	+	[[Category: Lo Leggio L]]
-	[[Category: Ostergaard~~, P R~~]]	+	[[Category: Ostergaard PR]]
-	[[Category: Ryttersgaard, C]]	+	[[Category: Ryttersgaard C]]
-	~~[[Category: 4-galactanase]]~~	+
-	~~[[Category: Alkalophile]]~~	+
-	~~[[Category: Beta-1]]~~	+
-	~~[[Category: Clan gh-a]]~~	+
-	~~[[Category: Family 53 glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ph optimum]]~~	+
-	~~[[Category: Thermophile]]~~	+
-	~~[[Category: Thermostability~~]]	+

OCA at 10:50, 4 August 2021

2021-08-04T10:50:00Z

←Older revision		Revision as of 10:50, 4 August 2021
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	<StructureSection load='1hjq' size='340' side='right'caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1hjq' size='340' side='right'caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hjs\|1hjs]], [[1hju\|1hju]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [~~http~~://pdbe.org/1hjq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1hjq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [https://pdbe.org/1hjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [https://www.ebi.ac.uk/pdbsum/1hjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:22, 30 October 2019

2019-10-30T10:22:35Z

←Older revision		Revision as of 10:22, 30 October 2019
Line 1:		Line 1:

	==Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.==		==Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.==
-	<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='1hjq' size='340' side='right'caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	[[Category: Arabinogalactan endo-beta-1,4-galactanase]]		[[Category: Arabinogalactan endo-beta-1,4-galactanase]]
	[[Category: Atcc 16454]]		[[Category: Atcc 16454]]
		+	[[Category: Large Structures]]
	[[Category: Borchert, T V]]		[[Category: Borchert, T V]]
	[[Category: Christensen, L L.H]]		[[Category: Christensen, L L.H]]

OCA at 09:23, 10 January 2018

2018-01-10T09:23:33Z

←Older revision		Revision as of 09:23, 10 January 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF TWO FUNGAL BETA~~-1,4-~~GALACTANASES~~: ~~SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM~~.==	+
		+	==Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.==
	<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://pdbe.org/1hjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://pdbe.org/1hjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjq ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:34, 8 February 2016

2016-02-08T20:34:12Z

←Older revision		Revision as of 20:34, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:10, 10 September 2015

2015-09-10T15:10:05Z

←Older revision		Revision as of 15:10, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Humicola_insolens Humicola insolens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-~~beta-galactosidase~~ Arabinogalactan endo-1,4-~~beta-galactosidase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-beta-1,4-galactanase Arabinogalactan endo-beta-1,4-galactanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://pdbe.org/1hjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1hjq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arabinogalactan endo-1,4-~~beta-galactosidase~~]]	+	[[Category: Arabinogalactan endo-beta-1,4-galactanase]]
-	[[Category: ~~Humicola insolens~~]]	+	[[Category: Atcc 16454]]
	[[Category: Borchert, T V]]		[[Category: Borchert, T V]]
	[[Category: Christensen, L L.H]]		[[Category: Christensen, L L.H]]

OCA at 23:49, 22 December 2014

2014-12-22T23:49:53Z

←Older revision		Revision as of 23:49, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]		[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]
-	[[Category: Borchert, T V.]]	+	[[Category: Borchert, T V]]
-	[[Category: Christensen, L L.H.]]	+	[[Category: Christensen, L L.H]]
-	[[Category: Larsen, S.]]	+	[[Category: Larsen, S]]
-	[[Category: Leggio, L Lo.]]	+	[[Category: Leggio, L Lo]]
-	[[Category: Nours, J Le.]]	+	[[Category: Nours, J Le]]
-	[[Category: Ostergaard, P R.]]	+	[[Category: Ostergaard, P R]]
-	[[Category: Ryttersgaard, C.]]	+	[[Category: Ryttersgaard, C]]
	[[Category: 4-galactanase]]		[[Category: 4-galactanase]]
	[[Category: Alkalophile]]		[[Category: Alkalophile]]

OCA at 13:34, 28 September 2014

2014-09-28T13:34:27Z

←Older revision		Revision as of 13:34, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1hjq.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF TWO FUNGAL BETA-1,4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.==
		+	<StructureSection load='1hjq' size='340' side='right' caption='[[1hjq]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HJQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hjs\|1hjs]], [[1hju\|1hju]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinogalactan_endo-1,4-beta-galactosidase Arabinogalactan endo-1,4-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.89 3.2.1.89] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hjq RCSB], [http://www.ebi.ac.uk/pdbsum/1hjq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-1,4-Galactanases hydrolyze the galactan side chains that are part of the complex carbohydrate structure of the pectin. They are assigned to family 53 of the glycoside hydrolases and display significant variations in their pH and temperature optimum and stability. Two fungal beta-1,4-galactanases from Myceliophthora thermophila and Humicola insolens have been cloned and heterologously expressed, and the crystal structures of the gene products were determined. The structures are compared to the previously only known family 53 structure of the galactanase from Aspergillus aculeatus (AAGAL) showing approximately 56% identity. The M. thermophila and H. insolens galactanases are thermophilic enzymes and are most active at neutral to basic pH, whereas AAGAL is mesophilic and most active at acidic pH. The structure of the M. thermophila galactanase (MTGAL) was determined from crystals obtained with HEPES and TRIS buffers to 1.88 A and 2.14 A resolution, respectively. The structure of the H. insolens galactanase (HIGAL) was determined to 2.55 A resolution. The thermostability of MTGAL and HIGAL correlates with increase in the protein rigidity and electrostatic interactions, stabilization of the alpha-helices, and a tighter packing. An inspection of the active sites in the three enzymes identifies several amino acid substitutions that could explain the variation in pH optimum. Examination of the activity as a function of pH for the D182N mutant of AAGAL and the A90S/ H91D mutant of MTGAL showed that the difference in pH optimum between AAGAL and MTGAL is at least partially associated with differences in the nature of residues at positions 182, 90, and/or 91.

-	~~{{STRUCTURE_1hjq\| PDB=1hjq \| SCENE= }}~~	+	Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.,Le Nours J, Ryttersgaard C, Lo Leggio L, Ostergaard PR, Borchert TV, Christensen LL, Larsen S Protein Sci. 2003 Jun;12(6):1195-204. PMID:12761390<ref>PMID:12761390</ref>

-	~~===STRUCTURE OF TWO FUNGAL BETA-1~~,~~4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12761390}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1hjq]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Humicola_insolens Humicola insolens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]		*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012761390<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]		[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]

OCA: Protected "1hjq" [edit=sysop:move=sysop]

2012-11-21T16:07:37Z

Protected "1hjq" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:07, 21 November 2012

OCA at 16:07, 21 November 2012

2012-11-21T16:07:31Z

←Older revision		Revision as of 16:07, 21 November 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1hjq.png\|left\|200px]]		[[Image:1hjq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1hjq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1hjq\| PDB=1hjq \| SCENE= }}		{{STRUCTURE_1hjq\| PDB=1hjq \| SCENE= }}

	===STRUCTURE OF TWO FUNGAL BETA-1,4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.===		===STRUCTURE OF TWO FUNGAL BETA-1,4-GALACTANASES: SEARCHING FOR THE BASIS FOR TEMPERATURE AND PH OPTIMUM.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12761390}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12761390 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12761390}}		{{ABSTRACT_PUBMED_12761390}}

	==About this Structure==		==About this Structure==
-	~~1HJQ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA].	+	[[1hjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJQ OCA].
		+
		+	==See Also==
		+	*[[Beta-1%2C4-galactanase\|Beta-1%2C4-galactanase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12761390~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012761390</ref><references group="xtra"/>
	[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]		[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
	[[Category: Humicola insolens]]		[[Category: Humicola insolens]]
Line 38:		Line 29:
	[[Category: Clan gh-a]]		[[Category: Clan gh-a]]
	[[Category: Family 53 glycoside hydrolase]]		[[Category: Family 53 glycoside hydrolase]]
		+	[[Category: Hydrolase]]
	[[Category: Ph optimum]]		[[Category: Ph optimum]]
	[[Category: Thermophile]]		[[Category: Thermophile]]
	[[Category: Thermostability]]		[[Category: Thermostability]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 02:54:23 2009''