1hwn - Revision history

OCA at 11:35, 27 March 2024

2024-03-27T11:35:44Z

←Older revision		Revision as of 11:35, 27 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GAL:BETA~~-~~D-GALACTOSE'>GAL</scene>~~, ~~<scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1hwm\|1hwm]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA~~ N-~~glycosylase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [https://pdbe.org/1hwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [https://www.ebi.ac.uk/pdbsum/1hwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [https://pdbe.org/1hwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [https://www.ebi.ac.uk/pdbsum/1hwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9AVR2_SAMEB Q9AVR2_SAMEB]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hwn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hwn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Ebulin l is a type-II ribosome-inactivating protein (RIP) isolated from the leaves of Sambucus ebulus L. As with other type-II RIP, ebulin is a disulfide-linked heterodimer composed of a toxic A chain and a galactoside-specific lectin B chain. A normal level of ribosome-inactivating N-glycosidase activity, characteristic of the A chain of type-II RIP, has been demonstrated for ebulin l. However, ebulin is considered a nontoxic type-II RIP due to a reduced cytotoxicity on whole cells and animals as compared with other toxic type-II RIP like ricin. The molecular cloning, amino acid sequence, and the crystal structure of ebulin l are presented and compared with ricin. Ebulin l is shown to bind an A-chain substrate analogue, pteroic acid, in the same manner as ricin. The galactoside-binding ability of ebulin l is demonstrated crystallographically with a complex of the B chain with galactose and with lactose. The negligible cytotoxicity of ebulin l is apparently due to a reduced affinity for galactosides. An altered mode of galactoside binding in the 2gamma subdomain of the lectin B chain primarily causes the reduced affinity.
-
-	2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l.,Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T Proteins. 2001 May 15;43(3):319-26. PMID:11288182<ref>PMID:11288182</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1hwn" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sambucus ebulus]]		[[Category: Sambucus ebulus]]
-	~~[[Category: RRNA N-glycosylase]]~~	+	[[Category: Day PJ]]
-	[[Category: Day~~, P J~~]]	+	[[Category: Ernst SR]]
-	[[Category: Ernst~~, S R~~]]	+	[[Category: Monzingo AF]]
-	[[Category: Monzingo~~, A F~~]]	+	[[Category: Pascal JM]]
-	[[Category: Pascal~~, J M~~]]	+	[[Category: Robertus JD]]
-	[[Category: Robertus~~, J D]]~~	+
-	~~[[Category: Galactoside lectin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ribosome-inactivating protein]]~~	+
-	~~[[Category: Ricin-like~~]]	+

OCA at 07:29, 7 April 2021

2021-04-07T07:29:56Z

←Older revision		Revision as of 07:29, 7 April 2021
Line 3:		Line 3:
	<StructureSection load='1hwn' size='340' side='right'caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1hwn' size='340' side='right'caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HWN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hwm\|1hwm]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [~~http~~://pdbe.org/1hwn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1hwn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [https://pdbe.org/1hwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [https://www.ebi.ac.uk/pdbsum/1hwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:30, 3 July 2019

2019-07-03T07:30:06Z

←Older revision		Revision as of 07:30, 3 July 2019
Line 1:		Line 1:

	==EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM==		==EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM==
-	<StructureSection load='1hwn' size='340' side='right' caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1hwn' size='340' side='right'caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HWN FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/1hwn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/1hwn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sambucus ebulus]]		[[Category: Sambucus ebulus]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]

OCA at 10:53, 27 September 2017

2017-09-27T10:53:21Z

←Older revision		Revision as of 10:53, 27 September 2017
Line 1:		Line 1:
		+
	==EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM==		==EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM==
	<StructureSection load='1hwn' size='340' side='right' caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1hwn' size='340' side='right' caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://pdbe.org/1hwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://pdbe.org/1hwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hwn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hwn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:37, 11 September 2015

2015-09-11T17:37:13Z

←Older revision		Revision as of 17:37, 11 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://pdbe.org/1hwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1hwn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:51, 2 January 2015

2015-01-02T09:51:02Z

←Older revision		Revision as of 09:51, 2 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HWN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Sambucus ebulus]]		[[Category: Sambucus ebulus]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
-	[[Category: Day, P J.]]	+	[[Category: Day, P J]]
-	[[Category: Ernst, S R.]]	+	[[Category: Ernst, S R]]
-	[[Category: Monzingo, A F.]]	+	[[Category: Monzingo, A F]]
-	[[Category: Pascal, J M.]]	+	[[Category: Pascal, J M]]
-	[[Category: Robertus, J D.]]	+	[[Category: Robertus, J D]]
	[[Category: Galactoside lectin]]		[[Category: Galactoside lectin]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Ribosome-inactivating protein]]		[[Category: Ribosome-inactivating protein]]
	[[Category: Ricin-like]]		[[Category: Ricin-like]]

OCA at 09:24, 28 September 2014

2014-09-28T09:24:04Z

←Older revision		Revision as of 09:24, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1hwn.~~png~~\|~~left~~\|200px]]	+	==EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM==
		+	<StructureSection load='1hwn' size='340' side='right' caption='[[1hwn]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HWN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hwm\|1hwm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hwn RCSB], [http://www.ebi.ac.uk/pdbsum/1hwn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/1hwn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ebulin l is a type-II ribosome-inactivating protein (RIP) isolated from the leaves of Sambucus ebulus L. As with other type-II RIP, ebulin is a disulfide-linked heterodimer composed of a toxic A chain and a galactoside-specific lectin B chain. A normal level of ribosome-inactivating N-glycosidase activity, characteristic of the A chain of type-II RIP, has been demonstrated for ebulin l. However, ebulin is considered a nontoxic type-II RIP due to a reduced cytotoxicity on whole cells and animals as compared with other toxic type-II RIP like ricin. The molecular cloning, amino acid sequence, and the crystal structure of ebulin l are presented and compared with ricin. Ebulin l is shown to bind an A-chain substrate analogue, pteroic acid, in the same manner as ricin. The galactoside-binding ability of ebulin l is demonstrated crystallographically with a complex of the B chain with galactose and with lactose. The negligible cytotoxicity of ebulin l is apparently due to a reduced affinity for galactosides. An altered mode of galactoside binding in the 2gamma subdomain of the lectin B chain primarily causes the reduced affinity.

-	~~{{STRUCTURE_1hwn\| PDB=1hwn \| SCENE= }}~~	+	2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l.,Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T Proteins. 2001 May 15;43(3):319-26. PMID:11288182<ref>PMID:11288182</ref>

-	~~===EBULIN COMPLEXED WITH GALACTOSE~~, ~~TRIGONAL CRYSTAL FORM===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11288182}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1hwn]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sambucus_ebulus Sambucus ebulus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011288182<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sambucus ebulus]]		[[Category: Sambucus ebulus]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]

OCA: Protected "1hwn" [edit=sysop:move=sysop]

2012-11-21T15:52:46Z

Protected "1hwn" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:52, 21 November 2012

OCA at 15:52, 21 November 2012

2012-11-21T15:52:44Z

←Older revision		Revision as of 15:52, 21 November 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1hwn.png\|left\|200px]]		[[Image:1hwn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1hwn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1hwn\| PDB=1hwn \| SCENE= }}		{{STRUCTURE_1hwn\| PDB=1hwn \| SCENE= }}

	===EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM===		===EBULIN COMPLEXED WITH GALACTOSE, TRIGONAL CRYSTAL FORM===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11288182}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11288182 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11288182}}		{{ABSTRACT_PUBMED_11288182}}

	==About this Structure==		==About this Structure==
-	~~1HWN~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA].	+	[[1hwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11288182~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011288182</ref><references group="xtra"/>
	[[Category: Sambucus ebulus]]		[[Category: Sambucus ebulus]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
Line 32:		Line 20:
	[[Category: Robertus, J D.]]		[[Category: Robertus, J D.]]
	[[Category: Galactoside lectin]]		[[Category: Galactoside lectin]]
		+	[[Category: Hydrolase]]
	[[Category: Ribosome-inactivating protein]]		[[Category: Ribosome-inactivating protein]]
	[[Category: Ricin-like]]		[[Category: Ricin-like]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:53:28 2009''

OCA at 02:53, 18 February 2009

2009-02-18T02:53:29Z

←Older revision		Revision as of 02:53, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA].	+	1HWN is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sambucus_ebulus Sambucus ebulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWN OCA].

	==Reference==		==Reference==
-	2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/11288182 11288182~~]	+	<ref group="xtra">PMID:11288182</ref><references group="xtra"/>
		+	[[Category: Sambucus ebulus]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Day, P J.]]		[[Category: Day, P J.]]
Line 34:		Line 35:
	[[Category: Ricin-like]]		[[Category: Ricin-like]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1 10~~:01:~~34 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:53:28 2009''