1ii2 - Revision history

OCA at 08:33, 16 August 2023

2023-08-16T08:33:53Z

←Older revision		Revision as of 08:33, 16 August 2023
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	<StructureSection load='1ii2' size='340' side='right'caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ii2' size='340' side='right'caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1II2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>[https://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [https://pdbe.org/1ii2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ii2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [https://pdbe.org/1ii2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ii2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PCKA_TRYCR PCKA_TRYCR]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Craievich~~, A F~~]]	+	[[Category: Trypanosoma cruzi]]
-	[[Category: Fischer, H]]	+	[[Category: Craievich AF]]
-	[[Category: Goldenberg, S]]	+	[[Category: Fischer H]]
-	[[Category: Linss, J]]	+	[[Category: Goldenberg S]]
-	[[Category: Oliva, G]]	+	[[Category: Linss J]]
-	[[Category: Trapani, S]]	+	[[Category: Oliva G]]
-	~~[[Category: Lyase]]~~	+	[[Category: Trapani S]]
-	~~[[Category: Phosphate binding loop~~]]	+

OCA at 07:43, 7 April 2021

2021-04-07T07:43:15Z

←Older revision		Revision as of 07:43, 7 April 2021
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	<StructureSection load='1ii2' size='340' side='right'caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ii2' size='340' side='right'caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1II2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [~~http~~://pdbe.org/1ii2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ii2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [https://pdbe.org/1ii2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ii2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Phosphoenolpyruvate carboxykinase\|Phosphoenolpyruvate carboxykinase]]	+	*[[Phosphoenolpyruvate carboxykinase 3D structures\|Phosphoenolpyruvate carboxykinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:37, 3 July 2019

2019-07-03T07:37:46Z

←Older revision		Revision as of 07:37, 3 July 2019
Line 1:		Line 1:

	==Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi==		==Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi==
-	<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1ii2' size='340' side='right'caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1ii2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1ii2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Craievich, A F]]		[[Category: Craievich, A F]]
	[[Category: Fischer, H]]		[[Category: Fischer, H]]

OCA at 10:40, 27 September 2017

2017-09-27T10:40:18Z

←Older revision		Revision as of 10:40, 27 September 2017
Line 1:		Line 1:
		+
	==Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi==		==Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi==
	<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://pdbe.org/1ii2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://pdbe.org/1ii2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ii2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Trycr]]
	[[Category: Craievich, A F]]		[[Category: Craievich, A F]]
	[[Category: Fischer, H]]		[[Category: Fischer, H]]

OCA at 16:55, 11 September 2015

2015-09-11T16:55:15Z

←Older revision		Revision as of 16:55, 11 September 2015
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	<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Trypanosoma_cruzi Trypanosoma cruzi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://pdbe.org/1ii2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ii2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Trypanosoma cruzi~~]]	+	[[Category: Trycr]]
	[[Category: Craievich, A F]]		[[Category: Craievich, A F]]
	[[Category: Fischer, H]]		[[Category: Fischer, H]]

OCA at 12:04, 2 January 2015

2015-01-02T12:04:34Z

←Older revision		Revision as of 12:04, 2 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]
-	[[Category: Craievich, A F.]]	+	[[Category: Craievich, A F]]
-	[[Category: Fischer, H.]]	+	[[Category: Fischer, H]]
-	[[Category: Goldenberg, S.]]	+	[[Category: Goldenberg, S]]
-	[[Category: Linss, J.]]	+	[[Category: Linss, J]]
-	[[Category: Oliva, G.]]	+	[[Category: Oliva, G]]
-	[[Category: Trapani, S.]]	+	[[Category: Trapani, S]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Phosphate binding loop]]		[[Category: Phosphate binding loop]]

OCA at 08:34, 3 October 2014

2014-10-03T08:34:48Z

←Older revision		Revision as of 08:34, 3 October 2014
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-	~~{{STRUCTURE_1ii2\| PDB=1ii2 \| SCENE= }}~~	+	==Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi==
-	===Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi===	+	<StructureSection load='1ii2' size='340' side='right' caption='[[1ii2]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_11700062}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1II2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ii2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ii2 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1ii2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ATP-dependent phosphoenolpyruvate carboxykinase (PEPCK) (ATP: oxaloacetate carboxylyase (transphosphorylating), EC 4.1.1.49) is a key enzyme involved in the catabolism of glucose and amino acids in the parasite Trypanosoma cruzi, the causative agent of Chagas' disease. Due to the significant differences in the amino acid sequence and substrate specificity of the human enzyme (PEPCK (GTP-dependent), EC 4.1.1.32), the parasite enzyme has been considered a good target for the development of new anti-chagasic drugs. We have solved the crystal structure of the recombinant PEPCK of T. cruzi up to 2.0 A resolution, characterised the dimeric organisation of the enzyme by solution small angle X-ray scattering (SAXS) and compared the enzyme structure with the known crystal structure of the monomeric PEPCK from Escherichia coli. The dimeric structure possesses 2-fold symmetry, with each monomer sharing a high degree of structural similarity with the monomeric structure of the E. coli PEPCK. Each monomer folds into two complex mixed alpha/beta domains, with the active site located in a deep cleft between the domains. The two active sites in the dimer are far apart from each other, in an arrangement that seems to permit an independent access of the substrates to the two active sites. All residues of the E. coli PEPCK structure that had been found to interact with substrates and metal cofactors have been found conserved and in a substantially equivalent spatial disposition in the T. cruzi PEPCK structure. No substrate or metal ion was present in the crystal structure. A sulphate ion from the crystallisation medium has been found bound to the active site. Solution SAXS data suggest that, in solutions with lower sulphate concentration than that used for the crystallisation experiments, the actual enzyme conformation may be slightly different from its conformation in the crystal structure. This could be due to a conformational transition upon sulphate binding, similar to the ATP-induced transition observed in the E. coli PEPCK, or to crystal packing effects. The present structure of the T. cruzi PEPCK will provide a good basis for the modelling of new anti-chagasic drug leads.

-	~~==About this Structure==~~	+	Crystal structure of the dimeric phosphoenolpyruvate carboxykinase (PEPCK) from Trypanosoma cruzi at 2 A resolution.,Trapani S, Linss J, Goldenberg S, Fischer H, Craievich AF, Oliva G J Mol Biol. 2001 Nov 9;313(5):1059-72. PMID:11700062<ref>PMID:11700062</ref>
-	~~[[1ii2]] is a 2 chain~~ structure ~~with sequence~~ from ~~[http://en.wikipedia.org/wiki/Trypanosoma_cruzi~~ Trypanosoma cruzi]. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1II2 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Phosphoenolpyruvate carboxykinase\|Phosphoenolpyruvate carboxykinase]]		*[[Phosphoenolpyruvate carboxykinase\|Phosphoenolpyruvate carboxykinase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:011700062</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]
	[[Category: Craievich, A F.]]		[[Category: Craievich, A F.]]

OCA at 10:35, 16 April 2014

2014-04-16T10:35:23Z

←Older revision		Revision as of 10:35, 16 April 2014
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-	[[Image:1ii2.png\|left\|200px]]
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-	<!--
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	{{STRUCTURE_1ii2\| PDB=1ii2 \| SCENE= }}		{{STRUCTURE_1ii2\| PDB=1ii2 \| SCENE= }}
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	===Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi===		===Crystal Structure of Phosphoenolpyruvate Carboxykinase (PEPCK) from Trypanosoma cruzi===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11700062}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_11700062}}		{{ABSTRACT_PUBMED_11700062}}

	==About this Structure==		==About this Structure==
	[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA].		[[1ii2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II2 OCA].
		+
		+	==See Also==
		+	*[[Phosphoenolpyruvate carboxykinase\|Phosphoenolpyruvate carboxykinase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:011700062</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011700062</ref><references group="xtra"/><references/>
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]
	[[Category: Craievich, A F.]]		[[Category: Craievich, A F.]]

OCA: Protected "1ii2" [edit=sysop:move=sysop]

2012-06-02T09:03:51Z

Protected "1ii2" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:03, 2 June 2012