1in9 - Revision history

OCA at 11:02, 4 August 2021

2021-08-04T11:02:22Z

←Older revision		Revision as of 11:02, 4 August 2021
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	<StructureSection load='1in9' size='340' side='right'caption='[[1in9]]' scene=''>		<StructureSection load='1in9' size='340' side='right'caption='[[1in9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1in9 FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/1in9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1in9 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1in9 FirstGlance], [https://www.ebi.ac.uk/pdbsum/1in9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1in9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:19, 30 October 2019

2019-10-30T10:19:55Z

←Older revision		Revision as of 10:19, 30 October 2019
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	==HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS==		==HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS==
-	<StructureSection load='1in9' size='340' side='right' caption='[[1in9]]' scene=''>	+	<StructureSection load='1in9' size='340' side='right'caption='[[1in9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Jiang, G C.T]]		[[Category: Jiang, G C.T]]
	[[Category: Schmitt, J D]]		[[Category: Schmitt, J D]]
	[[Category: Vrana, K E]]		[[Category: Vrana, K E]]
	[[Category: Yohrling Iv, G J]]		[[Category: Yohrling Iv, G J]]

OCA at 09:21, 10 January 2018

2018-01-10T09:21:32Z

←Older revision		Revision as of 09:21, 10 January 2018
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in9 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1in9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in9 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1in9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1in9 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:04, 11 February 2016

2016-02-11T09:04:06Z

←Older revision		Revision as of 09:04, 11 February 2016
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		+	{{Theoretical_model}}
		+
	==HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS==		==HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS==
	<StructureSection load='1in9' size='340' side='right' caption='[[1in9]]' scene=''>		<StructureSection load='1in9' size='340' side='right' caption='[[1in9]]' scene=''>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Jiang, G C.T]]		[[Category: Jiang, G C.T]]
	[[Category: Schmitt, J D]]		[[Category: Schmitt, J D]]
	[[Category: Vrana, K E]]		[[Category: Vrana, K E]]
	[[Category: Yohrling Iv, G J]]		[[Category: Yohrling Iv, G J]]

OCA at 06:21, 11 September 2015

2015-09-11T06:21:16Z

←Older revision		Revision as of 06:21, 11 September 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1in9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:33, 2 January 2015

2015-01-02T09:33:54Z

←Older revision		Revision as of 09:33, 2 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in9 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1in9 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in9 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1in9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:25, 28 September 2014

2014-09-28T10:25:47Z

←Older revision		Revision as of 10:25, 28 September 2014
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-	~~{{Theoretical_model}}~~	+	==HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS==
		+	<StructureSection load='1in9' size='340' side='right' caption='[[1in9]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IN9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1in9 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1in9 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in the biosynthesis of serotonin. The inherent instability of TPH has prevented a crystallographic structure from being resolved. For this reason, multiple sequence alignment-based molecular modeling was utilized to generate a full-length model of human TPH. Previously determined crystal coordinates of two highly homologous proteins, phenylalanine hydroxylase and tyrosine hydroxylase, were used as templates. Analysis of the model aided rational mutagenesis studies to further dissect the regulation and catalysis of TPH. Using rational site-directed mutagenesis, it was determined that Tyr235 (Y235), within the active site of TPH, appears to be involved as a tryptophan substrate orienting residue. The mutants Y235A and Y235L displayed reduced specific activity compared to wild-type TPH ( approximately 5 % residual activity). The K(m) of tryptophan for the Y235A (564 microM) and Y235L (96 microM) mutant was significantly increased compared to wild-type TPH (42 microM). In addition, kinetic analyses were performed on wild-type TPH and a deletion construct that lacks the amino terminal autoregulatory sequence (TPH NDelta15). This sequence in phenylalanine hydroxylase (residues 19 to 33) has previously been proposed to act as a steric regulator of substrate accessibility to the active site. Changes in the steady-state kinetics for tetrahydrobiopterin (BH(4)) and tryptophan for TPH NDelta15 were not observed. Finally, it was demonstrated that both Ser58 and Ser260 are substrates for Ca(2+)/calmodulin-dependent protein kinase II. Additional analysis of this model will aid in deciphering the regulation and substrate specificity of TPH, as well as providing a basis to understand as yet to be identified polymorphisms.

-	~~[[Image~~:~~1in9~~.~~png\|left\|200px]]~~	+	Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling.,Jiang GC, Yohrling GJ 4th, Schmitt JD, Vrana KE J Mol Biol. 2000 Sep 29;302(4):1005-17. PMID:10993738<ref>PMID:10993738</ref>

-	~~{{STRUCTURE_1in9\| PDB=1in9 \| SCENE= }}~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~===HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY~~, ~~CATALYTIC AND TETRAMERIZATION DOMAINS===~~	+	== References ==
-		+	<references/>
-	~~{{ABSTRACT_PUBMED_10993738}}~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010993738<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Jiang, G C.T]]		[[Category: Jiang, G C.T]]
	[[Category: Schmitt, J D]]		[[Category: Schmitt, J D]]
	[[Category: Vrana, K E]]		[[Category: Vrana, K E]]
	[[Category: Yohrling Iv, G J]]		[[Category: Yohrling Iv, G J]]

OCA: Protected "1in9" [edit=sysop:move=sysop]

2012-10-21T09:08:13Z

Protected "1in9" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:08, 21 October 2012

OCA at 09:08, 21 October 2012

2012-10-21T09:08:10Z

←Older revision		Revision as of 09:08, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:1in9.png\|left\|200px]]		[[Image:1in9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1in9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1in9\| PDB=1in9 \| SCENE= }}		{{STRUCTURE_1in9\| PDB=1in9 \| SCENE= }}

	===HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS===		===HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10993738}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10993738 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10993738}}		{{ABSTRACT_PUBMED_10993738}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10993738~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010993738</ref><references group="xtra"/>
	[[Category: Jiang, G C.T]]		[[Category: Jiang, G C.T]]
	[[Category: Schmitt, J D]]		[[Category: Schmitt, J D]]
	[[Category: Vrana, K E]]		[[Category: Vrana, K E]]
	[[Category: Yohrling Iv, G J]]		[[Category: Yohrling Iv, G J]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:44:27 2010''

OCA at 04:44, 8 April 2010

2010-04-08T04:44:28Z

←Older revision		Revision as of 04:44, 8 April 2010
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-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:1in9.png\|left\|200px]]

-	The ~~entry 1IN9~~ is ~~a Theoretical Model titled 'Homology-based Theoretical Molecular Model of Human Tryptophan Hydroxylase Regulatory~~, ~~Catalytic and Tetramerization Domains'~~.	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_1in9", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_1in9\| PDB=1in9 \| SCENE= }}

-	~~[[Category:Theoretical Model]]~~	+	===HOMOLOGY-BASED THEORETICAL MOLECULAR MODEL OF HUMAN TRYPTOPHAN HYDROXYLASE REGULATORY, CATALYTIC AND TETRAMERIZATION DOMAINS===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30 09~~:50:~~12 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_10993738}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10993738 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10993738}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:10993738</ref><references group="xtra"/>
		+	[[Category: Jiang, G C.T]]
		+	[[Category: Schmitt, J D]]
		+	[[Category: Vrana, K E]]
		+	[[Category: Yohrling Iv, G J]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:44:27 2010''