1ix8 - Revision history

OCA at 23:38, 27 December 2023

2023-12-27T23:38:37Z

←Older revision		Revision as of 23:38, 27 December 2023
Line 3:		Line 3:
	<StructureSection load='1ix8' size='340' side='right'caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ix8' size='340' side='right'caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PLP:PYRIDOXAL~~-~~5-PHOSPHATE'>PLP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1ix6\|1ix6]], [[1ix7\|1ix7]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [https://pdbe.org/1ix8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ix8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ix8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [https://pdbe.org/1ix8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ix8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ix8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AAT_ECOLI AAT_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Aspartate transaminase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hayashi, H]]	+	[[Category: Hayashi H]]
-	[[Category: Hirotsu, K]]	+	[[Category: Hirotsu K]]
-	[[Category: Kagamiyama, H]]	+	[[Category: Kagamiyama H]]
-	[[Category: Miyahara, I]]	+	[[Category: Miyahara I]]
-	[[Category: Mizuguchi, H]]	+	[[Category: Mizuguchi H]]
-	[[Category: Nakajima, Y]]	+	[[Category: Nakajima Y]]
-	~~[[Category: Active site mutant]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:14, 14 April 2021

2021-04-14T07:14:46Z

←Older revision		Revision as of 07:14, 14 April 2021
Line 3:		Line 3:
	<StructureSection load='1ix8' size='340' side='right'caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ix8' size='340' side='right'caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ix6\|1ix6]], [[1ix7\|1ix7]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [~~http~~://pdbe.org/1ix8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ix8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ix8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [https://pdbe.org/1ix8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ix8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ix8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:13, 10 July 2019

2019-07-10T07:13:32Z

←Older revision		Revision as of 07:13, 10 July 2019
Line 1:		Line 1:

	==Aspartate Aminotransferase Active Site Mutant V39F/N194A==		==Aspartate Aminotransferase Active Site Mutant V39F/N194A==
-	<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1ix8' size='340' side='right'caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ix8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ix8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Aspartate ~~Aminotransferase~~\|Aspartate ~~Aminotransferase~~]]	+	*[[Aspartate aminotransferase 3D structures\|Aspartate aminotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
		+	[[Category: Large Structures]]
	[[Category: Hayashi, H]]		[[Category: Hayashi, H]]
	[[Category: Hirotsu, K]]		[[Category: Hirotsu, K]]

OCA at 09:44, 4 October 2017

2017-10-04T09:44:26Z

←Older revision		Revision as of 09:44, 4 October 2017
Line 1:		Line 1:
		+
	==Aspartate Aminotransferase Active Site Mutant V39F/N194A==		==Aspartate Aminotransferase Active Site Mutant V39F/N194A==
	<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://pdbe.org/1ix8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://pdbe.org/1ix8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ix8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ix8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:27, 12 September 2015

2015-09-12T02:27:24Z

←Older revision		Revision as of 02:27, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://pdbe.org/1ix8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ix8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
-	[[Category: Escherichia coli]]
	[[Category: Hayashi, H]]		[[Category: Hayashi, H]]
	[[Category: Hirotsu, K]]		[[Category: Hirotsu, K]]

OCA at 12:06, 2 January 2015

2015-01-02T12:06:27Z

←Older revision		Revision as of 12:06, 2 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Hayashi, H.]]	+	[[Category: Hayashi, H]]
-	[[Category: Hirotsu, K.]]	+	[[Category: Hirotsu, K]]
-	[[Category: Kagamiyama, H.]]	+	[[Category: Kagamiyama, H]]
-	[[Category: Miyahara, I.]]	+	[[Category: Miyahara, I]]
-	[[Category: Mizuguchi, H.]]	+	[[Category: Mizuguchi, H]]
-	[[Category: Nakajima, Y.]]	+	[[Category: Nakajima, Y]]
	[[Category: Active site mutant]]		[[Category: Active site mutant]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:22, 28 September 2014

2014-09-28T14:22:51Z

←Older revision		Revision as of 14:22, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ix8.~~png~~\|~~left~~\|200px]]	+	==Aspartate Aminotransferase Active Site Mutant V39F/N194A==
		+	<StructureSection load='1ix8' size='340' side='right' caption='[[1ix8]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IX8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ix6\|1ix6]], [[1ix7\|1ix7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ix8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ix8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ix8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ix8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ix8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aspartate aminotransferase has been known to undergo a significant conformational change, in which the small domain approaches the large domain, and the residues at the entrance of the active site pack together, on binding of substrates. Accompanying this conformational change is a two-unit increase in the pK(a) of the pyridoxal 5'-phosphate-Lys(258) aldimine, which has been proposed to enhance catalysis. To elucidate how the conformational change is coupled to the shift in the aldimine pK(a) and how these changes are involved in catalysis, we analyzed structurally and kinetically an enzyme in which Val(39) located at both the domain interface and the entrance of the active site was replaced with a bulkier residue, Phe. The V39F mutant enzyme showed a more open conformation, and the aldimine pK(a) was lowered by 0.7 unit compared with the wild-type enzyme. When Asn(194) had been replaced by Ala in advance, the V39F mutation did not decrease the aldimine pK(a), showing that the domain rotation controls the aldimine pK(a) via the Arg(386)-Asn(194)-pyridoxal 5'-phosphate linkage system. The maleate-bound V39F enzyme showed the aldimine pK(a) 0.9 unit lower than that of the maleate-bound wild-type enzyme. However, the positions of maleate, Asn(194), and Arg(386) were superimposable between the mutant and the wild-type enzymes; therefore, the domain rotation was not the cause of the lowered aldimine pK(a) value. The maleate-bound V39F enzyme showed an altered side-chain packing pattern in the 37-39 region, and the lack of repulsion between Gly(38) carbonyl O and Tyr(225) Oeta seemed to be the cause of the reduced pK(a) value. Kinetic analysis suggested that the repulsion increases the free energy level of the Michaelis complex and promotes the catalytic reaction.

-	~~{{STRUCTURE_1ix8\| PDB=1ix8 \| SCENE= }}~~	+	Conformational change in aspartate aminotransferase on substrate binding induces strain in the catalytic group and enhances catalysis.,Hayashi H, Mizuguchi H, Miyahara I, Nakajima Y, Hirotsu K, Kagamiyama H J Biol Chem. 2003 Mar 14;278(11):9481-8. Epub 2002 Dec 17. PMID:12488449<ref>PMID:12488449</ref>

-	~~===Aspartate Aminotransferase Active Site Mutant V39F~~/~~N194A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12488449}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ix8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA].~~	+

	==See Also==		==See Also==
	*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]		*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012488449<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 22:30, 20 October 2012

2012-10-20T22:30:42Z

←Older revision		Revision as of 22:30, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ix8]] is a 1 chain structure ~~of [[Aspartate Aminotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA].	+	[[1ix8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA].

	==See Also==		==See Also==

OCA: Protected "1ix8" [edit=sysop:move=sysop]

2012-07-27T16:32:54Z

Protected "1ix8" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:32, 27 July 2012

OCA at 16:32, 27 July 2012

2012-07-27T16:32:52Z

←Older revision		Revision as of 16:32, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ix8.png\|left\|200px]]		[[Image:1ix8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ix8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ix8\| PDB=1ix8 \| SCENE= }}		{{STRUCTURE_1ix8\| PDB=1ix8 \| SCENE= }}

	===Aspartate Aminotransferase Active Site Mutant V39F/N194A===		===Aspartate Aminotransferase Active Site Mutant V39F/N194A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12488449}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12488449 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12488449}}		{{ABSTRACT_PUBMED_12488449}}

	==About this Structure==		==About this Structure==
-	~~1IX8~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA].	+	[[1ix8]] is a 1 chain structure of [[Aspartate Aminotransferase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IX8 OCA].
		+
		+	==See Also==
		+	*[[Aspartate Aminotransferase\|Aspartate Aminotransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12488449~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012488449</ref><references group="xtra"/>
	[[Category: Aspartate transaminase]]		[[Category: Aspartate transaminase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 33:		Line 24:
	[[Category: Nakajima, Y.]]		[[Category: Nakajima, Y.]]
	[[Category: Active site mutant]]		[[Category: Active site mutant]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 10:01:00 2009''~~	+