1jat - Revision history

OCA at 07:38, 7 February 2024

2024-02-07T07:38:20Z

←Older revision		Revision as of 07:38, 7 February 2024
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	<StructureSection load='1jat' size='340' side='right'caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1jat' size='340' side='right'caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1JAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin~~-~~-protein_ligase Ubiquitin--protein ligase]~~, ~~with EC number~~ [~~http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=~~6~~.3.2.19 6.3.2.19] </span>~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [https://pdbe.org/1jat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [https://www.ebi.ac.uk/pdbsum/1jat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [~~http~~://pdbe.org/1jat PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1jat PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBC13_YEAST UBC13_YEAST]] Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. [[http://www.uniprot.org/uniprot/MMS2_YEAST MMS2_YEAST]] Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.	+	[https://www.uniprot.org/uniprot/UBC13_YEAST UBC13_YEAST] Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jat ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains, little is known concerning the molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins. Mutations that destabilize the heterodimer interface confer a marked UV sensitivity, providing direct evidence that the intact heterodimer is necessary for DNA repair. Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals.
-
-	Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer.,VanDemark AP, Hofmann RM, Tsui C, Pickart CM, Wolberger C Cell. 2001 Jun 15;105(6):711-20. PMID:11440714<ref>PMID:11440714</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1jat" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Ubiquitin~~ conjugating enzyme\|~~Ubiquitin~~ conjugating enzyme]]	+	*[[3D structures of ubiquitin conjugating enzyme\|3D structures of ubiquitin conjugating enzyme]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ubiquitin--protein ligase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hofmann~~, R M~~]]	+	[[Category: Hofmann RM]]
-	[[Category: Pickart~~, C M~~]]	+	[[Category: Pickart CM]]
-	[[Category: Tsui, C]]	+	[[Category: Tsui C]]
-	[[Category: VanDemark~~, A P~~]]	+	[[Category: VanDemark AP]]
-	[[Category: Wolberger, C]]	+	[[Category: Wolberger C]]
-	~~[[Category: E2]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Ubiquitin]]~~	+
-	~~[[Category: Ubiquitin-conjugating enzyme]]~~	+
-	~~[[Category: Uev~~]]	+

OCA at 07:29, 25 November 2020

2020-11-25T07:29:19Z

←Older revision		Revision as of 07:29, 25 November 2020
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	==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==		==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==
-	<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1jat' size='340' side='right'caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1JAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://pdbe.org/1jat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://pdbe.org/1jat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jat_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jat_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Hofmann, R M]]		[[Category: Hofmann, R M]]

OCA at 09:44, 4 October 2017

2017-10-04T09:44:27Z

←Older revision		Revision as of 09:44, 4 October 2017
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		+
	==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==		==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==
	<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>
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	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://pdbe.org/1jat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://pdbe.org/1jat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:25, 11 September 2015

2015-09-11T15:25:35Z

←Older revision		Revision as of 15:25, 11 September 2015
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	<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://pdbe.org/1jat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1jat" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Hofmann, R M]]		[[Category: Hofmann, R M]]

OCA at 18:22, 25 December 2014

2014-12-25T18:22:27Z

←Older revision		Revision as of 18:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JAT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBC13_YEAST UBC13_YEAST]] Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. [[http://www.uniprot.org/uniprot/MMS2_YEAST MMS2_YEAST]] Has a role in the DNA error-free postreplication repair (PRR) pathway. Lacks catalytic activity by itself. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
-	[[Category: Hofmann, R M.]]	+	[[Category: Hofmann, R M]]
-	[[Category: Pickart, C M.]]	+	[[Category: Pickart, C M]]
-	[[Category: Tsui, C.]]	+	[[Category: Tsui, C]]
-	[[Category: VanDemark, A P.]]	+	[[Category: VanDemark, A P]]
-	[[Category: Wolberger, C.]]	+	[[Category: Wolberger, C]]
	[[Category: E2]]		[[Category: E2]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 13:31, 28 September 2014

2014-09-28T13:31:22Z

←Older revision		Revision as of 13:31, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1jat.~~png\|left~~\|200px]]	+	==Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex==
		+	<StructureSection load='1jat' size='340' side='right' caption='[[1jat]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JAT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), Mms2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jat RCSB], [http://www.ebi.ac.uk/pdbsum/1jat PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jat_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains, little is known concerning the molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins. Mutations that destabilize the heterodimer interface confer a marked UV sensitivity, providing direct evidence that the intact heterodimer is necessary for DNA repair. Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals.

-	~~{{STRUCTURE_1jat\| PDB=1jat \| SCENE= }}~~	+	Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer.,VanDemark AP, Hofmann RM, Tsui C, Pickart CM, Wolberger C Cell. 2001 Jun 15;105(6):711-20. PMID:11440714<ref>PMID:11440714</ref>

-	~~===Mms2~~/~~Ubc13 Ubiquitin Conjugating Enzyme Complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_11440714}}~~	+	==See Also==
-		+	*[[Ubiquitin conjugating enzyme\|Ubiquitin conjugating enzyme]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1jat~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:011440714<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]

OCA: Protected "1jat" [edit=sysop:move=sysop]

2012-12-05T11:16:24Z

Protected "1jat" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:16, 5 December 2012

OCA at 11:16, 5 December 2012

2012-12-05T11:16:19Z

←Older revision		Revision as of 11:16, 5 December 2012
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-	{{Seed}}
	[[Image:1jat.png\|left\|200px]]		[[Image:1jat.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1jat", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1jat\| PDB=1jat \| SCENE= }}		{{STRUCTURE_1jat\| PDB=1jat \| SCENE= }}

	===Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex===		===Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11440714}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11440714 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11440714}}		{{ABSTRACT_PUBMED_11440714}}

	==About this Structure==		==About this Structure==
-	~~1JAT~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].	+	[[1jat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11440714~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011440714</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
Line 32:		Line 20:
	[[Category: Wolberger, C.]]		[[Category: Wolberger, C.]]
	[[Category: E2]]		[[Category: E2]]
		+	[[Category: Ligase]]
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]
	[[Category: Ubiquitin-conjugating enzyme]]		[[Category: Ubiquitin-conjugating enzyme]]
	[[Category: Uev]]		[[Category: Uev]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:06:16 2009''

OCA at 00:06, 18 February 2009

2009-02-18T00:06:20Z

←Older revision		Revision as of 00:06, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1JAT is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].	+	1JAT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAT OCA].

	==Reference==		==Reference==
-	~~Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer., VanDemark AP, Hofmann RM, Tsui C, Pickart CM, Wolberger C, Cell. 2001 Jun 15;105(6):711-20.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11440714 11440714]~~	+	<ref group="xtra">PMID:11440714</ref><references group="xtra"/>
-	~~[[Category: Protein complex]]~~	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
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	[[Category: Uev]]		[[Category: Uev]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1~~ 18:54:~~48 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:06:16 2009''

OCA at 15:57, 1 July 2008

2008-07-01T15:57:22Z

←Older revision		Revision as of 15:57, 1 July 2008
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-	[[Image:1jat.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1jat.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1jat\| PDB=1jat \| SCENE= }}		{{STRUCTURE_1jat\| PDB=1jat \| SCENE= }}

-	~~'''~~Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex~~'''~~	+	===Mms2/Ubc13 Ubiquitin Conjugating Enzyme Complex===


-	~~==Overview==~~	+	<!--
-	~~While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains~~, ~~little is known concerning~~ the ~~molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in~~ the ~~assembly of Lys63-linked polyubiquitin chains that act~~ as ~~a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2~~/~~Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins~~. ~~Mutations that destabilize the heterodimer interface confer a marked UV sensitivity~~, ~~providing direct evidence that~~ the ~~intact heterodimer is necessary for DNA repair~~. ~~Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63~~-~~linked polyubiquitin signals.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_11440714}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 11440714 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_11440714}}

	==About this Structure==		==About this Structure==
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	[[Category: Ubiquitin-conjugating enzyme]]		[[Category: Ubiquitin-conjugating enzyme]]
	[[Category: Uev]]		[[Category: Uev]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 20~~:59:33 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 18:54:48 2008''