1jt8 - Revision history

OCA at 08:38, 22 May 2024

2024-05-22T08:38:50Z

←Older revision		Revision as of 08:38, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Translation initiation factor 1A (aIF-1A) from the archaeon Methanococcus jannaschii was expressed in Escherichia coli, purified, and characterized in terms of its structure and dynamics using multidimensional NMR methods. The protein was found to be a member of the OB-fold family of RNA-associated proteins, containing a barrel of five beta-strands, a feature that is shared with the homologous eukaryotic translation initiation factor 1A (eIF-1A), as well as the prokaryotic translation initiation factor IF1. External to the beta barrel, aIF-1A contains an alpha-helix at its C-terminal and a flexible loop at its N-terminal, features that are qualitatively similar to those found in eIF-1A, but not present in prokaryotic IF1. The structural model of aIF-1A, when used in combination with primary sequence information for aIF-1A in divergent species, permitted the most-conserved residues on the protein surface to be identified, including the most likely candidates for direct interaction with the 16S ribosomal RNA and other components of the translational apparatus. Several of the conserved surface residues appear to be unique to the archaea. Nitrogen-15 relaxation and amide exchange rate data were used to characterize the internal motions within aIF-1A, providing evidence that the protein surfaces that are most likely to participate in intermolecular interactions are relatively flexible. A model is proposed, suggesting some specific interactions that may occur between aIF-1A and the small subunit of the archaeal ribosome.
		+
		+	Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy.,Li W, Hoffman DW Protein Sci. 2001 Dec;10(12):2426-38. PMID:11714910<ref>PMID:11714910</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1jt8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:54, 3 April 2024

2024-04-03T07:54:50Z

←Older revision		Revision as of 07:54, 3 April 2024
Line 1:		Line 1:

	==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==		==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==
-	<StructureSection load='1jt8' size='340' side='right'caption='[[1jt8~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1jt8' size='340' side='right'caption='[[1jt8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43067 Atcc 43067~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [https://pdbe.org/1jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [https://pdbe.org/1jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IF1A_METJA IF1A_METJA]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).	+	[https://www.uniprot.org/uniprot/IF1A_METJA IF1A_METJA] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Translation initiation factor 1A (aIF-1A) from the archaeon Methanococcus jannaschii was expressed in Escherichia coli, purified, and characterized in terms of its structure and dynamics using multidimensional NMR methods. The protein was found to be a member of the OB-fold family of RNA-associated proteins, containing a barrel of five beta-strands, a feature that is shared with the homologous eukaryotic translation initiation factor 1A (eIF-1A), as well as the prokaryotic translation initiation factor IF1. External to the beta barrel, aIF-1A contains an alpha-helix at its C-terminal and a flexible loop at its N-terminal, features that are qualitatively similar to those found in eIF-1A, but not present in prokaryotic IF1. The structural model of aIF-1A, when used in combination with primary sequence information for aIF-1A in divergent species, permitted the most-conserved residues on the protein surface to be identified, including the most likely candidates for direct interaction with the 16S ribosomal RNA and other components of the translational apparatus. Several of the conserved surface residues appear to be unique to the archaea. Nitrogen-15 relaxation and amide exchange rate data were used to characterize the internal motions within aIF-1A, providing evidence that the protein surfaces that are most likely to participate in intermolecular interactions are relatively flexible. A model is proposed, suggesting some specific interactions that may occur between aIF-1A and the small subunit of the archaeal ribosome.
-
-	Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy.,Li W, Hoffman DW Protein Sci. 2001 Dec;10(12):2426-38. PMID:11714910<ref>PMID:11714910</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1jt8" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43067]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hoffman, D W~~]]	+	[[Category: Methanocaldococcus jannaschii]]
-	[[Category: ~~Li, W~~]]	+	[[Category: Hoffman DW]]
-	[[Category: ~~Beta barrel]]~~	+	[[Category: Li W]]
-	~~[[Category: Translation]]~~	+
-	~~[[Category: Translation factor~~]]	+

OCA at 06:45, 11 August 2021

2021-08-11T06:45:17Z

←Older revision		Revision as of 06:45, 11 August 2021
Line 3:		Line 3:
	<StructureSection load='1jt8' size='340' side='right'caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1jt8' size='340' side='right'caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [~~http~~://pdbe.org/1jt8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1jt8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt8 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [https://pdbe.org/1jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/IF1A_METJA IF1A_METJA]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).	+	[[https://www.uniprot.org/uniprot/IF1A_METJA IF1A_METJA]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:23, 6 November 2019

2019-11-06T09:23:33Z

←Older revision		Revision as of 09:23, 6 November 2019
Line 1:		Line 1:

	==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==		==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==
-	<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1jt8' size='340' side='right'caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43067]]		[[Category: Atcc 43067]]
		+	[[Category: Large Structures]]
	[[Category: Hoffman, D W]]		[[Category: Hoffman, D W]]
	[[Category: Li, W]]		[[Category: Li, W]]

OCA at 08:59, 17 January 2018

2018-01-17T08:59:17Z

←Older revision		Revision as of 08:59, 17 January 2018
Line 1:		Line 1:
		+
	==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==		==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==
	<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://pdbe.org/1jt8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://pdbe.org/1jt8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:16, 9 February 2016

2016-02-09T06:16:54Z

←Older revision		Revision as of 06:16, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:25, 10 September 2015

2015-09-10T19:25:29Z

←Older revision		Revision as of 19:25, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Methanocaldococcus_jannaschii Methanocaldococcus jannaschii~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://pdbe.org/1jt8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1jt8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Methanocaldococcus jannaschii~~]]	+	[[Category: Atcc 43067]]
	[[Category: Hoffman, D W]]		[[Category: Hoffman, D W]]
	[[Category: Li, W]]		[[Category: Li, W]]

OCA at 04:57, 25 December 2014

2014-12-25T04:57:52Z

←Older revision		Revision as of 04:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IF1A_METJA IF1A_METJA]] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Methanocaldococcus jannaschii]]		[[Category: Methanocaldococcus jannaschii]]
-	[[Category: Hoffman, D W.]]	+	[[Category: Hoffman, D W]]
-	[[Category: Li, W.]]	+	[[Category: Li, W]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Translation]]		[[Category: Translation]]
	[[Category: Translation factor]]		[[Category: Translation factor]]

OCA at 11:59, 28 September 2014

2014-09-28T11:59:49Z

←Older revision		Revision as of 11:59, 28 September 2014
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-	[[~~Image~~:1jt8.~~png\|left~~\|200px]]	+	==ARCHAEAL INITIATION FACTOR-1A, AIF-1A==
		+	<StructureSection load='1jt8' size='340' side='right' caption='[[1jt8]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1jt8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt8 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Translation initiation factor 1A (aIF-1A) from the archaeon Methanococcus jannaschii was expressed in Escherichia coli, purified, and characterized in terms of its structure and dynamics using multidimensional NMR methods. The protein was found to be a member of the OB-fold family of RNA-associated proteins, containing a barrel of five beta-strands, a feature that is shared with the homologous eukaryotic translation initiation factor 1A (eIF-1A), as well as the prokaryotic translation initiation factor IF1. External to the beta barrel, aIF-1A contains an alpha-helix at its C-terminal and a flexible loop at its N-terminal, features that are qualitatively similar to those found in eIF-1A, but not present in prokaryotic IF1. The structural model of aIF-1A, when used in combination with primary sequence information for aIF-1A in divergent species, permitted the most-conserved residues on the protein surface to be identified, including the most likely candidates for direct interaction with the 16S ribosomal RNA and other components of the translational apparatus. Several of the conserved surface residues appear to be unique to the archaea. Nitrogen-15 relaxation and amide exchange rate data were used to characterize the internal motions within aIF-1A, providing evidence that the protein surfaces that are most likely to participate in intermolecular interactions are relatively flexible. A model is proposed, suggesting some specific interactions that may occur between aIF-1A and the small subunit of the archaeal ribosome.

-	~~{{STRUCTURE_1jt8\| PDB=1jt8 \| SCENE= }}~~	+	Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy.,Li W, Hoffman DW Protein Sci. 2001 Dec;10(12):2426-38. PMID:11714910<ref>PMID:11714910</ref>

-	~~===ARCHAEAL INITIATION FACTOR-1A~~, ~~AIF-1A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11714910}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1jt8]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1JT8 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011714910<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Methanocaldococcus jannaschii]]		[[Category: Methanocaldococcus jannaschii]]
	[[Category: Hoffman, D W.]]		[[Category: Hoffman, D W.]]

OCA: Protected "1jt8" [edit=sysop:move=sysop]

2012-12-05T11:09:07Z

Protected "1jt8" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:09, 5 December 2012