1jt9 - Revision history

OCA at 07:15, 25 October 2023

2023-10-25T07:15:49Z

←Older revision		Revision as of 07:15, 25 October 2023
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	<StructureSection load='1jt9' size='340' side='right'caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='1jt9' size='340' side='right'caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1cd5\|1cd5]]~~, [[~~1fs6~~\|~~1fs6~~]]~~, [[1fsf\|1fsf]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.06Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en~~.~~wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [https://pdbe.org/1jt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [https://pdbe.org/1jt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NAGB_ECOLI NAGB_ECOLI]] Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.[HAMAP-Rule:MF_01241]	+	[https://www.uniprot.org/uniprot/NAGB_ECOLI NAGB_ECOLI] Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.[HAMAP-Rule:MF_01241]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Glucosamine-6-phosphate deaminase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bustos-Jaimes, I]]	+	[[Category: Bustos-Jaimes I]]
-	[[Category: Calcagno~~, M L~~]]	+	[[Category: Calcagno ML]]
-	[[Category: Horjales, E]]	+	[[Category: Horjales E]]
-	[[Category: Rudino-Pinera, E]]	+	[[Category: Rudino-Pinera E]]
-	[[Category: Sosa-Peinado, A]]	+	[[Category: Sosa-Peinado A]]
-	~~[[Category: Aldose-ketose isomerase]]~~	+
-	~~[[Category: Allosteric enzyme]]~~	+
-	~~[[Category: Entropic effect]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Structural flexibility~~]]	+

OCA at 06:45, 11 August 2021

2021-08-11T06:45:20Z

←Older revision		Revision as of 06:45, 11 August 2021
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	<StructureSection load='1jt9' size='340' side='right'caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='1jt9' size='340' side='right'caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [~~http~~://pdbe.org/1jt9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1jt9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [https://pdbe.org/1jt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NAGB_ECOLI NAGB_ECOLI]] Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.[HAMAP-Rule:MF_01241]	+	[[https://www.uniprot.org/uniprot/NAGB_ECOLI NAGB_ECOLI]] Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.[HAMAP-Rule:MF_01241]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:42, 6 November 2019

2019-11-06T09:42:24Z

←Older revision		Revision as of 09:42, 6 November 2019
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	==Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli==		==Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli==
-	<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='1jt9' size='340' side='right'caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1jt9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1jt9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Deaminase 3D structures\|Deaminase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Glucosamine-6-phosphate deaminase]]		[[Category: Glucosamine-6-phosphate deaminase]]
		+	[[Category: Large Structures]]
	[[Category: Bustos-Jaimes, I]]		[[Category: Bustos-Jaimes, I]]
	[[Category: Calcagno, M L]]		[[Category: Calcagno, M L]]

OCA at 09:17, 17 January 2018

2018-01-17T09:17:47Z

←Older revision		Revision as of 09:17, 17 January 2018
Line 1:		Line 1:
		+
	==Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli==		==Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli==
	<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://pdbe.org/1jt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://pdbe.org/1jt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1jt9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1jt9" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Deaminase\|Deaminase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:13, 8 February 2016

2016-02-08T12:13:40Z

←Older revision		Revision as of 12:13, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:28, 10 September 2015

2015-09-10T11:28:03Z

←Older revision		Revision as of 11:28, 10 September 2015
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	<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://pdbe.org/1jt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1jt9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Glucosamine-6-phosphate deaminase]]		[[Category: Glucosamine-6-phosphate deaminase]]
	[[Category: Bustos-Jaimes, I]]		[[Category: Bustos-Jaimes, I]]

OCA at 23:14, 24 December 2014

2014-12-24T23:14:03Z

←Older revision		Revision as of 23:14, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NAGB_ECOLI NAGB_ECOLI]] Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.[HAMAP-Rule:MF_01241]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Glucosamine-6-phosphate deaminase]]		[[Category: Glucosamine-6-phosphate deaminase]]
-	[[Category: Bustos-Jaimes, I.]]	+	[[Category: Bustos-Jaimes, I]]
-	[[Category: Calcagno, M L.]]	+	[[Category: Calcagno, M L]]
-	[[Category: Horjales, E.]]	+	[[Category: Horjales, E]]
-	[[Category: Rudino-Pinera, E.]]	+	[[Category: Rudino-Pinera, E]]
-	[[Category: Sosa-Peinado, A.]]	+	[[Category: Sosa-Peinado, A]]
	[[Category: Aldose-ketose isomerase]]		[[Category: Aldose-ketose isomerase]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]

OCA at 09:46, 28 September 2014

2014-09-28T09:46:49Z

←Older revision		Revision as of 09:46, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1jt9.~~png~~\|~~left~~\|200px]]	+	==Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli==
		+	<StructureSection load='1jt9' size='340' side='right' caption='[[1jt9]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JT9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cd5\|1cd5]], [[1fs6\|1fs6]], [[1fsf\|1fsf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jt9 RCSB], [http://www.ebi.ac.uk/pdbsum/1jt9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The active site of glucosamine-6-phosphate deaminase from Escherichia coli (GlcN6P deaminase, EC 3.5.99.6) has a complex lid formed by two antiparallel beta-strands connected by a helix-loop segment (158-187). This motif contains Arg172, which is a residue involved in binding the substrate in the active-site, and three residues that are part of the allosteric site, Arg158, Lys160 and Thr161. This dual binding role of the motif forming the lid suggests that it plays a key role in the functional coupling between active and allosteric sites. Previous crystallographic work showed that the temperature coefficients of the active-site lid are very large when the enzyme is in its T allosteric state. These coefficients decrease in the R state, thus suggesting that this motif changes its conformational flexibility as a consequence of the allosteric transition. In order to explore the possible connection between the conformational flexibility of the lid and the function of the deaminase, we constructed the site-directed mutant Phe174-Ala. Phe174 is located at the C-end of the lid helix and its side-chain establishes hydrophobic interactions with the remainder of the enzyme. The crystallographic structure of the T state of Phe174-Ala deaminase, determined at 2.02 A resolution, shows no density for the segment 162-181, which is part of the active-site lid (PDB 1JT9). This mutant form of the enzyme is essentially inactive in the absence of the allosteric activator, N-acetylglucosamine-6-P although it recovers its activity up to the wild-type level in the presence of this ligand. Spectrometric and binding studies show that inactivity is due to the inability of the active-site to bind ligands when the allosteric site is empty. These data indicate that the conformational flexibility of the active-site lid critically alters the binding properties of the active site, and that the occupation of the allosteric site restores the lid conformational flexibility to a functional state.

-	~~{{STRUCTURE_1jt9\| PDB=1jt9 \| SCENE= }}~~	+	On the role of the conformational flexibility of the active-site lid on the allosteric kinetics of glucosamine-6-phosphate deaminase.,Bustos-Jaimes I, Sosa-Peinado A, Rudino-Pinera E, Horjales E, Calcagno ML J Mol Biol. 2002 May 24;319(1):183-9. PMID:12051945<ref>PMID:12051945</ref>

-	~~===Structure~~ of the ~~mutant F174A T form~~ of ~~the Glucosamine-6-Phosphate deaminase from E~~.~~coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12051945}}~~	+	==See Also==
-		+	*[[Deaminase\|Deaminase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1jt9~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012051945<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Glucosamine-6-phosphate deaminase]]		[[Category: Glucosamine-6-phosphate deaminase]]

OCA: Protected "1jt9" [edit=sysop:move=sysop]

2012-12-05T11:32:37Z

Protected "1jt9" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:32, 5 December 2012

OCA at 11:32, 5 December 2012

2012-12-05T11:32:33Z

←Older revision		Revision as of 11:32, 5 December 2012
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-	{{Seed}}
	[[Image:1jt9.png\|left\|200px]]		[[Image:1jt9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1jt9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1jt9\| PDB=1jt9 \| SCENE= }}		{{STRUCTURE_1jt9\| PDB=1jt9 \| SCENE= }}

	===Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli===		===Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12051945}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12051945 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_12051945}}		{{ABSTRACT_PUBMED_12051945}}

	==About this Structure==		==About this Structure==
-	~~1JT9~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA].	+	[[1jt9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12051945~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012051945</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Glucosamine-6-phosphate deaminase]]		[[Category: Glucosamine-6-phosphate deaminase]]
Line 34:		Line 22:
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Entropic effect]]		[[Category: Entropic effect]]
		+	[[Category: Hydrolase]]
	[[Category: Structural flexibility]]		[[Category: Structural flexibility]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:55:01 2009''