1jwn - Revision history

OCA at 07:42, 7 February 2024

2024-02-07T07:42:34Z

←Older revision		Revision as of 07:42, 7 February 2024
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	<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anadara_inaequivalvis Anadara inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3sdh\|3sdh]]~~, ~~[[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [https://pdbe.org/1jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [https://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [https://pdbe.org/1jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [https://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLB1_ANAIN GLB1_ANAIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cooperative ligand binding in the dimeric hemoglobin from the blood clam Scapharca inaequivalvis results primarily from tertiary, rather than quaternary, structural changes. Ligand binding is coupled with conformational changes of key residues, including Phe 97, which is extruded from the proximal heme pocket, and the heme group, which moves deeper into the heme pocket. We have tested the role of the heme movement in cooperative function by mutating Ile 114, at the base of the heme pocket. Replacement of this residue with a Met did not disturb the hemoglobin structure or significantly alter equilibrium ligand binding properties. In contrast, substitution with a Phe at position 114 inhibits the ligand-linked movement of the heme group, and substantially reduces oxygen affinity and cooperativity. As the extent of heme movement to the normal position of the ligated state is diminished, Phe 97 is inhibited from its movement into the interface upon ligand binding. These results indicate a tight coupling between these two key cooperative transitions and suggest that the heme movement may be an obligatory trigger for expulsion of Phe 97 from the heme pocket.
-
-	Restricting the ligand-linked heme movement in Scapharca dimeric hemoglobin reveals tight coupling between distal and proximal contributions to cooperativity.,Knapp JE, Gibson QH, Cushing L, Royer WE Jr Biochemistry. 2001 Dec 11;40(49):14795-805. PMID:11732898<ref>PMID:11732898</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1jwn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]		*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Anadara inaequivalvis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cushing, L]]	+	[[Category: Cushing L]]
-	[[Category: Gibson~~, Q H~~]]	+	[[Category: Gibson QH]]
-	[[Category: Knapp~~, J E~~]]	+	[[Category: Knapp JE]]
-	[[Category: Royer~~, W E]]~~	+	[[Category: Royer Jr WE]]
-	~~[[Category: Allostery]]~~	+
-	~~[[Category: Cooperativity]]~~	+
-	~~[[Category: Heme protein]]~~	+
-	~~[[Category: Invertebrate hemoglobin]]~~	+
-	~~[[Category: Oxygen storage-transport complex]]~~	+
-	~~[[Category: Oxygen-binding protein~~]]	+

OCA at 14:35, 27 October 2021

2021-10-27T14:35:56Z

←Older revision		Revision as of 14:35, 27 October 2021
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	<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Anain Anain]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</div></td></tr>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anain]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cushing, L]]		[[Category: Cushing, L]]

OCA at 08:43, 21 April 2021

2021-04-21T08:43:47Z

←Older revision		Revision as of 08:43, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anain Anain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1JWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anain Anain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [~~http~~://pdbe.org/1jwn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [https://pdbe.org/1jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [https://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 12:55, 17 July 2019

2019-07-17T12:55:31Z

←Older revision		Revision as of 12:55, 17 July 2019
Line 1:		Line 1:

	==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==		==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==
-	<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anain Anain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anain Anain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Anain]]		[[Category: Anain]]
		+	[[Category: Large Structures]]
	[[Category: Cushing, L]]		[[Category: Cushing, L]]
	[[Category: Gibson, Q H]]		[[Category: Gibson, Q H]]

OCA at 07:12, 11 October 2017

2017-10-11T07:12:57Z

←Older revision		Revision as of 07:12, 11 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==		==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==
	<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://pdbe.org/1jwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://pdbe.org/1jwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:08, 11 September 2015

2015-09-11T11:08:08Z

←Older revision		Revision as of 11:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Scapharca_inaequivalvis Scapharca inaequivalvis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anain Anain]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://pdbe.org/1jwn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1jwn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Scapharca inaequivalvis~~]]	+	[[Category: Anain]]
	[[Category: Cushing, L]]		[[Category: Cushing, L]]
	[[Category: Gibson, Q H]]		[[Category: Gibson, Q H]]

OCA at 12:00, 2 January 2015

2015-01-02T12:00:27Z

←Older revision		Revision as of 12:00, 2 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Scapharca inaequivalvis]]		[[Category: Scapharca inaequivalvis]]
-	[[Category: Cushing, L.]]	+	[[Category: Cushing, L]]
-	[[Category: Gibson, Q H.]]	+	[[Category: Gibson, Q H]]
-	[[Category: Knapp, J E.]]	+	[[Category: Knapp, J E]]
-	[[Category: Royer, W E.]]	+	[[Category: Royer, W E]]
	[[Category: Allostery]]		[[Category: Allostery]]
	[[Category: Cooperativity]]		[[Category: Cooperativity]]

OCA at 09:08, 28 September 2014

2014-09-28T09:08:22Z

←Older revision		Revision as of 09:08, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1jwn.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==
		+	<StructureSection load='1jwn' size='340' side='right' caption='[[1jwn]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3sdh\|3sdh]], [[4sdh\|4sdh]], [[1hbi\|1hbi]], [[2hbi\|2hbi]], [[3hbi\|3hbi]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [http://www.ebi.ac.uk/pdbsum/1jwn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cooperative ligand binding in the dimeric hemoglobin from the blood clam Scapharca inaequivalvis results primarily from tertiary, rather than quaternary, structural changes. Ligand binding is coupled with conformational changes of key residues, including Phe 97, which is extruded from the proximal heme pocket, and the heme group, which moves deeper into the heme pocket. We have tested the role of the heme movement in cooperative function by mutating Ile 114, at the base of the heme pocket. Replacement of this residue with a Met did not disturb the hemoglobin structure or significantly alter equilibrium ligand binding properties. In contrast, substitution with a Phe at position 114 inhibits the ligand-linked movement of the heme group, and substantially reduces oxygen affinity and cooperativity. As the extent of heme movement to the normal position of the ligated state is diminished, Phe 97 is inhibited from its movement into the interface upon ligand binding. These results indicate a tight coupling between these two key cooperative transitions and suggest that the heme movement may be an obligatory trigger for expulsion of Phe 97 from the heme pocket.

-	~~{{STRUCTURE_1jwn\| PDB=1jwn \| SCENE= }}~~	+	Restricting the ligand-linked heme movement in Scapharca dimeric hemoglobin reveals tight coupling between distal and proximal contributions to cooperativity.,Knapp JE, Gibson QH, Cushing L, Royer WE Jr Biochemistry. 2001 Dec 11;40(49):14795-805. PMID:11732898<ref>PMID:11732898</ref>

-	~~===Crystal Structure of Scapharca inaequivalvis HbI~~, ~~I114F Mutant Ligated to Carbon Monoxide.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11732898}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1jwn]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA].~~	+

	==See Also==		==See Also==
-	*[[Hemoglobin\|Hemoglobin]]	+	*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011732898<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Scapharca inaequivalvis]]		[[Category: Scapharca inaequivalvis]]
	[[Category: Cushing, L.]]		[[Category: Cushing, L.]]

OCA at 18:26, 20 October 2012

2012-10-20T18:26:26Z

←Older revision		Revision as of 18:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1jwn]] is a 4 chain structure ~~of [[Hemoglobin]]~~ with sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA].	+	[[1jwn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA].

	==See Also==		==See Also==

OCA: Protected "1jwn" [edit=sysop:move=sysop]

2012-07-27T04:05:13Z

Protected "1jwn" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 27 July 2012