1khk - Revision history

OCA at 08:58, 16 August 2023

2023-08-16T08:58:47Z

←Older revision		Revision as of 08:58, 16 August 2023
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	<StructureSection load='1khk' size='340' side='right'caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1khk' size='340' side='right'caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KHK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1alk\|1alk]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [https://pdbe.org/1khk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [https://www.ebi.ac.uk/pdbsum/1khk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1khk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [https://pdbe.org/1khk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [https://www.ebi.ac.uk/pdbsum/1khk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1khk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PPB_ECOLI PPB_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Alkaline phosphatase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boulain~~, J C~~]]	+	[[Category: Boulain JC]]
-	[[Category: Bulgakov~~, O V]]~~	+	[[Category: Bulgakov OV]]
-	~~[[Category: Du, M H.Le~~]]	+	[[Category: Lajeunesse E]]
-	[[Category: Lajeunesse, E]]	+	[[Category: Lamoure C]]
-	[[Category: Lamoure, C]]	+	[[Category: Le Du MH]]
-	[[Category: ~~Menez, A~~]]	+	[[Category: Menez A]]
-	[[Category: ~~Muller, B H~~]]	+	[[Category: Muller BH]]
-	[[Category: ~~Hydrolase~~]]	+

OCA at 14:41, 27 October 2021

2021-10-27T14:41:29Z

←Older revision		Revision as of 14:41, 27 October 2021
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	<StructureSection load='1khk' size='340' side='right'caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1khk' size='340' side='right'caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KHK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KHK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1alk\|1alk]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [~~http~~://pdbe.org/1khk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1khk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1khk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [https://pdbe.org/1khk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [https://www.ebi.ac.uk/pdbsum/1khk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1khk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:34, 22 January 2020

2020-01-22T17:34:51Z

←Older revision		Revision as of 17:34, 22 January 2020
Line 1:		Line 1:

	==E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)==		==E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)==
-	<StructureSection load='1khk' size='340' side='right' caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1khk' size='340' side='right'caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KHK FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Alkaline phosphatase\|Alkaline phosphatase]]	+	*[[Alkaline phosphatase 3D structures\|Alkaline phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
		+	[[Category: Large Structures]]
	[[Category: Boulain, J C]]		[[Category: Boulain, J C]]
	[[Category: Bulgakov, O V]]		[[Category: Bulgakov, O V]]

OCA at 06:15, 4 April 2018

2018-04-04T06:15:58Z

←Older revision		Revision as of 06:15, 4 April 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1khk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1khk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1khk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1khk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alkaline phosphatase\|Alkaline phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:12, 11 October 2017

2017-10-11T07:12:22Z

←Older revision		Revision as of 07:12, 11 October 2017
Line 1:		Line 1:
		+
	==E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)==		==E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)==
	<StructureSection load='1khk' size='340' side='right' caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1khk' size='340' side='right' caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://pdbe.org/1khk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://pdbe.org/1khk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1khk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1khk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 1khk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1khk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Alkaline phosphatase\|Alkaline phosphatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:45, 12 September 2015

2015-09-12T00:45:45Z

←Older revision		Revision as of 00:45, 12 September 2015
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://pdbe.org/1khk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1khk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:45, 5 January 2015

2015-01-05T16:45:08Z

←Older revision		Revision as of 16:45, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KHK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KHK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
-	[[Category: Boulain, J C.]]	+	[[Category: Boulain, J C]]
-	[[Category: Bulgakov, O V.]]	+	[[Category: Bulgakov, O V]]
-	[[Category: Du, M H.Le.]]	+	[[Category: Du, M H.Le]]
-	[[Category: Lajeunesse, E.]]	+	[[Category: Lajeunesse, E]]
-	[[Category: Lamoure, C.]]	+	[[Category: Lamoure, C]]
-	[[Category: Menez, A.]]	+	[[Category: Menez, A]]
-	[[Category: Muller, B H~~.]]~~	+	[[Category: Muller, B H]]
-	~~[[Category: Alkaline phosphatase~~]]	+
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:07, 25 June 2014

2014-06-25T05:07:18Z

←Older revision		Revision as of 05:07, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~1khk.~~png~~\|~~left~~\|200px]]	+	==E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)==
		+	<StructureSection load='1khk' size='340' side='right' caption='[[1khk]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KHK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1alk\|1alk]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1khk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1khk RCSB], [http://www.ebi.ac.uk/pdbsum/1khk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1khk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of three mutants of Escherichia coli alkaline phosphatase with catalytic activity (k(cat)) enhancement as compare to the wild-type enzyme is described in different states. The biological aspects of this study have been reported elsewhere. The structure of the first mutant, D330N, which is threefold more active than the wild-type enzyme, was determined with phosphate in the active site, or with aluminium fluoride, which mimics the transition state. These structures reveal, in particular, that this first mutation does not alter the active site. The second mutant, D153H-D330N, is 17-fold more active than the wild-type enzyme and activated by magnesium, but its activity drops after few days. The structure of this mutant was solved under four different conditions. The phosphate-free enzyme was studied in an inactivated form with zinc at site M3, or after activation by magnesium. The comparison of these two forms free of phosphate illustrates the mechanism of the magnesium activation of the catalytic serine residue. In the presence of magnesium, the structure was determined with phosphate, or aluminium fluoride. The drop in activity of the mutant D153H-D330N could be explained by the instability of the metal ion at M3. The analysis of this mutant helped in the design of the third mutant, D153G-D330N. This mutant is up to 40-fold more active than the wild-type enzyme, with a restored robustness of the enzyme stability. The structure is presented here with covalently bound phosphate in the active site, representing the first phosphoseryl intermediate of a highly active alkaline phosphatase. This study shows how structural analysis may help to progress in the improvement of an enzyme catalytic activity (k(cat)), and explains the structural events associated with this artificial evolution.

-	~~{{STRUCTURE_1khk\| PDB=1khk \| SCENE= }}~~	+	Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.,Le Du MH, Lamoure C, Muller BH, Bulgakov OV, Lajeunesse E, Menez A, Boulain JC J Mol Biol. 2002 Mar 1;316(4):941-53. PMID:11884134<ref>PMID:11884134</ref>

-	~~===E. COLI ALKALINE PHOSPHATASE MUTANT (D153HD330N)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11884134}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1khk]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA].~~	+

	==See Also==		==See Also==
	*[[Alkaline phosphatase\|Alkaline phosphatase]]		*[[Alkaline phosphatase\|Alkaline phosphatase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011884134<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
-	[[Category: Escherichia coli]]
	[[Category: Boulain, J C.]]		[[Category: Boulain, J C.]]
	[[Category: Bulgakov, O V.]]		[[Category: Bulgakov, O V.]]

OCA at 21:56, 20 October 2012

2012-10-20T21:56:19Z

←Older revision		Revision as of 21:56, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1khk]] is a 2 chain structure ~~of [[Alkaline phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA].	+	[[1khk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHK OCA].

	==See Also==		==See Also==

OCA: Protected "1khk" [edit=sysop:move=sysop]

2012-07-27T14:42:55Z

Protected "1khk" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:42, 27 July 2012