1kk4 - Revision history

OCA at 07:25, 14 February 2024

2024-02-14T07:25:38Z

←Older revision		Revision as of 07:25, 14 February 2024
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	<StructureSection load='1kk4' size='340' side='right'caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1kk4' size='340' side='right'caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_19434 Atcc 19434~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1kk5\|1kk5]], [[1kk6\|1kk6]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [https://pdbe.org/1kk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [https://www.ebi.ac.uk/pdbsum/1kk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [~~http~~://pdbe.org/1kk4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1kk4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/VATD_ENTFC VATD_ENTFC]] Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.	+	[https://www.uniprot.org/uniprot/VATD_ENTFC VATD_ENTFC] Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kk4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kk4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The streptogramin class of antibiotics act to inhibit bacterial protein synthesis, and their semisynthetic derivatives, such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of the nosocomial pathogen Enterococcus faecium to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA. These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base. The structural similarity of Vat(D) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. These results reveal the molecular basis for a reaction by which Gram-positive cocci acquire resistance to a last resort antibiotic.
-
-	Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics.,Sugantino M, Roderick SL Biochemistry. 2002 Feb 19;41(7):2209-16. PMID:11841212<ref>PMID:11841212</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1kk4" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 19434~~]]	+	[[Category: Enterococcus faecium]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Roderick~~, S L~~]]	+	[[Category: Roderick SL]]
-	[[Category: Sugantino, M]]	+	[[Category: Sugantino M]]
-	~~[[Category: Acyltransferase]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:46, 25 November 2020

2020-11-25T07:46:10Z

←Older revision		Revision as of 07:46, 25 November 2020
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	==Crystal Structure of Vat(D) in Complex with Acetyl-CoA==		==Crystal Structure of Vat(D) in Complex with Acetyl-CoA==
-	<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='1kk4' size='340' side='right'caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19434 Atcc 19434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19434 Atcc 19434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kk5\|1kk5]], [[1kk6\|1kk6]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://pdbe.org/1kk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://pdbe.org/1kk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kk4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kk4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 19434]]		[[Category: Atcc 19434]]
		+	[[Category: Large Structures]]
	[[Category: Roderick, S L]]		[[Category: Roderick, S L]]
	[[Category: Sugantino, M]]		[[Category: Sugantino, M]]

OCA at 07:22, 11 October 2017

2017-10-11T07:22:29Z

←Older revision		Revision as of 07:22, 11 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of Vat(D) in Complex with Acetyl-CoA==		==Crystal Structure of Vat(D) in Complex with Acetyl-CoA==
	<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://pdbe.org/1kk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://pdbe.org/1kk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kk4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kk4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:08, 11 September 2015

2015-09-11T09:08:06Z

←Older revision		Revision as of 09:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterococcus_faecium Enterococcus faecium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19434 Atcc 19434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ~~Enterococcus faecium~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 ATCC 19434])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://pdbe.org/1kk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1kk4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterococcus faecium~~]]	+	[[Category: Atcc 19434]]
	[[Category: Roderick, S L]]		[[Category: Roderick, S L]]
	[[Category: Sugantino, M]]		[[Category: Sugantino, M]]

OCA at 14:16, 25 December 2014

2014-12-25T14:16:33Z

←Older revision		Revision as of 14:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VATD_ENTFC VATD_ENTFC]] Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Enterococcus faecium]]		[[Category: Enterococcus faecium]]
-	[[Category: Roderick, S L.]]	+	[[Category: Roderick, S L]]
-	[[Category: Sugantino, M.]]	+	[[Category: Sugantino, M]]
	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:14, 28 September 2014

2014-09-28T13:14:34Z

←Older revision		Revision as of 13:14, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1kk4.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Vat(D) in Complex with Acetyl-CoA==
		+	<StructureSection load='1kk4' size='340' side='right' caption='[[1kk4]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KK4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kk5\|1kk5]], [[1kk6\|1kk6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">satA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kk4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1kk4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kk/1kk4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The streptogramin class of antibiotics act to inhibit bacterial protein synthesis, and their semisynthetic derivatives, such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of the nosocomial pathogen Enterococcus faecium to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA. These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base. The structural similarity of Vat(D) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. These results reveal the molecular basis for a reaction by which Gram-positive cocci acquire resistance to a last resort antibiotic.

-	~~{{STRUCTURE_1kk4\| PDB=1kk4 \| SCENE= }}~~	+	Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics.,Sugantino M, Roderick SL Biochemistry. 2002 Feb 19;41(7):2209-16. PMID:11841212<ref>PMID:11841212</ref>

-	~~===Crystal Structure~~ of ~~Vat(D) in Complex with Acetyl-CoA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11841212}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1kk4]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterococcus_faecium Enterococcus faecium]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011841212<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterococcus faecium]]		[[Category: Enterococcus faecium]]
	[[Category: Roderick, S L.]]		[[Category: Roderick, S L.]]

OCA: Protected "1kk4" [edit=sysop:move=sysop]

2012-12-05T19:47:18Z

Protected "1kk4" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:47, 5 December 2012

OCA at 19:47, 5 December 2012

2012-12-05T19:47:15Z

←Older revision		Revision as of 19:47, 5 December 2012
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-	{{Seed}}
	[[Image:1kk4.png\|left\|200px]]		[[Image:1kk4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1kk4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1kk4\| PDB=1kk4 \| SCENE= }}		{{STRUCTURE_1kk4\| PDB=1kk4 \| SCENE= }}

	===Crystal Structure of Vat(D) in Complex with Acetyl-CoA===		===Crystal Structure of Vat(D) in Complex with Acetyl-CoA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11841212}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11841212 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11841212}}		{{ABSTRACT_PUBMED_11841212}}

	==About this Structure==		==About this Structure==
-	~~1KK4~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA].	+	[[1kk4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11841212~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011841212</ref><references group="xtra"/>
	[[Category: Enterococcus faecium]]		[[Category: Enterococcus faecium]]
	[[Category: Roderick, S L.]]		[[Category: Roderick, S L.]]
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	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 18:27:34 2009''~~	+

OCA at 16:27, 17 February 2009

2009-02-17T16:27:36Z

←Older revision		Revision as of 16:27, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1KK4 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA].	+	1KK4 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KK4 OCA].

	==Reference==		==Reference==
-	~~Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin~~ group ~~A antibiotics., Sugantino M, Roderick SL, Biochemistry. 2002 Feb 19;41(7):2209-16.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11841212 11841212]~~	+	<ref group="xtra">PMID:11841212</ref><references group="xtra"/>
	[[Category: Enterococcus faecium]]		[[Category: Enterococcus faecium]]
-	[[Category: Single protein]]
	[[Category: Roderick, S L.]]		[[Category: Roderick, S L.]]
	[[Category: Sugantino, M.]]		[[Category: Sugantino, M.]]
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	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 2 10~~:27:~~33 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:27:34 2009''

OCA at 07:27, 2 July 2008

2008-07-02T07:27:36Z

←Older revision		Revision as of 07:27, 2 July 2008
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-	[[Image:1kk4.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1kk4.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1kk4\| PDB=1kk4 \| SCENE= }}		{{STRUCTURE_1kk4\| PDB=1kk4 \| SCENE= }}

-	~~'''~~Crystal Structure of Vat(D) in Complex with Acetyl-CoA~~'''~~	+	===Crystal Structure of Vat(D) in Complex with Acetyl-CoA===


-	~~==Overview==~~	+	<!--
-	The ~~streptogramin class of antibiotics act to inhibit bacterial protein synthesis~~, ~~and their semisynthetic derivatives~~, ~~such as dalfopristin-quinupristin (Synercid), are used to treat serious or life-threatening infections due to multiply antibiotic resistant bacteria. Acquired resistance of~~ the ~~nosocomial pathogen Enterococcus faecium~~ to the group A component of natural and semisynthetic streptogramin mixtures is a prerequisite for the streptogramin resistance phenotype and is mediated by a streptogramin acetyltransferase. The crystal structure of Vat(~~D), a streptogramin acetyltransferase from a human urinary isolate of E. faecium, has been determined~~ as ~~an apoenzyme and in complex with either acetyl-CoA or virginiamycin M1 and CoA~~. ~~These structures illustrate the location and arrangement of residues at the active site, and point to His 82 as a residue that may function as a general base~~. ~~The structural similarity of Vat(D~~) to the xenobiotic acetyltransferase from Pseudomonas aeruginosa indicates similarities in the catalytic mechanism for these enzymes as well as several shared and distinctive antibiotic binding interactions between these enzymes and their respective substrates. ~~These results reveal the molecular basis for a reaction by which Gram~~-~~positive cocci acquire resistance to a last resort antibiotic.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_11841212}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 11841212 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_11841212}}

	==About this Structure==		==About this Structure==
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	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 22:50:17 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:27:33 2008''