1kkv - Revision history

OCA at 18:46, 29 November 2023

2023-11-29T18:46:25Z

←Older revision		Revision as of 18:46, 29 November 2023
Line 1:		Line 1:

	==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==		==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==
-	<StructureSection load='1kkv' size='340' side='right'caption='[[1kkv~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1kkv' size='340' side='right'caption='[[1kkv]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1kkw\|1kkw]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [https://pdbe.org/1kkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [https://www.ebi.ac.uk/pdbsum/1kkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kkv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [https://pdbe.org/1kkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [https://www.ebi.ac.uk/pdbsum/1kkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kkv ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Isaacs~~, R J~~]]	+	[[Category: Isaacs RJ]]
-	[[Category: Spielmann~~, H P]]~~	+	[[Category: Spielmann HP]]
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Dynamic]]~~	+
-	~~[[Category: G-t mismatch]]~~	+
-	~~[[Category: Helical parameter]]~~	+
-	~~[[Category: Order parameter]]~~	+
-	~~[[Category: Sequence dependent dynamic]]~~	+
-	~~[[Category: Sequence dependent structure]]~~	+
-	~~[[Category: Spin relaxation~~]]	+

OCA at 08:48, 21 April 2021

2021-04-21T08:48:33Z

←Older revision		Revision as of 08:48, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1kkv' size='340' side='right'caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1kkv' size='340' side='right'caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KKV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1kkw\|1kkw]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [~~http~~://pdbe.org/1kkv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1kkv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1kkv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [https://pdbe.org/1kkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [https://www.ebi.ac.uk/pdbsum/1kkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kkv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:23, 17 July 2019

2019-07-17T13:23:33Z

←Older revision		Revision as of 13:23, 17 July 2019
Line 1:		Line 1:

	==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==		==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==
-	<StructureSection load='1kkv' size='340' side='right' caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1kkv' size='340' side='right'caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Isaacs, R J]]		[[Category: Isaacs, R J]]
	[[Category: Spielmann, H P]]		[[Category: Spielmann, H P]]

OCA at 07:15, 11 October 2017

2017-10-11T07:15:15Z

←Older revision		Revision as of 07:15, 11 October 2017
Line 1:		Line 1:
		+
	==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==		==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==
	<StructureSection load='1kkv' size='340' side='right' caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1kkv' size='340' side='right' caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://pdbe.org/1kkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://pdbe.org/1kkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kkv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:06, 9 September 2015

2015-09-09T22:06:11Z

←Older revision		Revision as of 22:06, 9 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://pdbe.org/1kkv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1kkv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:27, 22 December 2014

2014-12-22T06:27:36Z

←Older revision		Revision as of 06:27, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Isaacs, R J.]]	+	[[Category: Isaacs, R J]]
-	[[Category: Spielmann, H P.]]	+	[[Category: Spielmann, H P]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Dynamic]]		[[Category: Dynamic]]

OCA at 05:32, 8 June 2014

2014-06-08T05:32:12Z

←Older revision		Revision as of 05:32, 8 June 2014
Line 1:		Line 1:
-	[[~~Image:~~1kkv.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure==
		+	<StructureSection load='1kkv' size='340' side='right' caption='[[1kkv]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KKV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1kkw\|1kkw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kkv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kkv RCSB], [http://www.ebi.ac.uk/pdbsum/1kkv PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Detailed description of the characteristics of mismatched DNA that are distinct from normal DNA is vital to the understanding of how mismatch repair proteins are able to recognize and repair these DNA lesions. To this end, we have used nuclear Overhauser effect spectroscopy (NOESY)-based distance restraints and (13)C relaxation measurements to solve the solution structures and measure some of the internal dynamics of the G-T mismatched DNA oligomer d(CCATGCGTGG)(2) (GT) and its parent DNA sequence d(CCACGCGTGG)(2) (GC). In GT, the mismatched G7 is structurally perturbed much more than the mismatched T4 relative to their corresponding bases in GC. The degree of G7 displacement differs from previous high-resolution structures of G-T mismatch-containing B-DNA, suggesting a dependence of G-T mismatch-induced structural perturbation on sequence context. The internal dynamics of GC and GT differ on multiple timescales. The mismatched G7 of GT contains spins that decrease significantly in order in GT compared to GC, while spins in C6, T8, and A3 have significantly higher order in GT compared to GC. Linear correlations between helical parameters of GC and GT and the order of C-1' and aromatic methine carbon atoms relate differences in internal dynamics to the structures quantitatively. The dynamic differences between the normal and mismatched DNA signify changes in local flexibility that may be exploited by the mismatch repair system to bind mismatched DNA preferentially while ignoring normal DNA.

-	~~{{STRUCTURE_1kkv\| PDB=1kkv \| SCENE= }}~~	+	Structural differences in the NOE-derived structure of G-T mismatched DNA relative to normal DNA are correlated with differences in (13)C relaxation-based internal dynamics.,Isaacs RJ, Rayens WS, Spielmann HP J Mol Biol. 2002 May 24;319(1):191-207. PMID:12051946<ref>PMID:12051946</ref>

-	~~===NMR Solution Structure of d(CCACGCGTGG)2~~, ~~parent to G-T mismatch structure===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12051946}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1kkv]] is~~ a ~~2 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1KKV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012051946<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Isaacs, R J.]]		[[Category: Isaacs, R J.]]
	[[Category: Spielmann, H P.]]		[[Category: Spielmann, H P.]]

OCA: Protected "1kkv" [edit=sysop:move=sysop]

2012-08-14T18:34:28Z

Protected "1kkv" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:34, 14 August 2012

OCA at 18:34, 14 August 2012

2012-08-14T18:34:26Z

←Older revision		Revision as of 18:34, 14 August 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1kkv.png\|left\|200px]]		[[Image:1kkv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1kkv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1kkv\| PDB=1kkv \| SCENE= }}		{{STRUCTURE_1kkv\| PDB=1kkv \| SCENE= }}

	===NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure===		===NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12051946}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12051946 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12051946}}		{{ABSTRACT_PUBMED_12051946}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA].	+	[[1kkv]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA].

	==Reference==		==Reference==
-	Structural differences in the NOE-derived structure of G-T mismatched DNA relative to normal DNA are correlated with differences in (13)C relaxation-based internal dynamics., Isaacs RJ, Rayens WS, Spielmann HP, J Mol Biol. 2002 May 24;319(1):191-207. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~12051946 12051946]~~	+	<ref group="xtra">PMID:012051946</ref><references group="xtra"/>
	[[Category: Isaacs, R J.]]		[[Category: Isaacs, R J.]]
	[[Category: Spielmann, H P.]]		[[Category: Spielmann, H P.]]
		+	[[Category: Dna]]
	[[Category: Dynamic]]		[[Category: Dynamic]]
	[[Category: G-t mismatch]]		[[Category: G-t mismatch]]
Line 33:		Line 22:
	[[Category: Sequence dependent structure]]		[[Category: Sequence dependent structure]]
	[[Category: Spin relaxation]]		[[Category: Spin relaxation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:29:35 2008''

OCA at 07:29, 2 July 2008

2008-07-02T07:29:37Z

←Older revision		Revision as of 07:29, 2 July 2008
Line 1:		Line 1:
-	[[Image:1kkv.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1kkv.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1kkv\| PDB=1kkv \| SCENE= }}		{{STRUCTURE_1kkv\| PDB=1kkv \| SCENE= }}

-	~~'''~~NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure~~'''~~	+	===NMR Solution Structure of d(CCACGCGTGG)2, parent to G-T mismatch structure===


-	~~==Overview==~~	+	<!--
-	Detailed description of the characteristics of mismatched DNA that are distinct from normal DNA is vital to the understanding of how mismatch repair proteins are able to recognize and repair these DNA lesions. To this ~~end~~, ~~we have used nuclear Overhauser effect spectroscopy (NOESY)-based distance restraints and (13)C relaxation measurements~~ to ~~solve~~ the ~~solution structures and measure some of the internal dynamics of the G-T mismatched DNA oligomer d~~(~~CCATGCGTGG~~)~~(2) (GT) and its parent DNA sequence d(CCACGCGTGG)(2) (GC). In GT~~, ~~the mismatched G7~~ is ~~structurally perturbed much more than~~ the ~~mismatched T4 relative to their corresponding bases in GC~~. ~~The degree of G7 displacement differs from previous high~~-~~resolution structures of G~~-T mismatch-containing B-DNA, suggesting a dependence of G-T mismatch-induced structural perturbation on sequence context. The internal dynamics of GC and GT differ on multiple timescales. The mismatched G7 of GT contains spins that decrease significantly in order in GT compared to GC, while spins in C6, T8, and A3 have significantly higher order in GT compared to GC. Linear correlations between helical parameters of GC and GT and the order of C-1' and aromatic methine carbon atoms relate differences in internal dynamics to the structures quantitatively. The dynamic differences between the normal and mismatched DNA signify changes in local flexibility that may be exploited by the mismatch repair system to bind mismatched DNA preferentially while ignoring normal DNA.	+	The line below this paragraph, {{ABSTRACT_PUBMED_12051946}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 12051946 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_12051946}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KKV OCA].

	==Reference==		==Reference==
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	[[Category: Sequence dependent structure]]		[[Category: Sequence dependent structure]]
	[[Category: Spin relaxation]]		[[Category: Spin relaxation]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 22:51:58 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:29:35 2008''