1ko7 - Revision history

OCA at 09:01, 16 August 2023

2023-08-16T09:01:25Z

←Older revision		Revision as of 09:01, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='1ko7' size='340' side='right'caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1ko7' size='340' side='right'caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_29971 Atcc 29971~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1288 ATCC 29971])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [~~http~~://pdbe.org/1ko7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ko7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ko7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [https://pdbe.org/1ko7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ko7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ko7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HPRK_STAXY HPRK_STAXY]] Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-46' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, the HprK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.	+	[https://www.uniprot.org/uniprot/HPRK_STAXY HPRK_STAXY] Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-46' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, the HprK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 29971]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fieulaine, S]]	+	[[Category: Staphylococcus xylosus]]
-	[[Category: Hasenbein, S]]	+	[[Category: Fieulaine S]]
-	[[Category: Hengstenberg, W]]	+	[[Category: Hasenbein S]]
-	[[Category: Koch, B]]	+	[[Category: Hengstenberg W]]
-	[[Category: Marquez~~, J A~~]]	+	[[Category: Koch B]]
-	[[Category: Nessler, S]]	+	[[Category: Marquez JA]]
-	[[Category: Scheffzek, K]]	+	[[Category: Nessler S]]
-	~~[[Category: Dual activity]]~~	+	[[Category: Scheffzek K]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphotransfer]]~~	+
-	~~[[Category: Product]]~~	+
-	~~[[Category: Protein kinase]]~~	+
-	~~[[Category: Protein phosphatase]]~~	+
-	~~[[Category: Substrate]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:47, 25 November 2020

2020-11-25T07:47:42Z

←Older revision		Revision as of 07:47, 25 November 2020
Line 1:		Line 1:

	==X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution==		==X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution==
-	<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='1ko7' size='340' side='right'caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29971 Atcc 29971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29971 Atcc 29971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ATCC 29971])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ATCC 29971])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://pdbe.org/1ko7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ko7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://pdbe.org/1ko7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ko7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1ko7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1ko7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 29971]]		[[Category: Atcc 29971]]
		+	[[Category: Large Structures]]
	[[Category: Fieulaine, S]]		[[Category: Fieulaine, S]]
	[[Category: Hasenbein, S]]		[[Category: Hasenbein, S]]

OCA at 07:20, 11 October 2017

2017-10-11T07:20:04Z

←Older revision		Revision as of 07:20, 11 October 2017
Line 1:		Line 1:
		+
	==X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution==		==X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution==
	<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ATCC 29971])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ATCC 29971])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://pdbe.org/1ko7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://pdbe.org/1ko7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ko7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ko7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:21, 12 September 2015

2015-09-12T01:21:51Z

←Older revision		Revision as of 01:21, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_xylosus Staphylococcus xylosus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29971 Atcc 29971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ~~Staphylococcus xylosus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 ATCC 29971])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://pdbe.org/1ko7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ko7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus xylosus~~]]	+	[[Category: Atcc 29971]]
	[[Category: Fieulaine, S]]		[[Category: Fieulaine, S]]
	[[Category: Hasenbein, S]]		[[Category: Hasenbein, S]]

OCA at 21:56, 25 December 2014

2014-12-25T21:56:35Z

←Older revision		Revision as of 21:56, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 Staphylococcus xylosus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 Staphylococcus xylosus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HPRK_STAXY HPRK_STAXY]] Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-46' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, the HprK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus xylosus]]		[[Category: Staphylococcus xylosus]]
-	[[Category: Fieulaine, S.]]	+	[[Category: Fieulaine, S]]
-	[[Category: Hasenbein, S.]]	+	[[Category: Hasenbein, S]]
-	[[Category: Hengstenberg, W.]]	+	[[Category: Hengstenberg, W]]
-	[[Category: Koch, B.]]	+	[[Category: Koch, B]]
-	[[Category: Marquez, J A.]]	+	[[Category: Marquez, J A]]
-	[[Category: Nessler, S.]]	+	[[Category: Nessler, S]]
-	[[Category: Scheffzek, K.]]	+	[[Category: Scheffzek, K]]
	[[Category: Dual activity]]		[[Category: Dual activity]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 16:36, 28 September 2014

2014-09-28T16:36:26Z

←Older revision		Revision as of 16:36, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ko7.~~png~~\|~~left~~\|200px]]	+	==X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution==
		+	<StructureSection load='1ko7' size='340' side='right' caption='[[1ko7]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KO7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1288 Staphylococcus xylosus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ko7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ko7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1ko7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression, mainly in Gram-positive bacteria. It is a bifunctional enzyme that phosphorylates Ser-46-HPr in an ATP-dependent reaction and dephosphorylates P-Ser-46-HPr. X-ray analysis of the full-length crystalline enzyme from Staphylococcus xylosus at a resolution of 1.95 A shows the enzyme to consist of two clearly separated domains that are assembled in a hexameric structure resembling a three-bladed propeller. The N-terminal domain has a betaalphabeta fold similar to a segment from enzyme I of the sugar phosphotransferase system and to the uridyl-binding portion of MurF; it is structurally organized in three dimeric modules exposed to form the propeller blades. Two unexpected phosphate ions associated with highly conserved residues were found in the N-terminal dimeric interface. The C-terminal kinase domain is similar to that of the Lactobacillus casei enzyme and is assembled in six copies to form the compact central hub of the propeller. Beyond previously reported similarity with adenylate kinase, we suggest evolutionary relationship with phosphoenolpyruvate carboxykinase. In addition to a phosphate ion in the phosphate-binding loop of the kinase domain, we have identified a second phosphate-binding site that, by comparison with adenylate kinases, we believe accommodates a product/substrate phosphate, normally covalently linked to Ser-46 of HPr. Thus, we propose that our structure represents a product/substrate mimic of the kinase/phosphatase reaction.

-	~~{{STRUCTURE_1ko7\| PDB=1ko7 \| SCENE= }}~~	+	Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions.,Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:11904409<ref>PMID:11904409</ref>

-	~~===X-ray structure~~ of the ~~HPr kinase/phosphatase from Staphylococcus xylosus at 1~~.~~95 A resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11904409}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ko7]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011904409<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus xylosus]]		[[Category: Staphylococcus xylosus]]
	[[Category: Fieulaine, S.]]		[[Category: Fieulaine, S.]]

OCA: Protected "1ko7" [edit=sysop:move=sysop]

2012-12-05T19:43:20Z

Protected "1ko7" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:43, 5 December 2012

OCA at 19:43, 5 December 2012

2012-12-05T19:43:16Z

←Older revision		Revision as of 19:43, 5 December 2012
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-	{{Seed}}
	[[Image:1ko7.png\|left\|200px]]		[[Image:1ko7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ko7", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ko7\| PDB=1ko7 \| SCENE= }}		{{STRUCTURE_1ko7\| PDB=1ko7 \| SCENE= }}

	===X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution===		===X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11904409}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11904409 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_11904409}}		{{ABSTRACT_PUBMED_11904409}}

	==About this Structure==		==About this Structure==
-	~~1KO7~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA].	+	[[1ko7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11904409~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011904409</ref><references group="xtra"/>
	[[Category: Staphylococcus xylosus]]		[[Category: Staphylococcus xylosus]]
	[[Category: Fieulaine, S.]]		[[Category: Fieulaine, S.]]
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	[[Category: Scheffzek, K.]]		[[Category: Scheffzek, K.]]
	[[Category: Dual activity]]		[[Category: Dual activity]]
		+	[[Category: Hydrolase]]
	[[Category: Phosphotransfer]]		[[Category: Phosphotransfer]]
	[[Category: Product]]		[[Category: Product]]
Line 38:		Line 27:
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]
	[[Category: Substrate]]		[[Category: Substrate]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 09:05:40 2009''~~	+

OCA at 07:05, 18 February 2009

2009-02-18T07:05:40Z

←Older revision		Revision as of 07:05, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1KO7 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA].	+	1KO7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_xylosus Staphylococcus xylosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KO7 OCA].

	==Reference==		==Reference==
-	Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions., Marquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K, Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11904409 11904409]~~	+	<ref group="xtra">PMID:11904409</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Staphylococcus xylosus]]		[[Category: Staphylococcus xylosus]]
	[[Category: Fieulaine, S.]]		[[Category: Fieulaine, S.]]
Line 40:		Line 39:
	[[Category: Substrate]]		[[Category: Substrate]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 2 10~~:38:~~10 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:05:40 2009''

OCA at 07:38, 2 July 2008

2008-07-02T07:38:14Z

←Older revision		Revision as of 07:38, 2 July 2008
Line 1:		Line 1:
-	[[Image:1ko7.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ko7.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1ko7\| PDB=1ko7 \| SCENE= }}		{{STRUCTURE_1ko7\| PDB=1ko7 \| SCENE= }}

-	~~'''~~X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution~~'''~~	+	===X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution===


-	~~==Overview==~~	+	<!--
-	The ~~histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression~~, ~~mainly in Gram-positive bacteria. It is a bifunctional enzyme that phosphorylates Ser-46-HPr in an ATP-dependent reaction and dephosphorylates P-Ser-46-HPr. X-ray analysis of~~ the ~~full-length crystalline enzyme from Staphylococcus xylosus at a resolution of 1.95 A shows the enzyme~~ to consist of two clearly separated domains that are assembled in a hexameric structure resembling a three-bladed propeller. The N-terminal domain has a betaalphabeta fold similar to a segment from enzyme I of the ~~sugar phosphotransferase system and to the uridyl-binding portion of MurF;~~ it ~~is structurally organized in three dimeric modules exposed to form the propeller blades~~. ~~Two unexpected phosphate ions associated with highly conserved residues were found in the N-terminal dimeric interface~~. ~~The C-terminal kinase domain~~ is ~~similar to that of~~ the ~~Lactobacillus casei enzyme and is assembled in six copies to form the compact central hub of the propeller~~. ~~Beyond previously reported similarity with adenylate kinase, we suggest evolutionary relationship with phosphoenolpyruvate carboxykinase. In addition to a phosphate ion in the phosphate~~-~~binding loop of the kinase domain, we have identified a second phosphate~~-binding site that, by comparison with adenylate kinases, we believe accommodates a product/substrate phosphate, normally covalently linked to Ser-46 of HPr. Thus, we propose that our structure represents a product/substrate mimic of the kinase/phosphatase reaction.	+	The line below this paragraph, {{ABSTRACT_PUBMED_11904409}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 11904409 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_11904409}}

	==About this Structure==		==About this Structure==
Line 35:		Line 39:
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]
	[[Category: Substrate]]		[[Category: Substrate]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 22:58:29 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:38:10 2008''