1ld6 - Revision history

OCA at 08:08, 3 April 2024

2024-04-03T08:08:26Z

←Older revision		Revision as of 08:08, 3 April 2024
Line 1:		Line 1:

	==STRUCTURE OF BPTI_8A MUTANT==		==STRUCTURE OF BPTI_8A MUTANT==
-	<StructureSection load='1ld6' size='340' side='right'caption='[[1ld6~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1ld6' size='340' side='right'caption='[[1ld6]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [https://pdbe.org/1ld6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ld6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ld6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [https://pdbe.org/1ld6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ld6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ld6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.	+	[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ld6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ld6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Here we determined NMR solution structures of two mutants of bovine pancreatic trypsin inhibitor (BPTI) to reveal structural reasons of their decreased thermodynamic stability. A point mutation, A16V, in the solvent-exposed loop destabilizes the protein by 20 degrees C, in contrast to marginal destabilization observed for G, S, R, L or W mutants. In the second mutant introduction of eight alanine residues at proteinase-contacting sites (residues 11, 13, 17, 18, 19, 34, 37 and 39) provides a protein that denatures at a temperature about 30 degrees C higher than expected from additive behavior of individual mutations. In order to efficiently determine structures of these variants, we applied a procedure that allows us to share data between regions unaffected by mutation(s). NOAH/DYANA and CNS programs were used for a rapid assignment of NOESY cross-peaks, structure calculations and refinement. The solution structure of the A16V mutant reveals no conformational change within the molecule, but shows close contacts between V16, I18 and G36/G37. Thus, the observed 4.3kcal/mol decrease of stability results from a strained local conformation of these residues caused by introduction of a beta-branched Val side-chain. Contrary to the A16V mutation, introduction of eight alanine residues produces significant conformational changes, manifested in over a 9A shift of the Y35 side-chain. This structural rearrangement provides about 6kcal/mol non-additive stabilization energy, compared to the mutant in which G37 and R39 are not mutated to alanine residues.
-
-	NMR structures of two variants of bovine pancreatic trypsin inhibitor (BPTI) reveal unexpected influence of mutations on protein structure and stability.,Cierpicki T, Otlewski J J Mol Biol. 2002 Aug 23;321(4):647-58. PMID:12206780<ref>PMID:12206780</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[BPTI 3D structures\|BPTI 3D structures]]		*[[BPTI 3D structures\|BPTI 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cierpicki, T]]	+	[[Category: Cierpicki T]]
-	[[Category: Otlewski, J]]	+	[[Category: Otlewski J]]
-	~~[[Category: Bpti]]~~	+
-	~~[[Category: Hydrolase inhibitor]]~~	+
-	~~[[Category: Kunitz fold~~]]	+

OCA at 06:42, 18 August 2021

2021-08-18T06:42:56Z

←Older revision		Revision as of 06:42, 18 August 2021
Line 3:		Line 3:
	<StructureSection load='1ld6' size='340' side='right'caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ld6' size='340' side='right'caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [~~http~~://pdbe.org/1ld6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ld6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ld6 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [https://pdbe.org/1ld6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ld6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ld6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.	+	[[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:05, 13 November 2019

2019-11-13T12:05:28Z

←Older revision		Revision as of 12:05, 13 November 2019
Line 1:		Line 1:

	==STRUCTURE OF BPTI_8A MUTANT==		==STRUCTURE OF BPTI_8A MUTANT==
-	<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1ld6' size='340' side='right'caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[BPTI 3D structures\|BPTI 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Cierpicki, T]]		[[Category: Cierpicki, T]]
	[[Category: Otlewski, J]]		[[Category: Otlewski, J]]

OCA at 21:15, 24 January 2018

2018-01-24T21:15:31Z

←Older revision		Revision as of 21:15, 24 January 2018
Line 1:		Line 1:
		+
	==STRUCTURE OF BPTI_8A MUTANT==		==STRUCTURE OF BPTI_8A MUTANT==
	<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://pdbe.org/1ld6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://pdbe.org/1ld6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ld6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ld6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ld6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:51, 7 February 2016

2016-02-07T01:51:31Z

←Older revision		Revision as of 01:51, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ld6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:32, 9 September 2015

2015-09-09T20:32:23Z

←Older revision		Revision as of 20:32, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://pdbe.org/1ld6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ld6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: Cierpicki, T]]		[[Category: Cierpicki, T]]
	[[Category: Otlewski, J]]		[[Category: Otlewski, J]]

OCA at 06:48, 24 December 2014

2014-12-24T06:48:25Z

←Older revision		Revision as of 06:48, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Cierpicki, T.]]	+	[[Category: Cierpicki, T]]
-	[[Category: Otlewski, J.]]	+	[[Category: Otlewski, J]]
	[[Category: Bpti]]		[[Category: Bpti]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]
	[[Category: Kunitz fold]]		[[Category: Kunitz fold]]

OCA at 13:06, 28 September 2014

2014-09-28T13:06:00Z

←Older revision		Revision as of 13:06, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1ld6.~~png\|left~~\|200px]]	+	==STRUCTURE OF BPTI_8A MUTANT==
		+	<StructureSection load='1ld6' size='340' side='right' caption='[[1ld6]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LD6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ld6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ld6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ld6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ld6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ld/1ld6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Here we determined NMR solution structures of two mutants of bovine pancreatic trypsin inhibitor (BPTI) to reveal structural reasons of their decreased thermodynamic stability. A point mutation, A16V, in the solvent-exposed loop destabilizes the protein by 20 degrees C, in contrast to marginal destabilization observed for G, S, R, L or W mutants. In the second mutant introduction of eight alanine residues at proteinase-contacting sites (residues 11, 13, 17, 18, 19, 34, 37 and 39) provides a protein that denatures at a temperature about 30 degrees C higher than expected from additive behavior of individual mutations. In order to efficiently determine structures of these variants, we applied a procedure that allows us to share data between regions unaffected by mutation(s). NOAH/DYANA and CNS programs were used for a rapid assignment of NOESY cross-peaks, structure calculations and refinement. The solution structure of the A16V mutant reveals no conformational change within the molecule, but shows close contacts between V16, I18 and G36/G37. Thus, the observed 4.3kcal/mol decrease of stability results from a strained local conformation of these residues caused by introduction of a beta-branched Val side-chain. Contrary to the A16V mutation, introduction of eight alanine residues produces significant conformational changes, manifested in over a 9A shift of the Y35 side-chain. This structural rearrangement provides about 6kcal/mol non-additive stabilization energy, compared to the mutant in which G37 and R39 are not mutated to alanine residues.

-	~~{{STRUCTURE_1ld6\| PDB=1ld6 \| SCENE= }}~~	+	NMR structures of two variants of bovine pancreatic trypsin inhibitor (BPTI) reveal unexpected influence of mutations on protein structure and stability.,Cierpicki T, Otlewski J J Mol Biol. 2002 Aug 23;321(4):647-58. PMID:12206780<ref>PMID:12206780</ref>

-	~~===STRUCTURE OF BPTI_8A MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12206780}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ld6]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA].~~	+

	==See Also==		==See Also==
	*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]		*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012206780<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Cierpicki, T.]]		[[Category: Cierpicki, T.]]

OCA at 05:16, 20 October 2012

2012-10-20T05:16:12Z

←Older revision		Revision as of 05:16, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1ld6]] is a 1 chain structure ~~of [[Basic Pancreatic Trypsin Inhibitor]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA].	+	[[1ld6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LD6 OCA].

	==See Also==		==See Also==

OCA at 09:31, 25 July 2012

2012-07-25T09:31:24Z

←Older revision		Revision as of 09:31, 25 July 2012
Line 1:		Line 1:
	[[Image:1ld6.png\|left\|200px]]		[[Image:1ld6.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ld6", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ld6\| PDB=1ld6 \| SCENE= }}		{{STRUCTURE_1ld6\| PDB=1ld6 \| SCENE= }}

	===STRUCTURE OF BPTI_8A MUTANT===		===STRUCTURE OF BPTI_8A MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12206780}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12206780 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12206780}}		{{ABSTRACT_PUBMED_12206780}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Basic Pancreatic Trypsin Inhibitor]]	+	*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12206780~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012206780</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Cierpicki, T.]]		[[Category: Cierpicki, T.]]