1les - Revision history

OCA at 07:32, 14 February 2024

2024-02-14T07:32:31Z

←Older revision		Revision as of 07:32, 14 February 2024
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	<StructureSection load='1les' size='340' side='right'caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1les' size='340' side='right'caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1LES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LES FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=~~FRU~~:~~FRUCTOSE~~'>~~FRU~~</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [~~http~~://pdbe.org/1les PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1les PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [https://pdbe.org/1les PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [https://www.ebi.ac.uk/pdbsum/1les PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LEC_LENCU LEC_LENCU]] D-mannose specific lectin (By similarity).	+	[https://www.uniprot.org/uniprot/LEC_LENCU LEC_LENCU] D-mannose specific lectin (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1les ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1les ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The conformational features of sucrose in the combining site of lentil lectin have been characterized through elucidation of a crystalline complex at 1.9-A resolution, transferred nuclear Overhauser effect experiments performed at 600 Mhz, and molecular modeling. In the crystal, the lentil lectin dimer binds one sucrose molecule per monomer. The locations of 229 water molecules have been identified. NMR experiments have provided 11 transferred NOEs. In parallel, the docking study and conformational analysis of sucrose in the combining site of lentil lectin indicate that three different conformations can be accommodated. Of these, the orientation with lowest energy is identical with the one observed in the crystalline complex and provides good agreement with the observed transferred NOEs. These structural investigations indicate that the bound sucrose has a unique conformation for the glycosidic linkage, close to the one observed in crystalline sucrose, whereas the fructofuranose ring remains relatively flexible and does not exhibit any strong interaction with the protein. Major differences in the hydrogen bonding network of sucrose are found. None of the two inter-residue hydrogen bonds in crystalline sucrose are conserved in the complex with the lectin. Instead, a water molecule bridges hydroxyl groups O2-g and O3-f of sucrose.
-
-	NMR, molecular modeling, and crystallographic studies of lentil lectin-sucrose interaction.,Casset F, Hamelryck T, Loris R, Brisson JR, Tellier C, Dao-Thi MH, Wyns L, Poortmans F, Perez S, Imberty A J Biol Chem. 1995 Oct 27;270(43):25619-28. PMID:7592736<ref>PMID:7592736</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1les" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lens culinaris]]		[[Category: Lens culinaris]]
-	[[Category: Hamelryck, T]]	+	[[Category: Hamelryck T]]
-	[[Category: Loris, R]]	+	[[Category: Loris R]]
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Protein-sugar interaction~~]]	+

OCA at 05:54, 14 October 2020

2020-10-14T05:54:07Z

←Older revision		Revision as of 05:54, 14 October 2020
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	<StructureSection load='1les' size='340' side='right'caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1les' size='340' side='right'caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1LES FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA~~</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU~~</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:17, 22 January 2020

2020-01-22T17:17:53Z

←Older revision		Revision as of 17:17, 22 January 2020
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	==LENTIL LECTIN COMPLEXED WITH SUCROSE==		==LENTIL LECTIN COMPLEXED WITH SUCROSE==
-	<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1les' size='340' side='right'caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>		<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lens culinaris]]		[[Category: Lens culinaris]]
	[[Category: Hamelryck, T]]		[[Category: Hamelryck, T]]

OCA at 06:00, 18 April 2018

2018-04-18T06:00:22Z

←Older revision		Revision as of 06:00, 18 April 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1les_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1les_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:44, 29 November 2017

2017-11-29T06:44:25Z

←Older revision		Revision as of 06:44, 29 November 2017
Line 3:		Line 3:
	<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cicer_lens Cicer lens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cicer lens~~]]	+	[[Category: Lens culinaris]]
	[[Category: Hamelryck, T]]		[[Category: Hamelryck, T]]
	[[Category: Loris, R]]		[[Category: Loris, R]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Protein-sugar interaction]]		[[Category: Protein-sugar interaction]]

OCA at 10:38, 18 October 2017

2017-10-18T10:38:46Z

←Older revision		Revision as of 10:38, 18 October 2017
Line 1:		Line 1:
		+
	==LENTIL LECTIN COMPLEXED WITH SUCROSE==		==LENTIL LECTIN COMPLEXED WITH SUCROSE==
	<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lens_culinaris Lens culinaris~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cicer_lens Cicer lens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN~~</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1les ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1les ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lens culinaris~~]]	+	[[Category: Cicer lens]]
	[[Category: Hamelryck, T]]		[[Category: Hamelryck, T]]
	[[Category: Loris, R]]		[[Category: Loris, R]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Protein-sugar interaction]]		[[Category: Protein-sugar interaction]]

OCA at 12:45, 11 September 2015

2015-09-11T12:45:42Z

←Older revision		Revision as of 12:45, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>		<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://pdbe.org/1les PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1les" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:38, 25 December 2014

2014-12-25T16:38:27Z

←Older revision		Revision as of 16:38, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>		<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEC_LENCU LEC_LENCU]] D-mannose specific lectin (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Lens culinaris]]		[[Category: Lens culinaris]]
-	[[Category: Hamelryck, T.]]	+	[[Category: Hamelryck, T]]
-	[[Category: Loris, R.]]	+	[[Category: Loris, R]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Protein-sugar interaction]]		[[Category: Protein-sugar interaction]]

OCA at 14:52, 28 September 2014

2014-09-28T14:52:42Z

←Older revision		Revision as of 14:52, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1les.~~png\|left~~\|200px]]	+	==LENTIL LECTIN COMPLEXED WITH SUCROSE==
		+	<StructureSection load='1les' size='340' side='right' caption='[[1les]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1les]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lens_culinaris Lens culinaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LES FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1les FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1les OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1les RCSB], [http://www.ebi.ac.uk/pdbsum/1les PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/le/1les_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The conformational features of sucrose in the combining site of lentil lectin have been characterized through elucidation of a crystalline complex at 1.9-A resolution, transferred nuclear Overhauser effect experiments performed at 600 Mhz, and molecular modeling. In the crystal, the lentil lectin dimer binds one sucrose molecule per monomer. The locations of 229 water molecules have been identified. NMR experiments have provided 11 transferred NOEs. In parallel, the docking study and conformational analysis of sucrose in the combining site of lentil lectin indicate that three different conformations can be accommodated. Of these, the orientation with lowest energy is identical with the one observed in the crystalline complex and provides good agreement with the observed transferred NOEs. These structural investigations indicate that the bound sucrose has a unique conformation for the glycosidic linkage, close to the one observed in crystalline sucrose, whereas the fructofuranose ring remains relatively flexible and does not exhibit any strong interaction with the protein. Major differences in the hydrogen bonding network of sucrose are found. None of the two inter-residue hydrogen bonds in crystalline sucrose are conserved in the complex with the lectin. Instead, a water molecule bridges hydroxyl groups O2-g and O3-f of sucrose.

-	~~{{STRUCTURE_1les\| PDB=1les \| SCENE= }}~~	+	NMR, molecular modeling, and crystallographic studies of lentil lectin-sucrose interaction.,Casset F, Hamelryck T, Loris R, Brisson JR, Tellier C, Dao-Thi MH, Wyns L, Poortmans F, Perez S, Imberty A J Biol Chem. 1995 Oct 27;270(43):25619-28. PMID:7592736<ref>PMID:7592736</ref>

-	~~===LENTIL LECTIN COMPLEXED WITH SUCROSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7592736}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1les]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lens_culinaris Lens culinaris]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LES OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007592736<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Lens culinaris]]		[[Category: Lens culinaris]]
	[[Category: Hamelryck, T.]]		[[Category: Hamelryck, T.]]

OCA: Protected "1les" [edit=sysop:move=sysop]

2012-12-05T20:40:18Z

Protected "1les" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:40, 5 December 2012