1lqi - Revision history

OCA at 08:26, 10 April 2024

2024-04-10T08:26:22Z

←Older revision		Revision as of 08:26, 10 April 2024
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	==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==		==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==
-	<StructureSection load='1lqi' size='340' side='right'caption='[[1lqi~~]], [[NMR_Ensembles_of_Models \| 29 NMR models~~]]' scene=''>	+	<StructureSection load='1lqi' size='340' side='right'caption='[[1lqi]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Deathstalker_scorpion Deathstalker scorpion~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1lqh\|1lqh]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Deathstalker scorpion])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [https://pdbe.org/1lqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [https://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [https://pdbe.org/1lqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [https://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~SCXA_LEIQH SCXA_LEIQH]~~] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.	+	[https://www.uniprot.org/uniprot/SCXA_LEIHE SCXA_LEIHE] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of a recombinant active alpha-neurotoxin from Leiurus quinquestriatus hebraeus, Lqh(alpha)IT, was determined by proton two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). This toxin is the most insecticidal among scorpion alpha-neurotoxins and, therefore, serves as a model for clarifying the structural basis for their biological activity and selective toxicity. A set of 29 structures was generated without constraint violations exceeding 0.4 A. These structures had root mean square deviations of 0.49 and 1.00 A with respect to the average structure for backbone atoms and all heavy atoms, respectively. Similarly to other scorpion toxins, the structure of Lqh(alpha)IT consists of an alpha-helix, a three-strand antiparallel beta-sheet, three type I tight turns, a five-residue turn, and a hydrophobic patch that includes tyrosine and tryptophan rings in a "herringbone" arrangement. Positive phi angles were found for Ala50 and Asn11, suggesting their proximity to functionally important regions of the molecule. The sample exhibited conformational heterogeneity over a wide range of experimental conditions, and two conformations were observed for the majority of protein residues. The ratio between these conformations was temperature-dependent, and the rate of their interconversions was estimated to be on the order of 1-5 s(-1) at 308 K. The conformation of the polypeptide backbone of Lqh(alpha)IT is very similar to that of the most active antimammalian scorpion alpha-toxin, AaHII, from Androctonus australis Hector (60% amino acid sequence homology). Yet, several important differences were observed at the 5-residue turn comprising residues Lys8-Cys12, the C-terminal segment, and the mutual disposition of these two regions. 2D NMR studies of the R64H mutant, which is 3 times more toxic than the unmodified Lqh(alpha)IT, demonstrated the importance of the spatial orientation of the last residue side chain for toxicity of Lqh(alpha)IT.
-
-	Solution structures of a highly insecticidal recombinant scorpion alpha-toxin and a mutant with increased activity.,Tugarinov V, Kustanovich I, Zilberberg N, Gurevitz M, Anglister J Biochemistry. 1997 Mar 4;36(9):2414-24. PMID:9054546<ref>PMID:9054546</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]		*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
	*[[Potassium channel toxin 3D structures\|Potassium channel toxin 3D structures]]		*[[Potassium channel toxin 3D structures\|Potassium channel toxin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Deathstalker scorpion]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anglister, J]]	+	[[Category: Leiurus quinquestriatus hebraeus]]
-	[[Category: Gurevitz, M]]	+	[[Category: Anglister J]]
-	[[Category: Kustanovich, I]]	+	[[Category: Gurevitz M]]
-	[[Category: Tugarinov, V]]	+	[[Category: Kustanovich I]]
-	[[Category: Zilberberg, N]]	+	[[Category: Tugarinov V]]
-	~~[[Category: Neurotoxin]]~~	+	[[Category: Zilberberg N]]
-	~~[[Category: Sodium channel inhibitor~~]]	+

OCA at 08:14, 23 February 2022

2022-02-23T08:14:31Z

←Older revision		Revision as of 08:14, 23 February 2022
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	<StructureSection load='1lqi' size='340' side='right'caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1lqi' size='340' side='right'caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Leiqh Leiqh~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1LQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deathstalker_scorpion Deathstalker scorpion]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lqh\|1lqh]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lqh\|1lqh]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 ~~LEIQH~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Deathstalker scorpion])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [~~http~~://pdbe.org/1lqi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [https://pdbe.org/1lqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [https://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SCXA_LEIQH SCXA_LEIQH]] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.	+	[[https://www.uniprot.org/uniprot/SCXA_LEIQH SCXA_LEIQH]] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Deathstalker scorpion]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Leiqh]]
	[[Category: Anglister, J]]		[[Category: Anglister, J]]
	[[Category: Gurevitz, M]]		[[Category: Gurevitz, M]]

OCA at 08:01, 25 November 2020

2020-11-25T08:01:05Z

←Older revision		Revision as of 08:01, 25 November 2020
Line 1:		Line 1:

	==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==		==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==
-	<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>	+	<StructureSection load='1lqi' size='340' side='right'caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiqh Leiqh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1LQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiqh Leiqh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lqh\|1lqh]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://pdbe.org/1lqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://pdbe.org/1lqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemolysin 3D structures\|Hemolysin 3D structures]]
		+	*[[Potassium channel toxin 3D structures\|Potassium channel toxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Leiqh]]		[[Category: Leiqh]]
	[[Category: Anglister, J]]		[[Category: Anglister, J]]

OCA at 08:04, 31 January 2018

2018-01-31T08:04:34Z

←Older revision		Revision as of 08:04, 31 January 2018
Line 1:		Line 1:
		+
	==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==		==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==
	<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://pdbe.org/1lqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://pdbe.org/1lqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Potassium channel toxin\|Potassium channel toxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:51, 8 February 2016

2016-02-08T18:51:23Z

←Older revision		Revision as of 18:51, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:25, 10 September 2015

2015-09-10T14:25:22Z

←Older revision		Revision as of 14:25, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>		<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiqh Leiqh]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 ~~Leiurus quinquestriatus hebraeus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 LEIQH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://pdbe.org/1lqi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1lqi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Leiurus quinquestriatus hebraeus~~]]	+	[[Category: Leiqh]]
	[[Category: Anglister, J]]		[[Category: Anglister, J]]
	[[Category: Gurevitz, M]]		[[Category: Gurevitz, M]]

OCA at 01:26, 25 December 2014

2014-12-25T01:26:19Z

←Older revision		Revision as of 01:26, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Leiurus quinquestriatus hebraeus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Leiurus quinquestriatus hebraeus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SCXA_LEIQH SCXA_LEIQH]] Alpha toxins bind voltage-independently at site-3 of sodium channels (Nav) and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. The dissociation is voltage-dependent. This toxin is active on insects. It is also highly toxic to crustaceans and has a measurable but low toxicity to mice.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Leiurus quinquestriatus hebraeus]]		[[Category: Leiurus quinquestriatus hebraeus]]
-	[[Category: Anglister, J.]]	+	[[Category: Anglister, J]]
-	[[Category: Gurevitz, M.]]	+	[[Category: Gurevitz, M]]
-	[[Category: Kustanovich, I.]]	+	[[Category: Kustanovich, I]]
-	[[Category: Tugarinov, V.]]	+	[[Category: Tugarinov, V]]
-	[[Category: Zilberberg, N.]]	+	[[Category: Zilberberg, N]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
	[[Category: Sodium channel inhibitor]]		[[Category: Sodium channel inhibitor]]

OCA at 16:10, 28 September 2014

2014-09-28T16:10:47Z

←Older revision		Revision as of 16:10, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1lqi.~~png~~\|~~left~~\|200px]]	+	==INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES==
		+	<StructureSection load='1lqi' size='340' side='right' caption='[[1lqi]], [[NMR_Ensembles_of_Models \| 29 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LQI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lqh\|1lqh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6884 Leiurus quinquestriatus hebraeus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lqi RCSB], [http://www.ebi.ac.uk/pdbsum/1lqi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of a recombinant active alpha-neurotoxin from Leiurus quinquestriatus hebraeus, Lqh(alpha)IT, was determined by proton two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). This toxin is the most insecticidal among scorpion alpha-neurotoxins and, therefore, serves as a model for clarifying the structural basis for their biological activity and selective toxicity. A set of 29 structures was generated without constraint violations exceeding 0.4 A. These structures had root mean square deviations of 0.49 and 1.00 A with respect to the average structure for backbone atoms and all heavy atoms, respectively. Similarly to other scorpion toxins, the structure of Lqh(alpha)IT consists of an alpha-helix, a three-strand antiparallel beta-sheet, three type I tight turns, a five-residue turn, and a hydrophobic patch that includes tyrosine and tryptophan rings in a "herringbone" arrangement. Positive phi angles were found for Ala50 and Asn11, suggesting their proximity to functionally important regions of the molecule. The sample exhibited conformational heterogeneity over a wide range of experimental conditions, and two conformations were observed for the majority of protein residues. The ratio between these conformations was temperature-dependent, and the rate of their interconversions was estimated to be on the order of 1-5 s(-1) at 308 K. The conformation of the polypeptide backbone of Lqh(alpha)IT is very similar to that of the most active antimammalian scorpion alpha-toxin, AaHII, from Androctonus australis Hector (60% amino acid sequence homology). Yet, several important differences were observed at the 5-residue turn comprising residues Lys8-Cys12, the C-terminal segment, and the mutual disposition of these two regions. 2D NMR studies of the R64H mutant, which is 3 times more toxic than the unmodified Lqh(alpha)IT, demonstrated the importance of the spatial orientation of the last residue side chain for toxicity of Lqh(alpha)IT.

-	~~{{STRUCTURE_1lqi\| PDB=1lqi \| SCENE= }}~~	+	Solution structures of a highly insecticidal recombinant scorpion alpha-toxin and a mutant with increased activity.,Tugarinov V, Kustanovich I, Zilberberg N, Gurevitz M, Anglister J Biochemistry. 1997 Mar 4;36(9):2414-24. PMID:9054546<ref>PMID:9054546</ref>

-	~~===INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS~~, ~~NMR, 29 STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9054546}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1lqi]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Scorpion~~ toxin\|~~Scorpion~~ toxin]]	+	*[[Potassium channel toxin\|Potassium channel toxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009054546<~~/~~ref~~><~~ref group="xtra">PMID:017877370</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Leiurus quinquestriatus hebraeus]]		[[Category: Leiurus quinquestriatus hebraeus]]
	[[Category: Anglister, J.]]		[[Category: Anglister, J.]]

OCA: Protected "1lqi" [edit=sysop:move=sysop]

2012-10-20T11:07:40Z

Protected "1lqi" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:07, 20 October 2012

OCA at 11:07, 20 October 2012

2012-10-20T11:07:36Z

←Older revision		Revision as of 11:07, 20 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1lqi.png\|left\|200px]]		[[Image:1lqi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1lqi", creates the "Structure Box" on the page.
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	{{STRUCTURE_1lqi\| PDB=1lqi \| SCENE= }}		{{STRUCTURE_1lqi\| PDB=1lqi \| SCENE= }}

	===INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES===		===INSECTICIDAL ALPHA SCORPION TOXIN ISOLATED FROM THE VENOM OF SCORPION LEIURUS QUINQUESTRIATUS HEBRAEUS, NMR, 29 STRUCTURES===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9054546}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9054546 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9054546}}		{{ABSTRACT_PUBMED_9054546}}

	==About this Structure==		==About this Structure==
-	~~1LQI~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA].	+	[[1lqi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiurus_quinquestriatus_hebraeus Leiurus quinquestriatus hebraeus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQI OCA].
		+
		+	==See Also==
		+	*[[Scorpion toxin\|Scorpion toxin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9054546~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009054546</ref><ref group="xtra">PMID:017877370</ref><references group="xtra"/>
	[[Category: Leiurus quinquestriatus hebraeus]]		[[Category: Leiurus quinquestriatus hebraeus]]
	[[Category: Anglister, J.]]		[[Category: Anglister, J.]]
Line 31:		Line 22:
	[[Category: Zilberberg, N.]]		[[Category: Zilberberg, N.]]
	[[Category: Neurotoxin]]		[[Category: Neurotoxin]]
-	[[Category: Signal]]
	[[Category: Sodium channel inhibitor]]		[[Category: Sodium channel inhibitor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:54:39 2009''