1m8p - Revision history

OCA at 07:41, 14 February 2024

2024-02-14T07:41:31Z

←Older revision		Revision as of 07:41, 14 February 2024
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	<StructureSection load='1m8p' size='340' side='right'caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1m8p' size='340' side='right'caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_10106 Atcc 10106~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PPS:3~~-~~PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [https://pdbe.org/1m8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [https://www.ebi.ac.uk/pdbsum/1m8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [https://pdbe.org/1m8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [https://www.ebi.ac.uk/pdbsum/1m8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MET3_PENCH MET3_PENCH]] Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.	+	[https://www.uniprot.org/uniprot/MET3_PENCH MET3_PENCH] Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8p ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The structure of the cooperative hexameric enzyme ATP sulfurylase from Penicillium chrysogenum bound to its allosteric inhibitor, 3'-phosphoadenosine-5'-phosphosulfate (PAPS), was determined to 2.6 A resolution. This structure represents the low substrate-affinity T-state conformation of the enzyme. Comparison with the high substrate-affinity R-state structure reveals that a large rotational rearrangement of domains occurs as a result of the R-to-T transition. The rearrangement is accompanied by the 17 A movement of a 10-residue loop out of the active site region, resulting in an open, product release-like structure of the catalytic domain. Binding of PAPS is proposed to induce the allosteric transition by destabilizing an R-state-specific salt linkage between Asp 111 in an N-terminal domain of one subunit and Arg 515 in the allosteric domain of a trans-triad subunit. Disrupting this salt linkage by site-directed mutagenesis induces cooperative inhibition behavior in the absence of an allosteric effector, confirming the role of these two residues.
-
-	Allosteric inhibition via R-state destabilization in ATP sulfurylase from Penicillium chrysogenum.,MacRae IJ, Segel IH, Fisher AJ Nat Struct Biol. 2002 Dec;9(12):945-9. PMID:12426581<ref>PMID:12426581</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1m8p" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 10106]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sulfate adenylyltransferase~~]]	+	[[Category: Penicillium chrysogenum]]
-	[[Category: Fisher~~, A J~~]]	+	[[Category: Fisher AJ]]
-	[[Category: MacRae~~, I J~~]]	+	[[Category: MacRae IJ]]
-	[[Category: Segel~~, I H]]~~	+	[[Category: Segel IH]]
-	~~[[Category: Phosphosulfate binding]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: T-state]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:54, 18 August 2021

2021-08-18T06:54:05Z

←Older revision		Revision as of 06:54, 18 August 2021
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	<StructureSection load='1m8p' size='340' side='right'caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1m8p' size='340' side='right'caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_10106 Atcc 10106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1M8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_10106 Atcc 10106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [~~http~~://pdbe.org/1m8p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1m8p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [https://pdbe.org/1m8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [https://www.ebi.ac.uk/pdbsum/1m8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MET3_PENCH MET3_PENCH]] Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.	+	[[https://www.uniprot.org/uniprot/MET3_PENCH MET3_PENCH]] Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:30, 13 November 2019

2019-11-13T12:30:50Z

←Older revision		Revision as of 12:30, 13 November 2019
Line 1:		Line 1:

	==Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state==		==Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state==
-	<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='1m8p' size='340' side='right'caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10106 Atcc 10106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10106 Atcc 10106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 10106]]		[[Category: Atcc 10106]]
		+	[[Category: Large Structures]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]

OCA at 08:05, 31 January 2018

2018-01-31T08:05:59Z

←Older revision		Revision as of 08:05, 31 January 2018
Line 1:		Line 1:
		+
	==Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state==		==Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state==
	<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cbs_306.48 Cbs 306.48~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10106 Atcc 10106]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://pdbe.org/1m8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://pdbe.org/1m8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cbs 306 48~~]]	+	[[Category: Atcc 10106]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]

OCA at 03:16, 9 February 2016

2016-02-09T03:16:26Z

←Older revision		Revision as of 03:16, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:04, 10 September 2015

2015-09-10T18:04:33Z

←Older revision		Revision as of 18:04, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Penicillium_chrysogenum Penicillium chrysogenum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_306.48 Cbs 306.48]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://pdbe.org/1m8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1m8p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Penicillium chrysogenum~~]]	+	[[Category: Cbs 306 48]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]
	[[Category: Fisher, A J]]		[[Category: Fisher, A J]]

OCA at 04:02, 25 December 2014

2014-12-25T04:02:07Z

←Older revision		Revision as of 04:02, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MET3_PENCH MET3_PENCH]] Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Penicillium chrysogenum]]		[[Category: Penicillium chrysogenum]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]
-	[[Category: Fisher, A J.]]	+	[[Category: Fisher, A J]]
-	[[Category: MacRae, I J.]]	+	[[Category: MacRae, I J]]
-	[[Category: Segel, I H.]]	+	[[Category: Segel, I H]]
	[[Category: Phosphosulfate binding]]		[[Category: Phosphosulfate binding]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: T-state]]		[[Category: T-state]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:37, 28 September 2014

2014-09-28T14:37:04Z

←Older revision		Revision as of 14:37, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1m8p.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state==
		+	<StructureSection load='1m8p' size='340' side='right' caption='[[1m8p]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1M8P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPS:3-PHOSPHATE-ADENOSINE-5-PHOSPHATE+SULFATE'>PPS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1i2d\|1i2d]], [[1g8g\|1g8g]], [[1jhd\|1jhd]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1m8p RCSB], [http://www.ebi.ac.uk/pdbsum/1m8p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structure of the cooperative hexameric enzyme ATP sulfurylase from Penicillium chrysogenum bound to its allosteric inhibitor, 3'-phosphoadenosine-5'-phosphosulfate (PAPS), was determined to 2.6 A resolution. This structure represents the low substrate-affinity T-state conformation of the enzyme. Comparison with the high substrate-affinity R-state structure reveals that a large rotational rearrangement of domains occurs as a result of the R-to-T transition. The rearrangement is accompanied by the 17 A movement of a 10-residue loop out of the active site region, resulting in an open, product release-like structure of the catalytic domain. Binding of PAPS is proposed to induce the allosteric transition by destabilizing an R-state-specific salt linkage between Asp 111 in an N-terminal domain of one subunit and Arg 515 in the allosteric domain of a trans-triad subunit. Disrupting this salt linkage by site-directed mutagenesis induces cooperative inhibition behavior in the absence of an allosteric effector, confirming the role of these two residues.

-	~~{{STRUCTURE_1m8p\| PDB=1m8p \| SCENE= }}~~	+	Allosteric inhibition via R-state destabilization in ATP sulfurylase from Penicillium chrysogenum.,MacRae IJ, Segel IH, Fisher AJ Nat Struct Biol. 2002 Dec;9(12):945-9. PMID:12426581<ref>PMID:12426581</ref>

-	~~===Crystal Structure~~ of ~~P. chrysogenum ATP Sulfurylase in~~ the ~~T-state===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12426581}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1m8p]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012426581<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Penicillium chrysogenum]]		[[Category: Penicillium chrysogenum]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]

OCA: Protected "1m8p" [edit=sysop:move=sysop]

2012-12-05T20:14:17Z

Protected "1m8p" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:14, 5 December 2012

OCA at 20:14, 5 December 2012

2012-12-05T20:14:15Z

←Older revision		Revision as of 20:14, 5 December 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1m8p.png\|left\|200px]]		[[Image:1m8p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1m8p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1m8p\| PDB=1m8p \| SCENE= }}		{{STRUCTURE_1m8p\| PDB=1m8p \| SCENE= }}

	===Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state===		===Crystal Structure of P. chrysogenum ATP Sulfurylase in the T-state===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12426581}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12426581 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12426581}}		{{ABSTRACT_PUBMED_12426581}}

	==About this Structure==		==About this Structure==
-	~~1M8P~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA].	+	[[1m8p]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Penicillium_chrysogenum Penicillium chrysogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12426581~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012426581</ref><references group="xtra"/>
	[[Category: Penicillium chrysogenum]]		[[Category: Penicillium chrysogenum]]
	[[Category: Sulfate adenylyltransferase]]		[[Category: Sulfate adenylyltransferase]]
Line 32:		Line 20:
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: T-state]]		[[Category: T-state]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 06:01:41 2009''~~	+