1mm9 - Revision history

OCA at 07:45, 14 February 2024

2024-02-14T07:45:37Z

←Older revision		Revision as of 07:45, 14 February 2024
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	<StructureSection load='1mm9' size='340' side='right'caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='1mm9' size='340' side='right'caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MRD:(4R)~~-~~2-METHYLPENTANE-2~~,~~4-DIOL'>MRD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.66Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;'>[[1swe\|1swe]]~~, ~~[[1mk5\|1mk5]], [[1mep\|1mep]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [https://pdbe.org/1mm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [https://www.ebi.ac.uk/pdbsum/1mm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [~~http~~://pdbe.org/1mm9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mm9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The RGD (arginine-glycine-aspartic acid) sequence is found in several important extracellular matrix proteins and serves as an adhesion ligand for members of the integrin family of cell-surface receptors. This sequence and flanking residues from fibronectin or osteopontin have been engineered into an accessible surface loop of streptavidin to create two new streptavidin variants (FN-SA or OPN-SA, respectively) that bind cells through the alpha(v)beta(3) and/or alpha(5)beta(1) integrin receptors. Their crystal structures confirm the design and construction of the mutants and provide evidence about the conformational dynamics of the RGD loops. The loops in the isomorphous crystal structures are involved in crystal-packing interactions and this stabilizes their structures. Even so, the loop in OPN-SA is slightly disordered and two of the residues are not seen in difference electron-density maps. Comparison with other experimentally determined structures of RGD loops in cell-adhesion molecules shows that these loops occupy a large subset of conformational space. This is consistent with the view that RGD loops, at least those involved in cell adhesion, sample a number of structures dynamically, a few of which display high affinity for appropriate receptors.
-
-	Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin.,Le Trong I, McDevitt TC, Nelson KE, Stayton PS, Stenkamp RE Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):828-34. Epub 2003, Apr 25. PMID:12777798<ref>PMID:12777798</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~McDevitt, T C~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Nelson, K E~~]]	+	[[Category: Le Trong I]]
-	[[Category: ~~Stayton, P S~~]]	+	[[Category: McDevitt TC]]
-	[[Category: ~~Stenkamp, R E~~]]	+	[[Category: Nelson KE]]
-	[[Category: ~~Trong, I Le~~]]	+	[[Category: Stayton PS]]
-	[[Category: ~~Biotin-binding protein]]~~	+	[[Category: Stenkamp RE]]
-	~~[[Category: Tetramer~~]]	+

OCA at 08:39, 2 December 2020

2020-12-02T08:39:04Z

←Older revision		Revision as of 08:39, 2 December 2020
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	==Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD==		==Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD==
-	<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>	+	<StructureSection load='1mm9' size='340' side='right'caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://pdbe.org/1mm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://pdbe.org/1mm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: McDevitt, T C]]		[[Category: McDevitt, T C]]
	[[Category: Nelson, K E]]		[[Category: Nelson, K E]]

OCA at 07:44, 31 January 2018

2018-01-31T07:44:25Z

←Older revision		Revision as of 07:44, 31 January 2018
Line 1:		Line 1:
		+
	==Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD==		==Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD==
	<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://pdbe.org/1mm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://pdbe.org/1mm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Avidin\|Avidin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:12, 8 February 2016

2016-02-08T16:12:38Z

←Older revision		Revision as of 16:12, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:16, 10 September 2015

2015-09-10T13:16:53Z

←Older revision		Revision as of 13:16, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 ~~Streptomyces avidinii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 AS 4.1583])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://pdbe.org/1mm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1mm9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: McDevitt, T C]]		[[Category: McDevitt, T C]]
	[[Category: Nelson, K E]]		[[Category: Nelson, K E]]

OCA at 00:33, 25 December 2014

2014-12-25T00:33:55Z

←Older revision		Revision as of 00:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 Streptomyces avidinii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 Streptomyces avidinii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: McDevitt, T C.]]	+	[[Category: McDevitt, T C]]
-	[[Category: Nelson, K E.]]	+	[[Category: Nelson, K E]]
-	[[Category: Stayton, P S.]]	+	[[Category: Stayton, P S]]
-	[[Category: Stenkamp, R E.]]	+	[[Category: Stenkamp, R E]]
-	[[Category: Trong, I Le.]]	+	[[Category: Trong, I Le]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]
	[[Category: Tetramer]]		[[Category: Tetramer]]

OCA at 15:26, 28 September 2014

2014-09-28T15:26:27Z

←Older revision		Revision as of 15:26, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1mm9.~~png~~\|~~left~~\|200px]]	+	==Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD==
		+	<StructureSection load='1mm9' size='340' side='right' caption='[[1mm9]], [[Resolution\|resolution]] 1.66Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MM9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]], [[1mk5\|1mk5]], [[1mep\|1mep]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">core streptavidin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1895 Streptomyces avidinii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mm9 RCSB], [http://www.ebi.ac.uk/pdbsum/1mm9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RGD (arginine-glycine-aspartic acid) sequence is found in several important extracellular matrix proteins and serves as an adhesion ligand for members of the integrin family of cell-surface receptors. This sequence and flanking residues from fibronectin or osteopontin have been engineered into an accessible surface loop of streptavidin to create two new streptavidin variants (FN-SA or OPN-SA, respectively) that bind cells through the alpha(v)beta(3) and/or alpha(5)beta(1) integrin receptors. Their crystal structures confirm the design and construction of the mutants and provide evidence about the conformational dynamics of the RGD loops. The loops in the isomorphous crystal structures are involved in crystal-packing interactions and this stabilizes their structures. Even so, the loop in OPN-SA is slightly disordered and two of the residues are not seen in difference electron-density maps. Comparison with other experimentally determined structures of RGD loops in cell-adhesion molecules shows that these loops occupy a large subset of conformational space. This is consistent with the view that RGD loops, at least those involved in cell adhesion, sample a number of structures dynamically, a few of which display high affinity for appropriate receptors.

-	~~{{STRUCTURE_1mm9\| PDB=1mm9 \| SCENE= }}~~	+	Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin.,Le Trong I, McDevitt TC, Nelson KE, Stayton PS, Stenkamp RE Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):828-34. Epub 2003, Apr 25. PMID:12777798<ref>PMID:12777798</ref>

-	~~===Streptavidin Mutant with Insertion of Fibronectin Hexapeptide~~, ~~including RGD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12777798}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1mm9]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_avidinii Streptomyces avidinii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012777798<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: McDevitt, T C.]]		[[Category: McDevitt, T C.]]

OCA at 10:43, 20 October 2012

2012-10-20T10:43:15Z

←Older revision		Revision as of 10:43, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1mm9]] is a 1 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA].	+	[[1mm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM9 OCA].

	==See Also==		==See Also==

OCA at 03:02, 26 July 2012

2012-07-26T03:02:24Z

←Older revision		Revision as of 03:02, 26 July 2012
Line 1:		Line 1:
	[[Image:1mm9.png\|left\|200px]]		[[Image:1mm9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1mm9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1mm9\| PDB=1mm9 \| SCENE= }}		{{STRUCTURE_1mm9\| PDB=1mm9 \| SCENE= }}

	===Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD===		===Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12777798}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12777798 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12777798}}		{{ABSTRACT_PUBMED_12777798}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12777798~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012777798</ref><references group="xtra"/>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: McDevitt, T C.]]		[[Category: McDevitt, T C.]]

OCA: Protected "1mm9" [edit=sysop:move=sysop]

2010-12-26T23:44:13Z

Protected "1mm9" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:44, 26 December 2010